Name: Brevibacillus gelatini PDF4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132387

Type strain:

Species: Brevibacillus gelatini

Strain Designation: PDF4

Culture col. no.: DSM 100115, NCCB 100559

Strain history: <- K. Inan, Karadeniz Techn. Univ., Trabzon, Turkey; PDF4

NCBI tax ID(s): 1655277 (species)

SI-ID 406986

DSM 100115

Section

Brevibacillus gelatini PDF4 is a facultative anaerobe, spore-forming, Gram-variable bacterium that forms circular colonies and was isolated from hot spring water.

colony-forming
Gram-variable
motile
rod-shaped
spore-forming
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Brevibacillus*
	Species	**Brevibacillus gelatini**
	Full Scientific Name (LPSN)	Brevibacillus gelatini Inan et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43776]	Gram stain	variable
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #43776]	Cell length	2-3.5 µm

[Ref.: #43776]	Cell width	0.8-1 µm

[Ref.: #43776]	Cell shape	rod-shaped

[Ref.: #43776]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %95.738

[Ref.: #43776]	Colony size	0.5-1 mm
[Ref.: #43776]	Colony color	Creamish-white
[Ref.: #43776]	Colony shape	circular
[Ref.: #43776]	Incubation period	3 days
[Ref.: #43776]	Cultivation medium used	Degryse 162 agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43776]

Culture medium

Degryse 162 agar

[Ref.: #43776]

Culture medium growth

[Ref.: #24176]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #24176]

Culture medium growth

[Ref.: #24176]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24176]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24176]	growth	40 °C
[Ref.: #43776]	growth	20-50 °C
[Ref.: #43776]	optimum	40 °C

[Ref.: #24176]	Temperature range	thermophilic
[Ref.: #43776]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #43776]			optimum	7
[Ref.: #43776]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43776]

