Frankia alni ACN14a, ULQ010201401 | Type strain | DSM 45986, CECT 9034 | BacDiveID:131722

Strain identifier

BacDive ID: 131722

Type strain:

Species: Frankia alni

Strain Designation: ACN14a, ULQ010201401

Culture col. no.: DSM 45986, CECT 9034

Strain history: <- M. Gtari, University of Tunis El Manar, Tunisia; ACN14a <- P. Normand, University Claude Bernard Lyon

NCBI tax ID(s): 1859 (species)

SI-ID 405251

DSM 45986

Section

Frankia alni ACN14a is an aerobe, heterotroph, spore-forming bacterium that was isolated from root nodule of Alnus viridis ssp. crispa.

Gram-positive
spore-forming
aerobe
heterotroph
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Frankiales*
	Family	*Frankiaceae*
	Genus	*Frankia*
	Species	**Frankia alni**
	Full Scientific Name (LPSN)	Frankia alni (Woronin 1866) Von Tubeuf 1895 (Approved Lists 1980)

	Synonym	"Schinzia* alni"*
	Synonym	"Frankia* subtilis"*

Information on morphological and physiological properties Morphology

[Ref.: #25097]	Gram stain	positive

[Ref.: #25097]	Colony color	white
[Ref.: #25097]	Cultivation medium used	BAP (basic propionate) medium with ammonium chloride

Multimedia content

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Information on culture and growth conditions Culture and growth conditions

Culture medium

BAP+ MEDIUM FOR FRANKIA (DSMZ Medium 1536)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

Culture medium

liquid BAP (basic propionate) medium

Culture medium growth

Culture medium

BAP (basic propionate) medium

Culture medium growth

Culture medium

MODIFIED BAP+ (PSA) MEDIUM FOR FRANKIA (DSMZ Medium 1660)

Culture medium growth

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #23518]			growth	28 °C
[Ref.: #25097]			optimum	28 °C

[Ref.: #23518]	Temperature range	mesophilic
[Ref.: #25097]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #25097]			growth	6.3-6.8

Information on physiology and metabolism Physiology and metabolism

Oxygen tolerance

aerobe

[Ref.: #25097]	Nutrition type	heterotroph
[Ref.: #25097]	Nutrition type	chemoorganotroph

