Name: Pseudomonas oleovorans subsp. lubricantis RS1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13179

Type strain:

Species: Pseudomonas oleovorans subsp. lubricantis

Strain Designation: RS1

Culture col. no.: DSM 21016, ATCC BAA-1494

Strain history: <- R. Saha, Michigan Technological University; RS1

NCBI tax ID(s): 398198 (subspecies)

SI-ID 404012

DSM 21016

Section

Pseudomonas oleovorans subsp. lubricantis RS1 is a mesophilic bacterium that was isolated from contaminated metalworking fluids.

mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas oleovorans subsp. lubricantis**
	Full Scientific Name (LPSN)	Pseudomonas oleovorans subsp. lubricantis Saha et al. 2010

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15511]

Culture medium

TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #15511]			growth	37 °C

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Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #15511]	22899	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15511]	Sample type/isolated from	contaminated metalworking fluids
[Ref.: #15511]	Geographic location (country and/or sea, region)	Houghton, Michigan
[Ref.: #15511]	Country	USA
[Ref.: #15511]	Country ISO 3 Code	USA
[Ref.: #15511]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered	#Contamination	-
#Engineered	#Industrial	#Industrial production

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15511]	Pseudomonas oleovorans subsp. lubricantis strain RS1 DNA gyrase subunit B (gyrB) gene, partial cds	EF675630	1171	ENA	398198 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas oleovorans RS1	GCA_900109155	scaffold	GenBank	301 tax ID	*

[Ref.: #15511]

GC-content

62.2 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas oleovorans RS1 NCBI: GCA_900109155.1
[Ref.: #69480]	glucose-ferment	no	91.738	yes
[Ref.: #69480]	motile	yes	88.112	no
[Ref.: #69480]	flagellated	yes	88.663	no
[Ref.: #69480]	gram-positive	no	98.75	no
[Ref.: #69480]	anaerobic	no	98.411	no
[Ref.: #69480]	aerobic	yes	89.28	no
[Ref.: #69480]	halophile	no	76.754	no
[Ref.: #69480]	spore-forming	no	96.48	no
[Ref.: #69480]	thermophile	no	99.447	no
[Ref.: #69480]	glucose-util	yes	89.817	no

Availability in culture collections External links

[Ref.: #15511]

Culture collection no.

DSM 21016, ATCC BAA-1494

[Ref.: #82387]

SI-ID 404012

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Taxonomic description and draft genome of Pseudomonas sediminis sp. nov., isolated from the rhizospheric sediment of Phragmites karka.	Behera P, Mahapatra M, Seuylemezian A, Vaishampayan P, Ramana VV, Joseph N, Joshi A, Shouche Y, Suar M, Pattnaik AK, Rastogi G	J Microbiol	10.1007/s12275-018-7549-x	2018	*
Phylogeny	Pseudomonas zhaodongensis sp. nov., isolated from saline and alkaline soils.	Zhang L, Pan Y, Wang K, Zhang X, Zhang C, Zhang S, Fu X, Jiang J	Int J Syst Evol Microbiol	10.1099/ijs.0.000057	2015	*
Phylogeny	Pseudomonas guguanensis sp. nov., a gammaproteobacterium isolated from a hot spring.	Liu YC, Young LS, Lin SY, Hameed A, Hsu YH, Lai WA, Shen FT, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.047712-0	2013	*
Phylogeny	Pseudomonas oleovorans subsp. lubricantis subsp. nov., and reclassification of Pseudomonas pseudoalcaligenes ATCC 17440T as later synonym of Pseudomonas oleovorans ATCC 8062 T.	Saha R, Sproer C, Beck B, Bagley S	Curr Microbiol	10.1007/s00284-009-9540-6	2009	*

References

#15511

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21016

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#82387

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404012.1 )

* These data were automatically processed and therefore are not curated

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Pseudomonas oleovorans RS1

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