Frankia alni (DSM 45986, CECT 9034, ACN14a)

Name: Frankia alni (DSM 45986, CECT 9034, ACN14a)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 131722

Type strain

Strain Designation ACN14a, ULQ010201401

Culture col. no. DSM 45986 CECT 9034 ACN14a ULQ010201401

NCBI tax ID(s) 1859

Special Collections DSMZ Literature strains

History <- M. Gtari, University of Tunis El Manar, Tunisia; ACN14a <- P. Normand, University Claude Bernard Lyon

Links

Frankia alni ACN14a is an aerobe, heterotroph, spore-forming prokaryote that was isolated from root nodule of Alnus viridis ssp. crispa.

spore-forming Gram-positive aerobe heterotroph mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 405251

CECT 9034,DSM 45986

@ref 20215

Last LPSN update 2026-02-09 11:31:32

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Frankiales

Family Frankiaceae

Genus Frankia

Species Frankia alni

Full scientific name Frankia alni (Woronin 1866) Von Tubeuf 1895 (Approved Lists 1980)

Synonyms (2)

"Schinzia alni"
"Frankia subtilis"

Morphology

Cell morphology

@ref	Gram stain	Confidence
25097	positive
125439	positive	99.9
125439		92.7

Colony morphology

@ref	Colony color	Medium used
25097	white	BAP (basic propionate) medium with ammonium chloride

Multimedia

@ref:	66793
multimedia content:	EM_DSM_45986_1.jpg
multimedia.multimedia content:	EM_DSM_45986_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_45986_2.jpg
multimedia.multimedia content:	EM_DSM_45986_2.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_45986_3.jpg
multimedia.multimedia content:	EM_DSM_45986_3.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_45986_4.jpg
multimedia.multimedia content:	EM_DSM_45986_4.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_45986_5.jpg
multimedia.multimedia content:	EM_DSM_45986_5.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_45986_6.jpg
multimedia.multimedia content:	EM_DSM_45986_6.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_45986_7.jpg
multimedia.multimedia content:	EM_DSM_45986_7.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
23518	BAP+ MEDIUM FOR FRANKIA (DSMZ Medium 1536)	Medium recipe at MediaDive	Name: BAP+ MEDIUM FOR FRANKIA (DSMZ Medium 1536) Composition: KH2PO4 0.95 g/l K2HPO4 0.6 g/l Na-propionate 0.48 g/l NH4Cl 0.27 g/l MgSO4 x 7 H2O 0.025 g/l Citric acid 0.01 g/l Eisencitrat 0.01 g/l CaCl2 x 2 H2O 0.01 g/l H3BO3 0.00286 g/l MnCl2 x 4 H2O 0.00181 g/l Pyridoxine hydrochloride 0.0005 g/l Nicotinic acid 0.0005 g/l ZnSO4 x 7 H2O 0.00022 g/l Thiamine HCl 0.0001 g/l CuSO4 x 5 H2O 8e-05 g/l Na2MoO4 x 2 H2O 2.5e-05 g/l CoSO4 x 7 H2O 1e-06 g/l Distilled water
25097	liquid BAP (basic propionate) medium
25097	BAP (basic propionate) medium
23518	MODIFIED BAP+ (PSA) MEDIUM FOR FRANKIA (DSMZ Medium 1660)	Medium recipe at MediaDive	Name: MODIFIED BAP+ (PSA) MEDIUM FOR FRANKIA (DSMZ Medium 1660) Composition: KH2PO4 0.95 g/l K2HPO4 0.6 g/l Sodium acetate 0.48 g/l Na-propionate 0.48 g/l Pyruvic acid sodium salt 0.48 g/l Sodium succinate dibasic 0.48 g/l NH4Cl 0.27 g/l MgSO4 x 7 H2O 0.025 g/l Citric acid 0.01 g/l Eisencitrat 0.01 g/l CaCl2 x 2 H2O 0.01 g/l H3BO3 0.00286 g/l MnCl2 x 4 H2O 0.00181 g/l Nicotinic acid 0.0005 g/l Pyridoxine hydrochloride 0.0005 g/l ZnSO4 x 7 H2O 0.00022 g/l Thiamine HCl 0.0001 g/l CuSO4 x 5 H2O 8e-05 g/l Na2MoO4 x 2 H2O 2.5e-05 g/l CoSO4 x 7 H2O 1e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
23518	positive	growth	28	mesophilic
25097	positive	optimum	28	mesophilic

