Name: Rahnella woolbedingensis ALN24
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132222

Type strain:

Species: Rahnella woolbedingensis

Strain Designation: ALN24

Culture col. no.: DSM 27399, CIP 111497, LMG 27719, FRB 227

Strain history: CIP <- 2017, DSMZ <- C. Brady, Forest Res., Surrey, UK: strain FRB 227 <- S. Denman, Forest Res., Surrey, UK: strain ALN24

NCBI tax ID(s): 1510574 (species)

SI-ID 391490

LMG 27719, DSM 27399, CIP 111497

Section

Rahnella woolbedingensis ALN24 is a mesophilic, Gram-negative bacterium that was isolated from Alnus glutinosa.

Gram-negative
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Yersiniaceae*
	Genus	*Rahnella*
	Species	**Rahnella woolbedingensis**
	Full Scientific Name (LPSN)	Rahnella woolbedingensis Brady et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #123959]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.981

[Ref.: #123959]	Cell shape	rod-shaped

[Ref.: #123959]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %98.144

Multimedia content

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24011]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

[Ref.: #123959]

Culture medium

CIP Medium 328

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #24011]			growth	28 °C

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Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.556

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	-	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #24011]	24667	+	-	+	-	-	+	-	+	+	+	+	+	+	+	+	-	-	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24011]	Sample type/isolated from	Alnus glutinosa
[Ref.: #24011]	Host species	Alnus glutinosa
[Ref.: #24011]	Geographic location (country and/or sea, region)	Surrey
[Ref.: #24011]	Country	United Kingdom
[Ref.: #24011]	Country ISO 3 Code	GBR
[Ref.: #24011]	Continent	Europe

[Ref.: #123959]	Sample type/isolated from	Alnus glutinosa
[Ref.: #123959]	Geographic location (country and/or sea, region)	Surrey
[Ref.: #123959]	Country	United Kingdom
[Ref.: #123959]	Country ISO 3 Code	GBR
[Ref.: #123959]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host

#Plants

#Tree

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KF308409 (>99% sequence identity) for Yersiniaceae from Microbeatlas

Aquatic Samples	435
Soil Samples	99
Animal Samples	935
Plant Samples	200
Total Samples	1669

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24011]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123959]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24011]	Rahnella woolbedingensis strain FRB 227 16S ribosomal RNA gene, complete sequence	KF308409	1345	ENA	1510574 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Rahnella woolbedingensis DSM 27399	GCA_003602095	contig	GenBank	1510574 tax ID	*
[Ref.: #66792]	Rahnella woolbedingensis strain DSM 27399	1510574.3	wgs	PATRIC	1510574 tax ID	*
[Ref.: #66792]	Rahnella woolbedingensis DSM 27399	2846381357	draft	JGI IMG/M	1510574 tax ID	*

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GC-content

51.4 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Rahnella woolbedingensis strain DSM 27399 PATRIC: 1510574.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Rahnella woolbedingensis strain DSM 27399 PATRIC: 1510574.3
[Ref.: #69480]	motile	yes	91.627	no
[Ref.: #69480]	gram-positive	no	97.679	no
[Ref.: #69480]	anaerobic	no	98.417	no
[Ref.: #69480]	aerobic	yes	76.141	no
[Ref.: #69480]	halophile	no	89.509	no
[Ref.: #69480]	spore-forming	no	95.765	no
[Ref.: #69480]	thermophile	no	99.678	yes
[Ref.: #69480]	glucose-util	yes	95.796	yes
[Ref.: #69480]	flagellated	no	60.761	no
[Ref.: #69480]	glucose-ferment	yes	91.623	no

Availability in culture collections External links

[Ref.: #24011]

Culture collection no.

DSM 27399, CIP 111497, LMG 27719, FRB 227

[Ref.: #90572]

SI-ID 391490

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rahnella victoriana sp. nov., Rahnella bruchi sp. nov., Rahnella woolbedingensis sp. nov., classification of Rahnella genomospecies 2 and 3 as Rahnella variigena sp. nov. and Rahnella inusitata sp. nov., respectively and emended description of the genus Rahnella.	Brady C, Hunter G, Kirk S, Arnold D, Denman S	Syst Appl Microbiol	10.1016/j.syapm.2014.09.001	2014	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24011

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27399

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90572

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID391490.1 )

#123959 Collection of Institut Pasteur ; Curators of the CIP; CIP 111497
* These data were automatically processed and therefore are not curated

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Rahnella woolbedingensis strain DSM 27399

Rahnella woolbedingensis strain DSM 27399

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