Name: Demequina silvatica H12-123
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 131537

Type strain:

Species: Demequina silvatica

Strain Designation: H12-123, HI12-123

Culture col. no.: DSM 28147, NBRC 109395

Strain history: <- M. Hamada, NBRC, NITE (Natl. Inst. of Technol. and Evaluation), Kisarazu, Japan; HI12-123

NCBI tax ID(s): 1638988 (species)

SI-ID 401695

DSM 28147

Section

Demequina silvatica H12-123 is a mesophilic, Gram-positive bacterium that was isolated from soil of mangrove forest.

Gram-positive
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Demequinaceae*
	Genus	*Demequina*
	Species	**Demequina silvatica**
	Full Scientific Name (LPSN)	Demequina silvatica Nouioui et al. 2018

Synonym

Lysinimicrobium luteum

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %93.941

Information on culture and growth conditions Culture and growth conditions

[Ref.: #22764]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #22764]

Culture medium growth

[Ref.: #22764]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22764]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #22764]			growth	28 °C

[Ref.: #22764]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.994

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22764]	Sample type/isolated from	soil of mangrove forest
[Ref.: #22764]	Geographic location (country and/or sea, region)	Okinawa, Iriomote Island
[Ref.: #22764]	Country	Japan
[Ref.: #22764]	Country ISO 3 Code	JPN
[Ref.: #22764]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Mangrove
#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC042208 (>99% sequence identity) for Demequina from Microbeatlas

Aquatic Samples	48
Soil Samples	61
Animal Samples	137
Plant Samples	6
Total Samples	252

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #22764]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #22764]	Lysinimicrobium luteum gene for 16S ribosomal RNA, partial sequence	LC042208	1476	ENA	1638988 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Demequina silvatica NBRC 109395	GCA_000971215	scaffold	GenBank	1638988 tax ID	*
[Ref.: #66792]	Lysinimicrobium sp. NBRC 109395	1536637.3	wgs	PATRIC	1638988 tax ID	*
[Ref.: #66792]	Lysinimicrobium luteum NBRC 109395	2657244995	draft	JGI IMG/M	1638988 tax ID	*

[Ref.: #22764]

GC-content

72.0 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Demequina silvatica NBRC 109395 NCBI: GCA_000971215.1
[Ref.: #69481]	spore-forming	no	68	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Demequina silvatica NBRC 109395 NCBI: GCA_000971215.1
[Ref.: #69480]	motile	no	90.129	no
[Ref.: #69480]	flagellated	no	96.733	no
[Ref.: #69480]	gram-positive	yes	93.269	no
[Ref.: #69480]	anaerobic	no	98.185	no
[Ref.: #69480]	aerobic	no	56.529	no
[Ref.: #69480]	halophile	no	84.748	no
[Ref.: #69480]	spore-forming	no	89.489	no
[Ref.: #69480]	glucose-util	yes	84.147	no
[Ref.: #69480]	thermophile	no	98.516	yes
[Ref.: #69480]	glucose-ferment	no	66.852	no

Availability in culture collections External links

[Ref.: #22764]

Culture collection no.

DSM 28147, NBRC 109395

[Ref.: #89951]

SI-ID 401695

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Proposal of nine novel species of the genus Lysinimicrobium and emended description of the genus Lysinimicrobium.	Hamada M, Shibata C, Saitou S, Tamura T, Komaki H, Ichikawa N, Oguchi A, Hosoyama A, Fujita N, Yamamura H, Hayakawa M, Suzuki KI	Int J Syst Evol Microbiol	10.1099/ijsem.0.000587	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22764

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28147

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#89951

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401695.1 )

* These data were automatically processed and therefore are not curated

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Demequina silvatica NBRC 109395

Demequina silvatica NBRC 109395

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