Name: Nocardia mediterranei Lepetit ME/83
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13220

Type strain:

Species: Nocardia mediterranei

Strain Designation: Lepetit ME/83, Lepetit ME83/973, Schering 368

Culture col. no.: DSM 43304, ATCC 13685, CBS 121.63, CBS 716.72, DSM 40501, IFO 13142, IFO 13415, IMET 7651, ISP 5501, JCM 4789, KCC S-0789, LBG A 3136, NBRC 13142, NBRC 13415, NCIB 9613, NRRL B-3240, PSA 71, RIA 1376, VKM Ac-798, CIP 107074, CCUG 43144, ETH 28579, BCRC 12440, CGMCC 4.1213, IMSNU 20056, KCCM 11859, KCTC 1739, MTCC 14, NBIMCC 3395, NCIMB 9613, PCM 2320

Strain history: CIP <- 2001, CCUG <- 2000, DSMZ <- K. Kieslich: strain Schering 368 <- ATCC <- Lepetit Labs: strain ME 83/973, Streptomyces mediterranei

NCBI tax ID(s): 33910 (species)

SI-ID 45544

ATCC 13685, IFO 13415, IMET 7651, NRRL B-3240, DSM 43304, DSM 40501, JCM 4789, NCIB 9613, VKM Ac-798, CCRC 12440, CCUG 11119, IFO 13142, NCIMB 9613, CBS 121.63, CBS 716.72, KCTC 1739, CIP 107074, CCUG 43144, NBRC 13142, NBRC 13415, BCRC 12440, NBIMCC 3395, NCIM 5008, CGMCC 4.1213, ATCC13

Section

Nocardia mediterranei Lepetit ME/83 is an aerobe, mesophilic, Gram-positive bacterium that produces antibiotic compounds and was isolated from soil.

antibiotic compound production
filament-shaped
Gram-positive
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Nocardiaceae*
	Genus	*Nocardia*
	Species	Nocardia mediterranei
	Full Scientific Name (LPSN)	Nocardia mediterranei corrig. (Margalith and Beretta 1960) Thiemann et al. 1969 (Approved Lists 1980)

	Synonym	Nocardia mediterranea
	Synonym	**Amycolatopsis mediterranei**
	Synonym	"Streptomyces* mediterranei"*

Information on morphological and physiological properties Morphology

[Ref.: #120876]	Gram stain	positive

[Ref.: #120876]	Cell shape	filament-shaped

[Ref.: #120876]	Motility	no

Multimedia content

[Ref.: #10944]

Caption:

Medium 65 28°C

[Ref.: #10944]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #10944]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #10944]

Culture medium growth

[Ref.: #10944]

Culture medium link

Medium recipe at MediaDive

[Ref.: #10944]

Culture medium composition

expand / minimize

[Ref.: #39652]

Culture medium

MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum

[Ref.: #39652]

Culture medium growth

[Ref.: #39652]

Culture medium composition

expand / minimize

[Ref.: #120876]

Culture medium

CIP Medium 57

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Culture medium growth

[Ref.: #120876]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #10944]		growth	28 °C
[Ref.: #39652]		growth	30 °C
[Ref.: #55691]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #120876]		growth	25-37 °C
[Ref.: #120876]	no	growth	10 °C
[Ref.: #120876]	no	growth	41 °C
[Ref.: #120876]	no	growth	45 °C

[Ref.: #10944]	Temperature range	mesophilic
[Ref.: #39652]	Temperature range	mesophilic
[Ref.: #55691]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #120876]	Temperature range	mesophilic
[Ref.: #120876]	Temperature range	psychrophilic
[Ref.: #120876]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #55691]	Oxygen tolerance	aerobe
[Ref.: #120876]	Oxygen tolerance	obligate aerobe

[Ref.: #10944]	Name of produced compound	rifamycin SV
[Ref.: #20216]	Name of produced compound	Rifamycin SV

	Salt	Tested relation		Salt conc.
[Ref.: #120876]	NaCl		growth	0-4	%
[Ref.: #120876]	NaCl	no	growth	6	%
[Ref.: #120876]	NaCl	no	growth	8	%
[Ref.: #120876]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #120876]	citrate ChEBI	-	carbon source
[Ref.: #120876]	esculin ChEBI	+	hydrolysis
[Ref.: #120876]	hippurate ChEBI	+	hydrolysis
[Ref.: #120876]	nitrate ChEBI	-	reduction
[Ref.: #120876]	nitrate ChEBI	-	respiration
[Ref.: #120876]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120876]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #120876]		indole ChEBI	no
[Ref.: #67770]		rifamycin ChEBI	yes

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #120876]		acetoin ChEBI	-
[Ref.: #120876]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120876]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120876]	amylase	-
[Ref.: #120876]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120876]	caseinase	+	3.4.21.50
[Ref.: #120876]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #120876]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #120876]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #120876]	gelatinase	+/-
[Ref.: #120876]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #120876]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120876]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120876]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120876]	oxidase	-
[Ref.: #120876]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #120876]	tryptophan deaminase	-
[Ref.: #120876]	tween esterase	-
[Ref.: #120876]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #120876]	1987	not determinedn.d.	+	+	+	+	+	-	-	+	-	+	+	-	+	+	-	+	+	-	+	+	+	+	-	+	+	+	+	-	-	-	+	+	+	+	-	-	-	-	-	+	-	-	-	+	-	-	-	-	-	+	+	+	+	-	+	-	-	-	-	+	+	-	+	-	+	+	+	-	-	+	+	+	-	+	+	+	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+	-	-	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120876]	10042	-	+	+	+	-	+	+	+	+	+	+	+	+	+	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #10944]	Sample type/isolated from	soil

