Mesorhizobium muleiense (DSM 100038, CGMCC 1.11022, HAMBI 3264)

Name: Mesorhizobium muleiense (DSM 100038, CGMCC 1.11022, HAMBI 3264)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 130530

Type strain

Culture col. no. DSM 100038 CGMCC 1.11022 HAMBI 3264 CCBAU 83963

NCBI tax ID(s) 1004279

Special Collections DSMZ Literature strains

History DSMZ <- HAMBI <- W. F. Chen, China Agric. Univ., College Biol. Sci., Beijing; CCBAU 83963

Links

Mesorhizobium muleiense DSM 100038 is an aerobe, Gram-negative, motile bacterium that was isolated from root nodule of Cicer arietinum.

Gram-negative motile rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 401978

DSM 100038

@ref 20215

Last LPSN update 2026-05-29 08:37:24

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Bartonellaceae

Genus Mesorhizobium

Species Mesorhizobium muleiense

Full scientific name Mesorhizobium muleiense Zhang et al. 2012

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
30535	negative	1.65 µm	0.53 µm	rod-shaped
125439	negative				98.303
125438	negative				98.5

Colony morphology

22038

Incubation period3-7 days

Pigmentation

30535

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
22038	RHIZOBIUM MEDIUM (DSMZ Medium 98)		Medium recipe at MediaDive	Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
22038	positive	growth	30
30535	positive	optimum	28

Culture pH

@ref	Ability	Type	PH	PH range
30535	positive	optimum	7
30535	positive	growth	06-09	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
30535	aerobe
125439	obligate aerobe	99.516

Spore formation

@ref	Spore formation	Confidence
30535
125439		99.056

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
30535	22599 ChEBI	arabinose	+	carbon source
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
30535	28757 ChEBI	fructose	+	carbon source
30535	28260 ChEBI	galactose	+	carbon source
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
30535	17234 ChEBI	glucose	+	carbon source
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
30535	17716 ChEBI	lactose	+	carbon source
68369	25115 ChEBI	malate	-	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
30535	37684 ChEBI	mannose	+	carbon source
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
30535	26271 ChEBI	proline	+	carbon source
30535	26546 ChEBI	rhamnose	+	carbon source
30535	33942 ChEBI	ribose	+	carbon source
30535	30911 ChEBI	sorbitol	+	carbon source
30535	17992 ChEBI	sucrose	+	carbon source
30535	27082 ChEBI	trehalose	+	carbon source
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
30535	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
22038	catalase	+	1.11.1.6
30535	catalase	+	1.11.1.6
68369	gelatinase	-		from API 20NE
68369	urease	-	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
22038	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
22038	root nodule of Cicer arietinum	Cicer arietinum	Shuangdamen villages, Qitai county, Xinjiang	China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence HQ316710 (>99% sequence identity) for Mesorhizobium from Microbeatlas

Aquatic Samples	507
Soil Samples	2635
Animal Samples	282
Plant Samples	1035
Total Samples	4459

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
22038	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	IMG-taxon 2675903064 annotated assembly for Mesorhizobium muleiense CGMCC 1.11022	scaffold	GCA_900099905	1004279.8	2675903064	1004279	65.91

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
22038	Mesorhizobium muleiense strain CCBAU 83963 16S ribosomal RNA gene, partial sequence	HQ316710	1474		1004279

GC content

@ref	GC-content (mol%)	Method
30535	61.2
22038	61.2	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: IMG-taxon 2675903064 annotated assembly for Mesorhizobium muleiense CGMCC 1.11022
BV-BRC: 1004279.8

