Name: Bacillus cytotoxicus 391-98
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1323

Type strain:

Species: Bacillus cytotoxicus

Strain Designation: 391-98

Culture col. no.: DSM 22905, CIP 110041, NVH 391-98, WDCM 00220

Strain history: CIP <- 2009, D. Lereclus, INRA, Jouy en Josas, France <- M.L. de Buyser, AFSSA LERQAP, Maisons-Alfort, France: strain NVH 391-98

NCBI tax ID(s): 315749 (strain), 580165 (species)

SI-ID 378380

LMG 26718, CIP 110041, DSM 22905

Section

Bacillus cytotoxicus 391-98 is a facultative anaerobe, spore-forming, mesophilic human pathogen that forms circular colonies and was isolated from vegetable puree, related to food poisoning.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
human pathogen
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus cytotoxicus**
	Full Scientific Name (LPSN)	Bacillus cytotoxicus Guinebretière et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #23142]	Gram stain	positive
[Ref.: #118837]	Gram stain	positive

[Ref.: #23142]	Cell length	>1.0 µm

[Ref.: #23142]	Cell shape	rod-shaped
[Ref.: #118837]	Cell shape	rod-shaped

[Ref.: #23142]	Motility	yes
[Ref.: #118837]	Motility	no

[Ref.: #23142]	Colony size	1.0 mm
[Ref.: #23142]	Colony color	cream
[Ref.: #23142]	Colony shape	circular
[Ref.: #23142]	Incubation period	1 day
[Ref.: #23142]	Cultivation medium used	TSA

Information on culture and growth conditions Culture and growth conditions

[Ref.: #23142]

Culture medium

Trypticase Soy Agar (TSA)

[Ref.: #23142]

Culture medium growth

[Ref.: #33672]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #33672]

Culture medium growth

[Ref.: #33672]

Culture medium composition

expand / minimize

[Ref.: #16614]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #16614]

Culture medium growth

[Ref.: #16614]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16614]

Culture medium composition

expand / minimize

[Ref.: #16614]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #16614]

Culture medium growth

[Ref.: #16614]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16614]

Culture medium composition

expand / minimize

[Ref.: #118837]

Culture medium

CIP Medium 72

[Ref.: #118837]

Culture medium growth

[Ref.: #118837]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16614]	growth	37 °C
[Ref.: #23142]	maximum	50 °C
[Ref.: #23142]	minimum	20 °C
[Ref.: #23142]	optimum	30-37 °C
[Ref.: #33672]	growth	30 °C

[Ref.: #16614]	Temperature range	mesophilic
[Ref.: #23142]	Temperature range	thermophilic
[Ref.: #23142]	Temperature range	psychrophilic
[Ref.: #23142]	Temperature range	mesophilic
[Ref.: #33672]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #118837]

Oxygen tolerance

facultative anaerobe

[Ref.: #118837]

Ability of spore formation

yes

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23142]	amygdalin ChEBI	+/-	carbon source
[Ref.: #23142]	arbutin ChEBI	+	carbon source
[Ref.: #23142]	cellobiose ChEBI	+	carbon source
[Ref.: #23142]	D-mannose ChEBI	+	assimilation
[Ref.: #23142]	D-mannose ChEBI	+	carbon source
[Ref.: #23142]	galactose ChEBI	-	carbon source
[Ref.: #23142]	gelatin ChEBI	+
[Ref.: #23142]	gentiobiose ChEBI	-	carbon source
[Ref.: #23142]	gluconate ChEBI	-	carbon source
[Ref.: #23142]	glycerol ChEBI	-	carbon source
[Ref.: #23142]	glycogen ChEBI	-	assimilation
[Ref.: #23142]	glycogen ChEBI	-	carbon source
[Ref.: #23142]	hydrogen sulfide ChEBI	-	other
[Ref.: #23142]	methyl D-glucoside ChEBI	-	carbon source
[Ref.: #23142]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #23142]	nitrate ChEBI	+	reduction
[Ref.: #118837]	nitrate ChEBI	+	reduction
[Ref.: #118837]	nitrite ChEBI	+	reduction
[Ref.: #23142]	ribose ChEBI	+	carbon source
[Ref.: #23142]	salicin ChEBI	+	carbon source
[Ref.: #23142]	starch ChEBI	-	carbon source
[Ref.: #23142]	starch ChEBI	-	hydrolysis
[Ref.: #23142]	sucrose ChEBI	-	assimilation
[Ref.: #23142]	sucrose ChEBI	-	carbon source
[Ref.: #23142]	trehalose ChEBI	-	assimilation
[Ref.: #23142]	trehalose ChEBI	-	carbon source
[Ref.: #23142]	tryptophan ChEBI	-	carbon source
[Ref.: #23142]	tryptophan ChEBI	-	energy source
[Ref.: #23142]	turanose ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #23142]	acetoin ChEBI	yes
[Ref.: #23142]	indole ChEBI	no
[Ref.: #118837]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #23142]		acetoin ChEBI	+
[Ref.: #23142]		indole ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118837]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #23142]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #23142]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #118837]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #23142]	tryptophan deaminase	-	4.1.99.1
[Ref.: #23142]	urease	-	3.5.1.5
[Ref.: #118837]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118837]	12315	-	-	+	-	-	+	+	+	-	+	+	+	-	-	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16614]	Sample type/isolated from	vegetable puree, related to food poisoning
[Ref.: #16614]	Country	France
[Ref.: #16614]	Country ISO 3 Code	FRA
[Ref.: #16614]	Continent	Europe

