Pseudomonas extremorientalis (DSM 15824, KMM 3447, LMG 19695)

Name: Pseudomonas extremorientalis (DSM 15824, KMM 3447, LMG 19695)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13091

Type strain

Strain Designation FEB RAS

Culture col. no. DSM 15824 KMM 3447 LMG 19695 CCUG 51517 FEB RAS 1 more

NCBI tax ID(s) 169669

Special Collections DSMZ CCUG CIP

History <- LMG <- E. Ivanova; FEB RAS 2003, E. P. Ivanova, Hawthorn, Australia CIP <- 2003, E. P. Ivanova, Hawthorn, Australia

Links

Pseudomonas extremorientalis FEB RAS is an aerobe, mesophilic, Gram-negative prokaryote that was isolated from drinking water reservoir.

Gram-negative motile rod-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 13465

LMG 19695,DSM 15824,CCUG 51517,CFBP 6729,CIP 107815,CCM 7750

@ref 20215

Last LPSN update 2026-02-09 11:21:02

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Pseudomonas

Species Pseudomonas extremorientalis

Full scientific name Pseudomonas extremorientalis Ivanova et al. 2002

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
119158	negative	rod-shaped
125439	negative		93.9
125438			94.024
125438	negative		99

Pigmentation

@ref	Production	Name
119158		Pyocyanin

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6036	CASO AGAR (MERCK 105458) (DSMZ Medium 220)	Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
36642	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119158	CIP Medium 72	Medium recipe at CIP
6036	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
6036	TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)	Medium recipe at MediaDive	Name: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) Composition: Casein peptone 17.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
6036	PSEUDOMONAS AGAR F (DSMZ Medium 907)	Medium recipe at MediaDive	Name: PSEUDOMONAS AGAR F (DSMZ Medium 907) Composition: Agar 15.0 g/l Glycerol 10.0 g/l Proteose peptone 10.0 g/l Tryptone 10.0 g/l MgSO4 1.5 g/l K2HPO4 1.5 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6036	positive	growth	28	mesophilic
36642	positive	growth	25	mesophilic
59227	positive	growth	28	mesophilic
119158	positive	growth	5-30
119158	negative	growth	37
119158	negative	growth	41

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
59227	aerobe
119158	obligate aerobe
125438	aerobe	94.479

Spore formation

@ref	Spore formation	Confidence
125439		98.5

Compound production

6036

Compoundcyclic depsipeptide

Halophily

@ref	Salt	Growth	Tested relation	Concentration
119158	NaCl	positive	growth	2-8 %
119158	NaCl		growth	0 %
119158	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	+	assimilation	from API 20NE
68369	29016 ChEBI	arginine	+	hydrolysis	from API 20NE
119158	16947 ChEBI	citrate	-	carbon source
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
119158	4853 ChEBI	esculin	-	hydrolysis
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	+	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
119158	17632 ChEBI	nitrate	+	reduction
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
119158	16301 ChEBI	nitrite	-	reduction
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
119158	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE
119158	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
119158	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
119158	amylase	-
68369	arginine dihydrolase	+	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
119158	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119158	caseinase	+	3.4.21.50
119158	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
119158	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
119158	gelatinase	-
68369	gelatinase	+		from API 20NE
119158	lecithinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
119158	lipase	-
68382	lipase (C 14)	-		from API zym
119158	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119158	ornithine decarboxylase	-	4.1.1.17
119158	oxidase	+
119158	protease	+
68382	trypsin	+	3.4.21.4	from API zym
119158	tryptophan deaminase	-
119158	tween esterase	+
119158	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119158	-	+	+	-	-	+	+	-	+	+	-	+	-	-	-	+	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
6036	-	-	-	+	-	-	+	-	+	+	+	+	+	-	+	+	+	+	+	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater
#Engineered	#Built environment	#Water reservoir (Aquarium/pool)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6036	drinking water reservoir	Vladivostock City	Russia	RUS	Asia
59227	Water,drinking water reservoir	Vladivostok	Russia	RUS	Asia
119158	Environment, Driking water reservoir		Russian Federation	RUS	Europe

