Pseudomonas abietaniphila (DSM 17554, ATCC 700689, CIP 106708)

Name: Pseudomonas abietaniphila (DSM 17554, ATCC 700689, CIP 106708)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13122

Type strain

Strain Designation BKME-9

Culture col. no. DSM 17554 ATCC 700689 CIP 106708 CCUG 50779 BKME-9

NCBI tax ID(s) 89065

Special Collections DSMZ CCUG CIP

History <- W. W. Mohn <- P. Bicho; BKME-9 CIP <- 2000, W.W. Mohn, British Columbia Univ., Vancouver, Canada: strain BKME-9 <- P. Bicho

Links

Pseudomonas abietaniphila BKME-9 is an aerobe, mesophilic, Gram-negative prokaryote that was isolated from bleached kraft mill effluent.

Gram-negative motile rod-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 44082

ATCC 700689,LMG 20220,CIP 106708,CCUG 50779,DSM 17554

@ref 20215

Last LPSN update 2026-02-09 11:21:03

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Pseudomonas

Species Pseudomonas abietaniphila

Full scientific name Pseudomonas abietaniphila Mohn et al. 1999

BacDive ID	Other strains from **Pseudomonas abietaniphila** (1)	Type strain
12832	P. abietaniphila Berkeley 53-17, 109, DSM 6506, ATCC 17483, ...

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
115975	negative	rod-shaped
125439	negative		99.1
125438			90.682
125438	negative		99

Pigmentation

@ref	Production	Name
115975		Pyocyanin

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6970	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
40385	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
115975	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6970	positive	growth	28	mesophilic
40385	positive	growth	30	mesophilic
58992	positive	growth	30	mesophilic
115975	positive	growth	5-30
115975	negative	growth	37
115975	negative	growth	41

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
58992	aerobe
115975	obligate aerobe
125438	aerobe	93.478
125439	obligate aerobe	96.1

Spore formation

@ref	Spore formation	Confidence
125439		99

Halophily

@ref	Salt	Growth	Tested relation	Concentration
115975	NaCl	positive	growth	0-6 %
115975	NaCl		growth	8 %
115975	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
115975	16947 ChEBI	citrate	+	carbon source
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
115975	4853 ChEBI	esculin	-	hydrolysis
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
115975	17632 ChEBI	nitrate	-	reduction
115975	17632 ChEBI	nitrate	-	respiration
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
115975	16301 ChEBI	nitrite	-	reduction
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
115975	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
115975	35581 ChEBI	indole
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
115975	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
115975	amylase	+
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
115975	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
115975	caseinase	-	3.4.21.50
115975	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	-	1.9.3.1	from API 20NE
115975	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
115975	gelatinase	+/-
68369	gelatinase	-		from API 20NE
115975	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
115975	lipase	-
68382	lipase (C 14)	+		from API zym
115975	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
115975	ornithine decarboxylase	-	4.1.1.17
115975	oxidase	-
115975	protease	-
68382	trypsin	-	3.4.21.4	from API zym
115975	tryptophan deaminase	-
115975	tween esterase	+
115975	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
115975	-	+	+	+	+	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
6970	-	-	-	-	-	-	-	-	+	+	+	+	-	-	+	+	-	+	+	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
115975	not determinedn.d.	+	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	+	+	-	+	+	+	-	-	+	+	+	-	-	-	+	-	-	-	-	-	+	+	+	+	+	+	+	+	+	+	+	-	-	+	-	-	-	+	-	+	+	+	-	-	-	-	-	-	+	+	+	-	+	+	+	-	+	+	+	+	+	+	-	+	+	+	+	+	+	+	+	+	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Industrial wastewater

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
6970	bleached kraft mill effluent		Kamloops, B.C.	Canada	CAN	North America
58992	Bleached kraft mill effluent	1995	British Columbia	Canada	CAN	North America
115975	Bleached kraft mill effluent		British Columbia	Canada	CAN	North America	1995

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6970	1	Risk group (German classification) According to TRBA 466
115975	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	IMG-taxon 2667527211 annotated assembly for Pseudomonas abietaniphila ATCC 700689	contig	GCA_900100795	89065.12	2667527211	89065	67.44

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6970	Pseudomonas abietaniphila partial 16S rRNA gene, type strain ATCC 700689T	AJ011504	1448		89065

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Pseudomonas abietaniphila ATCC 700689
NCBI: GCA_900100795