[Ref.: #118837]	Sample type/isolated from	Food, Vegetable
[Ref.: #118837]	Country	France
[Ref.: #118837]	Country ISO 3 Code	FRA
[Ref.: #118837]	Continent	Europe
[Ref.: #118837]	Isolation date	1998
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Food
#Host	#Plants	-
#Host Body-Site	#Plant	#Fruit (Seed)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16614]	Pathogenicity (human)	yes, in single cases
[Ref.: #16614]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118837]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16614]	Bacillus cereus partial 16S rRNA gene and ITS1, strain NVH 391-98	AM747234	1765	ENA	1396 tax ID

[Ref.: #16614]

GC-content

35.8 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #16614]

Culture collection no.

DSM 22905, CIP 110041, NVH 391-98, WDCM 00220

[Ref.: #70973]

SI-ID 378380

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Genetics	Bacillus cytotoxicus Genomics: Chromosomal Diversity and Plasmidome Versatility.	Fayad N, Kone KM, Gillis A, Mahillon J	Front Microbiol	10.3389/fmicb.2021.789929	2021	*
Genetics	Draft Genome Sequence of CH_213, a Highly Cytotoxic Bacillus cytotoxicus Strain Isolated from Mashed Potatoes.	Stevens MJA, Johler S	Microbiol Resour Announc	10.1128/MRA.00836-20	2020	*
Genetics	Draft Genome Sequence of Bacillus cytotoxicus CVUAS 2833, a Very Close Relative to Type Strain NVH 391-98 Isolated from a Different Location.	Bohm ME, Huptas C, Krey VM, Scherer S	Genome Announc	10.1128/genomeA.00901-15	2015	*
Metabolism	Is Cytotoxin K from Bacillus cereus a bona fide enterotoxin?	Castiaux V, Liu X, Delbrassinne L, Mahillon J	Int J Food Microbiol	10.1016/j.ijfoodmicro.2015.06.020	2015	*
Phylogeny	Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus Group occasionally associated with food poisoning.	Guinebretiere MH, Auger S, Galleron N, Contzen M, De Sarrau B, De Buyser ML, Lamberet G, Fagerlund A, Granum PE, Lereclus D, De Vos P, Nguyen-The C, Sorokin A	Int J Syst Evol Microbiol	10.1099/ijs.0.030627-0	2012	*
Genetics	Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity.	Lapidus A, Goltsman E, Auger S, Galleron N, Segurens B, Dossat C, Land ML, Broussolle V, Brillard J, Guinebretiere MH, Sanchis V, Nguen-The C, Lereclus D, Richardson P, Wincker P, Weissenbach J, Ehrlich SD, Sorokin A	Chem Biol Interact	10.1016/j.cbi.2007.03.003	2007	*

References

#16614

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22905

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23142

Marie-Hélène Guinebretière, Sandrine Auger, Nathalie Galleron, Matthias Contzen, Benoit De Sarrau, Marie-Laure De Buyser, Gilles Lamberet, Annette Fagerlund, Per Einar Granum, Didier Lereclus, Paul De Vos, Christophe Nguyen-The, Alexei Sorokin: Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus Group occasionally associated with food poisoning. IJSEM 63: 31 - 40 2013 ( DOI 10.1099/ijs.0.030627-0 , PubMed 22328607 )

#33672 ; Curators of the CIP;

#68382

Automatically annotated from API zym .

#70973

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID378380.1 )

#118837 Collection of Institut Pasteur ; Curators of the CIP; CIP 110041
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Bacillus cytotoxicus in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF