Name: Bradyrhizobium vignae 7-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132365

Type strain:

Species: Bradyrhizobium vignae

Strain Designation: 7-2

Culture col. no.: DSM 100297, LMG 28791, NTCCM 0018, DSMZ 100297

Strain history: <- B. Reinhold-Hurek, Univ. Bremen, Germany; 7-2 <- J. L. Grönemeyer

NCBI tax ID(s): 1549949 (species)

SI-ID 396451

LMG 28791, DSM 100297

Section

Bradyrhizobium vignae 7-2 is an aerobe, thermophilic, Gram-negative bacterium that forms circular colonies and was isolated from effective nodule of Vigna unguiculata .

colony-forming
Gram-negative
motile
rod-shaped
aerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Nitrobacteraceae*
	Genus	*Bradyrhizobium*
	Species	**Bradyrhizobium vignae**
	Full Scientific Name (LPSN)	Bradyrhizobium vignae Grönemeyer et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #44038]	Gram stain	negative

[Ref.: #44038]	Cell length	2-3 µm

[Ref.: #44038]	Cell width	0.7 µm

[Ref.: #44038]	Cell shape	rod-shaped

[Ref.: #44038]	Motility	yes

[Ref.: #44038]	Colony size	0.2-1 mm
[Ref.: #44038]	Colony color	beige-whitish
[Ref.: #44038]	Colony shape	circular
[Ref.: #44038]	Incubation period	8 days
[Ref.: #44038]	Cultivation medium used	YMA

Information on culture and growth conditions Culture and growth conditions

[Ref.: #44038]

Culture medium

YMA

[Ref.: #44038]

Culture medium growth

[Ref.: #24154]

Culture medium

RHIZOBIUM MEDIUM (DSMZ Medium 98)

[Ref.: #24154]

Culture medium growth

[Ref.: #24154]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24154]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24154]	growth	28 °C
[Ref.: #44038]	growth	40 °C
[Ref.: #44038]	growth	38 °C
[Ref.: #44038]	growth	37 °C
[Ref.: #44038]	growth	28 °C

[Ref.: #24154]	Temperature range	mesophilic
[Ref.: #44038]	Temperature range	thermophilic
[Ref.: #44038]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #44038]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44038]