Culture pH

@ref	Ability	Type	PH
25097	positive	growth	6.3-6.8

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
25097	aerobe
125439	obligate aerobe	99.6

Nutrition type

@ref	Type
25097	heterotroph
25097	chemoorganotroph

Spore formation

@ref	Type of spore	Spore formation	Confidence
25097	spore
125439			93.1

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
25097	17521 ChEBI	(-)-quinic acid	-	carbon source
25097	16651 ChEBI	(S)-lactate	-	carbon source
25097	64552 ChEBI	2-hydroxybutyrate	-	carbon source
25097	16763 ChEBI	2-oxobutanoate	-	carbon source
25097	30916 ChEBI	2-oxoglutarate	-	carbon source
25097	37054 ChEBI	3-hydroxybutyrate	-	carbon source
25097	73918 ChEBI	3-O-methyl-D-glucose	-	carbon source
25097	18101 ChEBI	4-hydroxyphenylacetic acid	-	carbon source
25097	13705 ChEBI	acetoacetate	-	carbon source
25097	73706 ChEBI	bromosuccinate	-	carbon source
25097	17057 ChEBI	cellobiose	-	carbon source
25097	18333 ChEBI	D-arabitol	-	carbon source
25097	29990 ChEBI	D-aspartate	-	carbon source
25097	15824 ChEBI	D-fructose	-	carbon source
25097	78697 ChEBI	D-fructose 6-phosphate	+	carbon source
25097	28847 ChEBI	D-fucose	-	carbon source
25097	12936 ChEBI	D-galactose	-	carbon source
25097	18024 ChEBI	D-galacturonic acid	-	carbon source
25097	30612 ChEBI	D-glucarate	-	carbon source
25097	8391 ChEBI	D-gluconate	-	carbon source
25097	17634 ChEBI	D-glucose	-	carbon source
25097	14314 ChEBI	D-glucose 6-phosphate	+	carbon source
25097	15748 ChEBI	D-glucuronate	-	carbon source
25097	15588 ChEBI	D-malate	-	carbon source
25097	16899 ChEBI	D-mannitol	-	carbon source
25097	16024 ChEBI	D-mannose	-	carbon source
25097	16523 ChEBI	D-serine	-	carbon source
25097	16523 ChEBI	D-serine	+	nitrogen source
25097	17924 ChEBI	D-sorbitol	-	carbon source
25097	23652 ChEBI	dextrin	-	carbon source
25097	71321 ChEBI	fusidate	+	growth
25097	16537 ChEBI	galactarate	-	carbon source
25097	16865 ChEBI	gamma-aminobutyric acid	-	carbon source
25097	5291 ChEBI	gelatin	-	carbon source
25097	28066 ChEBI	gentiobiose	-	carbon source
25097	32323 ChEBI	glucuronamide	-	carbon source
25097	17754 ChEBI	glycerol	-	carbon source
25097	70744 ChEBI	glycine-proline	-	carbon source
25097	73804 ChEBI	glycyl L-aspartic acid	-	carbon source
25097	32735 ChEBI	guanidinium chloride	+	nitrogen source
25097	17596 ChEBI	inosine	-	carbon source
25097	16977 ChEBI	L-alanine	-	carbon source
25097	16467 ChEBI	L-arginine	-	carbon source
25097	18287 ChEBI	L-fucose	-	carbon source
25097	17464 ChEBI	L-galactonic acid gamma-lactone	-	carbon source
25097	29985 ChEBI	L-glutamate	-	carbon source
25097	15971 ChEBI	L-histidine	-	carbon source
25097	15589 ChEBI	L-malate	+	carbon source
25097	18183 ChEBI	L-pyroglutamic acid	-	carbon source
25097	62345 ChEBI	L-rhamnose	-	carbon source
25097	17115 ChEBI	L-serine	-	carbon source
25097	17716 ChEBI	lactose	-	carbon source
25097	48607 ChEBI	lithium chloride	+	growth
25097	17306 ChEBI	maltose	-	carbon source
25097	28053 ChEBI	melibiose	-	carbon source
25097	74611 ChEBI	methyl (R)-lactate	-	carbon source
25097	37657 ChEBI	methyl D-glucoside	-	carbon source
25097	51850 ChEBI	methyl pyruvate	+	carbon source
25097	17268 ChEBI	myo-inositol	-	carbon source
25097	63154 ChEBI	N-acetyl-beta-D-mannosamine	-	carbon source
25097	28037 ChEBI	N-acetylgalactosamine	-	carbon source
25097	35418 ChEBI	n-acetylneuraminate	-	carbon source
25097	75273 ChEBI	niaproof	-	carbon source
25097	17309 ChEBI	pectin	-	carbon source
25097	75248 ChEBI	potassium tellurite	+	growth
25097	16634 ChEBI	raffinose	-	carbon source
25097	75229 ChEBI	sodium bromate	+	growth
25097	62965 ChEBI	sodium formate	-	carbon source
25097	75228 ChEBI	sodium lactate	+	growth
25097	17164 ChEBI	stachyose	-	carbon source
25097	17992 ChEBI	sucrose	-	carbon source
25097	27082 ChEBI	trehalose	-	carbon source
25097	32528 ChEBI	turanose	-	carbon source

