Pseudomonas citronellolis (DSM 50332, ATCC 13674, CIP 104381)

Name: Pseudomonas citronellolis (DSM 50332, ATCC 13674, CIP 104381)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12823

Type strain

Culture col. no. DSM 50332 ATCC 13674 CIP 104381 CCUG 17933 LMG 18378 10 more

NCBI tax ID(s) 1215097 53408

Special Collections DSMZ CCUG JCM KCTC CIP

History <- ATCC <- W. Seubert K. Komagata <-- R. Hugh 846 <-- ATCC 13674 <-- W. Seubert. CIP <- 1995, DSM <- ATCC <- W. Seubert

Links

Pseudomonas citronellolis DSM 50332 is an aerobe, Gram-negative, rod-shaped bacterium that was isolated from soil under pine trees.

Gram-negative rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 11919

LMG 18378,ATCC 13674,CIP 104381,DSM 50332,TBRC:8616,NCIMB 12783,CCUG 17933,BCRC 14345,CCRC 14345,CCRC 13907,NRRL B-2504,CFBP 5585,KCTC 12148,KACC 10776,IAM 15129,JCM 21587,NBRC 103043

@ref 20215

Last LPSN update 2026-02-09 11:20:57

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Pseudomonas

Species Pseudomonas citronellolis

Full scientific name Pseudomonas citronellolis Seubert 1960 (Approved Lists 1980)

Synonyms (1)

Pseudomonas humi

BacDive ID	Other strains from **Pseudomonas citronellolis** (12)	Type strain
12819	P. citronellolis ADP, DSM 11735, CCUG 54931
12820	P. citronellolis D21, DSM 13132
12821	P. citronellolis D 26, DSM 13957
12822	P. citronellolis 6903, BG 6903, DSM 18650, CCUG 54947
151000	P. citronellolis CCUG 42982
154310	P. citronellolis CCUG 53022
154319	P. citronellolis CCUG 53068
155705	P. citronellolis CCUG 59066
156712	P. citronellolis CCUG 64306 A
156756	P. citronellolis CCUG 64909
157220	P. citronellolis CCUG 70659
164553	P. citronellolis JCM 33429

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
118305	negative	rod-shaped
125438	negative		98.485
125439			92.5
125439	negative		97.7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
12711	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
40147	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
118305	CIP Medium 3	Medium recipe at CIP
118305	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
12711	positive	growth	30
40147	positive	growth	30
46720	positive	growth	30
67770	positive	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
46720	aerobe
118305	obligate aerobe
125438	aerobe	91.119

Spore formation

@ref	Spore formation	Confidence
125439		97.3

Compound production

@ref	Compound
12711	polyhydroxyalkanoic acids
12711	isoprene

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	+	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
118305	17632 ChEBI	nitrate	+	reduction
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
118305	16301 ChEBI	nitrite	+	reduction
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
118305	35581 ChEBI	indole
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	+	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
118305	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68369	gelatinase	-		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
118305	oxidase	+
68382	trypsin	+	3.4.21.4	from API zym
118305	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
118305	-	+	+	+	-	+	-	-	+	-	+	+	-	-	-	+	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
12711	+	-	-	+	-	-	-	-	+	-	-	-	-	-	+	+	-	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Tree

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
12711	soil under pine trees	Virginia	USA	USA	North America
46720	Soil under pine trees		USA	USA	North America
67770	Soil under pine trees in northern Virginia		USA	USA	North America
118305	Environment, Soil under pine trees

Taxonmaps

69479

Global distribution of 16S sequence Z76659 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	31
Soil Samples	16
Animal Samples	53
Plant Samples	12
Total Samples	112

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
12711	1	Risk group (German classification) According to TRBA 466
118305	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM474545v1 assembly for Pseudomonas citronellolis DSM 50332	contig	GCA_004745455	53408.24	8118036653	53408	68.44
67770	IMG-taxon 2663762793 annotated assembly for Pseudomonas citronellolis LMG 18378	scaffold	GCA_900112375	53408.9	2663762793	53408	56.68
67770	ASM209155v1 assembly for Pseudomonas citronellolis NBRC 103043	contig	GCA_002091555	1215097.3	2969343799	1215097	54.1

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Pseudomonas citronellolis gene for 16S rRNA, strain: ATCC 13674	AB021396	1499	53408
12711	P.citronellolis 16S rRNA gene	Z76659	1510	53408
67770	Pseudomonas citronellolis gene for 16S rRNA, partial sequence, strain: NBRC 103043	AB681923	1462	53408
124043	Pseudomonas citronellolis strain NBRC 103043 16S ribosomal RNA gene, partial sequence.	MN726430	325	53408

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Pseudomonas citronellolis LMG 18378
NCBI: GCA_900112375

