Cite this strain

"Pseudomonas songnenensis" (DSM 27560, NEAU-ST5-5)

BacDive ID 130570

Type strain

Strain Designation NEAU-ST5-5

Culture col. no. DSM 27560 NEAU-ST5-5

NCBI tax ID(s) 1176259

Special Collections DSMZ

Links

"Pseudomonas songnenensis" NEAU-ST5-5 is a mesophilic prokaryote that was isolated from saline-alkaline soil.

mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 407263

DSM 27560

@ref 20215

Last LPSN update 2025-12-16 10:01:01

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Pseudomonas

Species "Pseudomonas songnenensis"

Full scientific name Pseudomonas songnenensis Zhang et al. 2015

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	99.6
125439		91.7
125438		91.003
125438	negative	99.5

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
22020	HIGH MAGNESIUM MEDIUM (DSMZ Medium 1506)		Medium recipe at MediaDive	Name: HIGH MAGNESIUM MEDIUM (DSMZ Medium 1506) Composition: MgSO4 x 7 H2O 20.0 g/l Yeast extract 10.0 g/l Tryptone 5.0 g/l Casein hydrolysate 5.0 g/l Trisodium citrate x 2 H2O 3.0 g/l KCl 2.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
22020	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125438	aerobe	93.419
125439	obligate aerobe	91.3

Spore formation

@ref	Spore formation	Confidence
125439		99.6

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68369	gelatinase	-		from API 20NE
68369	urease	-	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
22020	+	-	-	-	-	-	-	-	+	-	-	-	-	+	+	+	-	+	+	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Condition	#Alkaline	-
#Condition	#Saline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
22020	saline-alkaline soil	Heilongjiang Province, Songnen Plain	China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence JQ762269 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	380
Soil Samples	126
Animal Samples	321
Plant Samples	49
Total Samples	876

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM369631v1 assembly for Pseudomonas songnenensis NEAU-ST5-5	contig	GCA_003696315	1176259.3	8035095626	1176259	74.9

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
22020	Pseudomonas songnenensis strain NEAU-ST5-5 16S ribosomal RNA gene, partial sequence	JQ762269	1458		1176259

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Pseudomonas songnenensis NEAU-ST5-5
NCBI: GCA_003696315

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.60	no
125439	motility	BacteriaNetⓘ	yes	91.70	no
125439	gram_stain	BacteriaNetⓘ	negative	99.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	91.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudomonas songnenensis NEAU-ST5-5
NCBI: GCA_003696315.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.50	no
125438	anaerobic	anaerobicⓘ	no	98.86	no
125438	aerobic	aerobicⓘ	yes	93.42	no
125438	spore-forming	spore-formingⓘ	no	85.66	no
125438	thermophilic	thermophileⓘ	no	100.00	no
125438	flagellated	motile2+ⓘ	yes	91.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas lopnurensis sp. nov., an endophytic bacterium isolated from Populus euphratica at the ancient Ugan river.	Mamtimin T, Anwar N, Abdurahman M, Kurban M, Rozahon M, Mamtimin H, Hamood B, Rahman E, Wu M	Antonie Van Leeuwenhoek	10.1007/s10482-021-01524-8	2021
Phylogeny	Pseudomonas songnenensis sp. nov., isolated from saline and alkaline soils in Songnen Plain, China.	Zhang L, Pan Y, Wang K, Zhang X, Zhang S, Fu X, Zhang C, Jiang J	Antonie Van Leeuwenhoek	10.1007/s10482-014-0365-3	2014

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#22020	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27560
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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"Pseudomonas songnenensis" (DSM 27560, NEAU-ST5-5)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_003696315

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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