Name: Rhizobium binae BLR195
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132245

Type strain:

Species: Rhizobium binae

Strain Designation: BLR195

Culture col. no.: DSM 29288, LMG 28443

Strain history: <- Md. Harun-or Rashid, Bangladesh Institute of Nuclear agriculture, Mymensingh; BLR195

NCBI tax ID(s): 1138190 (species)

SI-ID 396482

LMG 28443, DSM 29288

Section

Rhizobium binae BLR195 is a mesophilic bacterium that was isolated from effective root nodules of lentil .

mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Rhizobiaceae*
	Genus	*Rhizobium*
	Species	**Rhizobium binae**
	Full Scientific Name (LPSN)	Rhizobium binae Rashid et al. 2015

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24034]

Culture medium

RHIZOBIUM MEDIUM (DSMZ Medium 98)

[Ref.: #24034]

Culture medium growth

[Ref.: #24034]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24034]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #24034]			growth	30 °C

[Ref.: #24034]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	+	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	+	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #24034]	24184	+/-	-	-	-	+	+/-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24034]	Sample type/isolated from	effective root nodules of lentil (Lens culinaris)
[Ref.: #24034]	Host species	Lens culinaris
[Ref.: #24034]	Geographic location (country and/or sea, region)	Feni district, Sarishadi
[Ref.: #24034]	Country	Bangladesh
[Ref.: #24034]	Country ISO 3 Code	BGD
[Ref.: #24034]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JN648932 (>99% sequence identity) for Rhizobium from Microbeatlas

Aquatic Samples	32
Soil Samples	50
Animal Samples	28
Plant Samples	223
Total Samples	333

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24034]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24034]	Rhizobium binae strain BLR195 16S ribosomal RNA gene, partial sequence	JN648932	1469	ENA	1138190 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Rhizobium binae BLR195	GCA_017357225	complete	GenBank	1138190 tax ID	*
[Ref.: #66792]	Rhizobium binae BLR195	GCA_019684455	contig	GenBank	1138190 tax ID	*
[Ref.: #66792]	Rhizobium binae DSM 29288	2928991799	draft	JGI IMG/M	1138190 tax ID	*

[Ref.: #24034]

GC-content

61.5 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Rhizobium binae BLR195 NCBI: GCA_017357225.1
[Ref.: #69480]	flagellated	no	85.089	no
[Ref.: #69480]	gram-positive	no	97.833	no
[Ref.: #69480]	anaerobic	no	98.296	no
[Ref.: #69480]	aerobic	yes	92.28	no
[Ref.: #69480]	halophile	no	93.62	no
[Ref.: #69480]	spore-forming	no	93.852	no
[Ref.: #69480]	thermophile	no	99.286	yes
[Ref.: #69480]	glucose-util	yes	85.783	no
[Ref.: #69480]	motile	yes	82.905	no
[Ref.: #69480]	glucose-ferment	no	89.175	yes

Availability in culture collections External links

[Ref.: #24034]

Culture collection no.

DSM 29288, LMG 28443

[Ref.: #90595]

SI-ID 396482

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	The symbiovar trifolii of Rhizobium bangladeshense and Rhizobium aegyptiacum sp. nov. nodulate Trifolium alexandrinum in Egypt.	Shamseldin A, Carro L, Peix A, Velazquez E, Moawad H, Sadowsky MJ	Syst Appl Microbiol	10.1016/j.syapm.2016.05.002	2016	*
Phylogeny	Average nucleotide identity of genome sequences supports the description of Rhizobium lentis sp. nov., Rhizobium bangladeshense sp. nov. and Rhizobium binae sp. nov. from lentil (Lens culinaris) nodules.	Rashid MH, Young JPW, Everall I, Clercx P, Willems A, Santhosh Braun M, Wink M	Int J Syst Evol Microbiol	10.1099/ijs.0.000373	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24034

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29288

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#90595

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID396482.1 )

* These data were automatically processed and therefore are not curated

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