Name: Tsukamurella hongkongensis HKU52
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 131576

Type strain:

Species: Tsukamurella hongkongensis

Strain Designation: HKU52

Culture col. no.: DSM 100208, JCM 30715

Strain history: J. L. L. Teng; Dept. of Microbiol., Univ. of Hong Kong, China; HKU52.

NCBI tax ID(s): 1671023 (species)

SI-ID 397482

DSM 100208

Other strains from Tsukamurella hongkongensis

T. hongkongensis HKU53, DSM 100209, JCM 30716

Section

Tsukamurella hongkongensis HKU52 is an aerobe, mesophilic, Gram-positive human pathogen that forms irregular colonies and was isolated from human clinical sample, corneal scraping of patient with keratitis.

colony-forming
Gram-positive
aerobe
human pathogen
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Tsukamurellaceae*
	Genus	*Tsukamurella*
	Species	**Tsukamurella hongkongensis**
	Full Scientific Name (LPSN)	Tsukamurella hongkongensis Teng et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #44002]	Gram stain	positive

[Ref.: #44002]	Motility	no

[Ref.: #44002]	Colony size	2 mm
[Ref.: #44002]	Colony color	orange to red
[Ref.: #44002]	Colony shape	irregular
[Ref.: #44002]	Incubation period	2 days
[Ref.: #44002]	Cultivation medium used	Columbia agar with 5 % de?brinated sheep blood agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #44002]

Culture medium

Columbia agar with 5 % defibrinated sheep blood agar

[Ref.: #44002]

Culture medium growth

[Ref.: #22803]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #22803]

Culture medium growth

[Ref.: #22803]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22803]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #22803]		growth	37 °C
[Ref.: #44002]		growth	37 °C
[Ref.: #44002]		growth	42 °C
[Ref.: #44002]	no	growth	10 °C
[Ref.: #67770]		growth	37 °C

[Ref.: #22803]	Temperature range	mesophilic
[Ref.: #44002]	Temperature range	mesophilic
[Ref.: #44002]	Temperature range	thermophilic
[Ref.: #44002]	Temperature range	psychrophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44002]

Oxygen tolerance

aerobe

[Ref.: #44002]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #44002]		NaCl		growth	0-5	%

[Ref.: #44002]	Observation	dry and rough colonies
[Ref.: #44002]	Observation	Able to grow on brain heart infusion agar, nutrient agar, trypticase soy agar, chocolate agar and MacConkey agar
[Ref.: #44002]	Observation	major cell-wall sugars are galactose and arabinose,
[Ref.: #44002]	Observation	cell-wall peptidoglycan contains meso-diaminopimelic acid as the diagnostic diamino acid (chemotype IV) *

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #44002]	2-dehydro-D-gluconate ChEBI	-	assimilation
[Ref.: #44002]	5-dehydro-D-gluconate ChEBI	-	assimilation
[Ref.: #44002]	amygdalin ChEBI	-	assimilation
[Ref.: #44002]	arbutin ChEBI	-	assimilation
[Ref.: #44002]	cellobiose ChEBI	-	assimilation
[Ref.: #44002]	D-arabinose ChEBI	-	assimilation
[Ref.: #44002]	D-arabitol ChEBI	+	assimilation
[Ref.: #44002]	D-fructose ChEBI	+	assimilation
[Ref.: #44002]	D-fucose ChEBI	-	assimilation
[Ref.: #44002]	D-galactose ChEBI	+	assimilation
[Ref.: #44002]	D-glucose ChEBI	+	assimilation
[Ref.: #44002]	D-lyxose ChEBI	-	assimilation
[Ref.: #44002]	D-mannitol ChEBI	+	assimilation
[Ref.: #44002]	D-sorbitol ChEBI	+	assimilation
[Ref.: #44002]	D-tagatose ChEBI	-	assimilation
[Ref.: #44002]	D-trehalose ChEBI	+	assimilation
[Ref.: #44002]	D-xylose ChEBI	-	assimilation
[Ref.: #44002]	erythritol ChEBI	-	assimilation
[Ref.: #44002]	esculin ferric citrate	+	assimilation
[Ref.: #44002]	galactitol ChEBI	-	assimilation
[Ref.: #44002]	gentiobiose ChEBI	-	assimilation
[Ref.: #44002]	glycogen ChEBI	-	assimilation
[Ref.: #44002]	inulin ChEBI	-	assimilation
[Ref.: #44002]	L-arabinose ChEBI	-	assimilation
[Ref.: #44002]	L-arabitol ChEBI	-	assimilation
[Ref.: #44002]	L-fucose ChEBI	+	assimilation
[Ref.: #44002]	L-rhamnose ChEBI	-	assimilation
[Ref.: #44002]	L-sorbose ChEBI	-	assimilation
[Ref.: #44002]	L-xylose ChEBI	-	assimilation
[Ref.: #44002]	lactose ChEBI	-	assimilation
[Ref.: #44002]	maltose ChEBI	-	assimilation
[Ref.: #44002]	melezitose ChEBI	-	assimilation
[Ref.: #44002]	melibiose ChEBI	-	assimilation
[Ref.: #44002]	methyl alpha-D-mannoside ChEBI	-	assimilation
[Ref.: #44002]	methyl beta-D-xylopyranoside ChEBI	-	assimilation
[Ref.: #44002]	myo-inositol ChEBI	-	assimilation
[Ref.: #44002]	potassium gluconate ChEBI	+	assimilation
[Ref.: #44002]	raffinose ChEBI	-	assimilation
[Ref.: #44002]	ribitol ChEBI	-	assimilation
[Ref.: #44002]	salicin ChEBI	-	assimilation
[Ref.: #44002]	starch ChEBI	-	assimilation
[Ref.: #44002]	sucrose ChEBI	+	assimilation
[Ref.: #44002]	turanose ChEBI	+	assimilation
[Ref.: #44002]	tyrosine ChEBI	-	hydrolysis
[Ref.: #44002]	xanthine ChEBI	-	hydrolysis
[Ref.: #44002]	xylitol ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #44002]		catalase	+	1.11.1.6
[Ref.: #44002]		cytochrome oxidase	-	1.9.3.1

