Name: Sphaerisporangium dianthi NEAU-CY18
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130737

Type strain:

Species: Sphaerisporangium dianthi

Strain Designation: NEAU-CY18

Culture col. no.: DSM 46736, CGMCC 4.7132, JCM 33407, NEAU-CY18

Strain history: CGMCC 4.7132 <-- X. Wang and W. Xiang; Northeast Agric. Univ., China; NEAU-CY18.

NCBI tax ID(s): 1436120 (species)

SI-ID 406354

DSM 46736

Section

Sphaerisporangium dianthi NEAU-CY18 is a mesophilic bacterium that builds an aerial mycelium and was isolated from root of the Chinese medicinal plant Dianthus chinensis.

mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Streptosporangiaceae*
	Genus	*Sphaerisporangium*
	Species	**Sphaerisporangium dianthi**
	Full Scientific Name (LPSN)	Sphaerisporangium dianthi Xing et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #69467]	Colony color	Brown beige (1011), light ivory (1015)
[Ref.: #69467]	Cultivation medium used	suter without tyrosine

[Ref.: #69467]	Colony color	Brown beige (1011), oyster white (1013)
[Ref.: #69467]	Cultivation medium used	suter with tyrosine

[Ref.: #69467]	Colony color	Brown beige (1011)
[Ref.: #69467]	Cultivation medium used	ISP 2

[Ref.: #69467]	Colony color	Brown beige (1011)
[Ref.: #69467]	Cultivation medium used	ISP 4

[Ref.: #69467]	Colony color	Brown beige (1011)
[Ref.: #69467]	Cultivation medium used	ISP 7

[Ref.: #69467]	Colony color	Brown beige (1011), deep orange (2011)
[Ref.: #69467]	Cultivation medium used	ISP 3

[Ref.: #69467]	Colony color	Ochre yellow (1024)
[Ref.: #69467]	Cultivation medium used	ISP 6

[Ref.: #69467]	Colony color	Sand yellow (1002), brown beige (1011)
[Ref.: #69467]	Cultivation medium used	ISP 5

[Ref.: #69467]	Pigment production	no
[Ref.: #69467]	Pigment name	Melanin
[Ref.: #69467]	Pigment production	no
[Ref.: #69467]	Pigment name	soluble pigment

[Ref.: #69467]	Multicellular complex forming ability	yes
[Ref.: #69467]	Multicellular complex name	Aerial mycelium
[Ref.: #69467]	Multicellular complex color	Light ivory (1015)
[Ref.: #69467]	Medium Name (multicellularity)	ISP 2

[Ref.: #69467]	Multicellular complex forming ability	yes
[Ref.: #69467]	Multicellular complex name	Aerial mycelium
[Ref.: #69467]	Multicellular complex color	Cream (9001)
[Ref.: #69467]	Medium Name (multicellularity)	ISP 3

[Ref.: #69467]	Multicellular complex forming ability	no
[Ref.: #69467]	Multicellular complex name	Aerial mycelium
[Ref.: #69467]	Medium Name (multicellularity)	ISP 4

[Ref.: #69467]	Multicellular complex forming ability	no
[Ref.: #69467]	Multicellular complex name	Aerial mycelium
[Ref.: #69467]	Medium Name (multicellularity)	ISP 5

[Ref.: #69467]	Multicellular complex forming ability	no
[Ref.: #69467]	Multicellular complex name	Aerial mycelium
[Ref.: #69467]	Medium Name (multicellularity)	ISP 6

[Ref.: #69467]	Multicellular complex forming ability	no
[Ref.: #69467]	Multicellular complex name	Aerial mycelium
[Ref.: #69467]	Medium Name (multicellularity)	ISP 7

[Ref.: #69467]	Multicellular complex forming ability	no
[Ref.: #69467]	Multicellular complex name	Aerial mycelium
[Ref.: #69467]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69467]	Multicellular complex forming ability	no
[Ref.: #69467]	Multicellular complex name	Aerial mycelium
[Ref.: #69467]	Medium Name (multicellularity)	suter without tyrosine

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21833]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #21833]

Culture medium growth

[Ref.: #21833]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21833]

Culture medium composition

expand / minimize

[Ref.: #21833]

Culture medium

ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)

[Ref.: #21833]

Culture medium growth

[Ref.: #21833]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21833]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #21833]			growth	28 °C
[Ref.: #67770]			growth	28 °C

[Ref.: #21833]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69467]		NaCl		growth	0	%

[Ref.: #67770]

Observation

quinones: MK-9(H₄), MK-9(H₂), MK-9(H₆)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69467]	arabinose ChEBI	+	growth
[Ref.: #69467]	cellulose ChEBI	-	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69467]	fructose ChEBI	+	growth
[Ref.: #69467]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69467]	inositol ChEBI	+	growth
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69467]	mannose ChEBI	-	growth
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #69467]	raffinose ChEBI	+	growth
[Ref.: #69467]	rhamnose ChEBI	+/-	growth
[Ref.: #69467]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69467]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API Coryne, API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69467]	3960	-	-	+	+	-	-	+	+	+	-	+/-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69467]	6144	not determinedn.d.	+	+/-	+/-	-	+	+	-	+	+/-	+	+/-	-	+	-	+	+/-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21833]	Sample type/isolated from	root of the Chinese medicinal plant Dianthus chinensis
[Ref.: #21833]	Host species	Dianthus chinensis
[Ref.: #21833]	Geographic location (country and/or sea, region)	Jilin Province, Changchun (43°48'N, 126°36'E)
[Ref.: #21833]	Country	China
[Ref.: #21833]	Country ISO 3 Code	CHN
[Ref.: #21833]	Continent	Asia
[Ref.: #21833]	Geographic location	43.8000°/126.6000°

[Ref.: #67770]	Sample type/isolated from	Root of Dianthus chinensis L. from Changchun
[Ref.: #67770]	Host species	Dianthus chinensis
[Ref.: #67770]	Geographic location (country and/or sea, region)	Jilin Province, northeast China
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KF764483 (>99% sequence identity) for Sphaerisporangium dianthi from Microbeatlas

Aquatic Samples	1
Soil Samples	10
Animal Samples
Plant Samples	12
Total Samples	23

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21833]	Sphaerisporangium dianthi strain NEAU-CY18 16S ribosomal RNA gene, partial sequence	KF764483	1512	ENA	1436120 tax ID

[Ref.: #21833]	GC-content	71.2 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	70.9-71.5 mol%

Availability in culture collections External links

[Ref.: #21833]

Culture collection no.

DSM 46736, CGMCC 4.7132, JCM 33407, NEAU-CY18

[Ref.: #89237]

SI-ID 406354

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Sphaerisporangium corydalis sp. nov., isolated from the root of Corydalis yanhusuo L.	Wang X, Liu C, Cheng J, Zhang Y, Ma Z, Li L, Wang X, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-015-0471-x	2015	*
Phylogeny	Sphaerisporangium dianthi sp. nov., an endophytic actinomycete isolated from a root of Dianthus chinensis L.	Xing J, Liu C, Zhang Y, He H, Zhou Y, Li L, Zhao J, Liu S, Wang X, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-014-0298-x	2014	*
Phylogeny	Sphaerisporangium rhizosphaerae sp. nov., an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell. Arg).	Mu S, Zhao Q, Zhao J, Cao T, Zhao X, Guo X, Li Y, Xiang W, Wang X	Int J Syst Evol Microbiol	10.1099/ijsem.0.002912	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21833

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 46736

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69467

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#89237

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406354.1 )

* These data were automatically processed and therefore are not curated

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