Brevibacillus gelatini (DSM 100115, NCCB 100559, PDF4)

Name: Brevibacillus gelatini (DSM 100115, NCCB 100559, PDF4)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132387

Type strain

Strain Designation PDF4

Culture col. no. DSM 100115 NCCB 100559 PDF4

NCBI tax ID(s) 1655277

Special Collections DSMZ Literature strains

History <- K. Inan, Karadeniz Techn. Univ., Trabzon, Turkey; PDF4

Links

Brevibacillus gelatini PDF4 is a facultative anaerobe, spore-forming, Gram-variable prokaryote that forms circular colonies and was isolated from hot spring water.

spore-forming Gram-variable motile rod-shaped colony-forming facultative anaerobe genome sequence 16S sequence

Name and taxonomic classification

SI-ID 406986

NCCB 100559,DSM 100115

@ref 20215

Last LPSN update 2026-02-09 11:31:42

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Paenibacillaceae

Genus Brevibacillus

Species Brevibacillus gelatini

Full scientific name Brevibacillus gelatini Inan et al. 2016

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
43776	variable	2-3.5 µm	0.8-1 µm	rod-shaped

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
43776	0.5-1 mm	Creamish-white	circular	3 days	Degryse 162 agar

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
43776	Degryse 162 agar
24176	CASO AGAR (MERCK 105458) (DSMZ Medium 220)		Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
24176	positive	growth	40	thermophilic
43776	positive	growth	20-50
43776	positive	optimum	40	thermophilic

Culture pH

@ref	Ability	Type	PH	PH range
43776	positive	optimum	7
43776	positive	growth	6-9	alkaliphile

Physiology and metabolism

Oxygen tolerance

43776

Oxygen tolerancefacultative anaerobe

Spore formation

@ref	Spore description	Type of spore	Spore formation	Confidence
43776	Ellipsoidal, occuring subterminally or centrally in swollen sporangia	endospore
125438				90.992

