Name: Pseudomonas fragi DSM 3456
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12861

Type strain:

Species: Pseudomonas fragi

Culture col. no.: DSM 3456, ATCC 4973, NRRL B-25, WDCM 00116

Strain history: <- ATCC <- B.W. Hammer

NCBI tax ID(s): 296 (species)

Other strains from Pseudomonas fragi

P. fragi STI09548(IMET), AzIV, IMET 09689
P. fragi STI09555(IMET), T-V,
P. fragi CIP 60.46, LMG 5919
P. fragi CIP 60.47, LMG 5920
P. fragi CIP 60.48
P. fragi CIP 60.49
P. fragi CCUG 45
P. fragi CCUG 159
P. fragi CCUG 1206
P. fragi CCUG 2073, NRRL B-2397
P. fragi CCUG 18759
P. fragi CCUG 29524
P. fragi CCUG 54802
P. fragi CCUG 59953
P. fragi CCUG 59954
P. fragi S117, DSM 108137
P. fragi JCM 20237, IAM 1650, IFO 12049, ...
P. fragi JCM 5362
P. fragi JCM 5363
P. fragi JCM 5364
P. fragi JCM 5365
P. fragi JCM 5366
P. fragi JCM 5367
P. fragi JCM 5368
P. fragi JCM 5369
P. fragi JCM 5370
P. fragi JCM 5371
P. fragi JCM 5372
P. fragi JCM 5373
P. fragi JCM 5374
P. fragi JCM 5375
P. fragi JCM 5376
P. fragi JCM 5377
P. fragi JCM 5378
P. fragi JCM 5379
P. fragi JCM 5380
P. fragi JCM 5382
P. fragi JCM 5383
P. fragi JCM 5384
P. fragi JCM 5385
P. fragi JCM 5386
P. fragi JCM 5387
P. fragi JCM 5388
P. fragi JCM 5389
P. fragi JCM 5390
P. fragi JCM 5391
P. fragi JCM 5392
P. fragi JCM 5393
P. fragi JCM 5394
P. fragi JCM 5395
P. fragi JCM 5396
P. fragi JCM 5397
P. fragi JCM 5398
P. fragi JCM 5399
P. fragi JCM 5400
P. fragi JCM 5401
P. fragi JCM 5402
P. fragi JCM 5403
P. fragi JCM 5404
P. fragi JCM 5407
P. fragi JCM 5408
P. fragi JCM 5409
P. fragi JCM 5410
P. fragi JCM 5411
P. fragi JCM 5412
P. fragi JCM 5413
P. fragi JCM 5414
P. fragi JCM 5417
P. fragi JCM 5418
P. fragi JCM 5419
P. fragi JCM 5420
P. fragi JCM 5421
P. fragi JCM 5422
P. fragi JCM 5423
P. fragi JCM 5425
P. fragi JCM 5426
P. fragi JCM 5427
P. fragi JCM 5428
P. fragi JCM 5429
P. fragi JCM 5430
P. fragi JCM 5431
P. fragi JCM 5432
P. fragi JCM 5433
P. fragi JCM 5434
P. fragi JCM 5435
P. fragi JCM 5436
P. fragi JCM 5437
P. fragi JCM 5438
P. fragi JCM 5440
P. fragi JCM 5441
P. fragi JCM 5442
P. fragi JCM 5444
P. fragi JCM 5445
P. fragi JCM 5446
P. fragi JCM 5447
P. fragi JCM 5448
P. fragi JCM 5449
P. fragi JCM 5451
P. fragi JCM 5452
P. fragi JCM 5453
P. fragi JCM 5454
P. fragi JCM 5456
P. fragi JCM 5457
P. fragi JCM 5460
P. fragi JCM 5461
P. fragi JCM 5462
P. fragi JCM 5464
P. fragi JCM 5467
P. fragi JCM 5468
P. fragi JCM 5469
P. fragi JCM 5472
P. fragi JCM 5473
P. fragi JCM 5474
P. fragi JCM 5475
P. fragi JCM 5476
P. fragi JCM 5477
P. fragi JCM 5479
P. fragi JCM 5483

Section

Pseudomonas fragi DSM 3456 is a mesophilic bacterium of the family Pseudomonadaceae.

mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas fragi**
	Full Scientific Name (PNU)	Pseudomonas fragi (Eichholz 1902) Gruber 1905 (Approved Lists 1980)

Synonym

"Bacterium fragi"

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1314]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #1314]

Culture medium growth

[Ref.: #1314]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #1314]

Culture medium composition

expand / minimize

[Ref.: #1314]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #1314]

Culture medium growth

[Ref.: #1314]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #1314]

Culture medium composition

expand / minimize

[Ref.: #36579]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #36579]

Culture medium growth

[Ref.: #36579]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #1314]			growth	26 °C
[Ref.: #36579]			growth	30 °C

[Ref.: #1314]	Temperature range	mesophilic
[Ref.: #36579]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #68369]		indole ChEBI	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #1314]	22412	-	-	-	+	-	-	-	-	+	+	-	-	-	-	+	+	-	+	+	-	+
[Ref.: #1314]	22006	-	-	-	+	-	-	-	-	+	+	-	-	-	-	+	+	-	+	+	-	+
[Ref.: #1314]	20209	-	-	-	+	-	-	-	-	+	+	-	-	-	-	+	+	-	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #69479]

Global distribution of 16S sequence AB680088 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	830
Soil Samples	460
Animal Samples	1081
Plant Samples	571
Total Samples	2942

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1314]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Pseudomonas fragi strain ATCC 4973 16S ribosomal RNA gene, partial sequence	AF094733	1458	ENA	296 tax ID	*
[Ref.: #20218]	Pseudomonas fragi strain ATCC 4973 16S ribosomal RNA gene, partial sequence	FJ971871	791	ENA	296 tax ID	*
[Ref.: #20218]	Pseudomonas fragi 16S-23S intergenic spacer strain CECT 446	AJ279241	324	ENA	296 tax ID	*
[Ref.: #20218]	Pseudomonas fragi gene for 16S rRNA, strain: IFO 3458	AB021413	1527	ENA	296 tax ID	*
[Ref.: #20218]	Pseudomonas fragi gene for 16S rRNA, partial sequence, strain: NBRC 3458	AB680088	1462	ENA	296 tax ID	*

Availability in culture collections External links

[Ref.: #1314]

Culture collection no.

