Name: Pseudomonas migulae CCUG 43165
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 13133

Type strain:

Species: Pseudomonas migulae

Culture col. no.: CCUG 43165, CCM 4843, DSM 17966, CIP 105470, CFML 95-321, JCM 21628, IAM 15173, NBRC 103157

Strain history: CIP <- 1998, D. Izard, Fac. Med. Henri Warenbourg, Lille, France: strain CFML 95-321

NCBI tax ID(s): 1215109 (strain), 78543 (species)

SI-ID 69809

CIP 105470, CCUG 43165, CCM 4843, NCIMB 13841, KACC 10813, DSM 17966, JCM 21628, CFBP 4843, NBRC 103157, VTT E-042571, IAM 15173

Special Collections

Other strains from Pseudomonas migulae

Section

When using BacDive for research please cite our paper

Pseudomonas migulae CCUG 43165 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from mineral water.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas migulae**
	Full Scientific Name (LPSN)	Pseudomonas migulae Verhille et al. 1999

Information on morphological and physiological properties Morphology

[Ref.: #120950]	Gram stain	negative
[Ref.: #125438]	Gram stain	negative Confidence in %99
[Ref.: #125439]	Gram stain	negative Confidence in %98.3

[Ref.: #120950]	Cell shape	rod-shaped

[Ref.: #120950]	Motility	yes
[Ref.: #125438]	Motility	yes Confidence in %90.151

[Ref.: #120950]	Pigment production	no
[Ref.: #120950]	Pigment name	Pyocyanin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7298]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #7298]

Culture medium growth

[Ref.: #7298]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7298]

Culture medium composition

expand / minimize

[Ref.: #37208]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37208]

Culture medium growth

[Ref.: #37208]

Culture medium composition

expand / minimize

[Ref.: #120950]

Culture medium

CIP Medium 72

[Ref.: #120950]

Culture medium growth

[Ref.: #120950]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #7298]		growth	30 °C
[Ref.: #37208]		growth	30 °C
[Ref.: #55704]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #120950]		growth	5-37 °C
[Ref.: #120950]	no	growth	41 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #55704]	Oxygen tolerance	aerobe
[Ref.: #120950]	Oxygen tolerance	obligate aerobe
[Ref.: #125438]	Oxygen tolerance	aerobe Confidence in %94.073
[Ref.: #125439]	Oxygen tolerance	obligate aerobe Confidence in %99.1

[Ref.: #125439]

Ability of spore formation

no Confidence in %98.6

	Salt	Tested relation		Salt conc.
[Ref.: #120950]	NaCl		growth	0-4	%
[Ref.: #120950]	NaCl	no	growth	6	%
[Ref.: #120950]	NaCl	no	growth	8	%
[Ref.: #120950]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #120950]	citrate ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #120950]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #120950]	nitrate ChEBI	-	reduction
[Ref.: #120950]	nitrate ChEBI	-	respiration
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #120950]	nitrite ChEBI	-	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120950]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #120950]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120950]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120950]	amylase	-
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #120950]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120950]	caseinase	-	3.4.21.50
[Ref.: #120950]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #120950]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #120950]	gelatinase	-
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #120950]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #120950]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120950]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120950]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120950]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120950]	tryptophan deaminase	-
[Ref.: #120950]	tween esterase	-
[Ref.: #120950]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #7298]	25575	-	-	-	+	-	-	-	-	+	+	+	+	+	-	+	+	-	+	+	+	+

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #120950]	1172	not determinedn.d.	+	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	+	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	+	+	-	-	+	+	+	+	+	+	+	+	+	+	-	-	+	+	+	+	+	+	-	-	+	-	-	-	+	+	+	+	+	+	+	-	+	+	+	+	+	+	-	+	-	+	+	+	+	+	+	+	+	+	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120950]	8726	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7298]	Sample type/isolated from	mineral water
[Ref.: #7298]	Geographic location (country and/or sea, region)	1
[Ref.: #7298]	Country	France
[Ref.: #7298]	Country ISO 3 Code	FRA
[Ref.: #7298]	Continent	Europe

[Ref.: #55704]	Sample type/isolated from	Mineral waters
[Ref.: #55704]	Sampling date	1995
[Ref.: #55704]	Country	France
[Ref.: #55704]	Country ISO 3 Code	FRA
[Ref.: #55704]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Mineral water

[Ref.: #120950]	Sample type/isolated from	Environment, Mineral waters
[Ref.: #120950]	Isolation date	1995
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF074383 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	207
Soil Samples	80
Animal Samples	151
Plant Samples	34
Total Samples	472

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7298]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120950]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7298]	Pseudomonas migulae 16S ribosomal RNA gene, complete sequence	AF074383	1516	GenBank ENA	78543 tax ID
[Ref.: #124043]	Pseudomonas migulae gene for 16S rRNA, partial sequence, strain: NBRC 103157.	AB681965	1462	GenBank ENA	78543 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Pseudomonas migulae NBRC 103157	GCA_002091715	contig	GenBank ENA	1215109 tax ID
[Ref.: #66792]	Pseudomonas migulae NBRC 103157 strain NBRC 103157	1215109.3	wgs	PATRIC	1215109 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudomonas migulae NBRC 103157 strain NBRC 103157 PATRIC: 1215109.3
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	99	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	98.414	no
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	80.723	no
[Ref.: #125438]	aerobic	aerobicⓘ	yes	94.073	yes
[Ref.: #125438]	thermophile	thermophilicⓘ	no	98	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	yes	90.151	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Pseudomonas migulae NBRC 103157 NCBI: GCA_002091715
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	99.1
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	98.3
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	85
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	98.6

Availability in culture collections External links

[Ref.: #7298]

Culture collection no.

CCUG 43165, CCM 4843, DSM 17966, CIP 105470, CFML 95-321, JCM 21628, IAM 15173, NBRC 103157

[Ref.: #82341]

SI-ID 69809

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas pohangensis sp. nov., isolated from seashore sand in Korea.	Weon HY, Kim BY, Yoo SH, Baek YK, Lee SY, Kwon SW, Go SJ, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/ijs.0.64274-0	2006	*
Phylogeny	Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea.	Kwon SW, Kim JS, Park IC, Yoon SH, Park DH, Lim CK, Go SJ	Int J Syst Evol Microbiol	10.1099/ijs.0.02326-0	2003	*
Phylogeny	Pseudomonas gessardii sp. nov. and Pseudomonas migulae sp. nov., two new species isolated from natural mineral waters.	Verhille S, Baida N, Dabboussi F, Hamze M, Izard D, Leclerc H	Int J Syst Bacteriol	10.1099/00207713-49-4-1559	1999	*

References

#7298

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17966

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#37208 ; Curators of the CIP;
#55704 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 43165

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#82341

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID69809.1 )

#120950 Collection of Institut Pasteur ; Curators of the CIP; CIP 105470

#124043

Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

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Pseudomonas migulae NBRC 103157 strain NBRC 103157

Pseudomonas migulae NBRC 103157

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