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	69.80	no
125439	gram_stain	BacteriaNetⓘ	negative	97.70	no
125439	motility	BacteriaNetⓘ	yes	92.50	no
125439	spore_formation	BacteriaNetⓘ	no	97.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudomonas citronellolis NBRC 103043 strain NBRC 103043
PATRIC: 1215097.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.49	no
125438	anaerobic	anaerobicⓘ	no	97.57	yes
125438	spore-forming	spore-formingⓘ	no	84.05	no
125438	aerobic	aerobicⓘ	yes	91.12	yes
125438	thermophilic	thermophileⓘ	no	99.50	yes
125438	flagellated	motile2+ⓘ	yes	88.75	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	First report of infection with Pseudomonas citronellolis: a case of urosepsis.	Williams G.	New Microbes New Infect	10.1016/j.nmni.2019.100531	2019
Biotechnology	Utilization of food waste streams for the production of biopolymers.	Ranganathan S, Dutta S, Moses JA, Anandharamakrishnan C.	Heliyon	10.1016/j.heliyon.2020.e04891	2020
	Critical Review on the Progress of Plastic Bioupcycling Technology as a Potential Solution for Sustainable Plastic Waste Management.	Lomwongsopon P, Varrone C.	Polymers (Basel)	10.3390/polym14224996	2022
Metabolism	Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: purification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13.	Schirmer A, Jendrossek D, Schlegel HG.	Appl Environ Microbiol	10.1128/aem.59.4.1220-1227.1993	1993
	Microbial monoterpene transformations-a review.	Marmulla R, Harder J.	Front Microbiol	10.3389/fmicb.2014.00346	2014
	Mortality causes universal changes in microbial community composition.	Abreu CI, Friedman J, Andersen Woltz VL, Gore J.	Nat Commun	10.1038/s41467-019-09925-0	2019
	Metabolite-mediated modelling of microbial community dynamics captures emergent behaviour more effectively than species-species modelling.	Brunner JD, Chia N.	J R Soc Interface	10.1098/rsif.2019.0423	2019
	Interspecies bacterial competition regulates community assembly in the C. elegans intestine.	Ortiz A, Vega NM, Ratzke C, Gore J.	ISME J	10.1038/s41396-021-00910-4	2021
Metabolism	Autotransporter domain-dependent enzymatic analysis of a novel extremely thermostable carboxylesterase with high biodegradability towards pyrethroid pesticides.	Cai X, Wang W, Lin L, He D, Huang G, Shen Y, Wei W, Wei D.	Sci Rep	10.1038/s41598-017-03561-8	2017
Metabolism	Biodegradation of acyclic isoprenoids by Pseudomonas species.	Cantwell SG, Lau EP, Watt DS, Fall RR.	J Bacteriol	10.1128/jb.135.2.324-333.1978	1978
	Isolation and Characterization of a Pseudomonas sp. That Mineralizes the s-Triazine Herbicide Atrazine.	Mandelbaum RT, Allan DL, Wackett LP.	Appl Environ Microbiol	10.1128/aem.61.4.1451-1457.1995	1995
Biotechnology	Biotechnological Production, Isolation and Characterisation of (2R,3S)-2,3-Dihydroxy-2,3-Dihydrobenzoate.	Kiel M, Barrantes I, Pieper DH, Engesser KH.	Microb Biotechnol	10.1111/1751-7915.70228	2025
Genetics	Comparative Genomic Analysis of Three Pseudomonas Species Isolated from the Eastern Oyster (Crassostrea virginica) Tissues, Mantle Fluid, and the Overlying Estuarine Water Column.	Pathak A, Stothard P, Chauhan A.	Microorganisms	10.3390/microorganisms9030490	2021
	Polyhydroxyalkanoate Production from Eucalyptus Bark's Enzymatic Hydrolysate.	Rodrigues T, Torres CAV, Marques S, Girio F, Freitas F, Reis MAM.	Materials (Basel)	10.3390/ma17081773	2024
Biotechnology	Manipulating Microbial Cell Morphology for the Sustainable Production of Biopolymers.	Kalia VC, Patel SKS, Karthikeyan KK, Jeya M, Kim IW, Lee JK.	Polymers (Basel)	10.3390/polym16030410	2024
	Bacterial species-structure-property relationships of polyhydroxyalkanoate biopolymers produced on simple sugars for thin film applications.	Attenborough E, Yazdan Parast F, Nosrati R, Banaszak Holl MM, van 't Hag L.	Microb Cell Fact	10.1186/s12934-025-02833-7	2025
	Recent updates to microbial production and recovery of polyhydroxyalkanoates.	de Melo RN, de Souza Hassemer G, Steffens J, Junges A, Valduga E.	3 Biotech	10.1007/s13205-023-03633-9	2023
Phylogeny	Producing and Characterizing Polyhydroxyalkanoates from Starch and Chickpea Waste Using Mixed Microbial Cultures in Solid-State Fermentation.	Grgurevic K, Bramberger D, Miloloza M, Stublic K, Ocelic Bulatovic V, Ranilovic J, Ukic S, Bolanca T, Cvetnic M, Markic M, Kucic Grgic D.	Polymers (Basel)	10.3390/polym16233407	2024
