Name: Halobacillus andaensis NEAU-ST10-40
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130201

Type strain:

Species: Halobacillus andaensis

Strain Designation: NEAU-ST10-40

Culture col. no.: DSM 25866, CGMCC 1.12153

Strain history: <- J. Juquan, Northeast Agricultural Univ., Harbin, China; NEAU-ST10-40

NCBI tax ID(s): 1176239 (species)

SI-ID 401587

DSM 25866

Section

Halobacillus andaensis NEAU-ST10-40 is an obligate aerobe, moderately halophilic, chemoorganotroph bacterium that forms circular colonies and was isolated from saline, alkaline soil.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
chemoorganotroph
moderately halophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Halobacillus*
	Species	**Halobacillus andaensis**
	Full Scientific Name (LPSN)	Halobacillus andaensis Wang et al. 2015

Information on morphological and physiological properties Morphology

[Ref.: #22994]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #22994]	Cell length	1.5-2.2 µm

[Ref.: #22994]	Cell width	0.6-0.8 µm

[Ref.: #22994]	Cell shape	rod-shaped

[Ref.: #22994]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %98.671

[Ref.: #22994]	Flagellum arrangement	peritrichous

[Ref.: #22994]	Colony size	2.0-3.5 mm
[Ref.: #22994]	Colony color	light yellow
[Ref.: #22994]	Colony shape	circular
[Ref.: #22994]	Incubation period	3 days
[Ref.: #22994]	Cultivation medium used	8% (w/v) NaCl modifed S-G agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #22994]

Culture medium

8% (w/v) NaCl modified S-G agar

[Ref.: #22994]

Culture medium growth

[Ref.: #21602]

Culture medium

PAYNE, SEGHAL & GIBBONS MEDIUM (DSMZ Medium 1160)

[Ref.: #21602]

Culture medium growth

[Ref.: #21602]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21602]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #22994]			growth	4.0-60.0 °C
[Ref.: #22994]			optimum	30.0 °C

[Ref.: #22994]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #22994]			optimum	8.0
[Ref.: #22994]			growth	7.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #22994]

Oxygen tolerance

obligate aerobe

[Ref.: #22994]

Nutrition type

chemoorganotroph

[Ref.: #22994]	Spore description	ellipsoidal, central or subterminal,in swollen sporangia
[Ref.: #22994]	Type of spore	endospore
[Ref.: #22994]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.624

[Ref.: #22994]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #22994]		NaCl		growth	3.0-15.0	%(w/v)
[Ref.: #22994]		NaCl		optimum	8.0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #22994]	amygdalin ChEBI	-	builds acid from
[Ref.: #22994]	casein	-	hydrolysis
[Ref.: #22994]	cellobiose ChEBI	+	builds acid from
[Ref.: #22994]	D-fructose ChEBI	+	builds acid from
[Ref.: #22994]	D-galactose ChEBI	-	builds acid from
[Ref.: #22994]	D-glucose ChEBI	-	builds acid from
[Ref.: #22994]	D-glucose ChEBI	-	fermentation
[Ref.: #22994]	D-glucose ChEBI	-	oxidation
[Ref.: #22994]	D-mannitol ChEBI	-	builds acid from
[Ref.: #22994]	D-mannose ChEBI	+	builds acid from
[Ref.: #22994]	D-xylose ChEBI	+	builds acid from
[Ref.: #22994]	ethanol ChEBI	-	builds acid from
[Ref.: #22994]	gelatin ChEBI	-	hydrolysis
[Ref.: #22994]	glucose ChEBI	-	fermentation
[Ref.: #22994]	glycerol ChEBI	+	builds acid from
[Ref.: #22994]	L-arabinose ChEBI	-	builds acid from
[Ref.: #22994]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #22994]	lactose ChEBI	-	builds acid from
[Ref.: #22994]	maltose ChEBI	-	builds acid from
[Ref.: #22994]	myo-inositol ChEBI	-	builds acid from
[Ref.: #22994]	ribitol ChEBI	-	builds acid from
[Ref.: #22994]	ribose ChEBI	+	builds acid from
[Ref.: #22994]	sorbitol ChEBI	-	builds acid from
[Ref.: #22994]	starch ChEBI	-	hydrolysis
[Ref.: #22994]	sucrose ChEBI	-	builds acid from
[Ref.: #22994]	trehalose ChEBI	+	builds acid from
[Ref.: #22994]	tween 20 ChEBI	+	hydrolysis
[Ref.: #22994]	tween 80 ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #22994]		acetoin ChEBI	no
[Ref.: #22994]		indole ChEBI	yes

