Name: Pseudomonas antarctica DSM 15318
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13093

Type strain:

Species: Pseudomonas antarctica

Culture col. no.: DSM 15318, CCUG 49625, CIP 108466, CMS 35, MTCC 4992

Strain history: CIP <- 2004, S. Shivaji, CCMB, Hyderabad, India: strain CMS 35

NCBI tax ID(s): 219572 (species)

SI-ID 131539

DSM 15318, LMG 22709, CCUG 49625, CIP 108466, CCM 7749

Other strains from Pseudomonas antarctica

P. antarctica DSM 15320, CMS 45

Section

Pseudomonas antarctica DSM 15318 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from cyanobacterial mat samples.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas antarctica**
	Full Scientific Name (LPSN)	Pseudomonas antarctica Reddy et al. 2004

Information on morphological and physiological properties Morphology

[Ref.: #30011]	Gram stain	negative
[Ref.: #116401]	Gram stain	negative

[Ref.: #30011]	Cell shape	rod-shaped
[Ref.: #116401]	Cell shape	rod-shaped

[Ref.: #30011]	Motility	yes
[Ref.: #116401]	Motility	yes

[Ref.: #58636]

Incubation period

2 days

[Ref.: #30011]	Pigment production	no
[Ref.: #116401]	Pigment production	no
[Ref.: #116401]	Pigment name	Flexirubin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5841]

Culture medium

REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)

[Ref.: #5841]

Culture medium growth

[Ref.: #5841]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5841]

Culture medium composition

expand / minimize

[Ref.: #40004]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #40004]

Culture medium growth

[Ref.: #40004]

Culture medium composition

expand / minimize

[Ref.: #116401]

Culture medium

CIP Medium 72

[Ref.: #116401]

Culture medium growth

[Ref.: #116401]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5841]		growth	28 °C
[Ref.: #30011]		growth	04-30 °C
[Ref.: #30011]		optimum	22 °C
[Ref.: #40004]		growth	25 °C
[Ref.: #58636]		growth	20-30 °C
[Ref.: #116401]		growth	5-30 °C
[Ref.: #116401]	no	growth	37 °C
[Ref.: #116401]	no	growth	41 °C

[Ref.: #5841]	Temperature range	mesophilic
[Ref.: #30011]	Temperature range	psychrophilic
[Ref.: #40004]	Temperature range	mesophilic
[Ref.: #116401]	Temperature range	mesophilic
[Ref.: #116401]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #30011]			optimum	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30011]	Oxygen tolerance	aerobe
[Ref.: #58636]	Oxygen tolerance	aerobe
[Ref.: #116401]	Oxygen tolerance	obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #30011]	NaCl		growth	0-3	%
[Ref.: #116401]	NaCl		growth	0-6	%
[Ref.: #116401]	NaCl	no	growth	8	%
[Ref.: #116401]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30011]	acetate ChEBI	+	carbon source
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #30011]	alanine ChEBI	+	carbon source
[Ref.: #30011]	arginine ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #30011]	citrate ChEBI	+	carbon source
[Ref.: #116401]	citrate ChEBI	-	carbon source
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #30011]	D-tryptophan ChEBI	+	carbon source
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #116401]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #30011]	fructose ChEBI	+	carbon source
[Ref.: #30011]	galactose ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #30011]	glucose ChEBI	+	carbon source
[Ref.: #30011]	glutamate ChEBI	+	carbon source
[Ref.: #30011]	glycerol ChEBI	+	carbon source
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #30011]	lactate ChEBI	+	carbon source
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #30011]	mannitol ChEBI	+	carbon source
[Ref.: #30011]	mannose ChEBI	+	carbon source
[Ref.: #30011]	myo-inositol ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #30011]	nitrate ChEBI	+	reduction
[Ref.: #116401]	nitrate ChEBI	+	reduction
[Ref.: #116401]	nitrate ChEBI	-	respiration
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #116401]	nitrite ChEBI	-	reduction
[Ref.: #30011]	pyruvate ChEBI	+	carbon source
[Ref.: #30011]	ribitol ChEBI	+	carbon source
[Ref.: #30011]	ribose ChEBI	+	carbon source
[Ref.: #30011]	serine ChEBI	+	carbon source
[Ref.: #30011]	sorbitol ChEBI	+	carbon source
[Ref.: #30011]	trehalose ChEBI	+	carbon source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #116401]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #116401]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #30011]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #116401]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #30011]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #116401]	amylase	-
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #116401]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116401]	caseinase	-	3.4.21.50
[Ref.: #30011]	catalase	+	1.11.1.6
[Ref.: #116401]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #30011]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #116401]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #116401]	gelatinase	-
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #116401]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #116401]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116401]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116401]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #116401]	oxidase	+
[Ref.: #116401]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116401]	tryptophan deaminase	+
[Ref.: #116401]	tween esterase	-
[Ref.: #30011]	urease	+	3.5.1.5
[Ref.: #116401]	urease	+	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #5841]	23213	-	-	-	+	-	-	-	-	+	+	+	+	+	-	+	+	-	+	+	-	+
[Ref.: #5841]	22876	-	-	-	+	-	-	-	-	+	+	+	+	+	-	+	+	-	+	+	-	+