Antibiotic resistance

@ref	ChEBI	Metabolite
25097	17097	biphenyl
25097	6472	lincomycin
25097	50694	minocycline
25097	100147	nalidixic acid
25097	53156	polychlorobiphenyl
25097	28001	vancomycin

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Root nodule

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture composition
23518	root nodule of Alnus viridis ssp. crispa	Alnus viridis ssp. crispa	Tadoussac	Canada	CAN	North America
25097			Tadoussac				BAP (basic propionate) medium	supplemented with 0.5 mM NH4Cl as nitrogen source

Taxonmaps

69479

Global distribution of 16S sequence KX674376 (>99% sequence identity) for Frankia from Microbeatlas

Aquatic Samples	53
Soil Samples	339
Animal Samples	79
Plant Samples	41
Total Samples	512

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
23518	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM5848v1 assembly for Frankia alni ACN14a	complete	GCA_000058485	326424.15	637000115	326424	97.53

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
23518	Frankia alni ACN14a 16S ribosomal RNA gene, partial sequence	KX674376	1510		326424

GC content

@ref	GC-content (mol%)	Method
25097	72.8
23518	72.8	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Frankia alni ACN14a
PATRIC: 326424.15

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	93.10	no
125439	motility	BacteriaNetⓘ	no	92.70	no
125439	gram_stain	BacteriaNetⓘ	positive	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Frankia alni ACN14a
PATRIC: 326424.15