[Ref.: #25097]	Type of spore	spore
[Ref.: #25097]	Ability of spore formation	yes

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25097]		(-)-quinic acid ChEBI	-	carbon source
[Ref.: #25097]		(S)-lactate ChEBI	-	carbon source
[Ref.: #25097]		2-hydroxybutyrate ChEBI	-	carbon source
[Ref.: #25097]		2-oxobutanoate ChEBI	-	carbon source
[Ref.: #25097]		2-oxoglutarate ChEBI	-	carbon source
[Ref.: #25097]		3-hydroxybutyrate ChEBI	-	carbon source
[Ref.: #25097]		3-O-methyl-D-glucose ChEBI	-	carbon source
[Ref.: #25097]		4-hydroxyphenylacetic acid ChEBI	-	carbon source
[Ref.: #25097]		acetoacetate ChEBI	-	carbon source
[Ref.: #25097]		bromosuccinate ChEBI	-	carbon source
[Ref.: #25097]		cellobiose ChEBI	-	carbon source
[Ref.: #25097]		D-arabitol ChEBI	-	carbon source
[Ref.: #25097]		D-aspartate ChEBI	-	carbon source
[Ref.: #25097]		D-fructose ChEBI	-	carbon source
[Ref.: #25097]		D-fructose 6-phosphate ChEBI	+	carbon source
[Ref.: #25097]		D-fucose ChEBI	-	carbon source
[Ref.: #25097]		D-galactose ChEBI	-	carbon source
[Ref.: #25097]		D-galacturonic acid ChEBI	-	carbon source
[Ref.: #25097]		D-glucarate ChEBI	-	carbon source
[Ref.: #25097]		D-gluconate ChEBI	-	carbon source
[Ref.: #25097]		D-glucose ChEBI	-	carbon source
[Ref.: #25097]		D-glucose 6-phosphate ChEBI	+	carbon source
[Ref.: #25097]		D-glucuronate ChEBI	-	carbon source
[Ref.: #25097]		D-malate ChEBI	-	carbon source
[Ref.: #25097]		D-mannitol ChEBI	-	carbon source
[Ref.: #25097]		D-mannose ChEBI	-	carbon source
[Ref.: #25097]		D-serine ChEBI	-	carbon source
[Ref.: #25097]		D-serine ChEBI	+	nitrogen source
[Ref.: #25097]		D-sorbitol ChEBI	-	carbon source
[Ref.: #25097]		dextrin ChEBI	-	carbon source
[Ref.: #25097]		fusidate ChEBI	+	growth
[Ref.: #25097]		galactarate ChEBI	-	carbon source
[Ref.: #25097]		gamma-aminobutyric acid ChEBI	-	carbon source
[Ref.: #25097]		gelatin ChEBI	-	carbon source
[Ref.: #25097]		gentiobiose ChEBI	-	carbon source
[Ref.: #25097]		glucuronamide ChEBI	-	carbon source
[Ref.: #25097]		glycerol ChEBI	-	carbon source
[Ref.: #25097]		glycine-proline ChEBI	-	carbon source
[Ref.: #25097]		glycyl L-aspartic acid ChEBI	-	carbon source
[Ref.: #25097]		guanidinium chloride ChEBI	+	nitrogen source
[Ref.: #25097]		inosine ChEBI	-	carbon source
[Ref.: #25097]		L-alanine ChEBI	-	carbon source
[Ref.: #25097]		L-arginine ChEBI	-	carbon source
[Ref.: #25097]		L-fucose ChEBI	-	carbon source
[Ref.: #25097]		L-galactonic acid gamma-lactone ChEBI	-	carbon source
[Ref.: #25097]		L-glutamate ChEBI	-	carbon source
[Ref.: #25097]		L-histidine ChEBI	-	carbon source
[Ref.: #25097]		L-malate ChEBI	+	carbon source
[Ref.: #25097]		L-pyroglutamic acid ChEBI	-	carbon source
[Ref.: #25097]		L-rhamnose ChEBI	-	carbon source
[Ref.: #25097]		L-serine ChEBI	-	carbon source
[Ref.: #25097]		lactose ChEBI	-	carbon source
[Ref.: #25097]		lithium chloride ChEBI	+	growth
[Ref.: #25097]		maltose ChEBI	-	carbon source
[Ref.: #25097]		melibiose ChEBI	-	carbon source
[Ref.: #25097]		methyl (R)-lactate ChEBI	-	carbon source
[Ref.: #25097]		methyl D-glucoside ChEBI	-	carbon source
[Ref.: #25097]		methyl pyruvate ChEBI	+	carbon source
[Ref.: #25097]		myo-inositol ChEBI	-	carbon source
[Ref.: #25097]		N-acetyl-beta-D-mannosamine ChEBI	-	carbon source
[Ref.: #25097]		N-acetylgalactosamine ChEBI	-	carbon source
[Ref.: #25097]		n-acetylneuraminate ChEBI	-	carbon source
[Ref.: #25097]		niaproof ChEBI	-	carbon source
[Ref.: #25097]		pectin ChEBI	-	carbon source
[Ref.: #25097]		potassium tellurite ChEBI	+	growth
[Ref.: #25097]		raffinose ChEBI	-	carbon source
[Ref.: #25097]		sodium bromate ChEBI	+	growth
[Ref.: #25097]		sodium formate ChEBI	-	carbon source
[Ref.: #25097]		sodium lactate ChEBI	+	growth
[Ref.: #25097]		stachyose ChEBI	-	carbon source
[Ref.: #25097]		sucrose ChEBI	-	carbon source
[Ref.: #25097]		trehalose ChEBI	-	carbon source
[Ref.: #25097]		turanose ChEBI	-	carbon source
		Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic resistance
[Ref.: #25097]		biphenyl ChEBI	yes
[Ref.: #25097]		lincomycin ChEBI	yes
[Ref.: #25097]		minocycline ChEBI	yes
[Ref.: #25097]		nalidixic acid ChEBI	yes
[Ref.: #25097]		polychlorobiphenyl ChEBI	yes
[Ref.: #25097]		vancomycin ChEBI	yes

