Name: Leisingera aquaemixtae SSK6-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130799

Type strain:

Species: Leisingera aquaemixtae

Strain Designation: SSK6-1

Culture col. no.: DSM 29957, CECT 8399, KCTC 32538

Strain history: <- CECT <- J. H. Yoon, Sungkyunkwan Univ., Suwon, Republic of Korea

NCBI tax ID(s): 1396826 (species)

SI-ID 393361

KCTC 32538, CECT 8399, DSM 29957

Section

Leisingera aquaemixtae SSK6-1 is a mesophilic bacterium that was isolated from water collected from the zone where the ocean and a freshwater spring meet.

mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodobacterales*
	Family	*Roseobacteraceae*
	Genus	*Leisingera*
	Species	**Leisingera aquaemixtae**
	Full Scientific Name (LPSN)	Leisingera aquaemixtae (Park et al. 2014) Breider et al. 2014

Synonym

Phaeobacter aquaemixtae

Information on morphological and physiological properties Morphology

[Ref.: #21858]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21858]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #21858]

Culture medium growth

[Ref.: #21858]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21858]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #21858]			growth	30 °C

[Ref.: #21858]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #21858]	catalase	+	1.11.1.6
[Ref.: #21858]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #21858]	24085	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21858]	Sample type/isolated from	water collected from the zone where the ocean and a freshwater spring meet
[Ref.: #21858]	Geographic location (country and/or sea, region)	Jeju Island
[Ref.: #21858]	Country	Republic of Korea
[Ref.: #21858]	Country ISO 3 Code	KOR
[Ref.: #21858]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Spring

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KF554505 (>99% sequence identity) for Leisingera aquaemixtae from Microbeatlas

Aquatic Samples	207
Soil Samples	5
Animal Samples	10
Plant Samples
Total Samples	222

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21858]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21858]	Leisingera aquaemixtae strain SSK6-1 16S ribosomal RNA gene, partial sequence	KF554505	1386	ENA	1396826 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Leisingera aquaemixtae CECT 8399	GCA_001458395	contig	GenBank	1396826 tax ID	*
[Ref.: #66792]	Leisingera aquaemixtae strain CECT 8399	1396826.3	wgs	PATRIC	1396826 tax ID	*
[Ref.: #66792]	Leisingera aquaemixtae CECT 8399	2713896966	draft	JGI IMG/M	1396826 tax ID	*

[Ref.: #21858]

GC-content

64.6 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Leisingera aquaemixtae strain CECT 8399 PATRIC: 1396826.3
[Ref.: #69480]	motile	yes	81.536	no
[Ref.: #69480]	gram-positive	no	98.48	no
[Ref.: #69480]	anaerobic	no	98.933	no
[Ref.: #69480]	halophile	yes	86.957	no
[Ref.: #69480]	spore-forming	no	95.944	no
[Ref.: #69480]	glucose-util	yes	64.381	yes
[Ref.: #69480]	thermophile	no	95.911	yes
[Ref.: #69480]	aerobic	yes	93.872	no
[Ref.: #69480]	flagellated	no	71.339	no
[Ref.: #69480]	glucose-ferment	no	91.769	yes

Availability in culture collections External links

[Ref.: #21858]

Culture collection no.

DSM 29957, CECT 8399, KCTC 32538

[Ref.: #89288]

SI-ID 393361

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cribrihabitans neustonicus sp. nov., isolated from coastal surface seawater, and emended description of the genus Cribrihabitans Chen et al. 2014.	Hameed A, Shahina M, Lin SY, Lai WA, Liu YC, Hsu YH, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.066142-0	2014	*
Phylogeny	Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring.	Park S, Park DS, Bae KS, Yoon JH	Int J Syst Evol Microbiol	10.1099/ijs.0.057646-0	2014	*
	Draft genome of Leisingera aquaemixtae CECT 8399(T), a member of the Roseobacter clade isolated from a junction of fresh and ocean water in Jeju Island, South Korea.	Rodrigo-Torres L, Pujalte MJ, Arahal DR	Genom Data	10.1016/j.gdata.2016.01.010	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21858

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29957

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#89288

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID393361.1 )

* These data were automatically processed and therefore are not curated

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Leisingera aquaemixtae strain CECT 8399

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