Name: Mycobacterium houstonense MF-89
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 131823

Type strain:

Species: Mycobacterium houstonense

Strain Designation: MF-89, W5198

Culture col. no.: DSM 44676, ATCC 49403, CDC 80-719, CCUG 47581, JCM 15656, CIP 107828

Strain history: CIP <- 2003, G. Goolsby, CDC, Atlanta, USA: strain W5198 <- 1997, R.J. Wallace, Univ. Texas Hlth. Cent., Tyler, Texas, USA: strain MF 89, Mycobacterium fortuitum

NCBI tax ID(s): 146021 (species)

SI-ID 42454

ATCC 49403, CIP 105353, DSM 44676, CIP 107828, JCM 15656

Section

Mycobacterium houstonense MF-89 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from facial abscess.

Gram-positive
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium houstonense**
	Full Scientific Name (LPSN)	Mycobacterium houstonense Schinsky et al. 2004

Synonym

Mycolicibacterium houstonense

Information on morphological and physiological properties Morphology

[Ref.: #29958]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #119977]	Cell shape	rod-shaped

[Ref.: #119977]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %91.529

Multimedia content

[Ref.: #11973]

Caption:

Medium 645 37°C

[Ref.: #11973]

Intellectual property rights:

[Ref.: #11973]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11973]

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

[Ref.: #11973]

Culture medium growth

[Ref.: #11973]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11973]

Culture medium composition

expand / minimize

[Ref.: #36379]

Culture medium

MEDIUM 55 - for Mycobacterium

[Ref.: #36379]

Culture medium growth

[Ref.: #119977]

Culture medium

CIP Medium 55

[Ref.: #119977]

Culture medium growth

[Ref.: #119977]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #119977]

Culture medium

CIP Medium 72

[Ref.: #119977]

Culture medium growth

[Ref.: #119977]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #11973]		growth	37 °C
[Ref.: #29958]		growth	28-42 °C
[Ref.: #29958]		optimum	35 °C
[Ref.: #36379]		growth	37 °C
[Ref.: #57764]		growth	37-42 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #119977]		growth	22-37 °C
[Ref.: #119977]	no	growth	10 °C
[Ref.: #119977]	no	growth	45 °C

[Ref.: #11973]	Temperature range	mesophilic
[Ref.: #29958]	Temperature range	mesophilic
[Ref.: #36379]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #119977]	Temperature range	psychrophilic
[Ref.: #119977]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #57764]	Oxygen tolerance	aerobe
[Ref.: #119977]	Oxygen tolerance	obligate aerobe

[Ref.: #29958]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %98
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.941

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29958]		NaCl		optimum	5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29958]	fructose ChEBI	+	carbon source
[Ref.: #29958]	glucose ChEBI	+	carbon source
[Ref.: #119977]	hippurate ChEBI	-	hydrolysis
[Ref.: #29958]	mannitol ChEBI	+	carbon source
[Ref.: #29958]	mannose ChEBI	+	carbon source
[Ref.: #29958]	myo-inositol ChEBI	+	carbon source
[Ref.: #29958]	nitrate ChEBI	+	reduction
[Ref.: #119977]	nitrate ChEBI	+	reduction
[Ref.: #119977]	nitrate ChEBI	-	respiration
[Ref.: #119977]	nitrite ChEBI	-	reduction
[Ref.: #29958]	sorbitol ChEBI	+	carbon source
[Ref.: #29958]	trehalose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #119977]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #119977]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #119977]	amylase	-
[Ref.: #29958]	arylsulfatase	+	3.1.6.1
[Ref.: #119977]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119977]	caseinase	-	3.4.21.50
[Ref.: #29958]	catalase	+	1.11.1.6
[Ref.: #119977]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #119977]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #119977]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #119977]	gelatinase	-
[Ref.: #119977]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #119977]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119977]	oxidase	-
[Ref.: #119977]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119977]	tween esterase	+
[Ref.: #29958]	urease	+	3.5.1.5
[Ref.: #119977]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #119977]	2457	not determinedn.d.	+	+	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	+	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	-	+	-	+	-	-	+	-	-	+	+	-	-	-	+	-	-	-	+	+	+	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119977]	10682	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11973]	Sample type/isolated from	facial abscess
[Ref.: #11973]	Geographic location (country and/or sea, region)	Texas, Houston
[Ref.: #11973]	Country	USA
[Ref.: #11973]	Country ISO 3 Code	USA
[Ref.: #11973]	Continent	North America

