Streptomyces alfalfae (DSM 103384, CCTCC AA 2015019, KCTC 39571)

Name: Streptomyces alfalfae (DSM 103384, CCTCC AA 2015019, KCTC 39571)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132341

Type strain

Strain Designation XY25

Culture col. no. DSM 103384 CCTCC AA 2015019 KCTC 39571 XY25

NCBI tax ID(s) 1642299

Special Collections DSMZ KCTC Literature strains

History DSMZ <- CCTCC; CCTCC AA2015019

Links

Streptomyces alfalfae XY25 is an aerobe, spore-forming, Gram-positive bacterium that builds branched substrate mycelia and aerial and was isolated from rhizosphere soil.

spore-forming Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 407969

DSM 103384

@ref 20215

Last LPSN update 2026-05-29 08:37:50

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Kitasatosporales

Family Streptomycetaceae

Genus Streptomyces

Species Streptomyces alfalfae

Full scientific name Streptomyces alfalfae She et al. 2016

Morphology

Cell morphology

@ref	Gram stain	Confidence
65213	positive
125439	positive	99.497
125439		94.629
125438	positive	90.8

Colony morphology

@ref	Colony color	Medium used
65213	light brown	yeast extract-malt extract agar

Multicellular morphology

@ref	Forms multicellular complex	Complex name	Complex color
65213		branched substrate mycelia and aerial	white in most media

Multimedia

@ref:	24130
multimedia content:	DSM_103384.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_103384.jpg
caption:	Medium 65 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
24130	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
65213	tryptone-yeast extract broth
65213	yeast extract-malt extract agar
65213	oatmeal agar
65213	inorganic salts-starch agar
65213	tyrosine agar
65213	glycerol-asparagine agar
65213	peptone-yeast extract agar
24130	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)	Medium recipe at MediaDive	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
24130	STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)	Medium recipe at MediaDive	Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 20.0 g/l Starch 10.0 g/l (NH4)2SO4 2.0 g/l CaCO3 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l NaCl 1.0 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
24130	positive	growth	28
65213	positive	growth	16-45
65213	positive	optimum	37

Culture pH

@ref	Ability	Type	PH	PH range
65213	positive	growth	6-10	alkaliphile
65213		growth	5

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
65213	aerobe
125438	aerobe	90.264
125439	obligate aerobe	97.874

Spore formation

@ref	Spore description	Type of spore	Spore formation	Confidence
65213	long flexuous spore chains with a smooth surface and long rod-shaped spores	spore
125438				90.988

Halophily

@ref	Salt	Growth	Tested relation	Concentration
65213	NaCl	positive	growth	0-6 %
65213	NaCl		growth	8 %(w/v)

Observation

65213

ObservationThe major menaquinones are MK-9(H 6 ) and MK-9(H 8 )