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43776	NaCl	positive	growth	0-2 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43776	27613 ChEBI	amygdalin	-	builds acid from
43776	18305 ChEBI	arbutin	-	builds acid from
43776		casein	-	hydrolysis
43776	17057 ChEBI	cellobiose	-	builds acid from
43776	16947 ChEBI	citrate	-	assimilation
43776	23456 ChEBI	cyclodextrin	-	carbon source
43776	23456 ChEBI	cyclodextrin	-	energy source
43776	17108 ChEBI	D-arabinose	-	builds acid from
43776	18333 ChEBI	D-arabitol	-	builds acid from
43776	15824 ChEBI	D-fructose	-	builds acid from
43776	28847 ChEBI	D-fucose	-	builds acid from
43776	12936 ChEBI	D-galactose	-	builds acid from
43776	12936 ChEBI	D-galactose	-	carbon source
43776	12936 ChEBI	D-galactose	-	energy source
43776	17634 ChEBI	D-glucose	-	builds acid from
43776	17634 ChEBI	D-glucose	-	carbon source
43776	17634 ChEBI	D-glucose	-	energy source
43776	62318 ChEBI	D-lyxose	-	builds acid from
43776	16899 ChEBI	D-mannitol	-	builds acid from
43776	16899 ChEBI	D-mannitol	-	carbon source
43776	16899 ChEBI	D-mannitol	-	energy source
43776	16024 ChEBI	D-mannose	-	builds acid from
43776	16024 ChEBI	D-mannose	-	carbon source
43776	16024 ChEBI	D-mannose	-	energy source
43776	16988 ChEBI	D-ribose	-	builds acid from
43776	16988 ChEBI	D-ribose	-	carbon source
43776	16988 ChEBI	D-ribose	-	energy source
43776	17924 ChEBI	D-sorbitol	-	builds acid from
43776	16443 ChEBI	D-tagatose	-	builds acid from
43776	16443 ChEBI	D-tagatose	-	carbon source
43776	16443 ChEBI	D-tagatose	-	energy source
43776	65327 ChEBI	D-xylose	-	builds acid from
43776	86228 ChEBI	dithionitrobenzoic acid	-	carbon source
43776	86228 ChEBI	dithionitrobenzoic acid	-	energy source
43776	17113 ChEBI	erythritol	-	builds acid from
43776	4853 ChEBI	esculin	-	hydrolysis
43776		esculin ferric citrate	-	builds acid from
43776	31605 ChEBI	ferric citrate	-	builds acid from
43776	16813 ChEBI	galactitol	-	builds acid from
43776	5291 ChEBI	gelatin	+	hydrolysis
43776	28066 ChEBI	gentiobiose	-	builds acid from
43776	17234 ChEBI	glucose	-	fermentation
43776	17754 ChEBI	glycerol	-	builds acid from
43776	28087 ChEBI	glycogen	-	builds acid from
43776	28087 ChEBI	glycogen	-	carbon source
43776	28087 ChEBI	glycogen	-	energy source
43776	15443 ChEBI	inulin	-	builds acid from
43776	15443 ChEBI	inulin	-	carbon source
43776	15443 ChEBI	inulin	-	energy source
43776	30849 ChEBI	L-arabinose	-	builds acid from
43776	18403 ChEBI	L-arabitol	-	builds acid from
43776	18287 ChEBI	L-fucose	-	builds acid from
43776	62345 ChEBI	L-rhamnose	-	builds acid from
43776	62345 ChEBI	L-rhamnose	-	carbon source
43776	62345 ChEBI	L-rhamnose	-	energy source
43776	17266 ChEBI	L-sorbose	-	builds acid from
43776	65328 ChEBI	L-xylose	-	builds acid from
43776	17716 ChEBI	lactose	-	builds acid from
43776	17306 ChEBI	maltose	-	builds acid from
43776	61993 ChEBI	maltotriose	-	carbon source
43776	61993 ChEBI	maltotriose	-	energy source
43776	6731 ChEBI	melezitose	-	builds acid from
43776	6731 ChEBI	melezitose	-	carbon source
43776	6731 ChEBI	melezitose	-	energy source
43776	28053 ChEBI	melibiose	-	builds acid from
43776	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from
43776	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from
43776		methyl alpha-D-xylopyranoside	-	builds acid from
43776		methyl alpha-D-xylopyranoside	-	carbon source
43776		methyl alpha-D-xylopyranoside	-	energy source
43776	17268 ChEBI	myo-inositol	-	builds acid from
43776	17268 ChEBI	myo-inositol	-	carbon source
43776	17268 ChEBI	myo-inositol	-	energy source
43776	506227 ChEBI	N-acetylglucosamine	-	builds acid from
43776	506227 ChEBI	N-acetylglucosamine	-	carbon source
43776	506227 ChEBI	N-acetylglucosamine	-	energy source
43776	17632 ChEBI	nitrate	-	reduction
43776	18394 ChEBI	palatinose	-	carbon source
43776	18394 ChEBI	palatinose	-	energy source
43776		potassium 2-dehydro-D-gluconate	-	builds acid from
43776		potassium 5-dehydro-D-gluconate	-	builds acid from
43776	32032 ChEBI	potassium gluconate	-	builds acid from
43776	15361 ChEBI	pyruvate	-	carbon source
43776	15361 ChEBI	pyruvate	-	energy source
43776	16634 ChEBI	raffinose	-	builds acid from
43776	15963 ChEBI	ribitol	-	builds acid from
43776	17814 ChEBI	salicin	-	builds acid from
43776	28017 ChEBI	starch	-	builds acid from
43776	28017 ChEBI	starch	-	hydrolysis
43776	17992 ChEBI	sucrose	-	builds acid from
43776	27082 ChEBI	trehalose	-	builds acid from
43776	27082 ChEBI	trehalose	-	carbon source
43776	27082 ChEBI	trehalose	-	energy source
43776	32528 ChEBI	turanose	-	builds acid from
43776	53426 ChEBI	tween 80	-	hydrolysis
43776	18186 ChEBI	tyrosine	-	hydrolysis
43776	17151 ChEBI	xylitol	-	builds acid from