[Ref.: #55691]	Sample type/isolated from	Soil

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	St. Raphael
[Ref.: #67770]	Country	France
[Ref.: #67770]	Country ISO 3 Code	FRA
[Ref.: #67770]	Continent	Europe

[Ref.: #120876]	Sample type/isolated from	Environment, Soil
[Ref.: #120876]	Country	France
[Ref.: #120876]	Country ISO 3 Code	FRA
[Ref.: #120876]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ293754 (>99% sequence identity) for Amycolatopsis from Microbeatlas

Aquatic Samples	156
Soil Samples	4583
Animal Samples	306
Plant Samples	815
Total Samples	5860

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #10944]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19565]	Biosafety level	1	Risk group (German classification)
[Ref.: #120876]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	A.mediterranei (DSM 13685) 16S ribosomal RNA (partial)	X76957	1426	ENA	33910 tax ID
[Ref.: #20218]	Amycolatopsis mediterranei partial 16S rRNA gene, strain IMSNU 20056T	AJ293754	1488	ENA	33910 tax ID	*
[Ref.: #20218]	Amycolatopsis mediterranei gene for 16S ribosomal RNA, partial sequence, strain: JCM 4789	D44364	120	ENA	33910 tax ID	*
[Ref.: #20218]	Amycolatopsis mediterranei strain KCTC 1739T 16S ribosomal RNA gene, partial sequence	AY125600	1413	ENA	33910 tax ID	*
[Ref.: #20218]	Amycolatopsis mediterranei strain NRRL B-3240 16S ribosomal RNA gene, partial sequence	AY184424	1482	ENA	33910 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Amycolatopsis mediterranei S699	GCA_000220945	complete	GenBank	713604 tax ID
[Ref.: #66792]	Amycolatopsis mediterranei S699, ATCC 13685	2511231181	complete	JGI IMG/M	713604 tax ID	*
[Ref.: #66792]	Amycolatopsis mediterranei ISP5501	GCA_026017845	chromosome	GenBank	33910 tax ID	*

[Ref.: #67770]	GC-content	67-69 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	71.3 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	70 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #10944]

Culture collection no.

DSM 43304, ATCC 13685, CBS 121.63, CBS 716.72, DSM 40501, IFO 13142, IFO 13415, IMET 7651, ISP 5501, JCM 4789, KCC S-0789, LBG A 3136, NBRC 13142, NBRC 13415, NCIB 9613, NRRL B-3240, PSA 71, RIA 1376, VKM Ac-798, CIP 107074, CCUG 43144, ETH 28579, BCRC 12440, CGMCC 4.1213, IMSNU 20056, KCCM 11859, KCTC 1739, MTCC 14, NBIMCC 3395, NCIMB 9613, PCM 2320

[Ref.: #82427]

SI-ID 45544

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Biotechnology	Improved Rifamycin B Production by Nocardia mediterranei MTCC 14 under Solid-State Fermentation through Process Optimization.	Vastrad BM, Neelagund SE, Iiger SR, Godbole AM, Kulkarni V	Biochem Res Int	10.1155/2014/621309	2014	*
Enzymology	Transformation system for Amycolatopsis (Nocardia) mediterranei: direct transformation of mycelium with plasmid DNA.	Madon J, Hutter R	J Bacteriol	10.1128/jb.173.20.6325-6331.1991	1991	*
Genetics	Amplification on the Amycolatopsis (Nocardia) mediterranei plasmid pMEA100: sequence similarities to actinomycete att sites.	Zhu B, Madon J, Hausler A, Hutter R	Plasmid	10.1016/0147-619x(90)90015-5	1990	*
Enzymology	Site-specific integration and excision of pMEA100 in Nocardia mediterranei.	Madon J, Moretti P, Hutter R	Mol Gen Genet	10.1007/BF00329651	1987	*
Phylogeny	Isolation and characterization of an extrachromosomal element from Nocardia mediterranei.	Moretti P, Hintermann G, Hutter R	Plasmid	10.1016/0147-619x(85)90072-1	1985	*
Metabolism	Genetic recombination in Nocardia mediterranei.	Schupp T, Hutter R, Hopwood DA	J Bacteriol	10.1128/jb.121.1.128-136.1975	1975	*
Enzymology	Development of an improved cloning vector and transformation system in Amycolatopsis mediterranei (Nocardia mediterranei).	Lal R, Khanna R, Dhingra N, Khanna M, Lal S	J Antibiot (Tokyo)	10.7164/antibiotics.51.161	1998	*

References

#10944

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43304

#19565

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20216

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39652 ; Curators of the CIP;
#55691 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 43144

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#82427

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID45544.1 )

#120876 Collection of Institut Pasteur ; Curators of the CIP; CIP 107074
* These data were automatically processed and therefore are not curated

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