Oxygen tolerance

aerobe

[Ref.: #44038]	Type of spore	spore
[Ref.: #44038]	Ability of spore formation	no

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #44038]		NaCl	no	growth	1	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #44038]	2,3-butanediol ChEBI	-	oxidation
[Ref.: #44038]	2-oxoglutarate ChEBI	+	oxidation
[Ref.: #44038]	2-oxopentanoate ChEBI	+	oxidation
[Ref.: #44038]	4-hydroxy-L-proline ChEBI	-	oxidation
[Ref.: #44038]	4-hydroxybutyrate ChEBI	+	oxidation
[Ref.: #44038]	alpha-cyclodextrin ChEBI	-	oxidation
[Ref.: #44038]	alpha-D-glucose ChEBI	+	oxidation
[Ref.: #44038]	alpha-lactose ChEBI	-	oxidation
[Ref.: #44038]	beta-hydroxybutyrate ChEBI	+	oxidation
[Ref.: #44038]	bromosuccinate ChEBI	+	oxidation
[Ref.: #44038]	cellobiose ChEBI	-	oxidation
[Ref.: #44038]	cis-aconitate ChEBI	+	oxidation
[Ref.: #44038]	citrate ChEBI	+	oxidation
[Ref.: #44038]	D-arabitol ChEBI	+	oxidation
[Ref.: #44038]	D-galactonic acid lactone ChEBI	+	oxidation
[Ref.: #44038]	D-galactose ChEBI	+	oxidation
[Ref.: #44038]	D-galacturonic acid ChEBI	+	oxidation
[Ref.: #44038]	D-gluconate ChEBI	+	oxidation
[Ref.: #44038]	D-glucosaminic acid ChEBI	+	oxidation
[Ref.: #44038]	D-glucose 6-phosphate ChEBI	-	oxidation
[Ref.: #44038]	D-mannitol ChEBI	+	oxidation
[Ref.: #44038]	D-mannose ChEBI	+	oxidation
[Ref.: #44038]	D-psicose ChEBI	-	oxidation
[Ref.: #44038]	D-saccharate ChEBI	+	oxidation
[Ref.: #44038]	D-serine ChEBI	-	oxidation
[Ref.: #44038]	D-sorbitol ChEBI	-	oxidation
[Ref.: #44038]	DL-carnitine ChEBI	-	oxidation
[Ref.: #44038]	erythritol ChEBI	-	oxidation
[Ref.: #44038]	ethanolamine ChEBI	-	oxidation
[Ref.: #44038]	formate ChEBI	+	oxidation
[Ref.: #44038]	gamma-aminobutyric acid ChEBI	-	oxidation
[Ref.: #44038]	gentiobiose ChEBI	-	oxidation
[Ref.: #44038]	glucose 1-phosphate ChEBI	-	oxidation
[Ref.: #44038]	glucuronamide ChEBI	+	oxidation
[Ref.: #44038]	glycerol ChEBI	+	oxidation
[Ref.: #44038]	glycerol 1-phosphate ChEBI	-	oxidation
[Ref.: #44038]	glycogen ChEBI	-	oxidation
[Ref.: #44038]	inosine ChEBI	-	oxidation
[Ref.: #44038]	L-alaninamide ChEBI	-	oxidation
[Ref.: #44038]	L-alanylglycine ChEBI	-	oxidation
[Ref.: #44038]	L-arabinose ChEBI	+	oxidation
[Ref.: #44038]	L-asparagine ChEBI	-	oxidation
[Ref.: #44038]	L-aspartate ChEBI	+	oxidation
[Ref.: #44038]	L-fructose ChEBI	+	oxidation
[Ref.: #44038]	L-glutamate ChEBI	+	oxidation
[Ref.: #44038]	L-histidine ChEBI	-	oxidation
[Ref.: #44038]	L-leucine ChEBI	+	oxidation
[Ref.: #44038]	L-ornithine ChEBI	-	oxidation
[Ref.: #44038]	L-proline ChEBI	-	oxidation
[Ref.: #44038]	L-pyroglutamic acid ChEBI	+	oxidation
[Ref.: #44038]	L-serine ChEBI	-	oxidation
[Ref.: #44038]	L-threonine ChEBI	-	oxidation
[Ref.: #44038]	lactate ChEBI	+	oxidation
[Ref.: #44038]	lactulose ChEBI	-	oxidation
[Ref.: #44038]	malonate ChEBI	+	oxidation
[Ref.: #44038]	maltose ChEBI	-	oxidation
[Ref.: #44038]	melibiose ChEBI	-	oxidation
[Ref.: #44038]	methyl beta-D-glucopyranoside ChEBI	-	oxidation
[Ref.: #44038]	methyl pyruvate ChEBI	+	oxidation
[Ref.: #44038]	monomethyl succinate ChEBI	+	oxidation
[Ref.: #44038]	myo-inositol ChEBI	-	oxidation
[Ref.: #44038]	N-acetylgalactosamine ChEBI	-	oxidation
[Ref.: #44038]	N-acetylglucosamine ChEBI	-	oxidation
[Ref.: #44038]	phenylethylamine ChEBI	-	oxidation
[Ref.: #44038]	propionate ChEBI	+	oxidation
[Ref.: #44038]	putrescine ChEBI	-	oxidation
[Ref.: #44038]	quinate ChEBI	+	oxidation
[Ref.: #44038]	raffinose ChEBI	-	oxidation
[Ref.: #44038]	ribitol ChEBI	-	oxidation
[Ref.: #44038]	sebacic acid ChEBI	+	oxidation
[Ref.: #44038]	succinamate ChEBI	+	oxidation
[Ref.: #44038]	succinate ChEBI	+	oxidation
[Ref.: #44038]	sucrose ChEBI	-	oxidation
[Ref.: #44038]	thymidine ChEBI	-	oxidation
[Ref.: #44038]	trehalose ChEBI	-	oxidation
[Ref.: #44038]	turanose ChEBI	-	oxidation
[Ref.: #44038]	tween 40 ChEBI	+	oxidation
[Ref.: #44038]	tween 80 ChEBI	+	oxidation
[Ref.: #44038]	uridine ChEBI	-	oxidation
[Ref.: #44038]	urocanic acid ChEBI	+	oxidation
[Ref.: #44038]	xylitol ChEBI	-	oxidation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic resistance	Concentration
[Ref.: #44038]	chloramphenicol ChEBI	yes	50	µg/mL
[Ref.: #44038]	streptomycin ChEBI	yes	5	µg/mL
[Ref.: #44038]	tetracycline ChEBI	yes	15	µg/mL

