Enterobacter bugandensis (DSM 29888, NCCB 100573, 247)

Name: Enterobacter bugandensis (DSM 29888, NCCB 100573, 247)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132404

Type strain

Strain Designation 247, 247BMC, EB-247

Culture col. no. DSM 29888 NCCB 100573 247 247BMC EB-247

NCBI tax ID(s) 881260

Special Collections DSMZ Literature strains DZIF

Links

Enterobacter bugandensis 247 is a facultative anaerobe, mesophilic, Gram-negative prokaryote that forms circular colonies and was isolated from human blood of a neonate.

Gram-negative motile rod-shaped colony-forming facultative anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 401827

NCCB 100573,DSM 29888,CCUG 75194T

@ref 20215

Last LPSN update 2025-12-16 10:01:30

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Enterobacterales

Family Enterobacteriaceae

Genus Enterobacter

Species Enterobacter bugandensis

Full scientific name Enterobacter bugandensis Doijad et al. 2016

BacDive ID	Other strains from **Enterobacter bugandensis** (8)	Type strain
132405	E. bugandensis 246, EB-246, DSM 101086
132406	E. bugandensis 248, EB-248, DSM 101087
132407	E. bugandensis 249, EB-249, DSM 101088
132408	E. bugandensis 250, EB-250, DSM 101089
132409	E. bugandensis 258, EB-258, DSM 101090
132410	E. bugandensis 260, EB-260, DSM 101091
132411	E. bugandensis 261, EB-261, DSM 101092
158205	E. bugandensis IF2SW-P2, DSM 104458

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape
43786	negative	2-3 µm	0.5 µm	rod-shaped
125439	negative
125438	negative

Colony morphology

@ref	Colony color	Colony shape	Incubation period	Medium used
43786	White	circular	1 day	Luria-Bertani agar
43786	White, opaque	circular	1 day	MacConkey agar

Culture and growth conditions

Culture medium

@ref	Name	Growth
43786	LB (Luria-Bertani) MEDIUM
43786	MacConkey agar
24193	BHI MEDIUM (DSMZ Medium 215)
24193	NUTRIENT AGAR (DSMZ Medium 1)

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
24193	positive	growth	30	mesophilic
43786	positive	optimum	37	mesophilic

Physiology and metabolism

Oxygen tolerance

43786

Oxygen tolerancefacultative anaerobe

Spore formation

@ref	Spore formation	Confidence
125439		96.4

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43786	NaCl	positive	growth	0-9 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43786	27613 ChEBI	amygdalin	-	fermentation
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68368	27613 ChEBI	amygdalin	+	fermentation	from API 20E
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
43786	16947 ChEBI	citrate	+	carbon source
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
43786	17634 ChEBI	D-glucose	+	fermentation
68368	17634 ChEBI	D-glucose	+	fermentation	from API 20E
68371	62318 ChEBI	D-lyxose	+	builds acid from	from API 50CH acid
43786	16899 ChEBI	D-mannitol	+	fermentation
68368	16899 ChEBI	D-mannitol	+	fermentation	from API 20E
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
43786	17924 ChEBI	D-sorbitol	+	fermentation
68371	17924 ChEBI	D-sorbitol	+	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	+	builds acid from	from API 50CH acid
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	+	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
43786	30849 ChEBI	L-arabinose	+	fermentation
68368	30849 ChEBI	L-arabinose	+	fermentation	from API 20E
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
43786	62345 ChEBI	L-rhamnose	+	fermentation
68368	62345 ChEBI	L-rhamnose	+	fermentation	from API 20E
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
43786	28053 ChEBI	melibiose	+	fermentation
68368	28053 ChEBI	melibiose	+	fermentation	from API 20E
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	+	builds acid from	from API 50CH acid
43786	43943 ChEBI	methyl alpha-D-mannoside	-	assimilation
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
43786	17268 ChEBI	myo-inositol	+	fermentation
68371	17268 ChEBI	myo-inositol	+	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
68368	17632 ChEBI	nitrate	-	reduction	from API 20E
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
43786	32032 ChEBI	potassium gluconate	-	assimilation
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68368	30911 ChEBI	sorbitol	+	fermentation	from API 20E
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
43786	17992 ChEBI	sucrose	+	fermentation
43786	27897 ChEBI	tryptophan	-	energy source
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	Group ID	Metabolite
43786	10	amoxicillin
43786		ampicillin
43786		cefalotin
43786		cefotaxime
43786		cefoxitin
43786		cefpodoxime
43786		ceftazidime
43786		cefuroxime
43786		ciprofloxacin
43786	10	clavulanic acid
43786		gentamicin
43786		imipenem
43786		meropenem
43786		nitrofurantoin
43786		norfloxacin
43786		piperacillin
43786		piperacillin + tazobactam
43786	26	sulfamethoxazole
43786		tetracycline
43786		tobramycin
43786	26	trimethoprim