Oxygen tolerance

facultative anaerobe

[Ref.: #43776]	Spore description	Ellipsoidal, occuring subterminally or centrally in swollen sporangia
[Ref.: #43776]	Type of spore	endospore
[Ref.: #43776]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43776]		NaCl		growth	0-2	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43776]	amygdalin ChEBI	-	builds acid from
[Ref.: #43776]	arbutin ChEBI	-	builds acid from
[Ref.: #43776]	casein	-	hydrolysis
[Ref.: #43776]	cellobiose ChEBI	-	builds acid from
[Ref.: #43776]	citrate ChEBI	-	assimilation
[Ref.: #43776]	cyclodextrin ChEBI	-	carbon source
[Ref.: #43776]	cyclodextrin ChEBI	-	energy source
[Ref.: #43776]	D-arabinose ChEBI	-	builds acid from
[Ref.: #43776]	D-arabitol ChEBI	-	builds acid from
[Ref.: #43776]	D-fructose ChEBI	-	builds acid from
[Ref.: #43776]	D-fucose ChEBI	-	builds acid from
[Ref.: #43776]	D-galactose ChEBI	-	builds acid from
[Ref.: #43776]	D-galactose ChEBI	-	carbon source
[Ref.: #43776]	D-galactose ChEBI	-	energy source
[Ref.: #43776]	D-glucose ChEBI	-	builds acid from
[Ref.: #43776]	D-glucose ChEBI	-	carbon source
[Ref.: #43776]	D-glucose ChEBI	-	energy source
[Ref.: #43776]	D-lyxose ChEBI	-	builds acid from
[Ref.: #43776]	D-mannitol ChEBI	-	builds acid from
[Ref.: #43776]	D-mannitol ChEBI	-	carbon source
[Ref.: #43776]	D-mannitol ChEBI	-	energy source
[Ref.: #43776]	D-mannose ChEBI	-	builds acid from
[Ref.: #43776]	D-mannose ChEBI	-	carbon source
[Ref.: #43776]	D-mannose ChEBI	-	energy source
[Ref.: #43776]	D-ribose ChEBI	-	builds acid from
[Ref.: #43776]	D-ribose ChEBI	-	carbon source
[Ref.: #43776]	D-ribose ChEBI	-	energy source
[Ref.: #43776]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #43776]	D-tagatose ChEBI	-	builds acid from
[Ref.: #43776]	D-tagatose ChEBI	-	carbon source
[Ref.: #43776]	D-tagatose ChEBI	-	energy source
[Ref.: #43776]	D-xylose ChEBI	-	builds acid from
[Ref.: #43776]	dithionitrobenzoic acid ChEBI	-	carbon source
[Ref.: #43776]	dithionitrobenzoic acid ChEBI	-	energy source
[Ref.: #43776]	erythritol ChEBI	-	builds acid from
[Ref.: #43776]	esculin ChEBI	-	hydrolysis
[Ref.: #43776]	esculin ferric citrate	-	builds acid from
[Ref.: #43776]	ferric citrate ChEBI	-	builds acid from
[Ref.: #43776]	galactitol ChEBI	-	builds acid from
[Ref.: #43776]	gelatin ChEBI	+	hydrolysis
[Ref.: #43776]	gentiobiose ChEBI	-	builds acid from
[Ref.: #43776]	glucose ChEBI	-	fermentation
[Ref.: #43776]	glycerol ChEBI	-	builds acid from
[Ref.: #43776]	glycogen ChEBI	-	builds acid from
[Ref.: #43776]	glycogen ChEBI	-	carbon source
[Ref.: #43776]	glycogen ChEBI	-	energy source
[Ref.: #43776]	inulin ChEBI	-	builds acid from
[Ref.: #43776]	inulin ChEBI	-	carbon source
[Ref.: #43776]	inulin ChEBI	-	energy source
[Ref.: #43776]	L-arabinose ChEBI	-	builds acid from
[Ref.: #43776]	L-arabitol ChEBI	-	builds acid from
[Ref.: #43776]	L-fucose ChEBI	-	builds acid from
[Ref.: #43776]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #43776]	L-rhamnose ChEBI	-	carbon source
[Ref.: #43776]	L-rhamnose ChEBI	-	energy source
[Ref.: #43776]	L-sorbose ChEBI	-	builds acid from
[Ref.: #43776]	L-xylose ChEBI	-	builds acid from
[Ref.: #43776]	lactose ChEBI	-	builds acid from
[Ref.: #43776]	maltose ChEBI	-	builds acid from
[Ref.: #43776]	maltotriose ChEBI	-	carbon source
[Ref.: #43776]	maltotriose ChEBI	-	energy source
[Ref.: #43776]	melezitose ChEBI	-	builds acid from
[Ref.: #43776]	melezitose ChEBI	-	carbon source
[Ref.: #43776]	melezitose ChEBI	-	energy source
[Ref.: #43776]	melibiose ChEBI	-	builds acid from
[Ref.: #43776]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #43776]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #43776]	methyl alpha-D-xylopyranoside	-	builds acid from
[Ref.: #43776]	methyl alpha-D-xylopyranoside	-	carbon source
[Ref.: #43776]	methyl alpha-D-xylopyranoside	-	energy source
[Ref.: #43776]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43776]	myo-inositol ChEBI	-	carbon source
[Ref.: #43776]	myo-inositol ChEBI	-	energy source
[Ref.: #43776]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #43776]	N-acetylglucosamine ChEBI	-	carbon source
[Ref.: #43776]	N-acetylglucosamine ChEBI	-	energy source
[Ref.: #43776]	nitrate ChEBI	-	reduction
[Ref.: #43776]	palatinose ChEBI	-	carbon source
[Ref.: #43776]	palatinose ChEBI	-	energy source
[Ref.: #43776]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #43776]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #43776]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #43776]	pyruvate ChEBI	-	carbon source
[Ref.: #43776]	pyruvate ChEBI	-	energy source
[Ref.: #43776]	raffinose ChEBI	-	builds acid from
[Ref.: #43776]	ribitol ChEBI	-	builds acid from
[Ref.: #43776]	salicin ChEBI	-	builds acid from
[Ref.: #43776]	starch ChEBI	-	builds acid from
[Ref.: #43776]	starch ChEBI	-	hydrolysis
[Ref.: #43776]	sucrose ChEBI	-	builds acid from
[Ref.: #43776]	trehalose ChEBI	-	builds acid from
[Ref.: #43776]	trehalose ChEBI	-	carbon source
[Ref.: #43776]	trehalose ChEBI	-	energy source
[Ref.: #43776]	turanose ChEBI	-	builds acid from
[Ref.: #43776]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43776]	tyrosine ChEBI	-	hydrolysis
[Ref.: #43776]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43776]		hydrogen sulfide ChEBI	no
[Ref.: #43776]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43776]	alanine arylamidase	+	3.4.11.2
[Ref.: #43776]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43776]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43776]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43776]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43776]	beta-galactosidase	-	3.2.1.23
[Ref.: #43776]	beta-glucosidase	-	3.2.1.21
[Ref.: #43776]	beta-mannosidase	-	3.2.1.25
[Ref.: #43776]	beta-xylosidase	-
[Ref.: #43776]	catalase	+	1.11.1.6
[Ref.: #43776]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43776]	glycin arylamidase	-
[Ref.: #43776]	L-aspartate arylamidase	-	3.4.11.21
[Ref.: #43776]	leucine arylamidase	+	3.4.11.1
[Ref.: #43776]	lysine decarboxylase	-	4.1.1.18
[Ref.: #43776]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43776]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #43776]	phenylalanine arylamidase	+
[Ref.: #43776]	proline-arylamidase	+	3.4.11.5
[Ref.: #43776]	pyrrolidonyl arylamidase	+	3.4.19.3
[Ref.: #43776]	tyrosine arylamidase	-
[Ref.: #43776]	urease	-	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24176]	Sample type/isolated from	hot spring water
[Ref.: #24176]	Geographic location (country and/or sea, region)	Aydin, Camkoy hot spring
[Ref.: #24176]	Country	Turkey
[Ref.: #24176]	Country ISO 3 Code	TUR
[Ref.: #24176]	Continent	Asia

