Name: Pseudomonas extremorientalis FEB RAS
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13091

Type strain:

Species: Pseudomonas extremorientalis

Strain Designation: FEB RAS

Culture col. no.: DSM 15824, KMM 3447, LMG 19695, CCUG 51517, CIP 107815

Strain history: CIP <- 2003, E. P. Ivanova, Hawthorn, Australia

NCBI tax ID(s): 169669 (species)

SI-ID 13465

LMG 19695, DSM 15824, CCUG 51517, CFBP 6729, CIP 107815, CCM 7750

Section

Pseudomonas extremorientalis FEB RAS is an aerobe, mesophilic, Gram-negative bacterium that was isolated from drinking water reservoir.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas extremorientalis**
	Full Scientific Name (LPSN)	Pseudomonas extremorientalis Ivanova et al. 2002

Information on morphological and physiological properties Morphology

[Ref.: #119158]	Gram stain	negative

[Ref.: #119158]	Cell shape	rod-shaped

[Ref.: #119158]	Motility	yes

[Ref.: #119158]	Pigment production	no
[Ref.: #119158]	Pigment name	Pyocyanin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6036]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #6036]

Culture medium growth

[Ref.: #6036]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6036]

Culture medium composition

expand / minimize

[Ref.: #36642]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #36642]

Culture medium growth

[Ref.: #36642]

Culture medium composition

expand / minimize

[Ref.: #119158]

Culture medium

CIP Medium 72

[Ref.: #119158]

Culture medium growth

[Ref.: #119158]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #6036]		growth	28 °C
[Ref.: #36642]		growth	25 °C
[Ref.: #59227]		growth	28 °C
[Ref.: #119158]		growth	5-30 °C
[Ref.: #119158]	no	growth	37 °C
[Ref.: #119158]	no	growth	41 °C

[Ref.: #6036]	Temperature range	mesophilic
[Ref.: #36642]	Temperature range	mesophilic
[Ref.: #59227]	Temperature range	mesophilic
[Ref.: #119158]	Temperature range	mesophilic
[Ref.: #119158]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #59227]	Oxygen tolerance	aerobe
[Ref.: #119158]	Oxygen tolerance	obligate aerobe

[Ref.: #6036]

Name of produced compound

cyclic depsipeptide

	Salt	Tested relation		Salt conc.
[Ref.: #119158]	NaCl		growth	2-8	%
[Ref.: #119158]	NaCl	no	growth	0	%
[Ref.: #119158]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #119158]	citrate ChEBI	-	carbon source
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #119158]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #119158]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #119158]	nitrite ChEBI	-	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #119158]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #119158]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #119158]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #119158]	amylase	-
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #119158]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119158]	caseinase	+	3.4.21.50
[Ref.: #119158]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #119158]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #119158]	gelatinase	-
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #119158]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #119158]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119158]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119158]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119158]	oxidase	+
[Ref.: #119158]	protease	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #119158]	tryptophan deaminase	-
[Ref.: #119158]	tween esterase	+
[Ref.: #119158]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #6036]	22877	-	-	-	+	-	-	+	-	+	+	+	+	+	-	+	+	+	+	+	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119158]	10669	-	+	+	-	-	+	+	-	+	+	-	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6036]	Sample type/isolated from	drinking water reservoir
[Ref.: #6036]	Geographic location (country and/or sea, region)	Vladivostock City
[Ref.: #6036]	Country	Russia
[Ref.: #6036]	Country ISO 3 Code	RUS
[Ref.: #6036]	Continent	Asia

[Ref.: #59227]	Sample type/isolated from	Water,drinking water reservoir
[Ref.: #59227]	Geographic location (country and/or sea, region)	Vladivostok
[Ref.: #59227]	Country	Russia
[Ref.: #59227]	Country ISO 3 Code	RUS
[Ref.: #59227]	Continent	Asia

[Ref.: #119158]	Sample type/isolated from	Environment, Driking water reservoir
[Ref.: #119158]	Country	Russian Federation
[Ref.: #119158]	Country ISO 3 Code	RUS
[Ref.: #119158]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Freshwater
#Engineered	#Built environment	#Water reservoir (Aquarium/pool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF405328 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	976
Soil Samples	555
Animal Samples	2173
Plant Samples	1759
Total Samples	5463

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6036]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119158]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6036]	Pseudomonas extremorientalis 16S ribosomal RNA gene, partial sequence	AF405328	1516	ENA	169669 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas extremorientalis LMG 19695	GCA_001870465	contig	GenBank	169669 tax ID	*
[Ref.: #66792]	Pseudomonas extremorientalis CCUG 51517	GCA_008801565	contig	GenBank	169669 tax ID	*
[Ref.: #66792]	Pseudomonas extremorientalis strain CCUG 51517	169669.10	wgs	PATRIC	169669 tax ID	*
[Ref.: #66792]	Pseudomonas extremorientalis strain LMG 19695	169669.5	wgs	PATRIC	169669 tax ID	*
[Ref.: #66792]	Pseudomonas extremorientalis LMG 19695	2639762715	draft	JGI IMG/M	169669 tax ID	*
[Ref.: #66792]	Pseudomonas extremorientalis LMG 19695	GCA_900104365	chromosome	GenBank	169669 tax ID	*

[Ref.: #6036]

GC-content

61 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas extremorientalis LMG 19695 NCBI: GCA_900104365.1
[Ref.: #69480]	motile	yes	91.192	no
[Ref.: #69480]	flagellated	yes	83.682	no
[Ref.: #69480]	gram-positive	no	98.374	no
[Ref.: #69480]	anaerobic	no	98.674	yes
[Ref.: #69480]	aerobic	yes	95.924	yes
[Ref.: #69480]	halophile	no	89.84	no
[Ref.: #69480]	spore-forming	no	96.387	no
[Ref.: #69480]	glucose-ferment	no	90.588	yes
[Ref.: #69480]	thermophile	no	99.88	yes
[Ref.: #69480]	glucose-util	yes	94.752	no

Availability in culture collections External links

[Ref.: #6036]

Culture collection no.

DSM 15824, KMM 3447, LMG 19695, CCUG 51517, CIP 107815

[Ref.: #82301]

SI-ID 13465

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas allii sp. nov., a pathogen causing soft rot of onion in Japan.	Sawada H, Fujikawa T, Tsuji M, Satou M	Int J Syst Evol Microbiol	10.1099/ijsem.0.004582	2020	*
Phylogeny	Pseudomonas extremorientalis sp. nov., isolated from a drinking water reservoir.	Ivanova EP, Gorshkova NM, Sawabe T, Hayashi K, Kalinovskaya NI, Lysenko AM, Zhukova NV, Nicolau DV, Kuznetsova TA, Mikhailov VV, Christen R	Int J Syst Evol Microbiol	10.1099/00207713-52-6-2113	2002	*

References

#6036

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15824

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#36642 ; Curators of the CIP;
#59227 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 51517

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#82301

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID13465.1 )

#119158 Collection of Institut Pasteur ; Curators of the CIP; CIP 107815
* These data were automatically processed and therefore are not curated

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Pseudomonas extremorientalis LMG 19695

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