[Ref.: #57764]	Sample type/isolated from	Human facial abscess
[Ref.: #57764]	Geographic location (country and/or sea, region)	Houston
[Ref.: #57764]	Country	USA
[Ref.: #57764]	Country ISO 3 Code	USA
[Ref.: #57764]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Face wound
[Ref.: #67770]	Geographic location (country and/or sea, region)	Houston, TX
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America

[Ref.: #119977]	Sample type/isolated from	Face wound
[Ref.: #119977]	Geographic location (country and/or sea, region)	Houston, Texas
[Ref.: #119977]	Country	United States of America
[Ref.: #119977]	Country ISO 3 Code	USA
[Ref.: #119977]	Continent	North America
[Ref.: #119977]	Isolation date	1980
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Other	#Abscess
#Host Body-Site	#Organ	#Skin, Nail, Hair

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LT223693 (>99% sequence identity) for Mycolicibacterium from Microbeatlas

Aquatic Samples	64
Soil Samples	45
Animal Samples	50
Plant Samples	337
Total Samples	496

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11973]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #119977]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11973]	Mycobacterium houstonense strain Ho1as 16S ribosomal RNA gene, partial sequence	AY012579	1440	ENA	146021 tax ID
[Ref.: #67770]	Mycobacterium houstonense partial 16S rRNA gene, strain ATCC 49403	LT223693	1506	ENA	146021 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycolicibacterium houstonense ATCC 49403T	GCA_900078665	scaffold	GenBank	146021 tax ID
[Ref.: #66792]	Mycobacterium houstonense strain type strain: ATCC 49403	146021.3	wgs	PATRIC	146021 tax ID	*
[Ref.: #66792]	Mycolicibacterium houstonense ATCC 49403	2775507220	draft	JGI IMG/M	146021 tax ID	*
[Ref.: #66792]	Mycolicibacterium houstonense DSM 44676	GCA_025823315	scaffold	GenBank	146021 tax ID	*

[Ref.: #11973]	GC-content	64 mol%
[Ref.: #67770]	GC-content	64 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	67 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Mycolicibacterium houstonense ATCC 49403T NCBI: GCA_900078665.2
[Ref.: #69481]	spore-forming	no	98	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacterium houstonense strain type strain: ATCC 49403 PATRIC: 146021.3
[Ref.: #69480]	motile	no	93.209	no
[Ref.: #69480]	gram-positive	yes	90.836	no
[Ref.: #69480]	anaerobic	no	99.527	no
[Ref.: #69480]	halophile	no	92.585	no
[Ref.: #69480]	spore-forming	no	86.712	yes
[Ref.: #69480]	thermophile	no	99.551	yes
[Ref.: #69480]	glucose-util	yes	88.157	yes
[Ref.: #69480]	aerobic	yes	89.399	yes
[Ref.: #69480]	flagellated	no	97.959	no
[Ref.: #69480]	glucose-ferment	no	90.337	no

Availability in culture collections External links

[Ref.: #11973]

Culture collection no.

DSM 44676, ATCC 49403, CDC 80-719, CCUG 47581, JCM 15656, CIP 107828

[Ref.: #90214]

SI-ID 42454

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp. nov., Mycobacterium houstonense sp. nov., Mycobacterium neworleansense sp. nov. and Mycobacterium brisbanense sp. nov. and recognition of Mycobacterium porcinum from human clinical isolates.	Schinsky MF, Morey RE, Steigerwalt AG, Douglas MP, Wilson RW, Floyd MM, Butler WR, Daneshvar MI, Brown-Elliott BA, Wallace RJ, McNeil MM, Brenner DJ, Brown JM	Int J Syst Evol Microbiol	10.1099/ijs.0.02743-0	2004	*
Genetics	Draft Genome Sequence of Mycobacterium houstonense Strain ATCC 49403T.	Levasseur A, Asmar S, Robert C, Drancourt M	Genome Announc	10.1128/genomeA.00443-16	2016	*

References

#11973

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44676

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29958

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26327 (see below)

#36379 ; Curators of the CIP;
#57764 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47581

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90214

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID42454.1 )

#119977 Collection of Institut Pasteur ; Curators of the CIP; CIP 107828

#26327

IJSEM 1653 2004 ( DOI 10.1099/ijs.0.02743-0 , PubMed 15388725 )

* These data were automatically processed and therefore are not curated

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Mycolicibacterium houstonense ATCC 49403T

Mycobacterium houstonense strain type strain: ATCC 49403

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