Name: Pseudomonas kilonensis 520-20
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13070

Type strain:

Species: Pseudomonas kilonensis

Strain Designation: 520-20

Culture col. no.: DSM 13647, CFBP 5372, JCM 11939, CIP 107321

Strain history: CIP <- 2001, CFBP <- J. Sikorski, Oldenburg Univ., Oldenburg, Germany

NCBI tax ID(s): 132476 (species)

SI-ID 49503

DSM 13647, CFBP 5372, JCM 11939, CCUG 51510, LMG 21624, KCTC 12937, DSM 13547, NCIMB 13899, CCUG 53881, CIP 107321

Section

Pseudomonas kilonensis 520-20 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from agricultural soil.

Gram-negative
motile
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas kilonensis**
	Full Scientific Name (LPSN)	Pseudomonas kilonensis Sikorski et al. 2001

Information on morphological and physiological properties Morphology

[Ref.: #120413]	Gram stain	negative

[Ref.: #120413]	Cell shape	rod-shaped

[Ref.: #120413]	Motility	yes

[Ref.: #120413]	Pigment production	yes
[Ref.: #120413]	Pigment name	Pyocyanin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4940]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #4940]

Culture medium growth

[Ref.: #4940]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4940]

Culture medium composition

expand / minimize

[Ref.: #4940]

Culture medium

LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)

[Ref.: #4940]

Culture medium growth

[Ref.: #4940]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4940]

Culture medium composition

expand / minimize

[Ref.: #33765]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #33765]

Culture medium growth

[Ref.: #33765]

Culture medium composition

expand / minimize

[Ref.: #120413]

Culture medium

CIP Medium 72

[Ref.: #120413]

Culture medium growth

[Ref.: #120413]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #4940]		growth	28 °C
[Ref.: #33765]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #120413]		growth	5-37 °C
[Ref.: #120413]	no	growth	41 °C

[Ref.: #4940]	Temperature range	mesophilic
[Ref.: #33765]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #120413]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #120413]

Oxygen tolerance

obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #120413]	NaCl		growth	0-2	%
[Ref.: #120413]	NaCl	no	growth	4	%
[Ref.: #120413]	NaCl	no	growth	6	%
[Ref.: #120413]	NaCl	no	growth	8	%
[Ref.: #120413]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #120413]	citrate ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #120413]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #120413]	nitrate ChEBI	+	reduction
[Ref.: #120413]	nitrate ChEBI	+	respiration
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #120413]	nitrite ChEBI	+	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120413]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #120413]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120413]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120413]	amylase	-
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #120413]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120413]	caseinase	-	3.4.21.50
[Ref.: #120413]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #120413]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #120413]	gelatinase	-
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #120413]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #120413]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120413]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120413]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120413]	oxidase	+
[Ref.: #120413]	protease	-
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #120413]	tryptophan deaminase	-
[Ref.: #120413]	tween esterase	+
[Ref.: #120413]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #4940]	22995	+	-	-	-	-	-	+	-	+	+	+	+	-	-	+	+	-	+	+	-	+

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #120413]	2152	not determinedn.d.	+	+	+	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	+	-	-	-	-	+	+	+	-	-	+	-	-	-	-	-	-	+	+	-	+	-	+	+	+	+	-	+	+	+	-	-	-	-	+	-	+	+	+	-	-	-	-	-	-	+	+	+	-	+	+	+	-	+	+	+	-	+	+	-	+	-	+	+	+	+	+	+	+	+	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120413]	10260	-	-	+	+	-	+	+	-	+	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4940]	Sample type/isolated from	agricultural soil
[Ref.: #4940]	Geographic location (country and/or sea, region)	Kiel
[Ref.: #4940]	Country	Germany
[Ref.: #4940]	Country ISO 3 Code	DEU
[Ref.: #4940]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Agricultural soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	near Kiel
[Ref.: #67770]	Country	Germany
[Ref.: #67770]	Country ISO 3 Code	DEU
[Ref.: #67770]	Continent	Europe

