Name: Alkalihalophilus marmarensis GMBAE72
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1300

Type strain:

Species: Alkalihalophilus marmarensis

Strain Designation: GMBAE72

Culture col. no.: DSM 21297, JCM 15719, GMBE 72

Strain history: A. A. Denizci GMBE 72.

NCBI tax ID(s): 521377 (species)

SI-ID 404195

DSM 21297

Section

Alkalihalophilus marmarensis GMBAE72 is an aerobe, spore-forming, mesophilic bacterium that was isolated from mushroom compost.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Alkalihalophilus*
	Species	**Alkalihalophilus marmarensis**
	Full Scientific Name (LPSN)	Alkalihalophilus marmarensis (Denizci et al. 2010) Joshi et al. 2022

	Synonym	Bacillus marmarensis
	Synonym	Alkalihalobacillus marmarensis

Information on morphological and physiological properties Morphology

[Ref.: #29401]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #29401]	Cell length	2-2.5 µm

[Ref.: #29401]	Cell width	0.8-1.1 µm

[Ref.: #29401]	Cell shape	rod-shaped

[Ref.: #29401]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %96.953

[Ref.: #29401]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15609]

Culture medium

ALKALINE NUTRIENT AGAR (DSMZ Medium 31)

[Ref.: #15609]

Culture medium growth

[Ref.: #15609]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15609]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #15609]	growth	30 °C
[Ref.: #29401]	growth	15-45 °C
[Ref.: #29401]	optimum	35 °C
[Ref.: #67770]	growth	35 °C

[Ref.: #15609]	Temperature range	mesophilic
[Ref.: #29401]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29401]			optimum	10
[Ref.: #29401]			growth	8-12.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29401]

Oxygen tolerance

aerobe

[Ref.: #29401]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #67770]

Name of produced compound

Protease

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29401]		NaCl		growth	0-12	%
[Ref.: #29401]		NaCl		optimum	0-5	%

[Ref.: #29401]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29401]	cellobiose ChEBI	+	carbon source
[Ref.: #29401]	mannose ChEBI	+	carbon source
[Ref.: #29401]	ribose ChEBI	+	carbon source
[Ref.: #29401]	tween 80 ChEBI	+	carbon source

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #29401]		catalase	+	1.11.1.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15609]	Sample type/isolated from	mushroom compost
[Ref.: #15609]	Geographic location (country and/or sea, region)	Marmara region
[Ref.: #15609]	Country	Turkey
[Ref.: #15609]	Country ISO 3 Code	TUR

[Ref.: #67770]	Sample type/isolated from	Mushroom compost from Yalova
[Ref.: #67770]	Geographic location (country and/or sea, region)	Marmara region
[Ref.: #67770]	Country	Turkey
[Ref.: #67770]	Country ISO 3 Code	TUR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Composting
#Host	#Fungi	#Mushroom
#Engineered	#Agriculture	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15609]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15609]	Bacillus marmarensis strain GMBE72 16S ribosomal RNA gene, partial sequence	EU621902	1309	ENA	1188261 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Alkalihalophilus marmarensis DSM 21297	GCA_000474275	contig	GenBank	1188261 tax ID
[Ref.: #66792]	Bacillus marmarensis DSM 21297	1188261.3	wgs	PATRIC	1188261 tax ID	*
[Ref.: #66792]	Bacillus marmarensis DSM 21297	2563367048	draft	JGI IMG/M	1188261 tax ID	*

[Ref.: #15609]	GC-content	40.2 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29401]	GC-content	40.2 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bacillus marmarensis DSM 21297 PATRIC: 1188261.3
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus marmarensis DSM 21297 PATRIC: 1188261.3
[Ref.: #69480]	motile	yes	91.129	yes
[Ref.: #69480]	gram-positive	yes	90.078	yes
[Ref.: #69480]	anaerobic	no	97.214	no
[Ref.: #69480]	aerobic	yes	89.323	yes
[Ref.: #69480]	halophile	yes	64.111	no
[Ref.: #69480]	spore-forming	yes	96.222	no
[Ref.: #69480]	flagellated	yes	84.576	no
[Ref.: #69480]	glucose-util	yes	91.372	no
[Ref.: #69480]	thermophile	no	99.008	no
[Ref.: #69480]	glucose-ferment	no	93.897	no

Availability in culture collections External links

[Ref.: #15609]

Culture collection no.

DSM 21297, JCM 15719, GMBE 72

[Ref.: #70951]

SI-ID 404195

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Physiology	Investigation of the Physiology of the Obligate Alkaliphilic Bacillus marmarensis GMBE 72(T) Considering Its Alkaline Adaptation Mechanism for Poly(3-hydroxybutyrate) Synthesis.	Atakav Y, Pinar O, Kazan D	Microorganisms	10.3390/microorganisms9020462	2021	*
Transcriptome	What Are the Multi-Omics Mechanisms for Adaptation by Microorganisms to High Alkalinity? A Transcriptomic and Proteomic Study of a Bacillus Strain with Industrial Potential.	Altinisik Kaya FE, Avci FG, Sayar NA, Kazan D, Sayar AA, Sariyar Akbulut B	OMICS	10.1089/omi.2018.0127	2018	*
Metabolism	Assessment of poly(3-hydroxybutyrate) synthesis from a novel obligate alkaliphilic Bacillus marmarensis and generation of its composite scaffold via electrospinning.	Ozgoren T, Pinar O, Bozdag G, Denizci AA, Gunduz O, Cakir Hatir P, Kazan D	Int J Biol Macromol	10.1016/j.ijbiomac.2018.08.014	2018	*
Phylogeny	Bacillus lindianensis sp. nov., a novel alkaliphilic and moderately halotolerant bacterium isolated from saline and alkaline soils.	Dou G, Liu H, He W, Ma Y	Antonie Van Leeuwenhoek	10.1007/s10482-015-0616-y	2015	*
Genetics	Genome Sequence of the Extreme Obligate Alkaliphile Bacillus marmarensis Strain DSM 21297.	Wernick DG, Choi KY, Tat CA, Lafontaine Rivera JG, Liao JC	Genome Announc	10.1128/genomeA.00967-13	2013	*
Phylogeny	Bacillus marmarensis sp. nov., an alkaliphilic, protease-producing bacterium isolated from mushroom compost.	Denizci AA, Kazan D, Erarslan A	Int J Syst Evol Microbiol	10.1099/ijs.0.012369-0	2009	*

References

#15609

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21297

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29401

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25808 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70951

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404195.1 )

#25808

IJSEM 1590 2010 ( DOI 10.1099/ijs.0.012369-0 , PubMed 19700450 )

* These data were automatically processed and therefore are not curated

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Bacillus marmarensis DSM 21297

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