Metabolite production

@ref	Chebi-ID	Metabolite	Production
43776	16136 ChEBI	hydrogen sulfide
43776	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
43776	alanine arylamidase	+	3.4.11.2
43776	alpha-galactosidase	-	3.2.1.22
43776	alpha-glucosidase	-	3.2.1.20
43776	alpha-mannosidase	-	3.2.1.24
43776	arginine dihydrolase	-	3.5.3.6
43776	beta-galactosidase	-	3.2.1.23
43776	beta-glucosidase	-	3.2.1.21
43776	beta-mannosidase	-	3.2.1.25
43776	beta-xylosidase	-
43776	catalase	+	1.11.1.6
43776	cytochrome oxidase	+	1.9.3.1
43776	glycin arylamidase	-
43776	L-aspartate arylamidase	-	3.4.11.21
43776	leucine arylamidase	+	3.4.11.1
43776	lysine decarboxylase	-	4.1.1.18
43776	N-acetyl-beta-glucosaminidase	-	3.2.1.52
43776	ornithine decarboxylase	-	4.1.1.17
43776	phenylalanine arylamidase	+
43776	proline-arylamidase	+	3.4.11.5
43776	pyrrolidonyl arylamidase	+	3.4.19.3
43776	tyrosine arylamidase	-
43776	urease	-	3.5.1.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Condition	#Thermophilic (>45°C)	-
#Environmental	#Aquatic	#Thermal spring

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
24176	hot spring water	Aydin, Camkoy hot spring	Turkey	TUR	Asia
43776	Water samples collected from the Camkoy hot spring; 150 m above sea level; Aydin coast	Province of Aydin	Turkey	TUR	Asia

Taxonmaps

69479

Global distribution of 16S sequence KP899808 (>99% sequence identity) for Brevibacillus from Microbeatlas

Aquatic Samples	1150
Soil Samples	3695
Animal Samples	2257
Plant Samples	645
Total Samples	7747

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24176	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM371093v1 assembly for Brevibacillus gelatini DSM 100115	contig	GCA_003710935	1655277.4	2839026242	1655277	55.54

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24176	Brevibacillus gelatini strain PDF4 16S ribosomal RNA gene, partial sequence	KP899808	1354		1655277

GC content

@ref	GC-content (mol%)	Method
24176	51.7	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Brevibacillus gelatini DSM 100115
NCBI: GCA_003710935

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	82.70	no
125439	motility	BacteriaNetⓘ	yes	83.70	no
125439	gram_stain	BacteriaNetⓘ	variable	85.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	77.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Brevibacillus gelatini strain DSM 100115
PATRIC: 1655277.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	54.70	yes
125438	anaerobic	anaerobicⓘ	no	95.54	yes
125438	spore-forming	spore-formingⓘ	yes	90.99	no
125438	aerobic	aerobicⓘ	yes	76.44	no
125438	thermophilic	thermophileⓘ	no	83.33	no
125438	flagellated	motile2+ⓘ	yes	81.30	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Evolution-Guided Discovery of Antimycobacterial Triculamin-Like Lasso Peptides.	Merrild A, Svenningsen T, Chevrette MG, Torring T.	Angew Chem Int Ed Engl	10.1002/anie.202425134	2025
Phylogeny	Brevibacillus gelatini sp. nov., isolated from a hot spring.	Inan K, Ozer A, Ibrahim Guler H, Osman Belduz A, Canakci S	Int J Syst Evol Microbiol	10.1099/ijsem.0.000780	2015

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24176	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100115
#43776	Kadriye Inan, Aysegul Ozer, Halil Ibrahim Guler, Ali Osman Belduz, Sabriye Canakci: Brevibacillus gelatini sp. nov., isolated from a hot spring. IJSEM 66: 712 - 718 2016 ( DOI 10.1099/ijsem.0.000780 , PubMed 26585900 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Brevibacillus gelatini (DSM 100115, NCCB 100559, PDF4)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003710935

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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