[Ref.: #120413]	Sample type/isolated from	Environment, Soil
[Ref.: #120413]	Country	Germany
[Ref.: #120413]	Country ISO 3 Code	DEU
[Ref.: #120413]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	-
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ292426 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	8731
Soil Samples	16904
Animal Samples	11521
Plant Samples	5158
Total Samples	42314

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4940]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120413]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4940]	Pseudomonas kilonensis 16S rRNA gene, strain 520-20	AJ292426	1528	ENA	132476 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Pseudomonas kilonensis DSM 13647	GCA_001269885	contig	GenBank	132476 tax ID
[Ref.: #66792]	Pseudomonas kilonensis strain DSM 13647	132476.6	wgs	PATRIC	132476 tax ID	*
[Ref.: #66792]	Pseudomonas kilonensis DSM 13647	2713897225	draft	JGI IMG/M	132476 tax ID	*
[Ref.: #66792]	Pseudomonas kilonensis DSM 13647	2639762655	draft	JGI IMG/M	132476 tax ID	*
[Ref.: #66792]	Pseudomonas kilonensis DSM 13647	GCA_900105635	contig	GenBank	132476 tax ID	*

[Ref.: #4940]	GC-content	59.9 mol%
[Ref.: #67770]	GC-content	59.9 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas kilonensis strain DSM 13647 PATRIC: 132476.6
[Ref.: #69480]	motile	yes	92.043	no
[Ref.: #69480]	gram-positive	no	98.401	no
[Ref.: #69480]	anaerobic	no	98.483	no
[Ref.: #69480]	halophile	no	91.883	no
[Ref.: #69480]	spore-forming	no	95.744	no
[Ref.: #69480]	glucose-util	yes	94.44	yes
[Ref.: #69480]	aerobic	yes	91.258	no
[Ref.: #69480]	flagellated	yes	85.26	no
[Ref.: #69480]	thermophile	no	99.84	no
[Ref.: #69480]	glucose-ferment	no	88.749	yes

Availability in culture collections External links

[Ref.: #4940]

Culture collection no.

DSM 13647, CFBP 5372, JCM 11939, CIP 107321

[Ref.: #82280]

SI-ID 49503

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas uvaldensis sp. nov., a bacterial pathogen causing onion bulb rot.	Khanal M, Timilsina S, Bhatta BP, Bophela K, Coutinho T, Cochran K, Malla S	Int J Syst Evol Microbiol	10.1099/ijsem.0.005311	2022	*
Phylogeny	Pseudomonas canavaninivorans sp. nov., isolated from bean rhizosphere.	Hauth F, Buck H, Hartig JS	Int J Syst Evol Microbiol	10.1099/ijsem.0.005203	2022	*
Phylogeny	Pseudomonas bijieensis sp. nov., isolated from cornfield soil.	Liang J, Wang S, Yiming A, Fu L, Ahmad I, Chen G, Zhu B	Int J Syst Evol Microbiol	10.1099/ijsem.0.004676	2021	*
Phylogeny	Pseudomonas viciae sp. nov., isolated from rhizosphere of broad bean.	Zhao H, Ma Y, Wu X, Zhang L	Int J Syst Evol Microbiol	10.1099/ijsem.0.004373	2020	*
Phylogeny	Pseudomonas kilonensis sp. nov., a bacterium isolated from agricultural soil.	Sikorski J, Stackebrandt E, Wackernagel W	Int J Syst Evol Microbiol	10.1099/00207713-51-4-1549	2001	*

References

#4940

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13647

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#33765 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#82280

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49503.1 )

#120413 Collection of Institut Pasteur ; Curators of the CIP; CIP 107321
* These data were automatically processed and therefore are not curated

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Pseudomonas kilonensis strain DSM 13647

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