Name: Pseudomonas saponiphila 102 G
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 13187

Type strain:

Species: Pseudomonas saponiphila

Strain Designation: 102 G, 102G

Culture col. no.: DSM 9751, CIP 109856

Strain history: CIP <- 2008, DSMZ <- W.F. Guerin, Michingan State Univ., Michigan, USA: strain 102G

NCBI tax ID(s): 556534 (species)

SI-ID 48690

DSM 9751, CIP 109856

Special Collections

DSMZ
CIP

Section

When using BacDive for research please cite our paper

Pseudomonas saponiphila 102 G is an obligate aerobe, mesophilic, Gram-negative bacterium of the family Pseudomonadaceae.

Gram-negative
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas saponiphila**
	Full Scientific Name (LPSN)	Pseudomonas saponiphila Lang et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #122486]	Gram stain	negative
[Ref.: #125438]	Gram stain	negative Confidence in %96.982
[Ref.: #125439]	Gram stain	negative Confidence in %98.3

[Ref.: #122486]	Cell shape	rod-shaped

[Ref.: #122486]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3713]

Culture medium

REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)

[Ref.: #3713]

Culture medium growth

[Ref.: #3713]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3713]

Culture medium composition

expand / minimize

[Ref.: #3713]

Culture medium

MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)

[Ref.: #3713]

Culture medium growth

[Ref.: #3713]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3713]

Culture medium composition

expand / minimize

[Ref.: #37363]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37363]

Culture medium growth

[Ref.: #37363]

Culture medium composition

expand / minimize

[Ref.: #122486]

Culture medium

CIP Medium 72

[Ref.: #122486]

Culture medium growth

[Ref.: #122486]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #3713]			growth	28 °C
[Ref.: #37363]			growth	30 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #122486]	Oxygen tolerance	obligate aerobe
[Ref.: #125438]	Oxygen tolerance	aerobe Confidence in %91.193

[Ref.: #125439]

Ability of spore formation

no Confidence in %98.7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #122486]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #122486]	nitrite ChEBI	-	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #122486]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122486]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122486]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #122486]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #3713]	25526	-	-	-	+	-	-	+	-	+	-	+	+	+	-	+	+	-	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122486]	12164	-	+	+	+	-	+	+	-	+	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3713]	Geographic location (country and/or sea, region)	Michigan
[Ref.: #3713]	Country	USA
[Ref.: #3713]	Country ISO 3 Code	USA
[Ref.: #3713]	Continent	North America

[Ref.: #122486]	Geographic location (country and/or sea, region)	Michigan
[Ref.: #122486]	Country	United States of America
[Ref.: #122486]	Country ISO 3 Code	USA
[Ref.: #122486]	Continent	North America
[Ref.: #122486]	Isolation date	1995
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3713]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122486]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #3713]	Pseudomonas saponiphila 16S rRNA gene, type strain DSM 9751T	FM208264	1530	GenBank ENA	556534 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas saponiphila DSM 9751	GCA_900105185	contig	GenBank ENA	556534 tax ID	*
[Ref.: #66792]	Pseudomonas saponiphila strain DSM 9751	556534.4	wgs	PATRIC	556534 tax ID	*
[Ref.: #66792]	Pseudomonas saponiphila DSM 9751	2667528183	draft	JGI IMG/M	556534 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudomonas saponiphila strain DSM 9751 PATRIC: 556534.4
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	96.982	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	97.562	yes
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	85.34	no
[Ref.: #125438]	aerobic	aerobicⓘ	yes	91.193	yes
[Ref.: #125438]	thermophile	thermophilicⓘ	no	97.997	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	yes	84.109	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Pseudomonas saponiphila DSM 9751 NCBI: GCA_900105185
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	98.7
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	89.1
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	98.3
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	80.4

Availability in culture collections External links

[Ref.: #3713]

Culture collection no.

DSM 9751, CIP 109856

[Ref.: #82395]

SI-ID 48690

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas benzenivorans sp. nov. and Pseudomonas saponiphila sp. nov., represented by xenobiotics degrading type strains.	Lang E, Burghartz M, Spring S, Swiderski J, Sproer C	Curr Microbiol	10.1007/s00284-009-9507-7	2009	*

References

#3713

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 9751

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#37363 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#82395

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID48690.1 )

#122486 Collection of Institut Pasteur ; Curators of the CIP; CIP 109856

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

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Pseudomonas saponiphila strain DSM 9751

Pseudomonas saponiphila DSM 9751

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