[Ref.: #43776]	Sample type/isolated from	Water samples collected from the Camkoy hot spring; 150 m above sea level; Aydin coast
[Ref.: #43776]	Geographic location (country and/or sea, region)	Province of Aydin
[Ref.: #43776]	Country	Turkey
[Ref.: #43776]	Country ISO 3 Code	TUR
[Ref.: #43776]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Condition	#Thermophilic (>45°C)	-
#Environmental	#Aquatic	#Thermal spring

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KP899808 (>99% sequence identity) for Brevibacillus from Microbeatlas

Aquatic Samples	1150
Soil Samples	3695
Animal Samples	2257
Plant Samples	645
Total Samples	7747

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24176]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24176]	Brevibacillus gelatini strain PDF4 16S ribosomal RNA gene, partial sequence	KP899808	1354	ENA	1655277 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Brevibacillus gelatini DSM 100115	GCA_003710935	contig	GenBank	1655277 tax ID	*
[Ref.: #66792]	Brevibacillus gelatini strain DSM 100115	1655277.4	wgs	PATRIC	1655277 tax ID	*
[Ref.: #66792]	Brevibacillus gelatini DSM 100115	2839026242	draft	JGI IMG/M	1655277 tax ID	*

[Ref.: #24176]

GC-content

51.7 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Brevibacillus gelatini strain DSM 100115 PATRIC: 1655277.4
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Brevibacillus gelatini strain DSM 100115 PATRIC: 1655277.4
[Ref.: #69480]	motile	yes	90.648	yes
[Ref.: #69480]	gram-positive	yes	73.216	yes
[Ref.: #69480]	anaerobic	no	97.439	no
[Ref.: #69480]	aerobic	yes	74.113	yes
[Ref.: #69480]	halophile	no	94.494	no
[Ref.: #69480]	spore-forming	yes	97.393	yes
[Ref.: #69480]	thermophile	no	63.027	yes
[Ref.: #69480]	glucose-util	yes	89.383	no
[Ref.: #69480]	flagellated	yes	74.257	no
[Ref.: #69480]	glucose-ferment	no	92.158	yes

Availability in culture collections External links

[Ref.: #24176]

Culture collection no.

DSM 100115, NCCB 100559

[Ref.: #90730]

SI-ID 406986

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brevibacillus gelatini sp. nov., isolated from a hot spring.	Inan K, Ozer A, Ibrahim Guler H, Osman Belduz A, Canakci S	Int J Syst Evol Microbiol	10.1099/ijsem.0.000780	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24176

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100115

#43776

Kadriye Inan, Aysegul Ozer, Halil Ibrahim Guler, Ali Osman Belduz, Sabriye Canakci: Brevibacillus gelatini sp. nov., isolated from a hot spring. IJSEM 66: 712 - 718 2016 ( DOI 10.1099/ijsem.0.000780 , PubMed 26585900 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90730

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406986.1 )

* These data were automatically processed and therefore are not curated

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Brevibacillus gelatini strain DSM 100115

Brevibacillus gelatini strain DSM 100115

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