	Optimization of a Two-Species Microbial Consortium for Improved Mcl-PHA Production From Glucose-Xylose Mixtures.	Zhu Y, Ai M, Jia X.	Front Bioeng Biotechnol	10.3389/fbioe.2021.794331	2021
	Apple orchard waste recycling and valorization of valuable product-A review.	Duan Y, Mehariya S, Kumar A, Singh E, Yang J, Kumar S, Li H, Kumar Awasthi M.	Bioengineered	10.1080/21655979.2021.1872905	2021
	The Use of Phage Cocktail and Various Antibacterial Agents in Combination to Prevent the Emergence of Phage Resistance.	Duc HM, Zhang Y, Hoang SM, Masuda Y, Honjoh KI, Miyamoto T.	Antibiotics (Basel)	10.3390/antibiotics12061077	2023
	Artificial symbiont replacement in a vertically transmitted plant symbiosis reveals a role for microbe-microbe interactions in enforcing specificity.	Ninzatti L, Sana TG, Acar T, Moreau S, Jardinaud MF, Marti G, Coen O, Carlier AL.	ISME J	10.1093/ismejo/wraf177	2025
	Synergistic and additive interactions of Shewanella sp., Pseudomonas sp. and Thauera sp. with chlorantraniliprole and emamectin benzoate for controlling Spodoptera litura (Fabricius).	Sarkhandia S, Sharma G, Mahajan R, Koundal S, Kumar M, Chadha P, Saini HS, Kaur S.	Sci Rep	10.1038/s41598-023-41641-0	2023
	Culturing-Enriched Metabarcoding Analysis of the Oryctes rhinoceros Gut Microbiome.	Shelomi M, Chen MJ.	Insects	10.3390/insects11110782	2020
	Preparation and Characterization of Films Based on a Natural P(3HB)/mcl-PHA Blend Obtained through the Co-culture of Cupriavidus Necator and Pseudomonas Citronellolis in Apple Pulp Waste.	Rebocho AT, Pereira JR, Neves LA, Alves VD, Sevrin C, Grandfils C, Freitas F, Reis MAM	Bioengineering (Basel)	10.3390/bioengineering7020034	2020
Metabolism	Potential for mcl-PHA production from nonanoic and azelaic acids.	Gillis J, Ko K, Ramsay JA, Ramsay BA	Can J Microbiol	10.1139/cjm-2017-0554	2017
Metabolism	Pyrimidine nucleotide synthesis in Pseudomonas citronellolis.	West TP	Can J Microbiol	10.1139/w04-028	2004
Enzymology	Molecular cloning of the carboxylesterase gene and biochemical characterization of the encoded protein from Pseudomonas citronellolis ATCC 13674.	Chao YP, Fu H, Wang YL, Huang WB, Wang JY	Res Microbiol	10.1016/S0923-2508(03)00144-X	2003
Metabolism	Intracellular degradation of two structurally different polyhydroxyalkanoic acids accumulated in Pseudomonas putida and Pseudomonas citronellolis from mixtures of octanoic acid and 5-phenylvaleric acid.	Chung DM, Choi MH, Song JJ, Yoon SC, Kang IK, Huh NE	Int J Biol Macromol	10.1016/s0141-8130(01)00172-6	2001
Metabolism	Microbial degradation of monoterpenes in the absence of molecular oxygen.	Harder J, Probian C	Appl Environ Microbiol	10.1128/aem.61.11.3804-3808.1995	1995
	Polyester Biosynthesis Characteristics of Pseudomonas citronellolis Grown on Various Carbon Sources, Including 3-Methyl-Branched Substrates.	Choi MH, Yoon SC	Appl Environ Microbiol	10.1128/aem.60.9.3245-3254.1994	1994
Phylogeny	Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site.	Gupta SK, Kumari R, Prakash O, Lal R	Int J Syst Evol Microbiol	10.1099/ijs.0.65401-0	2008
Phylogeny	Pseudomonas knackmussii sp. nov.	Stolz A, Busse HJ, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.64761-0	2007
Phylogeny	Pseudomonas delhiensis sp. nov., from a fly ash dumping site of a thermal power plant.	Prakash O, Kumari K, Lal R	Int J Syst Evol Microbiol	10.1099/ijs.0.64456-0	2007
Phylogeny	Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea.	Kwon SW, Kim JS, Park IC, Yoon SH, Park DH, Lim CK, Go SJ	Int J Syst Evol Microbiol	10.1099/ijs.0.02326-0	2003
Phylogeny	Pseudomonas humi sp. nov., isolated from leaf soil.	Akita H, Kimura ZI, Hoshino T	Arch Microbiol	10.1007/s00203-018-1588-x	2018

References

#12711	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 50332
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#40147	; Curators of the CIP;
#46720	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 17933
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118305	Collection of Institut Pasteur ; Curators of the CIP; CIP 104381
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudomonas citronellolis (DSM 50332, ATCC 13674, CIP 104381)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_004745455

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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