Name: Alkanibacter difficilis MN154.3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130802

Type strain:

Species: Alkanibacter difficilis

Strain Designation: MN154.3

Culture col. no.: DSM 14804, LMG 22842

Strain history: <- M. M. Friedrich and A. Lipski, University Osnabrueck

NCBI tax ID(s): 159478 (species)

SI-ID 134196

LMG 22842, DSM 14804

Section

Alkanibacter difficilis MN154.3 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from industrial hexane degrading biofilter.

Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Nevskiales*
	Family	*Nevskiaceae*
	Genus	*Alkanibacter*
	Species	**Alkanibacter difficilis**
	Full Scientific Name (LPSN)	Alkanibacter difficilis Friedrich and Lipski 2008

Information on morphological and physiological properties Morphology

[Ref.: #32452]	Gram stain	negative

[Ref.: #32452]	Cell length	0.9 µm

[Ref.: #32452]	Cell width	0.6 µm

[Ref.: #32452]	Cell shape	rod-shaped

[Ref.: #21469]

Incubation period

>14 days

[Ref.: #32452]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21469]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #21469]

Culture medium growth

[Ref.: #21469]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21469]

Culture medium composition

expand / minimize

[Ref.: #21469]

Culture medium

MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)

[Ref.: #21469]

Culture medium growth

[Ref.: #21469]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21469]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #21469]	growth	30 °C
[Ref.: #32452]	growth	23-37 °C
[Ref.: #32452]	optimum	30 °C

[Ref.: #21469]	Temperature range	mesophilic
[Ref.: #32452]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #32452]			optimum	7
[Ref.: #32452]			growth	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32452]

Oxygen tolerance

aerobe

[Ref.: #32452]

Ability of spore formation

[Ref.: #32452]

Observation

aggregates in chains

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32452]		3-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #32452]		succinate ChEBI	+	carbon source

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #32452]		catalase	+	1.11.1.6
[Ref.: #32452]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21469]	Sample type/isolated from	industrial hexane degrading biofilter
[Ref.: #21469]	Geographic location (country and/or sea, region)	Hamm
[Ref.: #21469]	Country	Germany
[Ref.: #21469]	Country ISO 3 Code	DEU
[Ref.: #21469]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Bioremediation	#Biofilter
#Engineered	#Industrial	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ313020 (>99% sequence identity) for Alkanibacter difficilis subclade from Microbeatlas

Aquatic Samples	140
Soil Samples	354
Animal Samples	116
Plant Samples	31
Total Samples	641

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21469]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21469]	gamma proteobacteria MN 154.3 16S rRNA gene, isolate MN 154.3	AJ313020	1438	ENA	159478 tax ID

[Ref.: #21469]

GC-content

62.8 mol%

Availability in culture collections External links

[Ref.: #21469]

Culture collection no.

DSM 14804, LMG 22842

[Ref.: #89290]

SI-ID 134196

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Panacagrimonas perspica gen. nov., sp. nov., a novel member of Gammaproteobacteria isolated from soil of a ginseng field.	Im WT, Liu QM, Yang JE, Kim MS, Kim SY, Lee ST, Yi TH	J Microbiol	10.1007/s12275-010-0067-0	2010	*
Phylogeny	Alkanibacter difficilis gen. nov., sp. nov. and Singularimonas variicoloris gen. nov., sp. nov., hexane-degrading bacteria isolated from a hexane-treated biofilter.	Friedrich MM, Lipski A	Int J Syst Evol Microbiol	10.1099/ijs.0.65517-0	2008	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21469

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14804

#32452

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28674 (see below)

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#89290

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID134196.1 )

#28674

IJSEM 2324 2008 ( DOI 10.1099/ijs.0.65517-0 , PubMed 18842850 )

* These data were automatically processed and therefore are not curated

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