Name: Pseudactinotalea suaedae DSM 28005
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130241

Type strain:

Species: Pseudactinotalea suaedae

Culture col. no.: DSM 28005, JCM 19624, KACC 17839, KCTC 29256, EGI 60002

Strain history: <- Wenjun Li, China

NCBI tax ID(s): 1524924 (species)

SI-ID 396670

DSM 28005

Other strains from Pseudactinotalea suaedae

P. suaedae DSM 28006, EGI 60003
P. suaedae DSM 28007, EGI 60004

Section

Pseudactinotalea suaedae DSM 28005 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from surface-sterilized healthy plant material of the halophyte Suaeda physophora.

colony-forming
Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Cellulomonadaceae*
	Genus	*Pseudactinotalea*
	Species	**Pseudactinotalea suaedae**
	Full Scientific Name (LPSN)	Pseudactinotalea suaedae (Zhao et al. 2015) Cho et al. 2017

Synonym

Actinotalea suaedae

Information on morphological and physiological properties Morphology

[Ref.: #25230]	Gram stain	negative
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #25230]	Cell shape	rod-shaped

[Ref.: #25230]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %94.132

[Ref.: #25230]	Colony color	yellow
[Ref.: #25230]	Colony shape	circular
[Ref.: #25230]	Incubation period	4 days
[Ref.: #25230]	Cultivation medium used	TSA

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25230]

Culture medium

Trypticase Soy Agar (TSA)

[Ref.: #25230]

Culture medium growth

[Ref.: #25230]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #25230]

Culture medium growth

[Ref.: #25230]

Culture medium

LB (Luria-Bertani) MEDIUM

[Ref.: #25230]

Culture medium growth

[Ref.: #22046]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #22046]

Culture medium growth

[Ref.: #22046]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22046]

Culture medium composition

expand / minimize

[Ref.: #25230]

Culture medium

MacConkey agar

[Ref.: #25230]

Culture medium growth

		Temperature
[Ref.: #22046]	growth	28 °C
[Ref.: #25230]	growth	15-35 °C
[Ref.: #25230]	optimum	28 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	28 °C

[Ref.: #22046]	Temperature range	mesophilic
[Ref.: #25230]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #25230]			optimum	7
[Ref.: #25230]			growth	6.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25230]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.967