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
65213	16708 ChEBI	adenine	+	degradation
65213		casein	+	degradation
65213	17057 ChEBI	cellobiose	-	carbon source
65213	17108 ChEBI	D-arabinose	+	carbon source
65213	17108 ChEBI	D-arabinose	+	energy source
65213	15824 ChEBI	D-fructose	+	carbon source
65213	15824 ChEBI	D-fructose	+	energy source
65213	12936 ChEBI	D-galactose	+	builds acid from
65213	12936 ChEBI	D-galactose	+	carbon source
65213	12936 ChEBI	D-galactose	+	energy source
65213	17634 ChEBI	D-glucose	+	builds acid from
65213	17634 ChEBI	D-glucose	+	carbon source
65213	17634 ChEBI	D-glucose	+	energy source
65213	16899 ChEBI	D-mannitol	+	carbon source
65213	16899 ChEBI	D-mannitol	+	energy source
65213	16024 ChEBI	D-mannose	+	builds acid from
65213	16024 ChEBI	D-mannose	+	carbon source
65213	16024 ChEBI	D-mannose	+	energy source
65213	16988 ChEBI	D-ribose	+	builds acid from
65213	16988 ChEBI	D-ribose	+	carbon source
65213	16988 ChEBI	D-ribose	+	energy source
65213	17924 ChEBI	D-sorbitol	+	carbon source
65213	17924 ChEBI	D-sorbitol	+	energy source
65213	65327 ChEBI	D-xylose	+	carbon source
65213	65327 ChEBI	D-xylose	+	energy source
65213	17113 ChEBI	erythritol	+	carbon source
65213	17113 ChEBI	erythritol	+	energy source
65213	4853 ChEBI	esculin	+	degradation
65213	28757 ChEBI	fructose	+	builds acid from
65213	5291 ChEBI	gelatin	+	carbon source
65213	5291 ChEBI	gelatin	+	energy source
65213	5291 ChEBI	gelatin	+	hydrolysis
65213	17754 ChEBI	glycerol	+	builds acid from
65213	17754 ChEBI	glycerol	+	carbon source
65213	17754 ChEBI	glycerol	+	energy source
65213	17368 ChEBI	hypoxanthine	+	degradation
65213	17196 ChEBI	L-asparagine	+	nitrogen source
65213	29991 ChEBI	L-aspartate	+	nitrogen source
65213	29985 ChEBI	L-glutamate	+	nitrogen source
65213	15971 ChEBI	L-histidine	+	nitrogen source
65213	62345 ChEBI	L-rhamnose	+	carbon source
65213	62345 ChEBI	L-rhamnose	+	energy source
65213	16857 ChEBI	L-threonine	+	nitrogen source
65213	17895 ChEBI	L-tyrosine	+	degradation
65213	17716 ChEBI	lactose	+	carbon source
65213	17716 ChEBI	lactose	+	energy source
65213	17306 ChEBI	maltose	+	carbon source
65213	17306 ChEBI	maltose	+	energy source
65213	28053 ChEBI	melibiose	-	carbon source
65213	16811 ChEBI	methionine	-	nitrogen source
65213	17268 ChEBI	myo-inositol	-	carbon source
65213	506227 ChEBI	N-acetylglucosamine	+	builds acid from
65213	506227 ChEBI	N-acetylglucosamine	+	carbon source
65213	506227 ChEBI	N-acetylglucosamine	+	energy source
65213	16634 ChEBI	raffinose	+	carbon source
65213	16634 ChEBI	raffinose	+	energy source
65213	17814 ChEBI	salicin	+	carbon source
65213	17814 ChEBI	salicin	+	energy source
65213	28017 ChEBI	starch	-	hydrolysis
65213	28017 ChEBI	starch	+	carbon source
65213	28017 ChEBI	starch	+	energy source
65213	17992 ChEBI	sucrose	+	carbon source
65213	17992 ChEBI	sucrose	+	energy source
65213	27082 ChEBI	trehalose	+	carbon source
65213	27082 ChEBI	trehalose	+	energy source
65213	15318 ChEBI	xanthine	+	degradation
65213	17151 ChEBI	xylitol	+	carbon source
65213	17151 ChEBI	xylitol	+	energy source

Fatty acid profile

Metadata FA analysis

@ref

65213

Fatty acid	Percentage
C15:0 anteiso	31.5
C16:0 iso	15.4
C17:0 anteiso	14.1
C17:1 anteiso ω9c	8.1
C16:0	6.3
iso-C15:0 2-OH and C16:1ω6c and C16:1ω	6.2
C15:0 iso	2.9
C18:0	0.2

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Host Body-Site	#Plant	#Rhizosphere
#Host	#Plants	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
24130	rhizosphere soil	Shanxi province, Jingyang, alfalfa field	China	CHN	Asia
65213	rhizosphere soil	alfalfa field in Jingyang, Shanxi province	China	CHN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24130	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM412744v1 assembly for Streptomyces alfalfae XY25	contig	GCA_004127445	1642299.4	8110224887	1642299	65.75
66792	ASM421244v1 assembly for Streptomyces alfalfae XY25	contig	GCA_004212445	1642299.5	8110970144	1642299	30.92

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24130	Streptomyces alfalfae 16S ribosomal RNA gene, partial sequence	KR080524	1487		1642299

GC content

@ref	GC-content (mol%)	Method
24130	71.8	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM421244v1 assembly for Streptomyces alfalfae XY25
NCBI: GCA_004212445

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.87	no
125439	motility	BacteriaNetⓘ	no	94.63	no
125439	gram_stain	BacteriaNetⓘ	positive	99.50	no
125439	spore_formation	BacteriaNetⓘ	yes	87.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Streptomyces alfalfae XY25
NCBI: GCA_004127445.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.80	yes
125438	anaerobic	anaerobicⓘ	no	98.39	yes
125438	aerobic	aerobicⓘ	yes	90.26	yes
125438	spore-forming	spore-formingⓘ	yes	90.99	yes
125438	thermophilic	thermophileⓘ	no	94.50	yes
125438	flagellated	motile2+ⓘ	no	89.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Streptomyces alfalfae sp. nov. and comparisons with its closest taxa Streptomyces silaceus, Streptomyces flavofungini and Streptomyces intermedius.	She W, Sun Z, Yi L, Zhao S, Liang Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.000671	2015

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24130	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103384
#65213	Wenqing She, Zhongfeng Sun, Lei Yi, Shumiao Zhao, Yunxiang Liang: Streptomyces alfalfae sp. nov. and comparisons with its closest taxa Streptomyces silaceus, Streptomyces flavofungini and Streptomyces intermedius. IJSEM 66: 44 - 49 2016 ( DOI 10.1099/ijsem.0.000671 , PubMed 26449519 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Streptomyces alfalfae (DSM 103384, CCTCC AA 2015019, KCTC 39571)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Fatty acid profile

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_004127445

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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