Name: Gilliamella apicola wkB1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132150

Type strain:

Species: Gilliamella apicola

Strain Designation: wkB1

Culture col. no.: DSM 104097, ATCC BAA-2448, NCIMB 14804

Strain history: <- NCIMB <- W. Kwong, Department of Ecology and Evolutionary Biology, Yale University, New Haven, USA

NCBI tax ID(s): 1196095 (species)

SI-ID 397885

DSM 104097

Section

Gilliamella apicola wkB1 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from gut of honey bee (Apis mellifera.

Gram-negative
motile
rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Orbales*
	Family	*Orbaceae*
	Genus	*Gilliamella*
	Species	**Gilliamella apicola**
	Full Scientific Name (LPSN)	Gilliamella apicola Kwong and Moran 2013

Synonym

"Candidatus Gilliamella apicola"

Information on morphological and physiological properties Morphology

[Ref.: #30807]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.619

[Ref.: #30807]	Cell length	1.5 µm

[Ref.: #30807]	Cell width	0.5 µm

[Ref.: #30807]	Cell shape	rod-shaped

[Ref.: #30807]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %92.826

[Ref.: #30807]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #23940]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #23940]

Culture medium growth

[Ref.: #23940]

Culture medium link

Medium recipe at MediaDive

[Ref.: #23940]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #23940]			growth	37 °C
[Ref.: #30807]			optimum	37 °C

[Ref.: #23940]	Temperature range	mesophilic
[Ref.: #30807]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30807]			growth	6.0-6.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30807]

Oxygen tolerance

microaerophile

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.971

[Ref.: #30807]

Observation

aggregates in clumps

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23940]	Sample type/isolated from	gut of honey bee (Apis mellifera
[Ref.: #23940]	Host species	Apis mellifera
[Ref.: #23940]	Geographic location (country and/or sea, region)	West Haven
[Ref.: #23940]	Country	USA
[Ref.: #23940]	Country ISO 3 Code	USA
[Ref.: #23940]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #23940]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #30807]	Gilliamella apicola strain wkB1 16S ribosomal RNA gene, complete sequence	JQ936674	1538	GenBank	1196095 tax ID
[Ref.: #23940]		JQ93674		ENA

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Gilliamella apicola wkB1	GCA_000599985	complete	GenBank	1196095 tax ID	*
[Ref.: #66792]	Gilliamella apicola DSM 104097	GCA_003201455	scaffold	GenBank	1196095 tax ID	*
[Ref.: #66792]	Gilliamella apicola strain DSM 104097	1196095.342	wgs	PATRIC	1196095 tax ID	*
[Ref.: #66792]	Gilliamella apicola wkB1	2574179809	complete	JGI IMG/M	1196095 tax ID	*
[Ref.: #66792]	Gilliamella apicola DSM 104097	2756170265	draft	JGI IMG/M	1196095 tax ID	*

[Ref.: #30807]

GC-content

33.6 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Gilliamella apicola wkB1 NCBI: GCA_000599985.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Gilliamella apicola wkB1 NCBI: GCA_000599985.1
[Ref.: #69480]	motile	yes	68.715	no
[Ref.: #69480]	flagellated	no	79.472	no
[Ref.: #69480]	gram-positive	no	97.337	no
[Ref.: #69480]	anaerobic	no	93.599	yes
[Ref.: #69480]	halophile	no	72.406	no
[Ref.: #69480]	spore-forming	no	97.351	no
[Ref.: #69480]	glucose-util	yes	75.102	no
[Ref.: #69480]	aerobic	no	94.243	no
[Ref.: #69480]	thermophile	no	97.71	yes
[Ref.: #69480]	glucose-ferment	yes	73.15	no

Availability in culture collections External links

[Ref.: #23940]

Culture collection no.

DSM 104097, ATCC BAA-2448, NCIMB 14804

[Ref.: #90506]

SI-ID 397885

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Gilliamella intestini sp. nov., Gilliamella bombicola sp. nov., Gilliamella bombi sp. nov. and Gilliamella mensalis sp. nov.: Four novel Gilliamella species isolated from the bumblebee gut.	Praet J, Cnockaert M, Meeus I, Smagghe G, Vandamme P	Syst Appl Microbiol	10.1016/j.syapm.2017.03.003	2017	*
Phylogeny	Cultivation and characterization of the gut symbionts of honey bees and bumble bees: description of Snodgrassella alvi gen. nov., sp. nov., a member of the family Neisseriaceae of the Betaproteobacteria, and Gilliamella apicola gen. nov., sp. nov., a member of Orbaceae fam. nov., Orbales ord. nov., a sister taxon to the order 'Enterobacteriales' of the Gammaproteobacteria.	Kwong WK, Moran NA	Int J Syst Evol Microbiol	10.1099/ijs.0.044875-0	2012	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23940

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104097

#30807

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27138 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90506

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397885.1 )

#27138

IJSEM 2008 2013 ( DOI 10.1099/ijs.0.044875-0 , PubMed 23041637 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Gilliamella apicola in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF