Name: Lactobacillus kimbladii Hma2N
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130376

Type strain:

Species: Lactobacillus kimbladii

Strain Designation: Hma2N

Culture col. no.: DSM 26263, CCUG 63633, JCM 30767

Strain history: CCUG 63633 <-- T. Olofsson; Lund Univ., Sweden; Hma2N.

NCBI tax ID(s): 1218506 (species)

SI-ID 397595

DSM 26263, CCUG 63633

Other strains from Lactobacillus kimbladii

L. kimbladii H6hs28N, DSM 26318, CCUG 63634

Section

Lactobacillus kimbladii Hma2N is an anaerobe, mesophilic bacterium that was isolated from honey stomach of honey bee .

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Lactobacillus*
	Species	**Lactobacillus kimbladii**
	Full Scientific Name (LPSN)	Lactobacillus kimbladii Olofsson et al. 2014

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %94.777

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21912]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #21912]

Culture medium growth

[Ref.: #21912]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21912]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #21912]	growth	30 °C
[Ref.: #63005]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #21912]	Temperature range	mesophilic
[Ref.: #63005]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #21912]	Oxygen tolerance	anaerobe
[Ref.: #63005]	Oxygen tolerance	anaerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.995

[Ref.: #21912]	Murein short key	A11.31
[Ref.: #21912]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	alpha-cyclodextrin ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	arginine ChEBI	+	hydrolysis	* from API rID32STR
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-mannitol ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	D-ribose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	D-tagatose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	hippurate ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #68381]	L-arabinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	lactose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	maltose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	melezitose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	melibiose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	raffinose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sucrose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	trehalose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	urea ChEBI	-	hydrolysis	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68381]		acetoin ChEBI	yes	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68381]		acetoin ChEBI	+	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	+		* from API rID32STR
[Ref.: #68381]	alkaline phosphatase	-	3.1.3.1	* from API rID32STR
[Ref.: #68381]	alpha-galactosidase	+	3.2.1.22	* from API rID32STR
[Ref.: #68381]	arginine dihydrolase	+	3.5.3.6	* from API rID32STR
[Ref.: #68381]	beta-galactosidase	+	3.2.1.23	* from API rID32STR
[Ref.: #68381]	beta-glucosidase	+	3.2.1.21	* from API rID32STR
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR
[Ref.: #68381]	beta-mannosidase	-	3.2.1.25	* from API rID32STR
[Ref.: #68381]	glycyl tryptophan arylamidase	-		* from API rID32STR
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32STR
[Ref.: #68381]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32STR
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #63005]	5901	+	+	+	-	+	-	+	+	-	+	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21912]	Sample type/isolated from	honey stomach of honey bee (Apis mellifera mellifera)
[Ref.: #21912]	Host species	Apis mellifera mellifera
[Ref.: #21912]	Geographic location (country and/or sea, region)	Lund
[Ref.: #21912]	Country	Sweden
[Ref.: #21912]	Country ISO 3 Code	SWE
[Ref.: #21912]	Continent	Europe

[Ref.: #63005]	Sample type/isolated from	Honey bee,honey stomach
[Ref.: #63005]	Sampling date	2011-07-06
[Ref.: #63005]	Geographic location (country and/or sea, region)	Helsingborg
[Ref.: #63005]	Country	Sweden
[Ref.: #63005]	Country ISO 3 Code	SWE
[Ref.: #63005]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Honey stomach of the honeybee Apis mellifera mellifera
[Ref.: #67770]	Host species	Apis mellifera mellifera
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JX099549 (>99% sequence identity) for Lactobacillus from Microbeatlas

Aquatic Samples	53
Soil Samples	10
Animal Samples	2067
Plant Samples	37
Total Samples	2167

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21912]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21912]	Lactobacillus kimbladii strain Hma2N 16S ribosomal RNA gene, partial sequence	JX099549	1437	ENA	1218506 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus kimbladii Hma2	GCA_000970755	scaffold	GenBank	1218506 tax ID

[Ref.: #21912]	GC-content	35.9 mol%	sequence analysis
[Ref.: #67770]	GC-content	36 mol%
[Ref.: #67770]	GC-content	35.9 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lactobacillus kimbladii Hma2 NCBI: GCA_000970755.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus kimbladii Hma2 NCBI: GCA_000970755.1
[Ref.: #69480]	motile	no	96.43	no
[Ref.: #69480]	flagellated	no	98.757	no
[Ref.: #69480]	gram-positive	yes	94.382	no
[Ref.: #69480]	anaerobic	yes	86.42	yes
[Ref.: #69480]	aerobic	no	97.766	no
[Ref.: #69480]	halophile	yes	77.946	no
[Ref.: #69480]	spore-forming	no	97.421	no
[Ref.: #69480]	thermophile	no	98.648	yes
[Ref.: #69480]	glucose-util	yes	89.658	no
[Ref.: #69480]	glucose-ferment	yes	89.485	no

Availability in culture collections External links

[Ref.: #21912]

Culture collection no.

DSM 26263, CCUG 63633, JCM 30767

[Ref.: #88908]

SI-ID 397595

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera.	Olofsson TC, Alsterfjord M, Nilson B, Butler E, Vasquez A	Int J Syst Evol Microbiol	10.1099/ijs.0.059600-0	2014	*
Phylogeny	Lactobacillus huangpiensis sp. nov. and Lactobacillus laiwuensis sp. nov., isolated from the gut of honeybee (Apis mellifera).	Li TT, Gu CT	Int J Syst Evol Microbiol	10.1099/ijsem.0.005237	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21912

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26263

#63005 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 63633
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68381

Automatically annotated from API rID32STR .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88908

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397595.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Lactobacillus kimbladii in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF