Name: Actinotignum sanguinis IMMIBL-2199
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130140

Type strain:

Species: Actinotignum sanguinis

Strain Designation: IMMIBL-2199

Culture col. no.: DSM 26039, CCUG 64068

Strain history: <- A. F. Yassin, Inst. Med. Microbiology, Univ. Bonn, Germany; IMMIBL-2199

NCBI tax ID(s): 1445614 (species)

SI-ID 405978

DSM 26039, CCUG 64068

Other strains from Actinotignum sanguinis

A. sanguinis CCUG 56690
A. sanguinis CCUG 69831
A. sanguinis CCUG 71099

Section

Actinotignum sanguinis IMMIBL-2199 is an anaerobe, mesophilic bacterium that was isolated from blood of a human patient with septicaemia.

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Actinomycetales*
	Family	*Actinomycetaceae*
	Genus	*Actinotignum*
	Species	**Actinotignum sanguinis**
	Full Scientific Name (LPSN)	Actinotignum sanguinis Yassin et al. 2015

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %93.994

[Ref.: #63072]

Incubation period

3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #22143]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #22143]

Culture medium growth

[Ref.: #22143]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22143]

Culture medium composition

expand / minimize

[Ref.: #22143]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #22143]

Culture medium growth

[Ref.: #22143]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22143]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #22143]			growth	37 °C
[Ref.: #63072]			growth	37 °C

[Ref.: #22143]	Temperature range	mesophilic
[Ref.: #63072]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #22143]	Oxygen tolerance	anaerobe
[Ref.: #63072]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %95.478

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68379]	D-glucose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68379]	D-ribose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne, API rID32A
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne, API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A, API Coryne
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	-	3.2.1.23	* from API rID32A, API Coryne
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A, API Coryne
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A, API Coryne
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	+		* from API rID32A
[Ref.: #68380]	leucine arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A, API Coryne
[Ref.: #68380]	proline-arylamidase	+	3.4.11.5	* from API rID32A
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	+		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A, API Coryne
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #22143]	4271	-	-	+/-	+/-	-	-	+	-	-	-	-	-	+	+	+/-	-	+	-	+	-	not determinedn.d.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #22143]	13169	-	-	-	-	-	+	-	-	-	-	-	+/-	-	-	-	-	-	+	+	-	+/-	+	-	+	-	+/-	+/-	-	+
[Ref.: #22143]	12802	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	+	-	+	-	+/-	+/-	-	+/-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22143]	Sample type/isolated from	blood of a human patient with septicaemia
[Ref.: #22143]	Geographic location (country and/or sea, region)	Ingolstadt
[Ref.: #22143]	Country	Germany
[Ref.: #22143]	Country ISO 3 Code	DEU
[Ref.: #22143]	Continent	Europe
[Ref.: #22143]	Geographic location	48.7665°/11.4258°

[Ref.: #63072]	Sample type/isolated from	Human blood
[Ref.: #63072]	Sampling date	2002-11-01
[Ref.: #63072]	Geographic location (country and/or sea, region)	Ingollstadt
[Ref.: #63072]	Country	Germany
[Ref.: #63072]	Country ISO 3 Code	DEU
[Ref.: #63072]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Infection	#Patient	-
#Host Body Product	#Fluids	#Blood

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HG798952 (>99% sequence identity) for Actinotignum sanguinis from Microbeatlas

Aquatic Samples	16
Soil Samples	19
Animal Samples	1278
Plant Samples
Total Samples	1313

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #22143]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #22143]	Actinotignum sanguinis partial 16S rRNA gene, type strain IMMIB L-2199T	HG798952	1508	ENA	1445614 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinotignum sanguinis DSM 26039	GCA_003957255	contig	GenBank	1445614 tax ID	*
[Ref.: #66792]	Actinotignum sanguinis strain DSM 26039	1445614.3	wgs	PATRIC	1445614 tax ID	*
[Ref.: #66792]	Actinotignum sanguinis DSM 26039	2866239221	draft	JGI IMG/M	1445614 tax ID	*

[Ref.: #22143]

GC-content

61.1 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinotignum sanguinis strain DSM 26039 PATRIC: 1445614.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinotignum sanguinis strain DSM 26039 PATRIC: 1445614.3
[Ref.: #69480]	motile	no	94.599	no
[Ref.: #69480]	gram-positive	yes	92.344	no
[Ref.: #69480]	anaerobic	yes	79.046	yes
[Ref.: #69480]	halophile	no	70.84	no
[Ref.: #69480]	spore-forming	no	96.919	no
[Ref.: #69480]	glucose-util	yes	89.156	no
[Ref.: #69480]	thermophile	no	93.911	yes
[Ref.: #69480]	aerobic	no	93.219	yes
[Ref.: #69480]	flagellated	no	97.33	no
[Ref.: #69480]	glucose-ferment	yes	74.38	no

Availability in culture collections External links

[Ref.: #22143]

Culture collection no.

DSM 26039, CCUG 64068

[Ref.: #88694]

SI-ID 405978

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Dissection of the genus Actinobaculum: Reclassification of Actinobaculum schaalii Lawson et al. 1997 and Actinobaculum urinale Hall et al. 2003 as Actinotignum schaalii gen. nov., comb. nov. and Actinotignum urinale comb. nov., description of Actinotignum sanguinis sp. nov. and emended descriptions of the genus Actinobaculum and Actinobaculum suis; and re-examination of the culture deposited as Actinobaculum massiliense CCUG 47753T ( = DSM 19118T), revealing that it does not represent a strain of this species.	Yassin AF, Sproer C, Pukall R, Sylvester M, Siering C, Schumann P	Int J Syst Evol Microbiol	10.1099/ijs.0.069294-0	2014	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22143

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26039

#63072 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 64068

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68379

Automatically annotated from API Coryne .

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88694

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405978.1 )

* These data were automatically processed and therefore are not curated

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Actinotignum sanguinis strain DSM 26039

Actinotignum sanguinis strain DSM 26039

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