Metabolite production

@ref	Chebi-ID	Metabolite
43786	15688 ChEBI	acetoin
68368	15688 ChEBI	acetoin	from API 20E
68368	17997 ChEBI	dinitrogen	from API 20E
43786	16136 ChEBI	hydrogen sulfide
68368	16136 ChEBI	hydrogen sulfide	from API 20E
43786	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E
68368	16301 ChEBI	nitrite	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
43786	15688 ChEBI	acetoin	-
68368	15688 ChEBI	acetoin	+	from API 20E
43786	35581 ChEBI	indole
68368	35581 ChEBI	indole		from API 20E

Enzymes

@ref	Value	Activity	Ec
43786	arginine dihydrolase	+	3.5.3.6
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
43786	beta-galactosidase	+	3.2.1.23
68368	beta-galactosidase	+	3.2.1.23	from API 20E
43786	catalase	+	1.11.1.6
43786	cytochrome oxidase	-	1.9.3.1
68368	cytochrome oxidase	-	1.9.3.1	from API 20E
43786	gelatinase	-
68368	gelatinase	-		from API 20E
43786	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
43786	ornithine decarboxylase	+	4.1.1.17
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
43786	tryptophan deaminase	-	4.1.99.1
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
43786	urease	-	3.5.1.5
68368	urease	-	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
24193	+	+	-	+	+	-	-	-	-	+	-	+	+	+/-	+	+	+/-	+	+	+	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
24193	+	+	-	+	+	-	-	-	-	+	-	+	+	+/-	+	+	+	+	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
24193	+	+	-	+	+	-	-	-	-	+	-	+	+	-	+	+	+	+	+	+	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
24193	-	+	-	-	+/-	+	+	-	-	-	+	+/-	+/-	+/-	-	+	+	+	+	+	-	+	-	-	+	+	+	+	+	+	+	+/-	+/-	-	-	+	-	-	-	+	-	+	-	-	-	-	-	-	-	-
24193	-	+	-	-	+/-	+	+	-	-	-	+	-	-	-	-	+/-	+	+	+/-	+	-	+	-	-	+	+	+	+	+	+	+/-	-	-	-	-	+/-	-	-	-	-	-	+	-	-	-	-	-	-	-	-
24193	-	+	-	-	+	+	+	-	-	-	+	+	+	+	+/-	+	+	+	+	+	-	+	-	-	+	+	+	+	+	+	+/-	+/-	+/-	-	-	+	-	-	-	+/-	-	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Human	#Child
#Host Body Product	#Fluids	#Blood

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
24193	human blood of a neonate	Mwanza	Tanzania	TZA	Africa
43786	A blood sample of neonate in Mwanza	Mwanza	Tanzania	TZA	Africa

Taxonmaps

69479

Global distribution of 16S sequence HQ122932 (>99% sequence identity) for Enterobacteriaceae from Microbeatlas