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23518]	Sample type/isolated from	root nodule of Alnus viridis ssp. crispa
[Ref.: #23518]	Host species	Alnus viridis ssp. crispa
[Ref.: #23518]	Geographic location (country and/or sea, region)	Tadoussac
[Ref.: #23518]	Country	Canada
[Ref.: #23518]	Country ISO 3 Code	CAN
[Ref.: #23518]	Continent	North America

[Ref.: #25097]	Geographic location (country and/or sea, region)	Tadoussac
[Ref.: #25097]	Enrichment culture	BAP (basic propionate) medium
[Ref.: #25097]	Enrichment culture composition	supplemented with 0.5 mM NH4Cl as nitrogen source
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

Global distribution of 16S sequence KX674376 (>99% sequence identity) for Frankia from Microbeatlas

Aquatic Samples	53
Soil Samples	339
Animal Samples	79
Plant Samples	41
Total Samples	512

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

1

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #23518]	Frankia alni ACN14a strain ACN14A 16S ribosomal RNA gene, partial sequence	KX674376	1510	ENA	326424 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Frankia alni ACN14a	326424.15	complete	PATRIC	326424 tax ID	*
[Ref.: #66792]	Frankia alni ACN14a	637000115	complete	JGI IMG/M	326424 tax ID	*
[Ref.: #66792]	Frankia alni ACN14a	GCA_000058485	complete	GenBank	326424 tax ID	*

[Ref.: #25097]	GC-content	72.8 mol%
[Ref.: #23518]	GC-content	72.8 mol%	sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Frankia alni ACN14a PATRIC: 326424.15
[Ref.: #69480]	gram-positive	yes	88.724	yes
[Ref.: #69480]	anaerobic	no	98.335	yes
[Ref.: #69480]	halophile	no	97.469	no
[Ref.: #69480]	spore-forming	yes	75.891	no
[Ref.: #69480]	glucose-util	yes	71.949	yes
[Ref.: #69480]	motile	no	94.057	no
[Ref.: #69480]	flagellated	no	98.264	no
[Ref.: #69480]	aerobic	yes	88.008	yes
[Ref.: #69480]	thermophile	no	98.895	yes
[Ref.: #69480]	glucose-ferment	no	94.127	no

Availability in culture collections External links

Culture collection no.

