Name: Mesorhizobium muleiense DSM 100038
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130530

Type strain:

Species: Mesorhizobium muleiense

Culture col. no.: DSM 100038, CGMCC 1.11022, HAMBI 3264, CCBAU 83963

Strain history: <- HAMBI <- W. F. Chen, China Agric. Univ., College Biol. Sci., Beijing; CCBAU 83963

NCBI tax ID(s): 1004279 (species)

SI-ID 401978

DSM 100038

Section

Mesorhizobium muleiense DSM 100038 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from root nodule of Cicer arietinum.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Phyllobacteriaceae*
	Genus	*Mesorhizobium*
	Species	**Mesorhizobium muleiense**
	Full Scientific Name (LPSN)	Mesorhizobium muleiense Zhang et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #30535]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.972

[Ref.: #30535]	Cell length	1.65 µm

[Ref.: #30535]	Cell width	0.53 µm

[Ref.: #30535]	Cell shape	rod-shaped

[Ref.: #30535]	Motility	yes

[Ref.: #22038]

Incubation period

3-7 days

[Ref.: #30535]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #22038]

Culture medium

RHIZOBIUM MEDIUM (DSMZ Medium 98)

[Ref.: #22038]

Culture medium growth

[Ref.: #22038]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22038]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #22038]			growth	30 °C
[Ref.: #30535]			optimum	28 °C

[Ref.: #22038]	Temperature range	mesophilic
[Ref.: #30535]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30535]			optimum	7
[Ref.: #30535]			growth	06-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30535]

Oxygen tolerance

aerobe

[Ref.: #30535]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.973

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #30535]	arabinose ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #30535]	fructose ChEBI	+	carbon source
[Ref.: #30535]	galactose ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #30535]	glucose ChEBI	+	carbon source
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #30535]	lactose ChEBI	+	carbon source
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #30535]	mannose ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #30535]	proline ChEBI	+	carbon source
[Ref.: #30535]	rhamnose ChEBI	+	carbon source
[Ref.: #30535]	ribose ChEBI	+	carbon source
[Ref.: #30535]	sorbitol ChEBI	+	carbon source
[Ref.: #30535]	sucrose ChEBI	+	carbon source
[Ref.: #30535]	trehalose ChEBI	+	carbon source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #30535]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #22038]	catalase	+	1.11.1.6
[Ref.: #30535]	catalase	+	1.11.1.6
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #22038]	24150	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22038]	Sample type/isolated from	root nodule of Cicer arietinum
[Ref.: #22038]	Host species	Cicer arietinum
[Ref.: #22038]	Geographic location (country and/or sea, region)	Shuangdamen villages, Qitai county, Xinjiang
[Ref.: #22038]	Country	China
[Ref.: #22038]	Country ISO 3 Code	CHN
[Ref.: #22038]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HQ316710 (>99% sequence identity) for Mesorhizobium from Microbeatlas

Aquatic Samples	507
Soil Samples	2635
Animal Samples	282
Plant Samples	1035
Total Samples	4459

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #22038]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #22038]	Mesorhizobium muleiense strain CCBAU 83963 16S ribosomal RNA gene, partial sequence	HQ316710	1474	ENA	1004279 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mesorhizobium muleiense CGMCC 1.11022	GCA_900099905	scaffold	GenBank	1004279 tax ID	*
[Ref.: #66792]	Mesorhizobium muleiense strain CGMCC 1.11022	1004279.8	wgs	PATRIC	1004279 tax ID	*
[Ref.: #66792]	Mesorhizobium muleiense CGMCC 1.11022	2675903064	draft	JGI IMG/M	1004279 tax ID	*

[Ref.: #30535]	GC-content	61.2 mol%
[Ref.: #22038]	GC-content	61.2 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Mesorhizobium muleiense CGMCC 1.11022 NCBI: GCA_900099905.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mesorhizobium muleiense CGMCC 1.11022 NCBI: GCA_900099905.1
[Ref.: #69480]	motile	yes	76.492	yes
[Ref.: #69480]	flagellated	no	91.171	no
[Ref.: #69480]	gram-positive	no	97.832	yes
[Ref.: #69480]	anaerobic	no	99.357	yes
[Ref.: #69480]	aerobic	yes	93.815	yes
[Ref.: #69480]	halophile	no	92.138	no
[Ref.: #69480]	spore-forming	no	94.957	yes
[Ref.: #69480]	glucose-ferment	no	90.304	yes
[Ref.: #69480]	thermophile	no	98.767	yes
[Ref.: #69480]	glucose-util	yes	90.808	no

Availability in culture collections External links

[Ref.: #22038]

Culture collection no.

DSM 100038, CGMCC 1.11022, HAMBI 3264, CCBAU 83963

[Ref.: #89053]

SI-ID 401978

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genotypic alteration and competitive nodulation of Mesorhizobium muleiense against exotic chickpea rhizobia in alkaline soils.	Zhang JJ, Yu T, Lou K, Mao PH, Wang ET, Chen WF, Chen WX	Syst Appl Microbiol	10.1016/j.syapm.2014.07.004	2014	*
Phylogeny	Mesorhizobium muleiense sp. nov., nodulating with Cicer arietinum L.	Zhang JJ, Liu TY, Chen WF, Wang ET, Sui XH, Zhang XX, Li Y, Li Y, Chen WX	Int J Syst Evol Microbiol	10.1099/ijs.0.038265-0	2012	*
	The introduced strain Mesorhizobium ciceri USDA 3378 is more competitive than an indigenous strain in nodulation of chickpea in newly introduced areas of China.	Zhang J, Li S, Wang N, Chen W, Feng X, Jia B, Zhao Y, Yang T, Zong X	Lett Appl Microbiol	10.1111/lam.13785	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22038

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100038

#30535

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26866 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#89053

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401978.1 )

#26866

IJSEM 2737 2012 ( DOI 10.1099/ijs.0.038265-0 , PubMed 22228663 )

* These data were automatically processed and therefore are not curated

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Mesorhizobium muleiense CGMCC 1.11022

Mesorhizobium muleiense CGMCC 1.11022

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