Name: Stenotrophomonas indicatrix WS 40
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132060

Type strain:

Species: Stenotrophomonas indicatrix

Strain Designation: WS 40

Culture col. no.: DSM 28278, LMG 29942, CIP 111742, WS 40

Strain history: CIP <- 2019, LMG <- M. Weber, Friedrich Wilhelms Univ., Bonn, Germany <- W. Schünemann, Rheinische Friedrich Wilhelms Univ., Bonn, Germany: strain WS40

NCBI tax ID(s): 2045451 (species)

SI-ID 401787

DSM 28278

Section

Stenotrophomonas indicatrix WS 40 is an obligate aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from TSA-contact plate sample of dirty dishes.

colony-forming
Gram-negative
motile
rod-shaped
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Lysobacterales*
	Family	*Lysobacteraceae*
	Genus	*Stenotrophomonas*
	Species	**Stenotrophomonas indicatrix**
	Full Scientific Name (LPSN)	Stenotrophomonas indicatrix Weber et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65486]	Gram stain	negative
[Ref.: #120079]	Gram stain	negative

[Ref.: #65486]	Cell length	1.9-3.1 µm

[Ref.: #65486]	Cell width	0.8-1.2 µm

[Ref.: #65486]	Cell shape	rod-shaped
[Ref.: #120079]	Cell shape	rod-shaped

[Ref.: #65486]	Motility	yes
[Ref.: #120079]	Motility	yes

[Ref.: #65486]	Colony color	whitish yellow
[Ref.: #65486]	Colony shape	circular
[Ref.: #65486]	Cultivation medium used	Trypticase soy agar

Multimedia content

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

Tryptic Soy Agar

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Culture medium growth

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

CIP Medium 72

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #23851]		growth	28 °C
[Ref.: #65486]		growth	10-37 °C
[Ref.: #65486]	no	growth	40 °C

[Ref.: #23851]	Temperature range	mesophilic
[Ref.: #65486]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #65486]			growth	6-9
[Ref.: #65486]		no	growth	10

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

obligate aerobe

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65486]		NaCl		growth	4	%
[Ref.: #65486]		NaCl	no	growth	5	%

[Ref.: #65486]	Observation	Q-8 is the predominant ubiquinone
[Ref.: #65486]	Observation	Polar lipids are diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #65486]	D-fructose ChEBI	+	builds acid from
[Ref.: #65486]	D-glucose ChEBI	+	builds acid from
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #65486]	D-mannose ChEBI	+	builds acid from
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #65486]	D-xylose ChEBI	+	assimilation
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #65486]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #65486]	lactose ChEBI	+	assimilation
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #65486]	melibiose ChEBI	+	assimilation
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #65486]	potassium 2-dehydro-D-gluconate	+	assimilation
[Ref.: #65486]	salicin ChEBI	+	assimilation
[Ref.: #65486]	sucrose ChEBI	+	assimilation
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #65486]	turanose ChEBI	+	assimilation
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #65486]	valerate ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #65486]	catalase	+	1.11.1.6
[Ref.: #65486]	cytochrome oxidase	-	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65486]

Fatty acid	Percentage
C15:0 iso	54
C15:0 anteiso	32.5
C11:0 iso	4.2
C16:0	3.2
C16:1cis9	2
C14:0 iso	1.1
C16:0 iso	1
C14:0	0.9
C15:0	0.2
C17:0 iso	0.2
C13:0 iso 3OH	0.2
C12:0 3OH	0.1
C11:0 iso 3OH	0.1
C10:0	0.1

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #23851]	24957	+	-	-	-	-	+	+	+	+	-	+	-	+	+	-	-	-	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23851]	Sample type/isolated from	TSA-contact plate sample of dirty dishes
[Ref.: #23851]	Country	Germany
[Ref.: #23851]	Country ISO 3 Code	DEU
[Ref.: #23851]	Continent	Europe
[Ref.: #23851]	Geographic location	50.7469°/7.0967°

[Ref.: #65486]	Sample type/isolated from	dirty dishes originated from a study performed to identify indicator organisms for analysing the hygienic per- formance of dishwashers
[Ref.: #65486]	Country	Germany
[Ref.: #65486]	Country ISO 3 Code	DEU
[Ref.: #65486]	Continent	Europe
[Ref.: #65486]	Geographic location	50.7469°/7.0966°
[Ref.: #65486]	Enrichment culture duration	2 days
[Ref.: #65486]	Enrichment culture temperature	30 ̊C

[Ref.: #120079]	Sample type/isolated from	Dirty dishes
[Ref.: #120079]	Geographic location (country and/or sea, region)	Bonn
[Ref.: #120079]	Country	Germany
[Ref.: #120079]	Country ISO 3 Code	DEU
[Ref.: #120079]	Continent	Europe
[Ref.: #120079]	Isolation date	2013-03-01
* marker position based on {}

Isolation sources categories

#Engineered

#Industrial

#Engineered product

What are isolation sources categories?

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Global distribution of 16S sequence KJ452162 (>99% sequence identity) for Stenotrophomonas from Microbeatlas

Aquatic Samples	1647
Soil Samples	1650
Animal Samples	6387
Plant Samples	767
Total Samples	10451

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #23851]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120079]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65486]	16S rRNA gene sequences	KJ452162		GenBank

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GC-content

66.14 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #23851]

Culture collection no.

DSM 28278, LMG 29942, CIP 111742, WS 40

[Ref.: #90418]

SI-ID 401787

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Stenotrophomonas cyclobalanopsidis sp. nov., isolated from the leaf spot disease of Cyclobalanopsis patelliformis.	Bian DR, Xue H, Piao CG, Li Y	Antonie Van Leeuwenhoek	10.1007/s10482-020-01453-y	2020	*
Phylogeny	Stenotrophomonas lactitubi sp. nov. and Stenotrophomonas indicatrix sp. nov., isolated from surfaces with food contact.	Weber M, Schunemann W, Fuss J, Kampfer P, Lipski A	Int J Syst Evol Microbiol	10.1099/ijsem.0.002732	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23851

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28278

#65486

Mareike Weber, Wiebke Schünemann, Janina Fuß, Peter Kämpfer, André Lipski: Stenotrophomonas lactitubi sp. nov. and Stenotrophomonas indicatrix sp. nov., isolated from surfaces with food contact. IJSEM 68: 1830 - 1838 2018 ( DOI 10.1099/ijsem.0.002732 , PubMed 29638210 )

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#90418

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401787.1 )

#120079 Collection of Institut Pasteur ; Curators of the CIP; CIP 111742
* These data were automatically processed and therefore are not curated

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