Name: Pseudomonas extremaustralis 14-3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13177

Type strain:

Species: Pseudomonas extremaustralis

Strain Designation: 14-3, BM, CT14-3, CT 14-3

Culture col. no.: DSM 17835, CIP 109839, CCM 7898

Strain history: CIP <- 2008, DSMZ <- B. Mendez, Buenos Aires Univ., Buenos Aires, Argentina: strain: CT 14-3

NCBI tax ID(s): 359110 (species)

SI-ID 368972

CIP 109839, DSM 17835, CCM 7898, LMG 28988

Other strains from Pseudomonas extremaustralis

P. extremaustralis 14-3b, DSM 25547

Section

Pseudomonas extremaustralis 14-3 is an obligate aerobe, mesophilic, motile bacterium that was isolated from temporary water pond.

motile
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas extremaustralis**
	Full Scientific Name (LPSN)	Pseudomonas extremaustralis López et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #119157]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #119157]	Cell shape	rod-shaped

[Ref.: #119157]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %95.123

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7206]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #7206]

Culture medium growth

[Ref.: #7206]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7206]

Culture medium composition

expand / minimize

[Ref.: #7206]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #7206]

Culture medium growth

[Ref.: #7206]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7206]

Culture medium composition

expand / minimize

[Ref.: #37664]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37664]

Culture medium growth

[Ref.: #37664]

Culture medium composition

expand / minimize

[Ref.: #119157]

Culture medium

CIP Medium 72

[Ref.: #119157]

Culture medium growth

[Ref.: #119157]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #7206]			growth	30 °C
[Ref.: #37664]			growth	30 °C

[Ref.: #7206]	Temperature range	mesophilic
[Ref.: #37664]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #119157]

Oxygen tolerance

obligate aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.977

[Ref.: #7206]

Name of produced compound

poly(ß-hydroxybutyric acid)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #119157]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #119157]	nitrite ChEBI	-	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #119157]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119157]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119157]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #119157]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #7206]	23012	+	-	-	+	-	-	-	-	+	+	+	+	+	-	+	+	+	+	+	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119157]	12150	-	+	-	+	+	+	-	-	+	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7206]	Sample type/isolated from	temporary water pond
[Ref.: #7206]	Geographic location (country and/or sea, region)	Cierva Point, Danco Coast
[Ref.: #7206]	Continent	Australia and Oceania

[Ref.: #119157]	Sample type/isolated from	Environment, Water pond
[Ref.: #119157]	Country	Antarctica
[Ref.: #119157]	Country ISO 3 Code	ATA
[Ref.: #119157]	Continent	Antarctica
[Ref.: #119157]	Isolation date	1994
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Pond (small)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ583501 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	976
Soil Samples	555
Animal Samples	2173
Plant Samples	1759
Total Samples	5463

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7206]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119157]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7206]	Pseudomonas extremaustralis 16S rRNA gene, strain CT14-3	AJ583501	1505	ENA	1117955 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas extremaustralis DSM 17835	GCA_900102035	chromosome	GenBank	359110 tax ID	*
[Ref.: #66792]	Pseudomonas extremaustralis DSM 17835	GCA_007858235	contig	GenBank	359110 tax ID	*
[Ref.: #66792]	Pseudomonas extremaustralis strain DSM 17835	359110.16	wgs	PATRIC	359110 tax ID	*
[Ref.: #66792]	Pseudomonas extremaustralis strain DSM 17835	359110.7	complete	PATRIC	359110 tax ID	*
[Ref.: #66792]	Pseudomonas extremaustralis DSM 17835	2667527225	draft	JGI IMG/M	359110 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pseudomonas extremaustralis strain DSM 17835 PATRIC: 359110.7
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas extremaustralis strain DSM 17835 PATRIC: 359110.7
[Ref.: #69480]	gram-positive	no	98.339	no
[Ref.: #69480]	anaerobic	no	97.903	no
[Ref.: #69480]	halophile	no	89.784	no
[Ref.: #69480]	spore-forming	no	96.171	no
[Ref.: #69480]	glucose-util	yes	94.85	yes
[Ref.: #69480]	flagellated	yes	83.05	no
[Ref.: #69480]	aerobic	yes	88.349	no
[Ref.: #69480]	motile	yes	88.789	no
[Ref.: #69480]	thermophile	no	99.78	yes
[Ref.: #69480]	glucose-ferment	no	89.528	no

Availability in culture collections External links

[Ref.: #7206]

Culture collection no.

DSM 17835, CIP 109839, CCM 7898

[Ref.: #82385]

SI-ID 368972

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas petroselini sp. nov., a pathogen causing bacterial rot of parsley in Japan.	Sawada H, Fujikawa T, Osada S, Satou M	Int J Syst Evol Microbiol	10.1099/ijsem.0.005424	2022	*
Phylogeny	Pseudomonas cyclaminis sp. nov., a pathogen causing bacterial bud blight of cyclamen in Japan.	Sawada H, Fujikawa T, Osada S, Satou M	Int J Syst Evol Microbiol	10.1099/ijsem.0.004723	2021	*
Phylogeny	Pseudomonas kitaguniensis sp. nov., a pathogen causing bacterial rot of Welsh onion in Japan.	Sawada H, Fujikawa T, Nishiwaki Y, Horita H	Int J Syst Evol Microbiol	10.1099/ijsem.0.004123	2020	*
Genetics	Draft genome sequence of Pseudomonas extremaustralis strain USBA-GBX 515 isolated from Superparamo soil samples in Colombian Andes.	Lopez G, Diaz-Cardenas C, Shapiro N, Woyke T, Kyrpides NC, David Alzate J, Gonzalez LN, Restrepo S, Baena S	Stand Genomic Sci	10.1186/s40793-017-0292-9	2017	*
Phylogeny	Pseudomonas extremaustralis sp. nov., a Poly(3-hydroxybutyrate) producer isolated from an antarctic environment.	Lopez NI, Pettinari MJ, Stackebrandt E, Tribelli PM, Potter M, Steinbuchel A, Mendez BS	Curr Microbiol	10.1007/s00284-009-9469-9	2009	*

References

#7206

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17835

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#37664 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#82385

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID368972.1 )

#119157 Collection of Institut Pasteur ; Curators of the CIP; CIP 109839
* These data were automatically processed and therefore are not curated

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Pseudomonas extremaustralis strain DSM 17835

Pseudomonas extremaustralis strain DSM 17835

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