DSM 45986, CECT 9034

SI-ID 405251

*

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Frankia torreyi sp. nov., the first actinobacterium of the genus Frankia Brunchorst 1886, 174(AL) isolated in axenic culture.	Nouioui I, Ghodhbane-Gtari F, Jando M, Tisa LS, Klenk HP, Gtari M	Antonie Van Leeuwenhoek	10.1007/s10482-018-1131-8	2018	*
Metabolism	Degradation of atrazine by Frankia alni ACN14a: gene regulation, dealkylation, and dechlorination.	Rehan M, Kluge M, Franzle S, Kellner H, Ullrich R, Hofrichter M	Appl Microbiol Biotechnol	10.1007/s00253-014-5665-z	2014	*
Phylogeny	The phylogeny of uptake hydrogenases in Frankia.	Leul M, Normand P, Sellstedt A	Int Microbiol	im2306118	2009	*
Metabolism	Biosynthetic gene cluster of cetoniacytone A, an unusual aminocyclitol from the endosymbiotic Bacterium Actinomyces sp. Lu 9419.	Wu X, Flatt PM, Xu H, Mahmud T	Chembiochem	10.1002/cbic.200800527	2009	*
Genetics	Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography.	Normand P, Lapierre P, Tisa LS, Gogarten JP, Alloisio N, Bagnarol E, Bassi CA, Berry AM, Bickhart DM, Choisne N, Couloux A, Cournoyer B, Cruveiller S, Daubin V, Demange N, Francino MP, Goltsman E, Huang Y, Kopp OR, Labarre L, Lapidus A, Lavire C, Marechal J, Martinez M, Mastronunzio JE, Mullin BC, Niemann J, Pujic P, Rawnsley T, Rouy Z, Schenowitz C, Sellstedt A, Tavares F, Tomkins JP, Vallenet D, Valverde C, Wall LG, Wang Y, Medigue C, Benson DR	Genome Res	10.1101/gr.5798407	2006	*
Metabolism	A Promiscuous Halogenase for the Derivatization of Flavonoids.	Kolling D, Stierhof M, Lasch C, Myronovskyi M, Luzhetskyy A	Molecules	10.3390/molecules26206220	2021	*
Metabolism	Molecular response to nitrogen starvation by Frankia alni ACN14a revealed by transcriptomics and functional analysis with a fosmid library in Escherichia coli.	Lurthy T, Alloisio N, Fournier P, Anchisi S, Ponsero A, Normand P, Pujic P, Boubakri H	Res Microbiol	10.1016/j.resmic.2017.12.002	2018	*
Phylogeny	Frankia inefficax sp. nov., an actinobacterial endophyte inducing ineffective, non nitrogen-fixing, root nodules on its actinorhizal host plants.	Nouioui I, Ghodhbane-Gtari F, Del Carmen Montero-Calasanz M, Rohde M, Tisa LS, Gtari M, Klenk HP	Antonie Van Leeuwenhoek	10.1007/s10482-016-0801-7	2016	*
Phylogeny	Proposal of a type strain for Frankia alni (Woronin 1866) Von Tubeuf 1895, emended description of Frankia alni, and recognition of Frankia casuarinae sp. nov. and Frankia elaeagni sp. nov.	Nouioui I, Ghodhbane-Gtari F, Montero-Calasanz MDC, Goker M, Meier-Kolthoff JP, Schumann P, Rohde M, Goodfellow M, Fernandez MP, Normand P, Tisa LS, Klenk HP, Gtari M	Int J Syst Evol Microbiol	10.1099/ijsem.0.001496	2016	*
Enzymology	Lectin genes in the Frankia alni genome.	Pujic P, Fournier P, Alloisio N, Hay AE, Marechal J, Anchisi S, Normand P	Arch Microbiol	10.1007/s00203-011-0770-1	2011	*
Phylogeny	The genus Micromonospora is widespread in legume root nodules: the example of Lupinus angustifolius.	Trujillo ME, Alonso-Vega P, Rodriguez R, Carro L, Cerda E, Alonso P, Martinez-Molina E	ISME J	10.1038/ismej.2010.55	2010	*
	A Stable Genetic Transformation System and Implications of the Type IV Restriction System in the Nitrogen-Fixing Plant Endosymbiont Frankia alni ACN14a.	Gifford I, Vance S, Nguyen G, Berry AM	Front Microbiol	10.3389/fmicb.2019.02230	2019	*
	Omics of the early molecular dialogue between Frankia alni and Alnus glutinosa and the cellulase synton.	Pujic P, Alloisio N, Fournier P, Roche D, Sghaier H, Miotello G, Armengaud J, Berry AM, Normand P	Environ Microbiol	10.1111/1462-2920.14606	2019	*
	A Nonspecific Lipid Transfer Protein with Potential Functions in Infection and Nodulation.	Gasser M, Alloisio N, Fournier P, Balmand S, Kharrat O, Tulumello J, Carro L, Heddi A, Da Silva P, Normand P, Pujic P, Boubakri H	Mol Plant Microbe Interact	10.1094/MPMI-06-22-0131-R	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23518

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45986

#25097

I. G.-G. Nouioui, F.,Montero-Calasanz, M. D.,Göker, M.,Meier-Kolthoff, J. P.,Schumann, P.,Rohde, M.,Goodfellow, M.,Fernandez, M. P.,Normand, P.,Tisa, L. S.,Klenk, H. P.,Gtari, M.: Proposal of a type strain for Frankia alni (Woronin 1866) Von Tubeuf 1895, emended description of Frankia alni, and recognition of Frankia casuarinae sp. nov. and Frankia elaeagni sp. nov. IJSEM 66: 5201 - 5210 2016 ( DOI 10.1099/ijsem.0.001496 , PubMed 27624710 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#90113

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405251.1 )

* These data were automatically processed and therefore are not curated

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