Mycobacterium houstonense (DSM 44676, ATCC 49403, CDC 80-719)

Name: Mycobacterium houstonense (DSM 44676, ATCC 49403, CDC 80-719)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 131823

Type strain

Strain Designation MF-89, W5198

Culture col. no. DSM 44676 ATCC 49403 CDC 80-719 CCUG 47581 MF-89 3 more

NCBI tax ID(s) 146021

Special Collections DSMZ CCUG JCM CIP Literature strains

History DSMZ <- J. M. Brown, CDC <- R. J. Wallace JCM <-- CIP 107828 <-- G. Goolsby W5198 <-- R. J. Wallace Mf-89. CIP <- 2003, G. Goolsby, CDC, Atlanta, USA: strain W5198 <- 1997, R.J. Wallace, Univ. Texas Hlth. Cent., Tyler, Texas, USA: strain MF 89, Mycobacterium fortuitum

Links

Mycobacterium houstonense MF-89 is an aerobe, Gram-positive bacterium that was isolated from facial abscess.

Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 42454

ATCC 49403,CIP 105353,DSM 44676,CIP 107828,JCM 15656,CCUG 47581T

@ref 20215

Last LPSN update 2026-05-29 08:37:42

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Mycobacteriaceae

Genus Mycobacterium

Species Mycobacterium houstonense

Full scientific name Mycobacterium houstonense Schinsky et al. 2004

Synonyms (1)

Mycolicibacterium houstonense

Morphology

Cell morphology

@ref	Confidence	Gram stain	Cell shape
29958		positive
125438	94.5
125438	93.155	positive
119977			rod-shaped

Multimedia

@ref:	11973
multimedia content:	DSM_44676.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44676.jpg
caption:	Medium 645 37°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

@ref:	11973
multimedia content:	DSM_44676-1.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44676-1.jpg
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11973	MIDDLEBROOK MEDIUM (DSMZ Medium 645)	Medium recipe at MediaDive	Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
36379	MEDIUM 55 - for Mycobacterium
119977	CIP Medium 55	Medium recipe at CIP
119977	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
11973	positive	growth	37
29958	positive	growth	28-42
29958	positive	optimum	35
36379	positive	growth	37
57764	positive	growth	37-42
67770	positive	growth	37
119977	positive	growth	22-37
119977	negative	growth	10
119977	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
57764	aerobe
119977	obligate aerobe
125439	obligate aerobe	95.557

Spore formation

29958

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29958	NaCl	positive	optimum	5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29958	28757 ChEBI	fructose	+	carbon source
29958	17234 ChEBI	glucose	+	carbon source
119977	606565 ChEBI	hippurate	-	hydrolysis
29958	29864 ChEBI	mannitol	+	carbon source
29958	37684 ChEBI	mannose	+	carbon source
29958	17268 ChEBI	myo-inositol	+	carbon source
29958	17632 ChEBI	nitrate	+	reduction
119977	17632 ChEBI	nitrate	+	reduction
119977	17632 ChEBI	nitrate	-	respiration
119977	16301 ChEBI	nitrite	-	reduction
29958	30911 ChEBI	sorbitol	+	carbon source
29958	27082 ChEBI	trehalose	+	carbon source

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
119977	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119977	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
119977	amylase	-
29958	arylsulfatase	+	3.1.6.1
68382	beta-galactosidase	-	3.2.1.23	from API zym
119977	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119977	caseinase	-	3.4.21.50
29958	catalase	+	1.11.1.6
119977	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
119977	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
119977	gamma-glutamyltransferase	+	2.3.2.2
119977	gelatinase	-
119977	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
119977	lipase	-
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119977	oxidase	-
119977	protease	-
68382	trypsin	-	3.4.21.4	from API zym
119977	tween esterase	+
29958	urease	+	3.5.1.5
119977	urease	+	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119977	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	-	+	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
119977	not determinedn.d.	+	+	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	+	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	-	+	-	+	-	-	+	-	-	+	+	-	-	-	+	-	-	-	+	+	+	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host Body-Site	#Other	#Abscess
#Host Body-Site	#Organ	#Skin, Nail, Hair