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	88.58	yes
125438	anaerobic	anaerobicⓘ	no	91.85	no
125438	spore-forming	spore-formingⓘ	yes	73.68	no
125438	aerobic	aerobicⓘ	yes	86.82	yes
125438	thermophilic	thermophileⓘ	no	91.90	yes
125438	flagellated	motile2+ⓘ	no	88.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Degradation of atrazine by Frankia alni ACN14a: gene regulation, dealkylation, and dechlorination.	Rehan M, Kluge M, Franzle S, Kellner H, Ullrich R, Hofrichter M	Appl Microbiol Biotechnol	10.1007/s00253-014-5665-z	2014
Phylogeny	The phylogeny of uptake hydrogenases in Frankia.	Leul M, Normand P, Sellstedt A	Int Microbiol	im2306118	2009
Metabolism	Biosynthetic gene cluster of cetoniacytone A, an unusual aminocyclitol from the endosymbiotic Bacterium Actinomyces sp. Lu 9419.	Wu X, Flatt PM, Xu H, Mahmud T	Chembiochem	10.1002/cbic.200800527	2009
Genetics	Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography.	Normand P, Lapierre P, Tisa LS, Gogarten JP, Alloisio N, Bagnarol E, Bassi CA, Berry AM, Bickhart DM, Choisne N, Couloux A, Cournoyer B, Cruveiller S, Daubin V, Demange N, Francino MP, Goltsman E, Huang Y, Kopp OR, Labarre L, Lapidus A, Lavire C, Marechal J, Martinez M, Mastronunzio JE, Mullin BC, Niemann J, Pujic P, Rawnsley T, Rouy Z, Schenowitz C, Sellstedt A, Tavares F, Tomkins JP, Vallenet D, Valverde C, Wall LG, Wang Y, Medigue C, Benson DR	Genome Res	10.1101/gr.5798407	2006
Metabolism	A Promiscuous Halogenase for the Derivatization of Flavonoids.	Kolling D, Stierhof M, Lasch C, Myronovskyi M, Luzhetskyy A	Molecules	10.3390/molecules26206220	2021
	A Stable Genetic Transformation System and Implications of the Type IV Restriction System in the Nitrogen-Fixing Plant Endosymbiont Frankia alni ACN14a.	Gifford I, Vance S, Nguyen G, Berry AM	Front Microbiol	10.3389/fmicb.2019.02230	2019
	Omics of the early molecular dialogue between Frankia alni and Alnus glutinosa and the cellulase synton.	Pujic P, Alloisio N, Fournier P, Roche D, Sghaier H, Miotello G, Armengaud J, Berry AM, Normand P	Environ Microbiol	10.1111/1462-2920.14606	2019
Metabolism	Molecular response to nitrogen starvation by Frankia alni ACN14a revealed by transcriptomics and functional analysis with a fosmid library in Escherichia coli.	Lurthy T, Alloisio N, Fournier P, Anchisi S, Ponsero A, Normand P, Pujic P, Boubakri H	Res Microbiol	10.1016/j.resmic.2017.12.002	2018
Enzymology	Lectin genes in the Frankia alni genome.	Pujic P, Fournier P, Alloisio N, Hay AE, Marechal J, Anchisi S, Normand P	Arch Microbiol	10.1007/s00203-011-0770-1	2011
Phylogeny	The genus Micromonospora is widespread in legume root nodules: the example of Lupinus angustifolius.	Trujillo ME, Alonso-Vega P, Rodriguez R, Carro L, Cerda E, Alonso P, Martinez-Molina E	ISME J	10.1038/ismej.2010.55	2010
	A Nonspecific Lipid Transfer Protein with Potential Functions in Infection and Nodulation.	Gasser M, Alloisio N, Fournier P, Balmand S, Kharrat O, Tulumello J, Carro L, Heddi A, Da Silva P, Normand P, Pujic P, Boubakri H	Mol Plant Microbe Interact	10.1094/MPMI-06-22-0131-R	2022
Phylogeny	Frankia torreyi sp. nov., the first actinobacterium of the genus Frankia Brunchorst 1886, 174(AL) isolated in axenic culture.	Nouioui I, Ghodhbane-Gtari F, Jando M, Tisa LS, Klenk HP, Gtari M	Antonie Van Leeuwenhoek	10.1007/s10482-018-1131-8	2018
Phylogeny	Frankia inefficax sp. nov., an actinobacterial endophyte inducing ineffective, non nitrogen-fixing, root nodules on its actinorhizal host plants.	Nouioui I, Ghodhbane-Gtari F, Del Carmen Montero-Calasanz M, Rohde M, Tisa LS, Gtari M, Klenk HP	Antonie Van Leeuwenhoek	10.1007/s10482-016-0801-7	2016
Phylogeny	Proposal of a type strain for Frankia alni (Woronin 1866) Von Tubeuf 1895, emended description of Frankia alni, and recognition of Frankia casuarinae sp. nov. and Frankia elaeagni sp. nov.	Nouioui I, Ghodhbane-Gtari F, Montero-Calasanz MDC, Goker M, Meier-Kolthoff JP, Schumann P, Rohde M, Goodfellow M, Fernandez MP, Normand P, Tisa LS, Klenk HP, Gtari M	Int J Syst Evol Microbiol	10.1099/ijsem.0.001496	2016

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23518	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45986
#25097	I. G.-G. Nouioui, F.,Montero-Calasanz, M. D.,Göker, M.,Meier-Kolthoff, J. P.,Schumann, P.,Rohde, M.,Goodfellow, M.,Fernandez, M. P.,Normand, P.,Tisa, L. S.,Klenk, H. P.,Gtari, M.: Proposal of a type strain for Frankia alni (Woronin 1866) Von Tubeuf 1895, emended description of Frankia alni, and recognition of Frankia casuarinae sp. nov. and Frankia elaeagni sp. nov. IJSEM 66: 5201 - 5210 2016 ( DOI 10.1099/ijsem.0.001496 , PubMed 27624710 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Frankia alni (DSM 45986, CECT 9034, ACN14a)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Metabolite utilization

Antibiotic resistance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000058485

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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