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25230]		NaCl		growth	0.0-5.0	%(w/v)
[Ref.: #25230]		NaCl		optimum	3	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-10(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25230]	3-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #25230]	3-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #25230]	4-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #25230]	adipate ChEBI	-	assimilation
[Ref.: #25230]	cellulose ChEBI	+	hydrolysis
[Ref.: #25230]	D-arabinose ChEBI	-	builds acid from
[Ref.: #25230]	D-fructose ChEBI	+	builds acid from
[Ref.: #25230]	D-galactose ChEBI	+	builds acid from
[Ref.: #25230]	D-glucose ChEBI	+	builds acid from
[Ref.: #25230]	D-mannitol ChEBI	+	builds acid from
[Ref.: #25230]	D-mannose ChEBI	+	builds acid from
[Ref.: #25230]	D-ribose ChEBI	+	assimilation
[Ref.: #25230]	D-sorbitol ChEBI	-	assimilation
[Ref.: #25230]	D-xylose ChEBI	+	builds acid from
[Ref.: #25230]	decanoate ChEBI	-	assimilation
[Ref.: #25230]	dextrin ChEBI	+	hydrolysis
[Ref.: #25230]	dna ChEBI	-	hydrolysis
[Ref.: #25230]	esculin ChEBI	+	hydrolysis
[Ref.: #25230]	gelatin ChEBI	+	hydrolysis
[Ref.: #25230]	glucose ChEBI	-	fermentation
[Ref.: #25230]	glycogen ChEBI	-	assimilation
[Ref.: #25230]	itaconate ChEBI	-	assimilation
[Ref.: #25230]	L-alanine ChEBI	-	assimilation
[Ref.: #25230]	L-arabinose ChEBI	+	builds acid from
[Ref.: #25230]	L-fucose ChEBI	+	assimilation
[Ref.: #25230]	L-histidine ChEBI	-	assimilation
[Ref.: #25230]	L-proline ChEBI	-	assimilation
[Ref.: #25230]	L-rhamnose ChEBI	+	assimilation
[Ref.: #25230]	L-serine ChEBI	-	assimilation
[Ref.: #25230]	L-sorbose ChEBI	-	builds acid from
[Ref.: #25230]	lactate ChEBI	-	assimilation
[Ref.: #25230]	lactose ChEBI	-	builds acid from
[Ref.: #25230]	malate ChEBI	-	assimilation
[Ref.: #25230]	maltose ChEBI	+	builds acid from
[Ref.: #25230]	melibiose ChEBI	+	assimilation
[Ref.: #25230]	myo-inositol ChEBI	-	assimilation
[Ref.: #25230]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #25230]	nitrate ChEBI	+	reduction
[Ref.: #25230]	pectin ChEBI	+	hydrolysis
[Ref.: #25230]	phenylacetate ChEBI	-	assimilation
[Ref.: #25230]	potassium 2-dehydro-D-gluconate	+	assimilation
[Ref.: #25230]	potassium 5-dehydro-D-gluconate	-	assimilation
[Ref.: #25230]	potassium gluconate ChEBI	+	assimilation
[Ref.: #25230]	propionate ChEBI	-	assimilation
[Ref.: #25230]	salicin ChEBI	+	assimilation
[Ref.: #25230]	sodium acetate ChEBI	-	assimilation
[Ref.: #25230]	sodium citrate ChEBI	-	assimilation
[Ref.: #25230]	sodium malonate ChEBI	-	assimilation
[Ref.: #25230]	starch ChEBI	+	hydrolysis
[Ref.: #25230]	suberic acid ChEBI	-	assimilation
[Ref.: #25230]	sucrose ChEBI	+	builds acid from
[Ref.: #25230]	tween 80 ChEBI	-	hydrolysis
[Ref.: #25230]	valerate ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #25230]	amikacin ChEBI				yes	30	µg
[Ref.: #25230]	ampicillin ChEBI	yes	10	µg
[Ref.: #25230]	cefazolin ChEBI	yes	30	µg
[Ref.: #25230]	cefoperazone ChEBI				yes	75	µg
[Ref.: #25230]	ceftriaxone ChEBI				yes	30	µg
[Ref.: #25230]	cefuroxime ChEBI				yes	30	µg
[Ref.: #25230]	chloramphenicol ChEBI				yes	30	µg
[Ref.: #25230]	ciprofloxacin ChEBI	yes	5	µg
[Ref.: #25230]	clindamycin ChEBI	yes	2	µg
[Ref.: #25230]	erythromycin ChEBI	yes	15	µg
[Ref.: #25230]	gentamicin ChEBI				yes	10	µg
[Ref.: #25230]	norfloxacin ChEBI	yes	10	µg
[Ref.: #25230]	ofloxacin ChEBI	yes	5	µg
[Ref.: #25230]	penicillin ChEBI	yes	10	Unit
[Ref.: #25230]	piperacillin ChEBI				yes	100	µg
[Ref.: #25230]	polymyxin b ChEBI				yes	300	Unit
[Ref.: #25230]	sulfamethoxazole ChEBI	yes	1.25	µg
[Ref.: #25230]	vancomycin ChEBI				yes	30	µg
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #25230]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #25230]	acid phosphatase	-	3.1.3.2
[Ref.: #25230]	alkaline phosphatase	-	3.1.3.1
[Ref.: #25230]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #25230]	alpha-fucosidase	-	3.2.1.51
[Ref.: #25230]	alpha-galactosidase	-	3.2.1.22
[Ref.: #25230]	alpha-glucosidase	+	3.2.1.20
[Ref.: #25230]	alpha-mannosidase	+	3.2.1.24
[Ref.: #25230]	arginine dihydrolase	+	3.5.3.6
[Ref.: #25230]	beta-galactosidase	+	3.2.1.23
[Ref.: #25230]	beta-glucosidase	+	3.2.1.21
[Ref.: #25230]	beta-glucuronidase	-	3.2.1.31
[Ref.: #25230]	cystine arylamidase	-	3.4.11.3
[Ref.: #25230]	cytochrome oxidase	-	1.9.3.1
[Ref.: #25230]	esterase (C 4)	+
[Ref.: #25230]	esterase Lipase (C 8)	+
[Ref.: #25230]	leucine arylamidase	+	3.4.11.1
[Ref.: #25230]	lipase (C 14)	-
[Ref.: #25230]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #25230]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #25230]	trypsin	-	3.4.21.4
[Ref.: #25230]	urease	-	3.5.1.5
[Ref.: #25230]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #25230]

Fatty acid	Percentage	ECL
C15:0 anteiso	66.3
C14:0 iso	7.5
C15:0 iso	7.2
C14:0	6.6
C15:1 anteiso A	3.1
C16:0	2
Unknown	2	19
C17:0 anteiso	1.7

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22046]	Sample type/isolated from	surface-sterilized healthy plant material (stems and roots) of the halophyte Suaeda physophora
[Ref.: #22046]	Host species	Suaeda physophora
[Ref.: #22046]	Geographic location (country and/or sea, region)	Xinjiang, near the Fukang Field Research Station of the Chinese Academy of Sciences (44°17' N 87°55' E)
[Ref.: #22046]	Country	China
[Ref.: #22046]	Country ISO 3 Code	CHN
[Ref.: #22046]	Continent	Asia
[Ref.: #22046]	Geographic location	44.2833°/87.9167°