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
11973	facial abscess		Texas, Houston	USA	USA	North America
57764	Human facial abscess	Homo sapiens	Houston	USA	USA	North America
67770	Face wound		Houston, TX	USA	USA	North America
119977	Face wound		Houston, Texas	United States of America	USA	North America	1980

Taxonmaps

69479

Global distribution of 16S sequence LT223693 (>99% sequence identity) for Mycolicibacterium from Microbeatlas

Aquatic Samples	64
Soil Samples	45
Animal Samples	50
Plant Samples	337
Total Samples	496

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11973	2	Risk group (German classification) According to TRBA 466
119977	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2582331v1 assembly for Mycolicibacterium houstonense DSM 44676	scaffold	GCA_025823315	146021.5	8047413270	146021	74.43
66792	Mycobacterium houstonense strain type strain: ATCC 49403	contig		146021.3		146021	33.6
67770	PRJEB13221 assembly for Mycolicibacterium houstonense type strain: ATCC 49403	scaffold	GCA_900078665	146021.6	2775507220	146021	30.45

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
11973	Mycobacterium houstonense strain Ho1as 16S ribosomal RNA gene, partial sequence	AY012579	1440	146021
67770	Mycobacterium houstonense partial 16S rRNA gene, strain ATCC 49403	LT223693	1506	146021
124043	Rhodococcus sp. 5-11 16S ribosomal RNA gene, partial sequence.	AY457067	1483	146021
124043	Mycobacterium houstonense strain DSM 44676 16S ribosomal RNA gene, partial sequence.	DQ987743	541	133549
124043	Mycobacterium houstonense strain DSM 44676 16S ribosomal RNA gene, partial sequence.	DQ987744	529	146021

GC content

@ref	GC-content (mol%)	Method
11973	64
67770	64	thermal denaturation, midpoint method (Tm)
67770	67	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM2582331v1 assembly for Mycolicibacterium houstonense DSM 44676
NCBI: GCA_025823315

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	95.56	no
125439	gram_stain	BacteriaNetⓘ	positive	79.94	no
125439	spore_formation	BacteriaNetⓘ	no	84.84	no
125439	motility	BacteriaNetⓘ	no	73.83	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Mycobacterium houstonense strain type strain: ATCC 49403
PATRIC: 146021.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	93.16	yes
125438	anaerobic	anaerobicⓘ	no	98.06	yes
125438	spore-forming	spore-formingⓘ	no	68.73	yes
125438	aerobic	aerobicⓘ	yes	88.33	yes
125438	thermophilic	thermophileⓘ	no	98.50	yes
125438	flagellated	motile2+ⓘ	no	94.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera.	Gupta RS, Lo B, Son J.	Front Microbiol	10.3389/fmicb.2018.00067	2018
Enzymology	Evaluation of the invader assay with the BACTEC MGIT 960 system for prompt isolation and identification of mycobacterial species from clinical specimens.	Ichimura S, Nagano M, Ito N, Shimojima M, Egashira T, Miyamoto C, Ohkusu K, Ezaki T.	J Clin Microbiol	10.1128/jcm.02289-06	2007
Genetics	Draft Genome Sequence of Mycobacterium houstonense Strain ATCC 49403T.	Levasseur A, Asmar S, Robert C, Drancourt M	Genome Announc	10.1128/genomeA.00443-16	2016
Phylogeny	Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp. nov., Mycobacterium houstonense sp. nov., Mycobacterium neworleansense sp. nov. and Mycobacterium brisbanense sp. nov. and recognition of Mycobacterium porcinum from human clinical isolates.	Schinsky MF, Morey RE, Steigerwalt AG, Douglas MP, Wilson RW, Floyd MM, Butler WR, Daneshvar MI, Brown-Elliott BA, Wallace RJ, McNeil MM, Brenner DJ, Brown JM	Int J Syst Evol Microbiol	10.1099/ijs.0.02743-0	2004

References

#11973	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44676
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26327	IJSEM 1653 2004 ( DOI 10.1099/ijs.0.02743-0 , PubMed 15388725 )
#29958	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26327
#36379	; Curators of the CIP;
#57764	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47581
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119977	Collection of Institut Pasteur ; Curators of the CIP; CIP 107828
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mycobacterium houstonense (DSM 44676, ATCC 49403, CDC 80-719)

Name and taxonomic classification

Morphology

Cell morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_025823315

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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