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #116401]	2759	not determinedn.d.	+	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	+	+	+	-	-	+	+	+	-	-	+	+	-	+	+	-	-	+	+	-	-	+	+	+	+	+	-	+	+	+	+	+	-	+	+	-	+	+	+	-	-	-	-	-	-	+	+	+	+	+	+	+	-	+	+	+	+	+	+	-	+	-	+	+	+	+	+	+	+	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116401]	11205	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5841]	Sample type/isolated from	cyanobacterial mat samples
[Ref.: #5841]	Geographic location (country and/or sea, region)	McMurdo
[Ref.: #5841]	Continent	Australia and Oceania

[Ref.: #58636]	Sample type/isolated from	Cyanobacterial mat samples
[Ref.: #58636]	Country	Antarctica
[Ref.: #58636]	Country ISO 3 Code	ATA
[Ref.: #58636]	Continent	Australia and Oceania

[Ref.: #116401]	Sample type/isolated from	Cyanobacterial materials
[Ref.: #116401]	Country	Antarctica
[Ref.: #116401]	Country ISO 3 Code	ATA
[Ref.: #116401]	Continent	Antarctica
* marker position based on {}

Isolation sources categories

#Environmental	#Microbial community	#Microbial mat
#Host	#Microbial	#Bacteria

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ537601 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	976
Soil Samples	555
Animal Samples	2173
Plant Samples	1759
Total Samples	5463

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5841]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116401]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #30011]	Pseudomonas antarctica 16S rRNA gene, strain CMS 35T	AJ537601	1511	GenBank	219572 tax ID

[Ref.: #5841]	GC-content	56 mol%
[Ref.: #30011]	GC-content	60.7 mol%

Availability in culture collections External links

[Ref.: #5841]

Culture collection no.

DSM 15318, CCUG 49625, CIP 108466, CMS 35, MTCC 4992

[Ref.: #82303]

SI-ID 131539

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas petroselini sp. nov., a pathogen causing bacterial rot of parsley in Japan.	Sawada H, Fujikawa T, Osada S, Satou M	Int J Syst Evol Microbiol	10.1099/ijsem.0.005424	2022	*
Phylogeny	Pseudomonas cyclaminis sp. nov., a pathogen causing bacterial bud blight of cyclamen in Japan.	Sawada H, Fujikawa T, Osada S, Satou M	Int J Syst Evol Microbiol	10.1099/ijsem.0.004723	2021	*
Phylogeny	Pseudomonas kitaguniensis sp. nov., a pathogen causing bacterial rot of Welsh onion in Japan.	Sawada H, Fujikawa T, Nishiwaki Y, Horita H	Int J Syst Evol Microbiol	10.1099/ijsem.0.004123	2020	*
Phylogeny	Psychrophilic pseudomonads from Antarctica: Pseudomonas antarctica sp. nov., Pseudomonas meridiana sp. nov. and Pseudomonas proteolytica sp. nov.	Reddy GSN, Matsumoto GI, Schumann P, Stackebrandt E, Shivaji S	Int J Syst Evol Microbiol	10.1099/ijs.0.02827-0	2004	*

References

#5841

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15318

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30011

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26375 (see below)

#40004 ; Curators of the CIP;
#58636 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 49625

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#82303

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID131539.1 )

#116401 Collection of Institut Pasteur ; Curators of the CIP; CIP 108466

#26375

IJSEM 713 2004 ( DOI 10.1099/ijs.0.02827-0 , PubMed 15143013 )

* These data were automatically processed and therefore are not curated

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