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24154]	Sample type/isolated from	effective nodule of Vigna unguiculata (cowpea)
[Ref.: #24154]	Host species	Vigna unguiculata
[Ref.: #24154]	Geographic location (country and/or sea, region)	Kavango region, near Mashare
[Ref.: #24154]	Country	Namibia
[Ref.: #24154]	Country ISO 3 Code	NAM
[Ref.: #24154]	Continent	Africa
[Ref.: #24154]	Geographic location	-17.8928°/20.2105°

[Ref.: #44038]	Sample type/isolated from	effective nodule of Vigna unguiculata (cowpea)
[Ref.: #44038]	Geographic location (country and/or sea, region)	Kavango Region
[Ref.: #44038]	Country	Namibia
[Ref.: #44038]	Country ISO 3 Code	NAM
[Ref.: #44038]	Continent	Africa
[Ref.: #44038]	Enrichment culture	MAG medium (van Berkum, 1990)
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KP899563 (>99% sequence identity) for Bradyrhizobium from Microbeatlas

Aquatic Samples	2827
Soil Samples	5001
Animal Samples	4320
Plant Samples	739
Total Samples	12887

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24154]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24154]	Bradyrhizobium vignae strain 7-2 16S ribosomal RNA gene, partial sequence	KP899563	1298	ENA	1549949 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bradyrhizobium vignae LMG 28791	GCA_004114425	scaffold	GenBank	1549949 tax ID	*
[Ref.: #66792]	Bradyrhizobium vignae strain LMG 28791	1549949.9	wgs	PATRIC	1549949 tax ID	*
[Ref.: #66792]	Bradyrhizobium vignae LMG 28791	2881665667	draft	JGI IMG/M	1549949 tax ID	*

[Ref.: #44038]	GC-content	65.4 mol%	high performance liquid chromatography (HPLC)
[Ref.: #24154]	GC-content	65.4 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bradyrhizobium vignae LMG 28791 NCBI: GCA_004114425.1
[Ref.: #69480]	motile	yes	81.391	no
[Ref.: #69480]	flagellated	no	78.163	no
[Ref.: #69480]	gram-positive	no	97.943	no
[Ref.: #69480]	anaerobic	no	96.902	yes
[Ref.: #69480]	aerobic	yes	91.839	yes
[Ref.: #69480]	halophile	no	94.775	no
[Ref.: #69480]	spore-forming	no	93.157	no
[Ref.: #69480]	thermophile	no	99.327	no
[Ref.: #69480]	glucose-util	yes	90.103	no
[Ref.: #69480]	glucose-ferment	no	89.729	no

Availability in culture collections External links

[Ref.: #24154]

Culture collection no.

DSM 100297, LMG 28791, NTCCM 0018, DSMZ 100297

[Ref.: #90711]

SI-ID 396451

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bradyrhizobium vignae sp. nov., a nitrogen-fixing symbiont isolated from effective nodules of Vigna and Arachis.	Gronemeyer JL, Hurek T, Bunger W, Reinhold-Hurek B	Int J Syst Evol Microbiol	10.1099/ijsem.0.000674	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24154

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100297

#44038

Jann Lasse Grönemeyer, Thomas Hurek, Wiebke Bünger and Barbara Reinhold-Hurek: Bradyrhizobium vignae sp. nov., a nitrogen-fixing symbiont isolated from effective nodules of Vigna and Arachis. IJSEM 66: 62 - 69 2016 ( DOI 10.1099/ijsem.0.000674 , PubMed 26463703 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#90711

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID396451.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Bradyrhizobium vignae in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Bradyrhizobium vignae LMG 28791

Change proposal

Change proposal