Name: Nocardiopsis alkaliphila WS 2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 131849

Type strain:

Species: Nocardiopsis alkaliphila

Strain Designation: WS 2

Culture col. no.: DSM 44657, CCTCC AA 001031, JCM 12678, YIM 80379, NBRC 104163

Strain history: DSM 44657 <-- W. N. Hozzein WS2 (=YIM 80379).

NCBI tax ID(s): 1246461 (strain), 225762 (species)

SI-ID 97671

DSM 44657, JCM 12678, NBRC 104163

Special Collections

Section

Nocardiopsis alkaliphila WS 2 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from soil.

Gram-positive
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Nocardiopsidaceae*
	Genus	*Nocardiopsis*
	Species	**Nocardiopsis alkaliphila**
	Full Scientific Name (LPSN)	Nocardiopsis alkaliphila Hozzein et al. 2004

Information on morphological and physiological properties Morphology

[Ref.: #30015]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %90.257

[Ref.: #30015]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %92.5

[Ref.: #30015]

Pigment production

yes

Multimedia content

[Ref.: #11966]

Caption:

Medium 215 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

CZAPEK PEPTONE AGAR (DSMZ Medium 83)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

BHI MEDIUM (DSMZ Medium 215)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #11966]	growth	28 °C
[Ref.: #30015]	growth	10-45 °C
[Ref.: #30015]	optimum	28-30 °C
[Ref.: #67770]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #30015]			optimum	9.5-10.0
[Ref.: #30015]			growth	7.0-12.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30015]	Oxygen tolerance	aerobe
[Ref.: #69480]	Oxygen tolerance	aerobe Confidence in %90.151

[Ref.: #30015]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30015]		NaCl		growth	0-10	%
[Ref.: #30015]		NaCl		optimum	2.5	%

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Observation

quinones: MK-10(H₆), MK-10(H₈), MK-11(H₂), MK-9(H₆), MK-9(H₁₀), MK-10(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30015]	arabinose ChEBI	+	carbon source
[Ref.: #30015]	cellobiose ChEBI	+	carbon source
[Ref.: #30015]	galactose ChEBI	+	carbon source
[Ref.: #30015]	glucose ChEBI	+	carbon source
[Ref.: #30015]	lactose ChEBI	+	carbon source
[Ref.: #30015]	maltose ChEBI	+	carbon source
[Ref.: #30015]	raffinose ChEBI	+	carbon source
[Ref.: #30015]	rhamnose ChEBI	+	carbon source
[Ref.: #30015]	serine ChEBI	+	carbon source
[Ref.: #30015]	sorbitol ChEBI	+	carbon source
[Ref.: #30015]	sucrose ChEBI	+	carbon source
[Ref.: #30015]	xylitol ChEBI	+	carbon source
[Ref.: #30015]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #30015]		catalase	+	1.11.1.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11966]	Sample type/isolated from	soil
[Ref.: #11966]	Geographic location (country and/or sea, region)	The eastern desert
[Ref.: #11966]	Country	Egypt
[Ref.: #11966]	Country ISO 3 Code	EGY
[Ref.: #11966]	Continent	Africa

[Ref.: #67770]	Sample type/isolated from	Soil, the eastern desert of Egypt
[Ref.: #67770]	Country	Egypt
[Ref.: #67770]	Country ISO 3 Code	EGY
[Ref.: #67770]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11966]	Nocardiopsis egyptensis 16S ribosomal RNA gene, partial sequence	AY230848	1516	GenBank ENA	225762 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Nocardiopsis alkaliphila YIM 80379	GCA_000341005	contig	GenBank ENA	1246461 tax ID
[Ref.: #66792]	Nocardiopsis alkaliphila YIM 80379	1246461.3	wgs	PATRIC	1246461 tax ID	*
[Ref.: #66792]	Nocardiopsis alkaliphila YIM 80379	2551306677	draft	JGI IMG/M	1246461 tax ID	*

[Ref.: #11966]	GC-content	65.8 mol%
[Ref.: #67770]	GC-content	67.5 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	65.8 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nocardiopsis alkaliphila YIM 80379 PATRIC: 1246461.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Nocardiopsis alkaliphila YIM 80379 PATRIC: 1246461.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	90.257	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	97.35	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	79.735	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	90.151	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.763	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	92.5	yes

Availability in culture collections External links

[Ref.: #11966]

Culture collection no.

DSM 44657, CCTCC AA 001031, JCM 12678, YIM 80379, NBRC 104163

[Ref.: #90240]

SI-ID 97671

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nocardiopsis alkaliphila sp. nov., a novel alkaliphilic actinomycete isolated from desert soil in Egypt.	Hozzein WN, Li WJ, Ali MIA, Hammouda O, Mousa AS, Xu LH, Jiang CL	Int J Syst Evol Microbiol	10.1099/ijs.0.02832-0	2004	*

References

#11966

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44657

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30015

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26379 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90240

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID97671.1 )

#26379

IJSEM 247 2004 ( DOI 10.1099/ijs.0.02832-0 , PubMed 14742488 )

* These data were automatically processed and therefore are not curated

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Nocardiopsis alkaliphila YIM 80379

Nocardiopsis alkaliphila YIM 80379

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