Name: Staphylococcus pragensis NRL/St 12/356
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132215

Type strain:

Species: Staphylococcus pragensis

Strain Designation: NRL/St 12/356, P5397

Culture col. no.: DSM 102853, CCM 8529, LMG 28327, CCUG 72270

Strain history: <- CCM <- P. Petrás; NRL/St 12/356

NCBI tax ID(s): 1276936 (species)

SI-ID 396400

LMG 28327, CCM 8529, CCUG 72270

Section

Staphylococcus pragensis NRL/St 12/356 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from Ejaculate .

Gram-positive
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Staphylococcaceae*
	Genus	*Staphylococcus*
	Species	**Staphylococcus pragensis**
	Full Scientific Name (LPSN)	Staphylococcus pragensis (Švec et al. 2015) Madhaiyan et al. 2020

Synonym

Staphylococcus petrasii subsp. pragensis

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24004]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #24004]

Culture medium growth

[Ref.: #24004]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24004]

Culture medium composition

expand / minimize

[Ref.: #24004]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #24004]

Culture medium growth

[Ref.: #24004]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24004]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #24004]			growth	37 °C
[Ref.: #63799]			growth	37 °C

[Ref.: #24004]	Temperature range	mesophilic
[Ref.: #63799]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #63799]

Oxygen tolerance

aerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.67

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68375]	arabinose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	arginine ChEBI	-	hydrolysis	* from API ID32STA, API STA
[Ref.: #68375]	cellobiose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68378]	D-fructose ChEBI	+	builds acid from	* from API STA
[Ref.: #68375]	D-fructose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68379]	D-glucose ChEBI	+	fermentation	* from API Coryne, API ID32STA
[Ref.: #68378]	D-glucose ChEBI	-	builds acid from	* from API STA
[Ref.: #68378]	D-mannitol ChEBI	+	builds acid from	* from API STA
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne, API ID32STA
[Ref.: #68378]	D-mannose ChEBI	-	builds acid from	* from API STA
[Ref.: #68375]	D-mannose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne, API ID32STA
[Ref.: #68378]	D-xylose ChEBI	-	builds acid from	* from API STA
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne, API ID32STA
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68378]	lactose ChEBI	+	builds acid from	* from API STA
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne, API ID32STA
[Ref.: #68379]	maltose ChEBI	+	fermentation	* from API Coryne, API ID32STA
[Ref.: #68378]	maltose ChEBI	-	builds acid from	* from API STA
[Ref.: #68378]	melibiose ChEBI	-	builds acid from	* from API STA
[Ref.: #68378]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from	* from API STA
[Ref.: #68378]	N-acetylglucosamine ChEBI	-	builds acid from	* from API STA
[Ref.: #68375]	N-acetylglucosamine ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne, API ID32STA, API STA
[Ref.: #68375]	ornithine ChEBI	-	degradation	* from API ID32STA
[Ref.: #68378]	raffinose ChEBI	+	builds acid from	* from API STA
[Ref.: #68375]	raffinose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne, API ID32STA
[Ref.: #68378]	sucrose ChEBI	-	builds acid from	* from API STA
[Ref.: #68375]	trehalose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68378]	trehalose ChEBI	-	builds acid from	* from API STA
[Ref.: #68375]	turanose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne, API ID32STA, API STA
[Ref.: #68378]	xylitol ChEBI	-	builds acid from	* from API STA
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #68378]		lysostaphin	yes			* from API STA
[Ref.: #68375]		novobiocin ChEBI	yes	1.8	µg	* from API ID32STA

	Metabolite production	Metabolite	Production
[Ref.: #68378]		acetoin ChEBI	no	* from API STA, API ID32STA

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68375]		acetoin ChEBI	+	* from API ID32STA
[Ref.: #68378]		acetoin ChEBI	-	* from API STA

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68378]	alkaline phosphatase	-	3.1.3.1	* from API STA, API ID32STA, API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68378]	arginine dihydrolase	-	3.5.3.6	* from API STA, API ID32STA
[Ref.: #68375]	beta-galactosidase	-	3.2.1.23	* from API ID32STA, API Coryne, API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68375]	beta-glucosidase	-	3.2.1.21	* from API ID32STA, API Coryne
[Ref.: #68375]	beta-glucuronidase	-	3.2.1.31	* from API ID32STA, API Coryne, API zym
[Ref.: #68379]	catalase	+	1.11.1.6	* from API Coryne
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68375]	L-arginine arylamidase	-		* from API ID32STA
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68375]	ornithine decarboxylase	-	4.1.1.17	* from API ID32STA
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68375]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API ID32STA, API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68378]	urease	-	3.5.1.5	* from API STA, API ID32STA, API Coryne
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #63799]	3155	-	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	+	-	+

