Leisingera aquaemixtae (DSM 29957, CECT 8399, KCTC 32538)

Name: Leisingera aquaemixtae (DSM 29957, CECT 8399, KCTC 32538)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 130799

Type strain

Strain Designation SSK6-1

Culture col. no. DSM 29957 CECT 8399 KCTC 32538 SSK6-1

NCBI tax ID(s) 1396826

Special Collections DSMZ KCTC

History DSMZ <- CECT <- J. H. Yoon, Sungkyunkwan Univ., Suwon, Republic of Korea

Links

Leisingera aquaemixtae SSK6-1 is a bacterium that was isolated from water collected from the zone where the ocean and a freshwater spring meet.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 393361

KCTC 32538,CECT 8399,DSM 29957

@ref 20215

Last LPSN update 2026-05-29 08:37:28

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodobacterales

Family Roseobacteraceae

Genus Leisingera

Species Leisingera aquaemixtae

Full scientific name Leisingera aquaemixtae (Park et al. 2014) Breider et al. 2014

Synonyms (1)

Phaeobacter aquaemixtae

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	92.972
125438	negative	99.5

Colony morphology

21858

Incubation period2-3 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21858	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)		Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
21858	positive	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	aerobe	90.792

Spore formation

@ref	Spore formation	Confidence
125439		98.863
125438		90.103

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	-	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
21858	catalase	+	1.11.1.6
21858	cytochrome-c oxidase	+	1.9.3.1
68369	gelatinase	-		from API 20NE
68369	urease	-	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
21858	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Spring

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
21858	water collected from the zone where the ocean and a freshwater spring meet	Jeju Island	Republic of Korea	KOR	Asia

Taxonmaps

69479

Global distribution of 16S sequence KF554505 (>99% sequence identity) for Leisingera aquaemixtae from Microbeatlas

Aquatic Samples	207
Soil Samples	5
Animal Samples	10
Plant Samples	0
Total Samples	222

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
21858	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	L.aquaemixtaeCECT8399_CISA_Prokka assembly for Leisingera aquaemixtae CECT 8399	contig	GCA_001458395	1396826.3	2713896966	1396826	69.48

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21858	Leisingera aquaemixtae strain SSK6-1 16S ribosomal RNA gene, partial sequence	KF554505	1386		1396826

GC content

@ref	GC-content (mol%)	Method
21858	64.6	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: L.aquaemixtaeCECT8399_CISA_Prokka assembly for Leisingera aquaemixtae CECT 8399
NCBI: GCA_001458395

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	90.79	no
125439	motility	BacteriaNetⓘ	yes	68.80	no
125439	gram_stain	BacteriaNetⓘ	negative	92.97	no
125439	spore_formation	BacteriaNetⓘ	no	98.86	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Leisingera aquaemixtae strain CECT 8399
PATRIC: 1396826.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.50	no
125438	anaerobic	anaerobicⓘ	no	94.42	no
125438	spore-forming	spore-formingⓘ	no	90.10	no
125438	aerobic	aerobicⓘ	yes	81.27	no
125438	thermophilic	thermophileⓘ	no	96.50	yes
125438	flagellated	motile2+ⓘ	yes	69.80	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Draft genome of Leisingera aquaemixtae CECT 8399(T), a member of the Roseobacter clade isolated from a junction of fresh and ocean water in Jeju Island, South Korea.	Rodrigo-Torres L, Pujalte MJ, Arahal DR	Genom Data	10.1016/j.gdata.2016.01.010	2016
	Leisingera thetidis sp. nov., isolated from coastal water.	Kim MJ, Guan Y, Choe H, Li Z, Chung N, Kang YH, Lee MK.	Int J Syst Evol Microbiol	10.1099/ijsem.0.005945	2023
Phylogeny	Cribrihabitans neustonicus sp. nov., isolated from coastal surface seawater, and emended description of the genus Cribrihabitans Chen et al. 2014.	Hameed A, Shahina M, Lin SY, Lai WA, Liu YC, Hsu YH, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.066142-0	2014
Phylogeny	Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring.	Park S, Park DS, Bae KS, Yoon JH	Int J Syst Evol Microbiol	10.1099/ijs.0.057646-0	2014

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21858	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29957
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Leisingera aquaemixtae (DSM 29957, CECT 8399, KCTC 32538)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001458395

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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