Bombilactobacillus mellis (DSM 26255, CCUG 63289, Hon2N)

Name: Bombilactobacillus mellis (DSM 26255, CCUG 63289, Hon2N)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 130372

Type strain

Strain Designation Hon2N

Culture col. no. DSM 26255 CCUG 63289 Hon2N JCM 30769

NCBI tax ID(s) 1218508

Special Collections DSMZ CCUG JCM

History <- T. Olofsson, Lund Univ., Sweden; Hon2N CCUG 63289 <-- T. Olofsson; Lund Univ., Sweden; Hon2N.

Links

Bombilactobacillus mellis Hon2N is an anaerobe, mesophilic prokaryote that was isolated from honey stomach of honey bee .

anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 400289

LMG 29584,DSM 26255,CCUG 63289

@ref 20215

Last LPSN update 2026-02-09 11:31:12

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Bombilactobacillus

Species Bombilactobacillus mellis

Full scientific name Bombilactobacillus mellis (Olofsson et al. 2014) Zheng et al. 2020

Synonyms (1)

Lactobacillus mellis

BacDive ID	Other strains from **Bombilactobacillus mellis** (1)	Type strain
130373	B. mellis H1HS38N, DSM 26313, CCUG 63290

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125438		93
125438		93.93	positive

Colony morphology

62965

Incubation period3 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21908	MRS MEDIUM (DSMZ Medium 11)		Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11; with strain-specific modifications) Composition: Fructose 20.0 g/l Glucose 20.0 g/l Meat extract 10.0 g/l Casein peptone 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
21908	positive	growth	30	mesophilic
62965	positive	growth	37	mesophilic
67770	positive	growth	30	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
21908	anaerobe
62965	anaerobe
125439	facultative anaerobe	97.5

Murein

@ref	Murein short key	Type
21908	A11.31	A4alpha L-Lys-D-Asp

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	gluconeogenesis	100	8 of 8
66794	formaldehyde oxidation	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	acetoin degradation	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	ribulose monophosphate pathway	100	2 of 2
66794	ppGpp biosynthesis	100	4 of 4
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	acetate fermentation	100	4 of 4
66794	ceramide biosynthesis	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	palmitate biosynthesis	81.82	18 of 22
66794	pentose phosphate pathway	81.82	9 of 11
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	ketogluconate metabolism	75	6 of 8
66794	peptidoglycan biosynthesis	73.33	11 of 15
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	photosynthesis	71.43	10 of 14
66794	myo-inositol biosynthesis	70	7 of 10
66794	vitamin B1 metabolism	69.23	9 of 13
66794	phenylalanine metabolism	69.23	9 of 13
66794	vitamin B12 metabolism	67.65	23 of 34
66794	serine metabolism	66.67	6 of 9
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	valine metabolism	66.67	6 of 9
66794	chorismate metabolism	66.67	6 of 9
66794	glycolysis	64.71	11 of 17
66794	d-xylose degradation	63.64	7 of 11
66794	NAD metabolism	61.11	11 of 18
66794	glycogen metabolism	60	3 of 5
66794	cellulose degradation	60	3 of 5
66794	methylglyoxal degradation	60	3 of 5
66794	Entner Doudoroff pathway	60	6 of 10
66794	pyrimidine metabolism	60	27 of 45
66794	threonine metabolism	60	6 of 10
66794	propanol degradation	57.14	4 of 7
66794	ubiquinone biosynthesis	57.14	4 of 7
66794	degradation of sugar alcohols	56.25	9 of 16
66794	purine metabolism	55.32	52 of 94
66794	non-pathway related	55.26	21 of 38
66794	degradation of pentoses	53.57	15 of 28
66794	ethanol fermentation	50	1 of 2
66794	isoleucine metabolism	50	4 of 8
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	suberin monomers biosynthesis	50	1 of 2
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	quinate degradation	50	1 of 2
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	adipate degradation	50	1 of 2
66794	butanoate fermentation	50	2 of 4
66794	glutamate and glutamine metabolism	50	14 of 28
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	sulfopterin metabolism	50	2 of 4
66794	oxidative phosphorylation	48.35	44 of 91
66794	vitamin B6 metabolism	45.45	5 of 11
66794	degradation of hexoses	44.44	8 of 18
66794	d-mannose degradation	44.44	4 of 9
66794	mevalonate metabolism	42.86	3 of 7
66794	heme metabolism	42.86	6 of 14
66794	isoprenoid biosynthesis	42.31	11 of 26
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	alanine metabolism	41.38	12 of 29
66794	lysine metabolism	40.48	17 of 42
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	lipoate biosynthesis	40	2 of 5
66794	methionine metabolism	38.46	10 of 26
66794	arginine metabolism	37.5	9 of 24
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	37.5	3 of 8
66794	degradation of sugar acids	36	9 of 25
66794	tryptophan metabolism	34.21	13 of 38
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	octane oxidation	33.33	1 of 3
66794	lipid A biosynthesis	33.33	3 of 9
66794	sphingosine metabolism	33.33	2 of 6
66794	CO2 fixation in Crenarchaeota	33.33	3 of 9
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	cyanate degradation	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	lipid metabolism	32.26	10 of 31
66794	ascorbate metabolism	31.82	7 of 22
66794	urea cycle	30.77	4 of 13
66794	starch degradation	30	3 of 10
66794	tyrosine metabolism	28.57	4 of 14
66794	tetrahydrofolate metabolism	28.57	4 of 14
66794	citric acid cycle	28.57	4 of 14
66794	cysteine metabolism	27.78	5 of 18
66794	metabolism of disaccharids	27.27	3 of 11
66794	proline metabolism	27.27	3 of 11
66794	flavin biosynthesis	26.67	4 of 15
66794	cyclohexanol degradation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	androgen and estrogen metabolism	25	4 of 16
66794	lactate fermentation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	CMP-KDO biosynthesis	25	1 of 4
66794	biotin biosynthesis	25	1 of 4
66794	glycogen biosynthesis	25	1 of 4
66794	histidine metabolism	24.14	7 of 29
66794	leucine metabolism	23.08	3 of 13
66794	sulfate reduction	23.08	3 of 13
66794	nitrate assimilation	22.22	2 of 9
66794	polyamine pathway	21.74	5 of 23

