Melghiribacillus thermohalophilus (DSM 25894, CCUG 62543, Nari2A)

Name: Melghiribacillus thermohalophilus (DSM 25894, CCUG 62543, Nari2A)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 130213

Type strain

Strain Designation Nari2A

Culture col. no. DSM 25894 CCUG 62543 Nari2A

NCBI tax ID(s) 1324956

Special Collections DSMZ CCUG

History DSMZ <- M. L. Fardeau, Lab. Microbiol. de l'IRD/ESIL, Univ. de Provence et de la Méditerranée, Marseille, France; Nari2A <- N. A. Addou, Inst. of Biol. Sciences, Algiers, Algeria (USTHB)

Links

Melghiribacillus thermohalophilus Nari2A is an aerobe bacterium that was isolated from soil of a salt lake.

aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 399282

DSM 25894,CCUG 62543

@ref 20215

Last LPSN update 2026-05-29 08:37:19

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Melghiribacillus

Species Melghiribacillus thermohalophilus

Full scientific name Melghiribacillus thermohalophilus Addou et al. 2015

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
22152	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)		Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
22152	positive	growth	55
62856	positive	growth	56

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
62856	aerobe
125439	obligate aerobe	93.126

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	cellulose degradation	100	5 of 5
66794	suberin monomers biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	aspartate and asparagine metabolism	100	9 of 9
66794	adipate degradation	100	2 of 2
66794	denitrification	100	2 of 2
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	teichoic acid biosynthesis	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	coenzyme A metabolism	100	4 of 4
66794	threonine metabolism	100	10 of 10
66794	formaldehyde oxidation	100	3 of 3
66794	propanol degradation	100	7 of 7
66794	folate polyglutamylation	100	1 of 1
66794	vitamin K metabolism	100	5 of 5
66794	metabolism of amino sugars and derivatives	100	5 of 5
66794	lipoate biosynthesis	100	5 of 5
66794	palmitate biosynthesis	95.45	21 of 22
66794	starch degradation	90	9 of 10
66794	propionate fermentation	90	9 of 10
66794	valine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	gluconeogenesis	87.5	7 of 8
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	leucine metabolism	84.62	11 of 13
66794	phenylalanine metabolism	84.62	11 of 13
66794	selenocysteine biosynthesis	83.33	5 of 6
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	peptidoglycan biosynthesis	80	12 of 15
66794	phenylacetate degradation (aerobic)	80	4 of 5
66794	photosynthesis	78.57	11 of 14
66794	citric acid cycle	78.57	11 of 14
66794	serine metabolism	77.78	7 of 9
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	biotin biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	glycogen biosynthesis	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	ppGpp biosynthesis	75	3 of 4
66794	flavin biosynthesis	73.33	11 of 15
66794	NAD metabolism	72.22	13 of 18
66794	glycolysis	70.59	12 of 17
66794	alanine metabolism	68.97	20 of 29
66794	pyrimidine metabolism	68.89	31 of 45
66794	tryptophan metabolism	68.42	26 of 38
66794	purine metabolism	67.02	63 of 94
66794	acetoin degradation	66.67	2 of 3
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	lysine metabolism	66.67	28 of 42
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	histidine metabolism	65.52	19 of 29
66794	heme metabolism	64.29	9 of 14
66794	d-xylose degradation	63.64	7 of 11
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	arginine metabolism	62.5	15 of 24
66794	oxidative phosphorylation	60.44	55 of 91
66794	glycine betaine biosynthesis	60	3 of 5
66794	factor 420 biosynthesis	60	3 of 5
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	tetrahydrofolate metabolism	57.14	8 of 14
66794	d-mannose degradation	55.56	5 of 9
66794	cysteine metabolism	55.56	10 of 18
66794	non-pathway related	55.26	21 of 38
66794	pentose phosphate pathway	54.55	6 of 11
66794	proline metabolism	54.55	6 of 11
66794	methionine metabolism	53.85	14 of 26
66794	urea cycle	53.85	7 of 13
66794	lipid metabolism	51.61	16 of 31
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	CMP-KDO biosynthesis	50	2 of 4
66794	ethanol fermentation	50	1 of 2
66794	acetate fermentation	50	2 of 4
66794	Entner Doudoroff pathway	50	5 of 10
66794	pantothenate biosynthesis	50	3 of 6
66794	sulfopterin metabolism	50	2 of 4
66794	glycine metabolism	50	5 of 10
66794	butanoate fermentation	50	2 of 4
66794	ketogluconate metabolism	50	4 of 8
66794	polyamine pathway	47.83	11 of 23
66794	degradation of pentoses	46.43	13 of 28
66794	ginsenoside metabolism	43.75	7 of 16
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	tyrosine metabolism	42.86	6 of 14
66794	cardiolipin biosynthesis	42.86	3 of 7
66794	glutathione metabolism	42.86	6 of 14
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	methylglyoxal degradation	40	2 of 5
66794	coenzyme M biosynthesis	40	4 of 10
66794	glycogen metabolism	40	2 of 5
66794	sulfate reduction	38.46	5 of 13
66794	phosphatidylethanolamine bioynthesis	38.46	5 of 13
66794	cholesterol biosynthesis	36.36	4 of 11
66794	vitamin B6 metabolism	36.36	4 of 11
66794	arachidonic acid metabolism	33.33	6 of 18
66794	lipid A biosynthesis	33.33	3 of 9
66794	cyanate degradation	33.33	1 of 3
66794	molybdenum cofactor biosynthesis	33.33	3 of 9
66794	IAA biosynthesis	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	nitrate assimilation	33.33	3 of 9
66794	enterobactin biosynthesis	33.33	1 of 3
66794	octane oxidation	33.33	1 of 3
66794	androgen and estrogen metabolism	31.25	5 of 16
66794	degradation of sugar alcohols	31.25	5 of 16
66794	phenylpropanoid biosynthesis	30.77	4 of 13
66794	4-hydroxyphenylacetate degradation	30	3 of 10
66794	bile acid biosynthesis, neutral pathway	29.41	5 of 17
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	reductive acetyl coenzyme A pathway	28.57	2 of 7
66794	degradation of hexoses	27.78	5 of 18
66794	metabolism of disaccharids	27.27	3 of 11
66794	ascorbate metabolism	27.27	6 of 22
66794	catecholamine biosynthesis	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	toluene degradation	25	1 of 4

