Name: Lactobacillus kullabergensis Biut2N
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130380

Type strain:

Species: Lactobacillus kullabergensis

Strain Designation: Biut2N

Culture col. no.: DSM 26262, CCUG 63631, JCM 30802

Strain history: CCUG 63631 <-- T. C. Olofsson; Lund Univ., Sweden; Biut2N.

NCBI tax ID(s): 1218493 (species)

SI-ID 406632

DSM 26262, CCUG 63631

Other strains from Lactobacillus kullabergensis

L. kullabergensis H6HS21N, DSM 26314, CCUG 63632

Section

Lactobacillus kullabergensis Biut2N is an anaerobe, mesophilic bacterium that was isolated from honey stomach of honey bee .

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Lactobacillus*
	Species	**Lactobacillus kullabergensis**
	Full Scientific Name (LPSN)	Lactobacillus kullabergensis Olofsson et al. 2014

Information on morphological and physiological properties Morphology

[Ref.: #63003]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21916]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #21916]

Culture medium growth

[Ref.: #21916]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21916]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #21916]	growth	30 °C
[Ref.: #63003]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #21916]	Temperature range	mesophilic
[Ref.: #63003]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #21916]	Oxygen tolerance	anaerobe
[Ref.: #63003]	Oxygen tolerance	anaerobe

[Ref.: #21916]	Murein short key	A11.31
[Ref.: #21916]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #21916]	14798	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	+/-	+	+	+	+	+	-	-	-	+	-	-	-	+/-	-	-	+	-	-	+	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21916]	Sample type/isolated from	honey stomach of honey bee (Apis mellifera mellifera)
[Ref.: #21916]	Host species	Apis mellifera mellifera
[Ref.: #21916]	Geographic location (country and/or sea, region)	Lund
[Ref.: #21916]	Country	Sweden
[Ref.: #21916]	Country ISO 3 Code	SWE
[Ref.: #21916]	Continent	Europe

[Ref.: #63003]	Sample type/isolated from	Honey bee,honey stomach
[Ref.: #63003]	Sampling date	2011-07-06
[Ref.: #63003]	Geographic location (country and/or sea, region)	Helsingborg
[Ref.: #63003]	Country	Sweden
[Ref.: #63003]	Country ISO 3 Code	SWE
[Ref.: #63003]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Honey stomach of a honeybee Apis mellifera mellifera
[Ref.: #67770]	Host species	Apis mellifera mellifera
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JX099550 (>99% sequence identity) for Lactobacillus from Microbeatlas

Aquatic Samples	53
Soil Samples	10
Animal Samples	2067
Plant Samples	37
Total Samples	2167

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21916]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21916]	Lactobacillus kullabergensis strain Biut2N 16S ribosomal RNA gene, partial sequence	JX099550	1438	ENA	1218493 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus kullabergensis Biut2	GCA_000967195	scaffold	GenBank	1218493 tax ID

[Ref.: #21916]	GC-content	35.6 mol%	sequence analysis
[Ref.: #67770]	GC-content	35.6 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	35.8 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus kullabergensis Biut2 NCBI: GCA_000967195.1
[Ref.: #69480]	motile	no	96.352	no
[Ref.: #69480]	flagellated	no	98.552	no
[Ref.: #69480]	gram-positive	yes	93.834	no
[Ref.: #69480]	anaerobic	yes	90.66	yes
[Ref.: #69480]	aerobic	no	98.225	yes
[Ref.: #69480]	halophile	yes	79.304	no
[Ref.: #69480]	spore-forming	no	97.384	no
[Ref.: #69480]	thermophile	no	97.811	yes
[Ref.: #69480]	glucose-util	yes	91.619	no
[Ref.: #69480]	glucose-ferment	yes	90.828	no

Availability in culture collections External links

[Ref.: #21916]

Culture collection no.

DSM 26262, CCUG 63631, JCM 30802

[Ref.: #88912]

SI-ID 406632

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera.	Olofsson TC, Alsterfjord M, Nilson B, Butler E, Vasquez A	Int J Syst Evol Microbiol	10.1099/ijs.0.059600-0	2014	*
Phylogeny	Lactobacillus huangpiensis sp. nov. and Lactobacillus laiwuensis sp. nov., isolated from the gut of honeybee (Apis mellifera).	Li TT, Gu CT	Int J Syst Evol Microbiol	10.1099/ijsem.0.005237	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21916

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26262

#63003 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 63631
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#88912

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406632.1 )

* These data were automatically processed and therefore are not curated

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