API® ID32STA:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	Ornithine decarboxylaseODC	Esculin hydrolysisESC	Fermentation of D-glucoseGLU	Fermentation of D-fructoseFRU	Fermentation of D-mannoseMNE	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of trehaloseTRE	Fermentation of D-mannitolMAN	Fermentation of raffinoseRAF	Fermentation of D-riboseRIB	Fermentation of cellobioseCEL	Reduction of nitrateNIT	Acetoin production (Voges Proskauer test)VP	beta-Galactosidasebeta GAL	L-arginine arylamidaseArgA	Alkaline phosphatasePAL	Pyrrolidonyl arylamidasePyrA	Novobiocin resistanceNOVO	Fermentation of sucroseSAC	Fermentation of N-acetyl-glucosamineNAG	Fermentation of turanoseTUR	Fermentation of arabinoseARA	beta-Glucuronidasebeta GUR
[Ref.: #24004]	6232	-	-	-	-	+	-	-	+	-	+	-	-	-	-	-	+	-	-	-	+	-	+	-	-	-	-
[Ref.: #63799]	2461	-	-	-	-	+	-	-	+	-	+	-	-	-	-	-	+	-	-	-	+	-	+	-	-	-	-

API® STA:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from maltoseMAL	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from D-mannitolMAN	Acid from xylitolXLT	Acid from melibioseMEL	Reduction of nitrateNIT	Alkaline phosphatasePAL	Acetoin production (Voges Proskauer test)VP	Acid from raffinoseRAF	Acid from D-xyloseXYL	Acid from sucroseSAC	Acid from methyl-alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Arginine dihydrolaseADH	Urease/urea hydrolysisURE	Resistance to lysostaphin (EC 3.4.24.75)LSTR
[Ref.: #63799]	222	not determinedn.d.	-	+	-	-	+	-	+	-	-	-	-	-	+	-	-	+	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #63799]	4690	-	-	+	+	-	-	-	-	-	-	+	-	-	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24004]	Sample type/isolated from	Ejaculate (58-year-old patient with chronical prostatitis)
[Ref.: #24004]	Geographic location (country and/or sea, region)	Prague
[Ref.: #24004]	Country	Czech Republic
[Ref.: #24004]	Country ISO 3 Code	CZE
[Ref.: #24004]	Continent	Europe

[Ref.: #63799]	Sample type/isolated from	Human ejaculate,chronical prostatitis
[Ref.: #63799]	Sampling date	2014
[Ref.: #63799]	Geographic location (country and/or sea, region)	Prague
[Ref.: #63799]	Country	Czech Republic
[Ref.: #63799]	Country ISO 3 Code	CZE
[Ref.: #63799]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Infection	#Patient	-
#Host Body-Site	#Urogenital tract	-
#Host Body Product	#Urogenital tract	#Semen
#Host	#Human	#Male

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24004]	Staphylococcus petrasii subsp. pragensis strain CCM 8529 16S ribosomal RNA gene, partial sequence	KM873669	1480	ENA	1611836 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Staphylococcus pragensis CCM 8529	GCA_004785665	scaffold	GenBank	1611836 tax ID	*
[Ref.: #66792]	Staphylococcus pragensis CCM 8529	GCA_014635505	contig	GenBank	1611836 tax ID	*
[Ref.: #66792]	Staphylococcus petrasii subsp. pragensis strain CCM 8529	1611836.5	wgs	PATRIC	1611836 tax ID	*
[Ref.: #66792]	Staphylococcus petrasii subsp. pragensis strain CCM 8529	1611836.4	wgs	PATRIC	1611836 tax ID	*
[Ref.: #66792]	Staphylococcus petrasii subsp. pragensis strain DSM 102853	1611836.3	wgs	PATRIC	1611836 tax ID	*

[Ref.: #24004]

GC-content

33.0 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Staphylococcus pragensis CCM 8529 NCBI: GCA_004785665.1
[Ref.: #69481]	spore-forming	no	81	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Staphylococcus pragensis CCM 8529 NCBI: GCA_004785665.1
[Ref.: #69480]	motile	no	88.03	no
[Ref.: #69480]	flagellated	no	90.497	no
[Ref.: #69480]	gram-positive	yes	90.349	no
[Ref.: #69480]	anaerobic	no	99.124	yes
[Ref.: #69480]	aerobic	yes	92.511	no
[Ref.: #69480]	halophile	yes	96.226	no
[Ref.: #69480]	spore-forming	no	90.445	no
[Ref.: #69480]	glucose-util	yes	87.779	no
[Ref.: #69480]	thermophile	no	99.801	no
[Ref.: #69480]	glucose-ferment	yes	77.894	yes

Availability in culture collections External links

[Ref.: #24004]

Culture collection no.

DSM 102853, CCM 8529, LMG 28327, CCUG 72270

[Ref.: #90568]

SI-ID 396400

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24004

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 102853

#63799 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 72270

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68375

Automatically annotated from API ID32STA .

#68378

Automatically annotated from API STA .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90568

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID396400.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Staphylococcus pragensis in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Staphylococcus pragensis CCM 8529

Staphylococcus pragensis CCM 8529

Change proposal

Change proposal