Name: Demequina subtropica H12-128
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 131541

Type strain:

Species: Demequina subtropica

Strain Designation: H12-128, HI12-128

Culture col. no.: DSM 28145, NBRC 109396

Strain history: <- M. Hamada, NBRC, NITE (Natl. Inst. of Technol. and Evaluation), Kisarazu, Japan; HI12-128

NCBI tax ID(s): 1638989 (species)

SI-ID 403939

DSM 28145

Section

Demequina subtropica H12-128 is a mesophilic, Gram-positive bacterium that was isolated from rhizosphere soil of mangrove.

Gram-positive
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Demequinaceae*
	Genus	*Demequina*
	Species	**Demequina subtropica**
	Full Scientific Name (LPSN)	Demequina subtropica (Hamada et al. 2015) Nouioui et al. 2018

Synonym

Lysinimicrobium subtropicum

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

Information on culture and growth conditions Culture and growth conditions

[Ref.: #22768]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #22768]

Culture medium growth

[Ref.: #22768]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22768]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #22768]			growth	28 °C

[Ref.: #22768]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.996

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22768]	Sample type/isolated from	rhizosphere soil of mangrove
[Ref.: #22768]	Geographic location (country and/or sea, region)	Okinawa, Iriomote Island
[Ref.: #22768]	Country	Japan
[Ref.: #22768]	Country ISO 3 Code	JPN
[Ref.: #22768]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Mangrove
#Environmental	#Terrestrial	#Soil
#Host	#Plants	-
#Host Body-Site	#Plant	#Rhizosphere

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC042209 (>99% sequence identity) for Demequina from Microbeatlas

Aquatic Samples	11
Soil Samples	17
Animal Samples	15
Plant Samples
Total Samples	43

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #22768]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #22768]	Lysinimicrobium subtropicum gene for 16S ribosomal RNA, partial sequence	LC042209	1476	ENA	1638989 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Demequina subtropica NBRC 109396	GCA_000971235	scaffold	GenBank	1638989 tax ID	*
[Ref.: #66792]	Lysinimicrobium sp. NBRC 109396	1536638.3	wgs	PATRIC	1638989 tax ID	*
[Ref.: #66792]	Lysinimicrobium subtropicum NBRC 109396	2663763161	draft	JGI IMG/M	1638989 tax ID	*

[Ref.: #22768]

GC-content

71.6 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Demequina subtropica NBRC 109396 NCBI: GCA_000971235.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Demequina subtropica NBRC 109396 NCBI: GCA_000971235.1
[Ref.: #69480]	motile	yes	68.883	no
[Ref.: #69480]	flagellated	no	87.679	no
[Ref.: #69480]	gram-positive	yes	89.885	no
[Ref.: #69480]	anaerobic	no	97.931	no
[Ref.: #69480]	aerobic	no	70.216	no
[Ref.: #69480]	halophile	no	81.41	no
[Ref.: #69480]	spore-forming	no	90.124	no
[Ref.: #69480]	glucose-util	yes	83.741	no
[Ref.: #69480]	thermophile	no	97.565	yes
[Ref.: #69480]	glucose-ferment	no	62.434	no

Availability in culture collections External links

[Ref.: #22768]

Culture collection no.

DSM 28145, NBRC 109396

[Ref.: #89955]

SI-ID 403939

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Proposal of nine novel species of the genus Lysinimicrobium and emended description of the genus Lysinimicrobium.	Hamada M, Shibata C, Saitou S, Tamura T, Komaki H, Ichikawa N, Oguchi A, Hosoyama A, Fujita N, Yamamura H, Hayakawa M, Suzuki KI	Int J Syst Evol Microbiol	10.1099/ijsem.0.000587	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22768

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28145

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#89955

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403939.1 )

* These data were automatically processed and therefore are not curated

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Demequina subtropica NBRC 109396

Demequina subtropica NBRC 109396

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