Actinocrinis puniceicyclus (DSM 45618, ATCC BAA-2771, OB1)

Name: Actinocrinis puniceicyclus (DSM 45618, ATCC BAA-2771, OB1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132014

Type strain

Strain Designation OB1

Culture col. no. DSM 45618 ATCC BAA-2771 OB1

NCBI tax ID(s) 977794

Special Collections DSMZ Literature strains

Links

Actinocrinis puniceicyclus OB1 is an aerobe, spore-forming, Gram-positive prokaryote that forms circular colonies and builds aerial hyphae.

spore-forming Gram-positive filament-shaped colony-forming aerobe genome sequence 16S sequence

Name and taxonomic classification

SI-ID 405695

ATCC BAA-2771,DSM 45618

@ref 20215

Last LPSN update 2025-12-16 10:01:24

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Catenulisporales

Family Actinospicaceae

Genus Actinocrinis

Species Actinocrinis puniceicyclus

Full scientific name Actinocrinis puniceicyclus Kim et al. 2017

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
25215	positive	15-25 µm	1-1.5 µm	filament-shaped
125439	positive				99.9
125439					93.8

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
25215	0.5 mm	purple	circular	15 days	C10Y

Multicellular morphology

@ref	Forms multicellular complex	Complex name	Complex color	Medium name	Further description
25215		aerial hyphae	white	C10Y	older colonies turn white and produce extensive aerial hyphae

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
25215	C10Y		C10 supplemented with 0.05% (w/v) yeast extract, pH 5.0
25215	Yeast extract
25215	tryptic soy broth (Sigma)
25215	tryptone (Fisher Scientific)
23805	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
23805	positive	growth	20	psychrophilic
25215	positive	growth	13-30
25215	positive	optimum	20-25
25215	negative	growth	10
25215	negative	growth	37

Culture pH

@ref	Ability	Type	PH	PH range
25215	positive	optimum	5.6
25215	positive	growth	3.5-6.5	acidophile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
25215	aerobe
125439	obligate aerobe	98.6

Spore formation

@ref	Spore description	Type of spore	Spore formation
25215	chains of arthrospores	arthrospore

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
25215	30089 ChEBI	acetate	-	growth
25215	58187 ChEBI	alginate	+	growth
25215	16150 ChEBI	benzoate	-	growth
25215	62968 ChEBI	cellulose	-	growth
25215	17634 ChEBI	D-glucose	+	growth
25215	33403 ChEBI	elemental sulfur	-	growth
25215	16236 ChEBI	ethanol	-	growth
25215	29806 ChEBI	fumarate	-	growth
25215	28260 ChEBI	galactose	+	growth
25215	85248 ChEBI	gellan gum	+	growth
25215	29864 ChEBI	mannitol	+	growth
25215	37684 ChEBI	mannose	+	growth
25215	16183 ChEBI	methane	-	growth
25215	17790 ChEBI	methanol	-	growth
25215	17309 ChEBI	pectin	+	growth
25215	28831 ChEBI	propanol	-	growth
25215	15361 ChEBI	pyruvate	-	growth
25215	28017 ChEBI	starch	+	growth
25215	30031 ChEBI	succinate	-	growth
25215	17992 ChEBI	sucrose	+	growth
25215	37166 ChEBI	xylan	+	growth
25215	18222 ChEBI	xylose	+	growth

Enzymes

@ref	Value	Activity	Ec
25215	acid phosphatase	+	3.1.3.2
25215	alkaline phosphatase	+	3.1.3.1
25215	alpha-fucosidase	-	3.2.1.51
25215	alpha-galactosidase	+	3.2.1.22
25215	alpha-glucosidase	+	3.2.1.20
25215	alpha-mannosidase	-	3.2.1.24
25215	beta-galactosidase	+	3.2.1.23
25215	beta-glucosidase	-	3.2.1.21
25215	beta-glucuronidase	-	3.2.1.31
25215	cystine arylamidase	-	3.4.11.3
25215	esterase (C 4)	-
25215	esterase Lipase (C 8)	-
25215	leucine arylamidase	+	3.4.11.1
25215	lipase (C 14)	-
25215	N-acetyl-beta-glucosaminidase	+	3.2.1.52
25215	naphthol-AS-BI-phosphohydrolase	+
25215	trypsin	+	3.4.21.4
25215	valine arylamidase	+

Fatty acid profile

Metadata FA analysis

@ref

25215

Fatty acid	Percentage
C16:0 iso	28.5
C15:0 iso	14.6
C15:0 anteiso	14.3
C17:0 anteiso	9.5
C14:0 iso	6.9
C16:0	3.8
C17:0	3.8
C17:0 iso	2.6
iso-C17:1 ω8c	2
C17:1ω8c	1.9
anteiso-C17:1ω8c	1.7
C18:1 ω7c	1.6
C16:1ω7c	1.4
C18:0	1.2
iso-C16:1ω7c	1.2
C19:1	1.1
iso-C18:1ω9c	1
C15:0	0.8

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Wetland (Swamp)
#Condition	#Acidic	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture duration	Enrichment culture temperature
23805	acidic bog	Kootenay National Park, Ochre Beds bog	Canada	CAN	North America
25215	oxic, upper 4 cm layer of the Ochre Beds bog					salts medium C10	24 days	room temperature

Taxonmaps

69479

Global distribution of 16S sequence FR775984 (>99% sequence identity) for Actinocrinis puniceicyclus subclade from Microbeatlas

Aquatic Samples	493
Soil Samples	9654
Animal Samples	996
Plant Samples	1258
Total Samples	12401

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
23805	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID
66792	ASM1828364v1 assembly for Actinocrinis puniceicyclus DSM 45618	contig	GCA_018283645	977794.5	977794

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
23805	Actinobacterium OB1 partial 16S rRNA gene, isolate OB1	FR775984	1433		977794

GC content

@ref	GC-content (mol%)	Method
23805	70.2	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinocrinis puniceicyclus DSM 45618
NCBI: GCA_018283645

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	88.70	no
125439	motility	BacteriaNetⓘ	no	93.80	no
125439	gram_stain	BacteriaNetⓘ	positive	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinocrinis puniceicyclus strain DSM 45618
PATRIC: 977794.5

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	86.56	no
125438	anaerobic	anaerobicⓘ	no	94.73	yes
125438	aerobic	aerobicⓘ	yes	82.85	yes
125438	spore-forming	spore-formingⓘ	yes	84.86	no
125438	thermophilic	thermophileⓘ	no	90.92	yes
125438	flagellated	motile2+ⓘ	no	89.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinocrinis puniceicyclus gen. nov., sp. nov., an actinobacterium isolated from an acidic spring.	Kim JJ, Marjerrison CE, Cornish Shartau SL, Brady AL, Sharp CE, Rijpstra WIC, Sinninghe Damste JS, Schumann P, Grasby SE, Dunfield PF	Int J Syst Evol Microbiol	10.1099/ijsem.0.001667	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23805	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45618
#25215	J. J. M. Kim, C. E.,Cornish Shartau, S. L.,Brady, A. L.,Sharp, C. E.,Rijpstra, W. I.,Sinninghe Damsté, J. S.,Schumann, P.,Grasby, S. E.,Dunfield, P. F.: Actinocrinis puniceicyclus gen. nov., sp. nov., an actinobacterium isolated from an acidic spring. IJSEM 67: 602 - 609 2017 ( DOI 10.1099/ijsem.0.001667 , PubMed 27902294 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinocrinis puniceicyclus (DSM 45618, ATCC BAA-2771, OB1)

Name and taxonomic classification