Aquatic Samples	1353
Soil Samples	615
Animal Samples	10132
Plant Samples	1234
Total Samples	13334

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24193	2	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	EB-247 assembly for Enterobacter bugandensis	complete	GCA_900324475	881260.9	2859686987	881260	99.3
124043	ASM1904690v1 assembly for Enterobacter bugandensis FDAARGOS 1427	complete	GCA_019046905	881260.84		881260	98.52
66792	E_bugandensis_EB-247T assembly for Enterobacter bugandensis	contig	GCA_900185975		2791355295	881260

Genome browser: GCA_900324475

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24193	Enterobacter bugandensis strain 247BMC 16S ribosomal RNA gene, partial sequence	HQ122932	1444		881260

GC content

@ref	GC-content (mol%)	Method
43786	56	genome sequence analysis
24193	56	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Enterobacter bugandensis EB-247
NCBI: GCA_900324475

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	96.40	no
125439	motility	BacteriaNetⓘ	yes	67.60	no
125439	gram_stain	BacteriaNetⓘ	negative	99.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	83.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Enterobacter bugandensis EB-247
NCBI: GCA_900324475.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.50	yes
125438	anaerobic	anaerobicⓘ	no	95.10	no
125438	aerobic	aerobicⓘ	no	64.81	yes
125438	spore-forming	spore-formingⓘ	no	88.30	no
125438	thermophilic	thermophileⓘ	no	99.80	yes
125438	flagellated	motile2+ⓘ	yes	80.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Generation of electrical energy in a microbial fuel cell coupling acetate oxidation to Fe(3+) reduction and isolation of the involved bacteria.	Becerril-Varela K, Serment-Guerrero JH, Manzanares-Leal GL, Ramirez-Duran N, Guerrero-Barajas C	World J Microbiol Biotechnol	10.1007/s11274-021-03077-4	2021
Metabolism	Enterobacter bugandensis: a novel enterobacterial species associated with severe clinical infection.	Pati NB, Doijad SP, Schultze T, Mannala GK, Yao Y, Jaiswal S, Ryan D, Suar M, Gwozdzinski K, Bunk B, Mraheil MA, Marahiel MA, Hegemann JD, Sproer C, Goesmann A, Falgenhauer L, Hain T, Imirzalioglu C, Mshana SE, Overmann J, Chakraborty T	Sci Rep	10.1038/s41598-018-23069-z	2018
Genetics	Multi-drug resistant Enterobacter bugandensis species isolated from the International Space Station and comparative genomic analyses with human pathogenic strains.	Singh NK, Bezdan D, Checinska Sielaff A, Wheeler K, Mason CE, Venkateswaran K	BMC Microbiol	10.1186/s12866-018-1325-2	2018
Phylogeny	Enterobacter bugandensis sp. nov., isolated from neonatal blood.	Doijad S, Imirzalioglu C, Yao Y, Pati NB, Falgenhauer L, Hain T, Foesel BU, Abt B, Overmann J, Mirambo MM, Mshana SE, Chakraborty T	Int J Syst Evol Microbiol	10.1099/ijsem.0.000821	2015

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24193	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29888
#43786	Swapnil Doijad, Can Imirzalioglu, Yancheng Yao, Niladri Bhusan Pati, Linda Falgenhauer, Torsten Hain, Bärbel U. Foesel, Birte Abt, Jö¨rg Overmann, Mariam M. Mirambo, Stephen E. Mshana, Trinad Chakraborty: Enterobacter bugandensis sp. nov., isolated from neonatal blood. IJSEM 66: 968 - 974 2016 ( DOI 10.1099/ijsem.0.000821 , PubMed 26637818 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68368	Automatically annotated from API 20E .
#68371	Automatically annotated from API 50CH acid .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Enterobacter bugandensis (DSM 29888, NCCB 100573, 247)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API 20E

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900324475

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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