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Terrestrial	#Soil
#Condition	#Saline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Sampling date
22152	soil of a salt lake	Saharan region, Chott Melghir salt lake located north-east of Biskra city (34° 00' 00''–34° 30' 01'' N 6° 07' 30''–6° 30' 02'' E)	Algeria	DZA	Africa	34.25	6.25 34.25/6.25
62856	Soil,salt lake	Biskra,Chott Melghir (salt lake)	Algeria	DZA	Africa			2006

Taxonmaps

69479

Global distribution of 16S sequence KC845574 (>99% sequence identity) for Melghiribacillus thermohalophilus subclade from Microbeatlas

Aquatic Samples	175
Soil Samples	214
Animal Samples	136
Plant Samples	56
Total Samples	581

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
22152	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM434290v1 assembly for Melghiribacillus thermohalophilus DSM 25894	scaffold	GCA_004342905	1324956.3	2784746804	1324956	67.93

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
22152	Melghiribacillus thermohalophilus strain Nari2A 16S ribosomal RNA gene, partial sequence	KC845574	1552		1324956

GC content

@ref	GC-content (mol%)	Method
22152	41.9	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM434290v1 assembly for Melghiribacillus thermohalophilus DSM 25894
NCBI: GCA_004342905

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	93.13	no
125439	motility	BacteriaNetⓘ	yes	77.42	no
125439	gram_stain	BacteriaNetⓘ	variable	74.19	no
125439	spore_formation	BacteriaNetⓘ	yes	84.96	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Melghiribacillus thermohalophilus DSM 25894
NCBI: GCA_004342905.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	80.65	no
125438	anaerobic	anaerobicⓘ	no	88.21	yes
125438	aerobic	aerobicⓘ	yes	79.03	yes
125438	spore-forming	spore-formingⓘ	yes	86.50	no
125438	thermophilic	thermophileⓘ	yes	51.73	yes
125438	flagellated	motile2+ⓘ	yes	78.58	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Purification and biochemical characterization of a new organic solvent-tolerant chitinase from Paenibacillus timonensis strain LK-DZ15 isolated from the Djurdjura Mountains in Kabylia, Algeria.	Yahiaoui M, Laribi-Habchi H, Bouacem K, Asmani KL, Mechri S, Harir M, Bendif H, Aissani-El Fertas R, Jaouadi B	Carbohydr Res	10.1016/j.carres.2019.107747	2019
Enzymology	Purification and biochemical characterization of a novel thermostable and halotolerant subtilisin SAPN, a serine protease from Melghiribacillus thermohalophilus Nari2A(T) for chitin extraction from crab and shrimp shell by-products.	Mechri S, Bouacem K, Jabeur F, Mohamed S, Addou NA, Dab A, Bouraoui A, Bouanane-Darenfed A, Bejar S, Hacene H, Baciou L, Lederer F, Jaouadi B	Extremophiles	10.1007/s00792-019-01105-8	2019
Enzymology	Purification and biochemical characterization of a novel acido-halotolerant and thermostable endochitinase from Melghiribacillus thermohalophilus strain Nari2A(T).	Mohamed S, Bouacem K, Mechri S, Addou NA, Laribi-Habchi H, Fardeau ML, Jaouadi B, Bouanane-Darenfed A, Hacene H	Carbohydr Res	10.1016/j.carres.2018.12.017	2018
Phylogeny	Melghiribacillus thermohalophilus gen. nov., sp. nov., a novel filamentous, endospore-forming, thermophilic and halophilic bacterium.	Addou NA, Schumann P, Sproer C, Ben Hania W, Hacene H, Fauque G, Cayol JL, Fardeau ML	Int J Syst Evol Microbiol	10.1099/ijs.0.000075	2015

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#22152	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25894
#62856	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 62543
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Melghiribacillus thermohalophilus (DSM 25894, CCUG 62543, Nari2A)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_004342905

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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