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.52	no
125439	gram_stain	BacteriaNetⓘ	negative	98.30	no
125439	spore_formation	BacteriaNetⓘ	no	99.06	no
125439	motility	BacteriaNetⓘ	yes	52.27	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Mesorhizobium muleiense CGMCC 1.11022
NCBI: GCA_900099905.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.50	no
125438	anaerobic	anaerobicⓘ	no	92.76	yes
125438	aerobic	aerobicⓘ	yes	87.48	yes
125438	spore-forming	spore-formingⓘ	no	89.65	yes
125438	thermophilic	thermophileⓘ	no	97.99	no
125438	flagellated	motile2+ⓘ	yes	63.51	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Chickpea: Its Origin, Distribution, Nutrition, Benefits, Breeding, and Symbiotic Relationship with Mesorhizobium Species.	Zhang J, Wang J, Zhu C, Singh RP, Chen W.	Plants (Basel)	10.3390/plants13030429	2024
Phylogeny	Genomic diversity of chickpea-nodulating rhizobia in Ningxia (north central China) and gene flow within symbiotic Mesorhizobium muleiense populations.	Zhang J, Peng S, Shang Y, Brunel B, Li S, Zhao Y, Liu Y, Chen W, Wang E, Singh RP, James EK.	Syst Appl Microbiol	10.1016/j.syapm.2020.126089	2020
	The Effect of Different Rhizobial Symbionts on the Composition and Diversity of Rhizosphere Microorganisms of Chickpea in Different Soils.	Zhang J, Wang N, Li S, Wang J, Feng Y, Wang E, Li Y, Yang T, Chen W.	Plants (Basel)	10.3390/plants12193421	2023
Phylogeny	Evolution and taxonomy of native mesorhizobia nodulating medicinal Glycyrrhiza species in China.	Mousavi SA, Li L, Wei G, Rasanen L, Lindstrom K.	Syst Appl Microbiol	10.1016/j.syapm.2016.03.009	2016
Phylogeny	Genetic diversity and distribution of rhizobia associated with the medicinal legumes Astragalus spp. and Hedysarum polybotrys in agricultural soils.	Yan H, Ji ZJ, Jiao YS, Wang ET, Chen WF, Guo BL, Chen WX.	Syst Appl Microbiol	10.1016/j.syapm.2016.01.004	2016
	A Lipopolysaccharide Synthesis Gene rfaD from Mesorhizobium huakuii Is Involved in Nodule Development and Symbiotic Nitrogen Fixation.	Liu Y, Lin Y, Guan N, Song Y, Li Y, Xie X.	Microorganisms	10.3390/microorganisms11010059	2022
Phylogeny	Diversification of Sinorhizobium populations associated with Medicago polymorpha and Medicago lupulina in purple soil of China.	Tang M, Wang H, Qi X, He T, Zhang B, Wang E, Yu M, Wang B, Wang F, Liu Z, Liu X.	Front Microbiol	10.3389/fmicb.2022.1055694	2022
Genetics	Global-level population genomics reveals differential effects of geography and phylogeny on horizontal gene transfer in soil bacteria.	Greenlon A, Chang PL, Damtew ZM, Muleta A, Carrasquilla-Garcia N, Kim D, Nguyen HP, Suryawanshi V, Krieg CP, Yadav SK, Patel JS, Mukherjee A, Udupa S, Benjelloun I, Thami-Alami I, Yasin M, Patil B, Singh S, Sarma BK, von Wettberg EJB, Kahraman A, Bukun B, Assefa F, Tesfaye K, Fikre A, Cook DR.	Proc Natl Acad Sci U S A	10.1073/pnas.1900056116	2019
Genetics	Analysis of 1,000+ Type-Strain Genomes Substantially Improves Taxonomic Classification of Alphaproteobacteria.	Hordt A, Lopez MG, Meier-Kolthoff JP, Schleuning M, Weinhold LM, Tindall BJ, Gronow S, Kyrpides NC, Woyke T, Goker M.	Front Microbiol	10.3389/fmicb.2020.00468	2020
Genetics	Genotypic alteration and competitive nodulation of Mesorhizobium muleiense against exotic chickpea rhizobia in alkaline soils.	Zhang JJ, Yu T, Lou K, Mao PH, Wang ET, Chen WF, Chen WX	Syst Appl Microbiol	10.1016/j.syapm.2014.07.004	2014
	The introduced strain Mesorhizobium ciceri USDA 3378 is more competitive than an indigenous strain in nodulation of chickpea in newly introduced areas of China.	Zhang J, Li S, Wang N, Chen W, Feng X, Jia B, Zhao Y, Yang T, Zong X	Lett Appl Microbiol	10.1111/lam.13785	2022
Phylogeny	Mesorhizobium wenxiniae sp. nov., isolated from chickpea (Cicer arietinum L.) in China.	Zhang J, Guo C, Chen W, de Lajudie P, Zhang Z, Shang Y, Wang ET.	Int J Syst Evol Microbiol	10.1099/ijsem.0.002770	2018
Phylogeny	Mesorhizobium muleiense sp. nov., nodulating with Cicer arietinum L.	Zhang JJ, Liu TY, Chen WF, Wang ET, Sui XH, Zhang XX, Li Y, Li Y, Chen WX	Int J Syst Evol Microbiol	10.1099/ijs.0.038265-0	2012

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#22038	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100038
#26866	IJSEM 2737 2012 ( DOI 10.1099/ijs.0.038265-0 , PubMed 22228663 )
#30535	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26866
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mesorhizobium muleiense (DSM 100038, CGMCC 1.11022, HAMBI 3264)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900099905

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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