Pandoraea pnomenusa (DSM 101008, B356)

Name: Pandoraea pnomenusa (DSM 101008, B356)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 131102

Type strain

Strain Designation B356

Culture col. no. DSM 101008 B356

NCBI tax ID(s) 93220

Special Collections DSMZ

Links

Pandoraea pnomenusa B356 is a mesophilic prokaryote that was isolated from activated sludge from water treatment system.

mesophilic 16S sequence

Name and taxonomic classification

SI-ID 406796

DSM 101008

@ref 20215

Last LPSN update 2025-12-16 10:01:10

Domain Pseudomonadati

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Pandoraea

Species Pandoraea pnomenusa

Full scientific name Pandoraea pnomenusa Coenye et al. 2000

BacDive ID	Other strains from **Pandoraea pnomenusa** (12)	Type strain
2060	P. pnomenusa R140, DSM 16536, CCM 4978, CCUG 38742, CIP ... (type strain)
150326	P. pnomenusa CCUG 39672
150336	P. pnomenusa CCUG 39683, LMG 18820
150341	P. pnomenusa CCUG 39689, LMG 18817
150345	P. pnomenusa CCUG 39694
150934	P. pnomenusa CCUG 42779
151921	P. pnomenusa CCUG 45204
151969	P. pnomenusa CCUG 45402
152999	P. pnomenusa CCUG 48224
154511	P. pnomenusa CCUG 53975
157134	P. pnomenusa CCUG 69688
158043	P. pnomenusa DSM 100868, RB 38

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
22394	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)		Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
22394	MINERAL MEDIUM WITH VITAMINS (DSMZ Medium 462)		Medium recipe at MediaDive	Name: MINERAL MEDIUM (BRUNNER) WITH VITAMINS (DSMZ Medium 462) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Casein peptone 15.0 g/l Peptone 5.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Meat extract 3.0 g/l Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l Pyridoxamine 0.000125 g/l ZnSO4 x 7 H2O 0.0001 g/l Nicotinic acid 5e-05 g/l Vitamin B12 5e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l MnCl2 x 4 H2O 3e-05 g/l p-Aminobenzoate 2.5e-05 g/l Thiamine-HCl x 2 H2O 2.5e-05 g/l NiCl2 x 6 H2O 2e-05 g/l Calcium pantothenate 1.25e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Biotin 5e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
22394	positive	growth	28	mesophilic

Physiology and metabolism

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68369	cytochrome oxidase	-	1.9.3.1	from API 20NE
68369	gelatinase	-		from API 20NE
68369	urease	-	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
22394	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	+	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Activated sludge
#Engineered	#Waste	#Wastewater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
22394	activated sludge from water treatment system	Montreal	Canada	CAN	North America

Taxonmaps

69479

Global distribution of 16S sequence EF596910 (>99% sequence identity) for Pandoraea from Microbeatlas

Aquatic Samples	4192
Soil Samples	6943
Animal Samples	3605
Plant Samples	5843
Total Samples	20583

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
22394	2	Risk group (German classification)

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
22394	Pandoraea pnomenusa strain B-356 16S ribosomal RNA gene, partial sequence	EF596910	1434		93220

Genome-based predictions

Literature

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#22394	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101008
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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