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #22994]		acetoin ChEBI	-
[Ref.: #22994]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #22994]	arginine dihydrolase	-	3.5.3.6
[Ref.: #22994]	beta-galactosidase	+	3.2.1.23
[Ref.: #22994]	catalase	+	1.11.1.6
[Ref.: #22994]	cytochrome oxidase	+	1.9.3.1
[Ref.: #22994]	lysine decarboxylase	-	4.1.1.18
[Ref.: #22994]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #22994]	phenylalanine deaminase	-	4.3.1.5
[Ref.: #22994]	tryptophan deaminase	-	4.1.99.1
[Ref.: #22994]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21602]	Sample type/isolated from	saline, alkaline soil
[Ref.: #21602]	Geographic location (country and/or sea, region)	Heilongjiang Province, Anda City (46° 05' 08.83'' N 125° 51' 58.37'' E)
[Ref.: #21602]	Country	China
[Ref.: #21602]	Country ISO 3 Code	CHN
[Ref.: #21602]	Continent	Asia
[Ref.: #21602]	Geographic location	46.0858°/125.8660°

[Ref.: #22994]	Enrichment culture	5 % (w/v) NaCl modified S-G liquid medium
[Ref.: #22994]	Enrichment culture composition	per litre:10 g tryptone,5 g yeast extract,5 g casein,2 g KCl,3 g sodium citrate,20 g MgSO4 .7H2O and 50 g NaCl (pH 7.2-7.4)
[Ref.: #22994]	Enrichment culture duration	2-3 days
[Ref.: #22994]	Enrichment culture temperature	28 ̊C
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Condition	#Alkaline	-
#Condition	#Saline	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21602]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21602]	Halobacillus andaensis strain NEAU-ST10-40 16S ribosomal RNA gene, partial sequence	JQ762290	1437	ENA	1176239 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Halobacillus andaensis CGMCC 1.12153	GCA_014636475	contig	GenBank	1176239 tax ID	*
[Ref.: #66792]	Halobacillus andaensis DSM 25866	GCA_017874135	contig	GenBank	1176239 tax ID	*
[Ref.: #66792]	Halobacillus andaensis strain CGMCC 1.12153	1176239.3	wgs	PATRIC	1176239 tax ID	*
[Ref.: #66792]	Halobacillus andaensis strain DSM 25866	1176239.4	wgs	PATRIC	1176239 tax ID	*
[Ref.: #66792]	Halobacillus andaensis DSM 25866	2913453739	draft	JGI IMG/M	1176239 tax ID	*

[Ref.: #21602]

GC-content

39.3 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Halobacillus andaensis strain DSM 25866 PATRIC: 1176239.4
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Halobacillus andaensis strain CGMCC 1.12153 PATRIC: 1176239.3
[Ref.: #69480]	motile	yes	88.876	yes
[Ref.: #69480]	gram-positive	yes	88.67	yes
[Ref.: #69480]	anaerobic	no	97.913	yes
[Ref.: #69480]	aerobic	yes	92.444	yes
[Ref.: #69480]	halophile	yes	92.671	yes
[Ref.: #69480]	spore-forming	yes	93.62	yes
[Ref.: #69480]	flagellated	yes	83.298	no
[Ref.: #69480]	thermophile	no	98.958	yes
[Ref.: #69480]	glucose-util	yes	89.807	no
[Ref.: #69480]	glucose-ferment	no	90.175	yes

Availability in culture collections External links

[Ref.: #21602]

Culture collection no.

DSM 25866, CGMCC 1.12153

[Ref.: #88747]

SI-ID 401587

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil.	Wang K, Zhang L, Yang Y, Pan Y, Meng L, Liu H, Hong S, Huang H, Jiang J	Int J Syst Evol Microbiol	10.1099/ijs.0.000198	2015	*
Metabolism	A UPF0118 family protein with uncharacterized function from the moderate halophile Halobacillus andaensis represents a novel class of Na(+)(Li(+))/H(+) antiporter.	Dong P, Wang L, Song N, Yang L, Chen J, Yan M, Chen H, Zhang R, Li J, Abdel-Motaal H, Jiang J	Sci Rep	10.1038/srep45936	2017	*
	Characterization of a Functionally Unknown Arginine-Aspartate-Aspartate Family Protein From Halobacillus andaensis and Functional Analysis of Its Conserved Arginine/Aspartate Residues.	Shao L, Abdel-Motaal H, Chen J, Chen H, Xu T, Meng L, Zhang Z, Meng F, Jiang J	Front Microbiol	10.3389/fmicb.2018.00807	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21602

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25866

#22994

Kaibiao Wang,Lei Zhang,Yang Yang,Yuanyuan Pan,Lin Meng,Henan Liu,Shan Hong,Haipeng Huang,Juquan Jiang: Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil. IJSEM 65: 1908 - 1914 2015 ( DOI 10.1099/ijs.0.000198 , PubMed 25795064 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88747

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401587.1 )

* These data were automatically processed and therefore are not curated

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Halobacillus andaensis strain DSM 25866

Halobacillus andaensis strain CGMCC 1.12153

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