[Ref.: #25230]	Sample type/isolated from	Suaeda physophora grown in saline areas
[Ref.: #25230]	Geographic location (country and/or sea, region)	Fukang City, Xinjiang Province, Northwest China
[Ref.: #25230]	Country	China
[Ref.: #25230]	Country ISO 3 Code	CHN
[Ref.: #25230]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Halophyte Suaeda physophora grown on saline areas of Fukang City
[Ref.: #67770]	Host species	Suaeda physophora
[Ref.: #67770]	Geographic location (country and/or sea, region)	Xinjiang Province, northwest China
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From Glycyrrhiza
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Condition	#Saline	-
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)
#Host Body-Site	#Plant	#Stem (Branch)
#Host Body-Site	#Plant	#Sterilized plant part

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KJ769194 (>99% sequence identity) for Cellulomonadaceae from Microbeatlas

Aquatic Samples	398
Soil Samples	388
Animal Samples	641
Plant Samples	145
Total Samples	1572

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #22046]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #22046]	Actinotalea suaedae strain EGI 60002 16S ribosomal RNA gene, partial sequence	KJ769194	1563	ENA	1524924 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Pseudactinotalea suaedae KACC 17839	GCA_009733815	contig	GenBank	1524924 tax ID
[Ref.: #66792]	Pseudactinotalea suaedae strain KACC 17839	1524924.3	wgs	PATRIC	1524924 tax ID	*
[Ref.: #66792]	Pseudactinotalea suaedae KACC 17839	2887398948	draft	JGI IMG/M	1524924 tax ID	*

[Ref.: #25230]	GC-content	72.3 mol%
[Ref.: #22046]	GC-content	72.3 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pseudactinotalea suaedae strain KACC 17839 PATRIC: 1524924.3
[Ref.: #69481]	spore-forming	no	77	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudactinotalea suaedae strain KACC 17839 PATRIC: 1524924.3
[Ref.: #69480]	motile	no	92.314	yes
[Ref.: #69480]	gram-positive	yes	78.837	yes
[Ref.: #69480]	anaerobic	no	99.467	yes
[Ref.: #69480]	aerobic	yes	94.099	yes
[Ref.: #69480]	halophile	no	90.233	no
[Ref.: #69480]	spore-forming	no	91.78	no
[Ref.: #69480]	glucose-util	yes	87.231	no
[Ref.: #69480]	flagellated	no	98.811	no
[Ref.: #69480]	thermophile	no	98.572	yes
[Ref.: #69480]	glucose-ferment	no	88.456	yes

Availability in culture collections External links

[Ref.: #22046]

Culture collection no.

DSM 28005, JCM 19624, KACC 17839, KCTC 29256, EGI 60002

[Ref.: #88783]

SI-ID 396670

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinotalea caeni sp. nov., isolated from a sludge sample of a biofilm reactor.	Jin L, Ko SR, Lee CS, Ahn CY, Lee JS, Lee KC, Oh HM, Lee HG	Int J Syst Evol Microbiol	10.1099/ijsem.0.001769	2017	*
Phylogeny	Pseudactinotalea terrae gen. nov., sp. nov., isolated from greenhouse soil, and reclassification of Actinotalea suaedae as Pseudactinotalea suaedae comb. nov.	Cho H, Hamada M, Ahn JH, Weon HY, Joa JH, Suzuki KI, Kwon SW, Kim SJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001701	2017	*
Phylogeny	Actinotalea suaedae sp. nov., isolated from the halophyte Suaeda physophora in Xinjiang, Northwest China.	Zhao S, Li L, Li SH, Wang HF, Hozzein WN, Zhang YG, Wadaan MA, Li WJ, Tian CY	Antonie Van Leeuwenhoek	10.1007/s10482-014-0297-y	2014	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22046

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28005

#25230

H. H. Cho, M.,Ahn, J. H.,Weon, H. Y.,Joa, J. H.,Suzuki, K. I.,Kwon, S. W.,Kim, S. J.: Pseudactinotalea terrae gen. nov., sp. nov., isolated from greenhouse soil, and reclassification of Actinotalea suaedae as Pseudactinotalea suaedae comb. nov. IJSEM 67: 704 - 709 2017 ( DOI 10.1099/ijsem.0.001701 , PubMed 27902298 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88783

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID396670.1 )

* These data were automatically processed and therefore are not curated

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Pseudactinotalea suaedae strain KACC 17839

Pseudactinotalea suaedae strain KACC 17839

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