Lactobacillus kimbladii (DSM 26263, CCUG 63633, Hma2N)

Name: Lactobacillus kimbladii (DSM 26263, CCUG 63633, Hma2N)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 130376

Type strain

Strain Designation Hma2N

Culture col. no. DSM 26263 CCUG 63633 Hma2N JCM 30767

NCBI tax ID(s) 1218506

Special Collections DSMZ CCUG JCM

Links

Lactobacillus kimbladii Hma2N is an anaerobe, mesophilic prokaryote that was isolated from honey stomach of honey bee .

anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 397595

LMG 32937,LMG 32397,DSM 26263,CCUG 63633

@ref 20215

Last LPSN update 2025-12-16 10:00:59

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Lactobacillus

Species Lactobacillus kimbladii

Full scientific name Lactobacillus kimbladii Olofsson et al. 2014

BacDive ID	Other strains from **Lactobacillus kimbladii** (1)	Type strain
130377	L. kimbladii H6hs28N, DSM 26318, CCUG 63634

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125438		92.5
125438		92.043	positive

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21912	MRS MEDIUM (DSMZ Medium 11)		Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
21912	positive	growth	30	mesophilic
63005	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
21912	anaerobe
63005	anaerobe
125439	microaerophile	94.1

Spore formation

@ref	Spore formation	Confidence
125439		93.9
125438		91.979

Murein

@ref	Murein short key	Type
21912	A11.31	A4alpha L-Lys-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68381	40585 ChEBI	alpha-cyclodextrin	-	builds acid from	from API rID32STR
68381	29016 ChEBI	arginine	+	hydrolysis	from API rID32STR
68381	18333 ChEBI	D-arabitol	-	builds acid from	from API rID32STR
68381	16899 ChEBI	D-mannitol	+	builds acid from	from API rID32STR
68381	16988 ChEBI	D-ribose	+	builds acid from	from API rID32STR
68381	16443 ChEBI	D-tagatose	-	builds acid from	from API rID32STR
68381	28087 ChEBI	glycogen	-	builds acid from	from API rID32STR
68381	606565 ChEBI	hippurate	-	hydrolysis	from API rID32STR
68381	30849 ChEBI	L-arabinose	-	builds acid from	from API rID32STR
68381	17716 ChEBI	lactose	+	builds acid from	from API rID32STR
68381	17306 ChEBI	maltose	-	builds acid from	from API rID32STR
68381	6731 ChEBI	melezitose	-	builds acid from	from API rID32STR
68381	28053 ChEBI	melibiose	+	builds acid from	from API rID32STR
68381	320055 ChEBI	methyl beta-D-glucopyranoside	-	builds acid from	from API rID32STR
68381	27941 ChEBI	pullulan	-	builds acid from	from API rID32STR
68381	16634 ChEBI	raffinose	+	builds acid from	from API rID32STR
68381	30911 ChEBI	sorbitol	-	builds acid from	from API rID32STR
68381	17992 ChEBI	sucrose	+	builds acid from	from API rID32STR
68381	27082 ChEBI	trehalose	+	builds acid from	from API rID32STR
68381	16199 ChEBI	urea	-	hydrolysis	from API rID32STR

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68381	15688 ChEBI	acetoin		from API rID32STR

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
68381	15688 ChEBI	acetoin	+	from API rID32STR

Enzymes

@ref	Value	Activity	Ec
68381	Alanyl-Phenylalanyl-Proline arylamidase	+		from API rID32STR
68381	alkaline phosphatase	-	3.1.3.1	from API rID32STR
68381	alpha-galactosidase	+	3.2.1.22	from API rID32STR
68381	arginine dihydrolase	+	3.5.3.6	from API rID32STR
68381	beta-galactosidase	+	3.2.1.23	from API rID32STR
68381	beta-glucosidase	+	3.2.1.21	from API rID32STR
68381	beta-glucuronidase	-	3.2.1.31	from API rID32STR
68381	beta-mannosidase	-	3.2.1.25	from API rID32STR
68381	glycyl tryptophan arylamidase	-		from API rID32STR
68381	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32STR
68381	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32STR
68381	urease	-	3.5.1.5	from API rID32STR

API rID32STR

@ref	ADH (Arg)	beta GLU	beta GAR	beta GUR	alpha GAL	PAL	RIB	MAN	SOR	LAC	TRE	RAF	SAC	LARA	DARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	HIP	GLYG	PUL	MAL	MEL	MLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	TAG	beta MAN	URE
63005	+	+	+	-	+	-	+	+	-	+	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	#Stomach

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent	Sampling date
21912	honey stomach of honey bee (Apis mellifera mellifera)	Apis mellifera mellifera	Lund	Sweden	SWE	Europe
63005	Honey bee,honey stomach		Helsingborg	Sweden	SWE	Europe	2011-07-06
67770	Honey stomach of the honeybee Apis mellifera mellifera	Apis mellifera mellifera

Taxonmaps

69479

Global distribution of 16S sequence JX099549 (>99% sequence identity) for Lactobacillus from Microbeatlas

Aquatic Samples	53
Soil Samples	10
Animal Samples	2067
Plant Samples	37
Total Samples	2167

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
21912	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	IMG accession	NCBI tax ID	Score
67770	ASM97075v1 assembly for Lactobacillus kimbladii Hma2	scaffold	GCA_000970755	2627854222	1218506	69.95
124043	ASM4266497v1 assembly for Lactobacillus kimbladii CCUG 63633	scaffold	GCA_042664975		1218506	69.68

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21912	Lactobacillus kimbladii strain Hma2N 16S ribosomal RNA gene, partial sequence	JX099549	1437		1218506

GC content

@ref	GC-content (mol%)	Method
21912	35.9	sequence analysis
67770	36
67770	35.9	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lactobacillus kimbladii Hma2
NCBI: GCA_000970755

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	93.90	no
125439	motility	BacteriaNetⓘ	no	70.40	no
125439	gram_stain	BacteriaNetⓘ	variable	60.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	microaerophile	94.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus kimbladii Hma2
NCBI: GCA_000970755.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.04	no
125438	anaerobic	anaerobicⓘ	no	50.22	yes
125438	aerobic	aerobicⓘ	no	95.83	yes
125438	spore-forming	spore-formingⓘ	no	91.98	no
125438	thermophilic	thermophileⓘ	no	95.06	no
125438	flagellated	motile2+ⓘ	no	92.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera.	Olofsson TC, Alsterfjord M, Nilson B, Butler E, Vasquez A	Int J Syst Evol Microbiol	10.1099/ijs.0.059600-0	2014
Phylogeny	Lactobacillus huangpiensis sp. nov. and Lactobacillus laiwuensis sp. nov., isolated from the gut of honeybee (Apis mellifera).	Li TT, Gu CT	Int J Syst Evol Microbiol	10.1099/ijsem.0.005237	2022

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21912	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26263
#63005	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 63633
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68381	Automatically annotated from API rID32STR .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lactobacillus kimbladii (DSM 26263, CCUG 63633, Hma2N)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Murein

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API rID32STR

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000970755

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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