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	#Stomach

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent	Sampling date
21908	honey stomach of honey bee (Apis mellifera mellifera)	Apis mellifera mellifera	Lund	Sweden	SWE	Europe
62965	Honey bee,honey stomach		Helsingborg	Sweden	SWE	Europe	2011-07-06
67770	Honey stomach of the honeybee Apis mellifera mellifera	Apis mellifera mellifera

Taxonmaps

69479

Global distribution of 16S sequence JX099545 (>99% sequence identity) for Bombilactobacillus mellis subclade from Microbeatlas

Aquatic Samples	685
Soil Samples	322
Animal Samples	9931
Plant Samples	460
Total Samples	11398

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
21908	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	IMG accession	NCBI tax ID	Score
124043	ASM4267640v1 assembly for Bombilactobacillus mellis CCUG 63289	contig	GCA_042676405		1218508	76.01
67770	ASM96724v1 assembly for Bombilactobacillus mellis Hon2	scaffold	GCA_000967245	2639763010	1218508	75.51

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21908	Lactobacillus mellis strain Hon2N 16S ribosomal RNA gene, partial sequence	JX099545	1435		1218508

GC content

@ref	GC-content (mol%)	Method
21908	36.4	sequence analysis
67770	36.4	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Bombilactobacillus mellis Hon2
NCBI: GCA_000967245

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	87.00	no
125439	motility	BacteriaNetⓘ	no	62.00	no
125439	gram_stain	BacteriaNetⓘ	positive	73.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	97.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Bombilactobacillus mellis Hon2
NCBI: GCA_000967245.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	93.93	no
125438	anaerobic	anaerobicⓘ	no	53.39	yes
125438	aerobic	aerobicⓘ	no	96.49	yes
125438	spore-forming	spore-formingⓘ	no	80.42	no
125438	thermophilic	thermophileⓘ	no	92.45	no
125438	flagellated	motile2+ⓘ	no	93.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Type VI secretion systems promote intraspecific competition and host interactions in a bee gut symbiont.	Motta EVS, Lariviere PJ, Jones KR, Song Y, Moran NA.	Proc Natl Acad Sci U S A	10.1073/pnas.2414882121	2024
Phylogeny	Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera.	Olofsson TC, Alsterfjord M, Nilson B, Butler E, Vasquez A	Int J Syst Evol Microbiol	10.1099/ijs.0.059600-0	2014

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21908	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26255
#62965	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 63289
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Bombilactobacillus mellis (DSM 26255, CCUG 63289, Hon2N)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Murein

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_042676405

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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