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.00	no
125439	motility	BacteriaNetⓘ	yes	88.50	no
125439	gram_stain	BacteriaNetⓘ	negative	99.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudomonas abietaniphila strain ATCC 700689
PATRIC: 89065.12

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.00	no
125438	anaerobic	anaerobicⓘ	no	98.32	yes
125438	aerobic	aerobicⓘ	yes	93.48	yes
125438	spore-forming	spore-formingⓘ	no	79.19	no
125438	thermophilic	thermophileⓘ	no	100.00	yes
125438	flagellated	motile2+ⓘ	yes	90.68	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Grey mould control by oxalate degradation using non-antifungal Pseudomonas abietaniphila strain ODB36.	Lee Y, Choi O, Kang B, Bae J, Kim S, Kim J.	Sci Rep	10.1038/s41598-020-58609-z	2020
Phylogeny	Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria.	Mellmann A, Cloud J, Maier T, Keckevoet U, Ramminger I, Iwen P, Dunn J, Hall G, Wilson D, Lasala P, Kostrzewa M, Harmsen D.	J Clin Microbiol	10.1128/jcm.00157-08	2008
Metabolism	A large gene cluster in Burkholderia xenovorans encoding abietane diterpenoid catabolism.	Smith DJ, Park J, Tiedje JM, Mohn WW	J Bacteriol	10.1128/JB.00179-07	2007
Phylogeny	Molecular detection and diversity of novel diterpenoid dioxygenase DitA1 genes from proteobacterial strains and soil samples.	Witzig R, Aly HA, Strompl C, Wray V, Junca H, Pieper DH	Environ Microbiol	10.1111/j.1462-2920.2007.01242.x	2007
Metabolism	A cytochrome P450 involved in the metabolism of abietane diterpenoids by Pseudomonas abietaniphila BKME-9.	Smith DJ, Martin VJ, Mohn WW	J Bacteriol	10.1128/JB.186.11.3631-3639.2004	2004
Metabolism	Characterization of tdt genes for the degradation of tricyclic diterpenes by Pseudomonas diterpeniphila A19-6a.	Morgan CA, Wyndham RC	Can J Microbiol	10.1139/w01-127	2002
Metabolism	Bioaugmentation with resin-acid-degrading bacteria enhances resin acid removal in sequencing batch reactors treating pulp mill effluents.	Yu Z, Mohn WW	Water Res	10.1016/s0043-1354(00)00335-3	2001
Metabolism	Genetic investigation of the catabolic pathway for degradation of abietane diterpenoids by Pseudomonas abietaniphila BKME-9.	Martin VJ, Mohn WW	J Bacteriol	10.1128/JB.182.13.3784-3793.2000	2000
Metabolism	Recent advances in understanding resin acid biodegradation: microbial diversity and metabolism.	Martin VJ, Yu Z, Mohn WW	Arch Microbiol	10.1007/s002030050752	1999
Metabolism	Occurrence of Two Resin Acid-Degrading Bacteria and a Gene Encoding Resin Acid Biodegradation in Pulp and Paper Mill Effluent Biotreatment Systems Assayed by PCR.	Yu Z, Martin VJ, Mohn WW	Microb Ecol	10.1007/s002489900163	1999
Metabolism	A novel aromatic-ring-hydroxylating dioxygenase from the diterpenoid-degrading bacterium Pseudomonas abietaniphila BKME-9.	Martin VJ, Mohn WW	J Bacteriol	10.1128/JB.181.9.2675-2682.1999	1999
Enzymology	An alternative inverse PCR (IPCR) method to amplify DNA sequences flanking Tn5 transposon insertions.	Martin VJ, Mohn WW	J Microbiol Methods	10.1016/s0167-7012(98)00115-8	1999
Phylogeny	Discovery of Phloeophagus Beetles as a Source of Pseudomonas Strains That Produce Potentially New Bioactive Substances and Description of Pseudomonas bohemica sp. nov.	Saati-Santamaria Z, Lopez-Mondejar R, Jimenez-Gomez A, Diez-Mendez A, Vetrovsky T, Igual JM, Velazquez E, Kolarik M, Rivas R, Garcia-Fraile P	Front Microbiol	10.3389/fmicb.2018.00913	2018

References

#6970	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17554
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#40385	; Curators of the CIP;
#58992	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 50779
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#115975	Collection of Institut Pasteur ; Curators of the CIP; CIP 106708
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudomonas abietaniphila (DSM 17554, ATCC 700689, CIP 106708)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900100795

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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