DSM 3456, ATCC 4973, NRRL B-25, WDCM 00116

[Ref.: #20218]

Associated Passport(s) in StrainInfo

53192, 53183, 53198, 866243

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Genetics	Structure of the glycocalyx polysaccharide of Pseudomonas fragi ATCC 4973.	Parolis LA, Parolis H, Dutton GG, Wing PL, Skura BJ	Carbohydr Res	10.1016/0008-6215(92)84184-t	1991	*
Cultivation	Centroid search optimization of cultural conditions affecting the production of extracellular proteinase by Pseudomonas fragi ATCC 4973.	Myhara RM, Skura B	J Appl Bacteriol	10.1111/j.1365-2672.1990.tb01545.x	1990	*
Enzymology	Ultrastructural localization of an extracellular protease in Pseudomonas fragi by using the peroxidase-antiperoxidase reaction.	Thompson SS, Naidu YM, Pestka JJ	Appl Environ Microbiol	10.1128/aem.50.4.1038-1042.1985	1985	*
Phylogeny	Numerical taxonomy of psychrotrophic pseudomonads.	Molin G, Ternstrom A	J Gen Microbiol	10.1099/00221287-128-6-1249	1982	*
Metabolism	Acquisition of iron by the non-siderophore-producing Pseudomonas fragi.	Champomier-Verges MC, Stintzi A, Meyer JM	Microbiology (Reading)	10.1099/13500872-142-5-1191	1996	*
Biotechnology	The differential adherence capabilities of two Listeria monocytogenes strains in monoculture and multispecies biofilms as a function of temperature.	Norwood DE, Gilmour A	Lett Appl Microbiol	10.1046/j.1472-765x.2001.01004.x	2001	*
Metabolism	Control of pyrimidine synthesis in Pseudomonas fragi.	West TP	Lett Appl Microbiol	10.1046/j.1472-765x.2002.01203.x	2002	*
Biotechnology	Production of volatile compounds in cheese by Pseudomonas fragi strains of dairy origin.	Morales P, Fernandez-Garcia E, Nunez M	J Food Prot	10.4315/0362-028x-68.7.1399	2005	*
Transcriptome	Cold stress promoting a psychrotolerant bacterium Pseudomonas fragi P121 producing trehaloase.	Mei YZ, Huang PW, Liu Y, He W, Fang WW	World J Microbiol Biotechnol	10.1007/s11274-016-2097-1	2016	*
Phylogeny	Pseudomonas bubulae sp. nov., isolated from beef.	Lick S, Krockel L, Wibberg D, Winkler A, Blom J, Goesmann A, Kalinowski J	Int J Syst Evol Microbiol	10.1099/ijsem.0.003751	2020	*
Phylogeny	Pseudomonas saxonica sp. nov., isolated from raw milk and skimmed milk concentrate.	Hofmann K, Huptas C, Doll EV, Scherer S, Wenning M	Int J Syst Evol Microbiol	10.1099/ijsem.0.003851	2020	*
Phylogeny	Pseudomonas paraversuta sp. nov. isolated from refrigerated dry-aged beef.	Lick S, Wibberg D, Winkler A, Blom J, Grimmler C, Goesmann A, Kalinowski J, Krockel L	Int J Syst Evol Microbiol	10.1099/ijsem.0.004822	2021	*
Enzymology	Phenolic Profile, Antioxidant Capacity and Antimicrobial Activity of Nettle Leaves Extracts Obtained by Advanced Extraction Techniques.	Elez Garofulic I, Malin V, Repajic M, Zoric Z, Pedisic S, Sternisa M, Smole Mozina S, Dragovic-Uzelac V	Molecules	10.3390/molecules26206153	2021	*
	Scanning electron microscope study of Pseudomonas fragi on intact and sarcoplasm-depleted bovine longissimus dorsi muscle.	Yada RY, Skura BJ	Appl Environ Microbiol	10.1128/aem.43.4.905-915.1982	1982	*
	Functional Characteristics of Extracellular Vesicles Produced by Pseudomonas fragi ATCC 4973.	Myhara RM, Skura BJ, Patel T, Nakajima M	J Food Prot	10.4315/0362-028X-53.10.859	1990	*
	Biofilm Formation by Listeria monocytogenes Utilizes a Primary Colonizing Microorganism in Flowing Systems.	Sasahara KC, Zottola EA	J Food Prot	10.4315/0362-028X-56.12.1022	1993	*
	Efficacy of olive leaf extract (Olea europaea L. cv Gentile di Larino) in marinated anchovies (Engraulis encrasicolus, L.) process.	Testa B, Lombardi SJ, Macciola E, Succi M, Tremonte P, Iorizzo M	Heliyon	10.1016/j.heliyon.2019.e01727	2019	*

References

#1314

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3456

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36579 ; Curators of the CIP;

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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