Name: Paraburkholderia aspalathi VG1C
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130231

Type strain:

Species: Paraburkholderia aspalathi

Strain Designation: VG1C

Culture col. no.: DSM 27239, LMG 27731

Strain history: <- N. Mavengere, Dept. Botany and Zoology, Stellenbosch Univ., South Africa

NCBI tax ID(s): 1324617 (species)

SI-ID 390418

LMG 27731, DSM 27239, DSM 27731

Section

Paraburkholderia aspalathi VG1C is a mesophilic, motile bacterium that was isolated from root nodules of Aspalathus abietina Thunb..

motile
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Paraburkholderia*
	Species	**Paraburkholderia aspalathi**
	Full Scientific Name (LPSN)	Paraburkholderia aspalathi (Mavengere et al. 2014) Sawana et al. 2017

Synonym

Burkholderia aspalathi

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.95

[Ref.: #69480]	Motility	yes Confidence in %91.392

[Ref.: #21542]	Type of hemolysis	gamma
[Ref.: #21542]	Incubation period	2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21542]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #21542]

Culture medium growth

[Ref.: #21542]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21542]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #21542]			growth	30 °C

[Ref.: #21542]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %98
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.996

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #21542]	catalase	+	1.11.1.6
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #21542]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #21542]	23971	-	-	-	-	-	-	-	+	+	+/-	+	+	+	-	+	+	+	+	+	+	not determinedn.d.
[Ref.: #21542]	25436	-	-	-	-	-	-	-	+	+	+/-	+	+	+	-	+	+	+	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21542]	Sample type/isolated from	root nodules of Aspalathus abietina Thunb.
[Ref.: #21542]	Host species	Aspalathus abietina
[Ref.: #21542]	Geographic location (country and/or sea, region)	Western Cape Province, Helderberg area, Vergelegen Wine Estate (34.05462° S 18.9367° E)
[Ref.: #21542]	Country	South Africa
[Ref.: #21542]	Country ISO 3 Code	ZAF
[Ref.: #21542]	Continent	Africa
[Ref.: #21542]	Geographic location	-34.0546°/18.9367°
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21542]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21542]	Burkholderia aspalathi strain VG1C 16S ribosomal RNA gene, partial sequence	KC817488	1409	ENA	1324617 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Paraburkholderia aspalathi LMG 27731	GCA_900116445	scaffold	GenBank	1324617 tax ID	*
[Ref.: #66792]	Paraburkholderia aspalathi strain LMG 27731	1324617.3	wgs	PATRIC	1324617 tax ID	*
[Ref.: #66792]	Paraburkholderia aspalathi LMG 27731	2671180117	draft	JGI IMG/M	1324617 tax ID	*

[Ref.: #21542]

GC-content

60.1 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Paraburkholderia aspalathi LMG 27731 NCBI: GCA_900116445.1
[Ref.: #69481]	spore-forming	no	98	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Paraburkholderia aspalathi LMG 27731 NCBI: GCA_900116445.1
[Ref.: #69480]	motile	yes	84.449	no
[Ref.: #69480]	flagellated	no	87.873	no
[Ref.: #69480]	gram-positive	no	98.007	no
[Ref.: #69480]	anaerobic	no	98.603	no
[Ref.: #69480]	aerobic	yes	86.728	no
[Ref.: #69480]	halophile	no	95.206	no
[Ref.: #69480]	spore-forming	no	93.743	no
[Ref.: #69480]	glucose-ferment	no	90.313	yes
[Ref.: #69480]	thermophile	no	99.666	no
[Ref.: #69480]	glucose-util	yes	90.581	yes

Availability in culture collections External links

[Ref.: #21542]

Culture collection no.

DSM 27239, LMG 27731

[Ref.: #88773]

SI-ID 390418

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paraburkholderia madseniana sp. nov., a phenolic acid-degrading bacterium isolated from acidic forest soil.	Wilhelm RC, Murphy SJL, Feriancek NM, Karasz DC, DeRito CM, Newman JD, Buckley DH	Int J Syst Evol Microbiol	10.1099/ijsem.0.004029	2020	*
Phylogeny	Paraburkholderia caffeinilytica sp. nov., isolated from the soil of a tea plantation.	Gao Z, Yuan Y, Xu L, Liu R, Chen M, Zhang C	Int J Syst Evol Microbiol	10.1099/ijsem.0.001333	2016	*
Phylogeny	Burkholderia aspalathi sp. nov., isolated from root nodules of the South African legume Aspalathus abietina Thunb.	Mavengere NR, Ellis AG, Le Roux JJ	Int J Syst Evol Microbiol	10.1099/ijs.0.057067-0	2014	*
Phylogeny	Paraburkholderia solitsugae sp. nov. and Paraburkholderia elongata sp. nov., phenolic acid-degrading bacteria isolated from forest soil and emended description of Paraburkholderia madseniana.	Wilhelm RC, Cyle KT, Martinez CE, Karasz DC, Newman JD, Buckley DH	Int J Syst Evol Microbiol	10.1099/ijsem.0.004387	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21542

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27239

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88773

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID390418.1 )

* These data were automatically processed and therefore are not curated

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Paraburkholderia aspalathi LMG 27731

Paraburkholderia aspalathi LMG 27731

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