Name: Lactococcus laudensis 4195
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 131360

Type strain:

Species: Lactococcus laudensis

Strain Designation: 4195, CRA-FLC-4195

Culture col. no.: DSM 28961, LMG 28353, JCM 31965, CIP 110947

Strain history: CIP <- 2015, M. Zago, CRA-FLC, Lodi, Italy: strain 4195

NCBI tax ID(s): 1494461 (species)

SI-ID 394243

LMG 28353

Special Collections

Section

Lactococcus laudensis 4195 is a microaerophile, Gram-positive bacterium that was isolated from cow raw milk.

Gram-positive
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Streptococcaceae*
	Genus	*Lactococcus*
	Species	**Lactococcus laudensis**
	Full Scientific Name (LPSN)	Lactococcus laudensis Meucci et al. 2015

Information on morphological and physiological properties Morphology

[Ref.: #120571]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %90.276

[Ref.: #120571]	Cell shape	coccus-shaped

[Ref.: #120571]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %90.5

Information on culture and growth conditions Culture and growth conditions

[Ref.: #22587]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #22587]

Culture medium growth

[Ref.: #22587]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22587]

Culture medium composition

expand / minimize

[Ref.: #120571]

Culture medium

CIP Medium 40

[Ref.: #120571]

Culture medium growth

[Ref.: #120571]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #120571]

Culture medium

CIP Medium 29

[Ref.: #120571]

Culture medium growth

[Ref.: #120571]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #22587]			growth	30 °C
[Ref.: #67770]			growth	30 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #22587]

Oxygen tolerance

microaerophile

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #22587]	14864	-	-	-	-	-	+	+	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+/-	+	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22587]	Sample type/isolated from	cow raw milk
[Ref.: #22587]	Geographic location (country and/or sea, region)	Lombardy, Valle Trompia
[Ref.: #22587]	Country	Italy
[Ref.: #22587]	Country ISO 3 Code	ITA
[Ref.: #22587]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Cow milk collected from a bovine herd in Valle Trompia
[Ref.: #67770]	Country	Italy
[Ref.: #67770]	Country ISO 3 Code	ITA
[Ref.: #67770]	Continent	Europe

[Ref.: #120571]	Sample type/isolated from	Food, Cow, raw milk
[Ref.: #120571]	Geographic location (country and/or sea, region)	Lombardy, Italy
[Ref.: #120571]	Country	Italy
[Ref.: #120571]	Country ISO 3 Code	ITA
[Ref.: #120571]	Continent	Europe
[Ref.: #120571]	Isolation date	1998-03-02
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Fluids	#Milk

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KJ394457 (>99% sequence identity) for Lactococcus from Microbeatlas

Aquatic Samples	1196
Soil Samples	815
Animal Samples	4033
Plant Samples	147
Total Samples	6191

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #22587]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120571]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #22587]	Lactococcus laudensis strain DSM 28961 16S ribosomal RNA gene, partial sequence	KJ394457	1478	GenBank ENA	1494461 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactococcus laudensis DSM 28961	GCA_013449735	contig	GenBank ENA	1494461 tax ID
[Ref.: #66792]	Lactococcus laudensis LMG 28353	GCA_019586025	contig	GenBank ENA	1494461 tax ID	*
[Ref.: #66792]	Lactococcus laudensis strain DSM 28961	1494461.5	wgs	PATRIC	1494461 tax ID	*
[Ref.: #66792]	Lactococcus laudensis strain LMG 28353	1494461.9	wgs	PATRIC	1494461 tax ID	*

[Ref.: #22587]	GC-content	41.1 mol%	fluorimetric
[Ref.: #67770]	GC-content	41.1 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lactococcus laudensis strain LMG 28353 PATRIC: 1494461.9
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactococcus laudensis strain DSM 28961 PATRIC: 1494461.5
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	90.276	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	88.255	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	82.985	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	97.656	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.81	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	90.5	no

Availability in culture collections External links

[Ref.: #22587]

Culture collection no.

DSM 28961, LMG 28353, JCM 31965, CIP 110947

[Ref.: #89799]

SI-ID 394243

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactococcus hircilactis sp. nov. and Lactococcus laudensis sp. nov., isolated from milk.	Meucci A, Zago M, Rossetti L, Fornasari ME, Bonvini B, Tidona F, Povolo M, Contarini G, Carminati D, Giraffa G	Int J Syst Evol Microbiol	10.1099/ijs.0.000225	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22587

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28961

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#89799

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID394243.1 )

#120571 Collection of Institut Pasteur ; Curators of the CIP; CIP 110947
* These data were automatically processed and therefore are not curated

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Lactococcus laudensis strain LMG 28353

Lactococcus laudensis strain DSM 28961

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