Taxonmaps

69479

Global distribution of 16S sequence AF405328 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	976
Soil Samples	555
Animal Samples	2173
Plant Samples	1759
Total Samples	5463

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6036	1	Risk group (German classification) According to TRBA 466
119158	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	IMG-taxon 2639762715 annotated assembly for Pseudomonas extremorientalis BS2774	chromosome	GCA_900104365	169669.6	2639762715	169669	89.59
66792	ASM187046v1 assembly for Pseudomonas extremorientalis LMG 19695	contig	GCA_001870465	169669.5	8117383975	169669	67.17
66792	ASM880156v1 assembly for Pseudomonas extremorientalis CCUG 51517	contig	GCA_008801565	169669.10		169669	64.96

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
6036	Pseudomonas extremorientalis 16S ribosomal RNA gene, partial sequence	AF405328	1516	169669
124043	Pseudomonas extremorientalis culture DSM:15824 16S ribosomal RNA gene, partial sequence.	KX186943	1431	169669
124043	Pseudomonas extremorientalis strain KMM 3447 16S ribosomal RNA gene, partial sequence.	OP164746	1441	169669

GC content

6036

GC-content (mol%)61

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Pseudomonas extremorientalis LMG 19695
NCBI: GCA_001870465

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	98.50	no
125439	motility	BacteriaNetⓘ	yes	80.90	no
125439	gram_stain	BacteriaNetⓘ	negative	93.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	87.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudomonas extremorientalis LMG 19695
NCBI: GCA_900104365.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.00	no
125438	anaerobic	anaerobicⓘ	no	98.91	yes
125438	aerobic	aerobicⓘ	yes	94.48	yes
125438	spore-forming	spore-formingⓘ	no	87.31	no
125438	thermophilic	thermophileⓘ	no	99.00	yes
125438	flagellated	motile2+ⓘ	yes	94.02	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Zwitterionic poly(sulfobetaine methacrylate)-based hydrogel coating for drinking water distribution systems to inhibit adhesion of waterborne bacteria.	Sojka O, van der Mei HC, van Rijn P, Gagliano MC.	Front Bioeng Biotechnol	10.3389/fbioe.2023.1066126	2023
	Genome assembly of Pseudomonas sp. strain SED1^T, a psychrotolerant bacterium isolated from Deception Glacier (Washington, USA).	Shain DH, Klein EA.	Microbiol Resour Announc	10.1128/mra.00125-24	2024
Genetics	Agronomic efficiency and genome mining analysis of the wheat-biostimulant rhizospheric bacterium Pseudomonas pergaminensis sp. nov. strain 1008^T.	Diaz M, Bach T, Gonzalez Anta G, Agaras B, Wibberg D, Noguera F, Canciani W, Valverde C.	Front Plant Sci	10.3389/fpls.2022.894985	2022
Phylogeny	Pseudomonas allii sp. nov., a pathogen causing soft rot of onion in Japan.	Sawada H, Fujikawa T, Tsuji M, Satou M	Int J Syst Evol Microbiol	10.1099/ijsem.0.004582	2020
Phylogeny	Pseudomonas extremorientalis sp. nov., isolated from a drinking water reservoir.	Ivanova EP, Gorshkova NM, Sawabe T, Hayashi K, Kalinovskaya NI, Lysenko AM, Zhukova NV, Nicolau DV, Kuznetsova TA, Mikhailov VV, Christen R	Int J Syst Evol Microbiol	10.1099/00207713-52-6-2113	2002

References

#6036	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15824
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#36642	; Curators of the CIP;
#59227	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 51517
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119158	Collection of Institut Pasteur ; Curators of the CIP; CIP 107815
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudomonas extremorientalis (DSM 15824, KMM 3447, LMG 19695)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900104365

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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