Name: Caldibacillus thermolactis B3188
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1311

Type strain:

Species: Caldibacillus thermolactis

Strain Designation: B3188, MB2765, R-6488

Culture col. no.: DSM 23332, LMG 25569

Strain history: <- A. Coorevits, Lab. Microbiology, Ghent Univ., Belgium; R-6488 <- P. Scheldeman, Agricultural Res. Centre, Ministry of the Flemish Community

NCBI tax ID(s): 251051 (species)

SI-ID 20563

DSM 23332, LMG 25569

Section

Caldibacillus thermolactis B3188 is a thermophilic bacterium that was isolated from raw milk.

thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Caldibacillus*
	Species	**Caldibacillus thermolactis**
	Full Scientific Name (LPSN)	Caldibacillus thermolactis (Coorevits et al. 2011) Gupta et al. 2020

	Synonym	Bacillus thermolactis
	Synonym	Pallidibacillus thermolactis

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16800]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #16800]

Culture medium growth

[Ref.: #16800]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16800]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #16800]			growth	55 °C

[Ref.: #16800]

Temperature range

thermophilic

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16800]	Sample type/isolated from	raw milk
[Ref.: #16800]	Country	Belgium
[Ref.: #16800]	Country ISO 3 Code	BEL
[Ref.: #16800]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host Body Product	#Fluids	#Milk
#Host	#Mammals	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY397764 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	4000
Soil Samples	15537
Animal Samples	9415
Plant Samples	4460
Total Samples	33412

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16800]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16800]	Bacillus thermolactis strain R-6488 16S ribosomal RNA gene, partial sequence	AY397764	1466	ENA	251051 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Caldibacillus thermolactis DSM 23332	GCA_025660415	scaffold	GenBank	251051 tax ID	*

[Ref.: #16800]

GC-content

35 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Caldibacillus thermolactis DSM 23332 NCBI: GCA_025660415.1
[Ref.: #69480]	motile	yes	90.102	no
[Ref.: #69480]	flagellated	yes	81.297	no
[Ref.: #69480]	gram-positive	yes	92.674	no
[Ref.: #69480]	anaerobic	no	98.06	no
[Ref.: #69480]	aerobic	yes	91.203	no
[Ref.: #69480]	halophile	yes	82.166	no
[Ref.: #69480]	spore-forming	yes	94.505	no
[Ref.: #69480]	glucose-ferment	no	88.859	no
[Ref.: #69480]	thermophile	yes	68.321	no
[Ref.: #69480]	glucose-util	yes	87.99	no

Availability in culture collections External links

[Ref.: #16800]

Culture collection no.

DSM 23332, LMG 25569

[Ref.: #70962]

SI-ID 20563

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus pasinlerensis sp. nov., a thermophilic bacterium isolated from a hot spring in Turkey.	Baltaci MO, Ay H, Akbulut S, Adiguzel G, Albayrak S, Omeroglu MA, Ozkan H, Taskin M, Adiguzel A	Int J Syst Evol Microbiol	10.1099/ijsem.0.004246	2020	*
Phylogeny	Bacillus hisashii sp. nov., isolated from the caeca of gnotobiotic mice fed with thermophile-fermented compost.	Nishida A, Miyamoto H, Horiuchi S, Watanabe R, Morita H, Fukuda S, Ohno H, Ichinose S, Miyamoto H, Kodama H	Int J Syst Evol Microbiol	10.1099/ijsem.0.000516	2015	*
Phylogeny	Thermotolerant Bacillus kokeshiiformis sp. nov. isolated from marine animal resources compost.	Poudel P, Miyamoto H, Miyamoto H, Okugawa Y, Tashiro Y, Sakai K	Int J Syst Evol Microbiol	10.1099/ijs.0.059329-0	2014	*
Phylogeny	Bacillus thermolactis sp. nov., isolated from dairy farms, and emended description of Bacillus thermoamylovorans.	Coorevits A, Logan NA, Dinsdale AE, Halket G, Scheldeman P, Heyndrickx M, Schumann P, Van Landschoot A, De Vos P	Int J Syst Evol Microbiol	10.1099/ijs.0.024240-0	2010	*

References

#16800

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23332

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#70962

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID20563.1 )

* These data were automatically processed and therefore are not curated

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Caldibacillus thermolactis DSM 23332

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