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #44002]

Fatty acid	Percentage
C18:1ω9c	31.4
C16:0	21.5
C16:1ω6c, C16:1ω7c and iso-C15:0 2-OH	15.2
10-Methyl C18:0	13.3
C14:0	4.5
C20:1ω9c	3.7
C16:1ω9c	2.6
C18:0	2
C17:1ω9c	1.4

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22803]	Sample type/isolated from	human clinical sample, corneal scraping of patient with keratitis
[Ref.: #22803]	Geographic location (country and/or sea, region)	Hong Kong, Queen Mary Hospital
[Ref.: #22803]	Country	China
[Ref.: #22803]	Country ISO 3 Code	CHN
[Ref.: #22803]	Continent	Asia

[Ref.: #44002]	Sample type/isolated from	corneal scraping of a patient with keratitis
[Ref.: #44002]	Geographic location (country and/or sea, region)	Hong-Kong
[Ref.: #44002]	Country	China
[Ref.: #44002]	Country ISO 3 Code	CHN
[Ref.: #44002]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Corneal scraping of a patient with keratitis
[Ref.: #67770]	Geographic location (country and/or sea, region)	Hong Kong
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Infection	#Medical environment	#Clinic
#Host	#Human	-
#Host Body-Site	#Organ	#Eye
#Infection	#Inflammation	-
#Infection	#Patient	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KP888572 (>99% sequence identity) for Tsukamurella from Microbeatlas

Aquatic Samples	2978
Soil Samples	3431
Animal Samples	5160
Plant Samples	790
Total Samples	12359

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #22803]	Pathogenicity (human)	yes, in single cases
[Ref.: #22803]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #22803]	Tsukamurella hongkongensis strain HKU52 16S ribosomal RNA gene, partial sequence	KP888572	1405	ENA	1671023 tax ID

[Ref.: #44002]	GC-content	69.2-73.4 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #22803]	GC-content	71.3 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	70.2-72.4 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #22803]

Culture collection no.

DSM 100208, JCM 30715

[Ref.: #89982]

SI-ID 397482

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Tsukamurella hongkongensis sp. nov. and Tsukamurella sinensis sp. nov., isolated from patients with keratitis, catheter-related bacteraemia and conjunctivitis.	Teng JLL, Tang Y, Wong SSY, Ngan AHY, Huang Y, Tsang CC, Choi GKY, Lau SKP, Woo PCY	Int J Syst Evol Microbiol	10.1099/ijsem.0.000733	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22803

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100208

#44002

Jade L. L. Teng, Ying Tang, Samson S. Y. Wong, Antonio H. Y. Ngan, Yi Huang, Chi-Ching Tsang, Garnet K. Y. Choi, Susanna K. P. Lau, Patrick C. Y. Woo: Tsukamurella hongkongensis sp. nov. and Tsukamurella sinensis sp. nov., isolated from patients with keratitis, catheter-related bacteraemia and conjunctivitis. IJSEM 66: 391 - 397 2016 ( DOI 10.1099/ijsem.0.000733 , PubMed 26530900 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#89982

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397482.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Tsukamurella hongkongensis in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF