Name: Haloimpatiens lingqiaonensis ZC-CMC3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132386

Type strain:

Species: Haloimpatiens lingqiaonensis

Strain Designation: ZC-CMC3

Culture col. no.: DSM 103556, CCTCC AB 2013104, JCM 19210, KCTC 15321

Strain history: <- Nai-Fang Zhang, Zhejiang Univ <- Tian-Tian Li

NCBI tax ID(s): 1380675 (species)

SI-ID 407780

DSM 103556

Section

Haloimpatiens lingqiaonensis ZC-CMC3 is an anaerobe, chemoorganotroph, Gram-positive bacterium that forms circular colonies and was isolated from wastewater of a paper mill.

colony-forming
Gram-positive
motile
rod-shaped
anaerobe
chemoorganotroph
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Haloimpatiens*
	Species	**Haloimpatiens lingqiaonensis**
	Full Scientific Name (LPSN)	Haloimpatiens lingqiaonensis Wu et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43790]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #43790]	Cell length	2.9-5.1 µm

[Ref.: #43790]	Cell width	0.6-1.2 µm

[Ref.: #43790]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #43790]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #43790]	Flagellum arrangement	peritrichous
[Ref.: #67771]	Flagellum arrangement	peritrichous

[Ref.: #43790]	Colony size	0.5-1 mm
[Ref.: #43790]	Colony color	White
[Ref.: #43790]	Colony shape	circular
[Ref.: #43790]	Incubation period	2-3 days
[Ref.: #43790]	Cultivation medium used	Gs agar-shake

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43790]

Culture medium

Gs agar-shake

[Ref.: #43790]

Culture medium growth

[Ref.: #24175]

Culture medium

CLOSTRIDIUM (GS) MEDIUM (DSMZ Medium 255)

[Ref.: #24175]

Culture medium growth

[Ref.: #24175]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24175]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24175]	growth	37 °C
[Ref.: #43790]	growth	25-48 °C
[Ref.: #43790]	optimum	43 °C
[Ref.: #67770]	growth	37 °C
[Ref.: #67771]	growth	37 °C

[Ref.: #24175]	Temperature range	mesophilic
[Ref.: #43790]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43790]			optimum	7
[Ref.: #43790]			growth	5.5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43790]	Oxygen tolerance	anaerobe
[Ref.: #67771]	Oxygen tolerance	anaerobe

[Ref.: #43790]

Nutrition type

chemoorganotroph

[Ref.: #43790]	Ability of spore formation	no
[Ref.: #67771]	Ability of spore formation	no

[Ref.: #43790]

Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43790]		NaCl		growth	0-3	%(w/v)
[Ref.: #43790]		NaCl		optimum	0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43790]	acetate ChEBI	-	growth
[Ref.: #43790]	arabinose ChEBI	-	growth
[Ref.: #43790]	beef extract	+	growth
[Ref.: #43790]	butyrate ChEBI	-	growth
[Ref.: #43790]	carboxymethylcellulose ChEBI	-	growth
[Ref.: #43790]	cellobiose ChEBI	-	growth
[Ref.: #43790]	chitosan ChEBI	-	growth
[Ref.: #43790]	dl-alanine ChEBI	-	growth
[Ref.: #43790]	elemental sulfur ChEBI	-	electron acceptor
[Ref.: #43790]	formate ChEBI	-	growth
[Ref.: #43790]	fructose ChEBI	-	growth
[Ref.: #43790]	fumarate ChEBI	-	growth
[Ref.: #43790]	glucose ChEBI	-	growth
[Ref.: #43790]	glycerol ChEBI	-	growth
[Ref.: #43790]	glycine ChEBI	-	growth
[Ref.: #43790]	L-arginine ChEBI	-	growth
[Ref.: #43790]	L-proline ChEBI	-	growth
[Ref.: #43790]	L-valine ChEBI	-	growth
[Ref.: #43790]	maltose ChEBI	-	growth
[Ref.: #43790]	peptone	+	growth
[Ref.: #43790]	pyruvate ChEBI	-	growth
[Ref.: #43790]	sodium nitrate ChEBI	-	electron acceptor
[Ref.: #43790]	sodium nitrite ChEBI	-	electron acceptor
[Ref.: #43790]	sodium sulfate ChEBI	-	electron acceptor
[Ref.: #43790]	sodium sulfite ChEBI	-	electron acceptor
[Ref.: #43790]	sodium thiosulfate ChEBI	-	electron acceptor
[Ref.: #43790]	sucrose ChEBI	-	growth
[Ref.: #43790]	tryptone	+	growth
[Ref.: #43790]	xylose ChEBI	-	growth
[Ref.: #43790]	yeast extract	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43790]		catalase	-	1.11.1.6
[Ref.: #43790]		cytochrome oxidase	-	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24175]	Sample type/isolated from	wastewater of a paper mill
[Ref.: #24175]	Geographic location (country and/or sea, region)	Zhejiang
[Ref.: #24175]	Country	China
[Ref.: #24175]	Country ISO 3 Code	CHN
[Ref.: #24175]	Continent	Asia
[Ref.: #24175]	Geographic location	30.0331°/120.0190°

[Ref.: #43790]	Sample type/isolated from	A wastewater sample collected from a paper mill in Lingqiao town
[Ref.: #43790]	Geographic location (country and/or sea, region)	Lingqiao town, Zhejiang
[Ref.: #43790]	Country	China
[Ref.: #43790]	Country ISO 3 Code	CHN
[Ref.: #43790]	Continent	Asia
[Ref.: #43790]	Geographic location	30.0330°/120.0190°

[Ref.: #67770]	Sample type/isolated from	Wastewater of a paper mill in Lingqiao Town
[Ref.: #67770]	Geographic location (country and/or sea, region)	Zhejiang
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From wastewater of a paper mill factory
[Ref.: #67771]	Geographic location (country and/or sea, region)	Lingqiao town, Zhejiang
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Plant (Factory)
#Engineered	#Built environment	-
#Engineered	#Waste	#Wastewater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KC869664 (>99% sequence identity) for Haloimpatiens lingqiaonensis from Microbeatlas

Aquatic Samples	373
Soil Samples	251
Animal Samples	529
Plant Samples	29
Total Samples	1182

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24175]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24175]	Haloimpatiens lingqiaonensis strain ZC-CMC3 16S ribosomal RNA gene, partial sequence	KC869664	1476	ENA	1380675 tax ID

[Ref.: #24175]	GC-content	30 mol%
[Ref.: #67770]	GC-content	30 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	30.0 mol%

Availability in culture collections External links

[Ref.: #24175]

Culture collection no.

DSM 103556, CCTCC AB 2013104, JCM 19210, KCTC 15321

[Ref.: #90729]

SI-ID 407780

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Haloimpatiens lingqiaonensis gen. nov., sp. nov., an anaerobic bacterium isolated from paper-mill wastewater.	Wu D, Zhang NF, Sun C, Zhang WW, Han SB, Pan J, Wu M, Th D, Zhu XF	Int J Syst Evol Microbiol	10.1099/ijsem.0.000765	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24175

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103556

#43790

Dildar. Wu, Nai-Fang Zhang, Cong Sun, Wen-Wu Zhang, Shuai-Bo Han, Jie Pan, Min Wu, Dilbar. Th, Xu-Fen Zhu: Haloimpatiens lingqiaonensis gen. nov., sp. nov., an anaerobic bacterium isolated from paper-mill wastewater. IJSEM 66: 628 - 632 2016 ( DOI 10.1099/ijsem.0.000765 , PubMed 26559355 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#90729

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407780.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Haloimpatiens lingqiaonensis in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF