Azotobacter nigricans (DSM 2288, ATCC 35009, UQM 1967)

Name: Azotobacter nigricans (DSM 2288, ATCC 35009, UQM 1967)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12755

Type strain

Strain Designation WR-128

Culture col. no. DSM 2288 ATCC 35009 UQM 1967 WR-128

NCBI tax ID(s) 270344 270492

Special Collections DSMZ

History <- WR <- E. Afrikian <- N. A. Krassilnikov

Links

Azotobacter nigricans WR-128 is a bacterium of the family Pseudomonadaceae.

16S sequence Bacteria

Name and taxonomic classification

SI-ID 1079

LMG 2816,DSM 2288,TBRC 7412,ATCC 35009,ACM 1967,IAM 15005

@ref 20215

Last LPSN update 2026-02-09 11:20:56

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Azotobacter

Species Azotobacter nigricans

Full scientific name Azotobacter nigricans Krassilnikov 1949 (Approved Lists 1980)

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1020	DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441)		Medium recipe at MediaDive	Name: DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441) Composition: Agar 14.881 g/l DL-Malate 1.98413 g/l D-Glucose 1.98413 g/l D-Mannitol 1.98413 g/l Na-pyruvate 0.992063 g/l Disodium succinate 0.992063 g/l K2HPO4 0.892857 g/l KH2PO4 0.0992065 g/l NaCl 0.0992065 g/l CaCl2 x 2 H2O 0.0992065 g/l MgSO4 x 7 H2O 0.0992065 g/l Yeast extract 0.0496032 g/l FeSO4 x 7 H2O 0.00992065 g/l MnSO4 x H2O 0.00496032 g/l NaVO3 x H2O 0.00496032 g/l Nicotinic acid 0.00248016 g/l Pyridoxine hydrochloride 0.00248016 g/l Calcium pantothenate 0.00248016 g/l Thiamine-HCl x 2 H2O 0.00248016 g/l H3BO3 0.000892857 g/l CoCl2 x 6 H2O 0.000595238 g/l Riboflavin 0.000496032 g/l ZnSO4 x 7 H2O 0.000297619 g/l MnCl2 x 4 H2O 8.92857e-05 g/l Na2MoO4 x 2 H2O 8.92857e-05 g/l NiCl2 x 6 H2O 5.95238e-05 g/l Vitamin B12 4.96032e-05 g/l CuCl2 x 2 H2O 2.97619e-05 g/l Folic acid 9.92063e-06 g/l Biotin 4.96032e-06 g/l Distilled water
1020	AZOTOBACTER MEDIUM (DSMZ Medium 3)		Medium recipe at MediaDive	Name: AZOTOBACTER MEDIUM (DSMZ Medium 3) Composition: Agar 15.0 g/l CaCO3 5.0 g/l Glucose 5.0 g/l Mannitol 5.0 g/l K2HPO4 0.9 g/l KH2PO4 0.1 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 2 H2O 0.1 g/l FeSO4 x 7 H2O 0.01 g/l Na2MoO4 x 2 H2O 0.005 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
1020	positive	growth	30
1020	positive	growth	28

Physiology and metabolism

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1020	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
1020	Azotobacter nigricans subsp. nigricans gene for 16S rRNA, partial sequence	AB175651	1475		270492

GC content

1020

GC-content (mol%)64.5

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Enhancing Phosphorus and Nitrogen Uptake in Maize Crops with Food Industry Biosolids and Azotobacter nigricans.	Vera-Garcia SL, Rodriguez-Casasola FN, Barrera-Cortes J, Albores-Medina A, Munoz-Paez KM, Canizares-Villanueva RO, Montes-Horcasitas MC.	Plants (Basel)	10.3390/plants12173052	2023
Enzymology	Synergistic Effect of Azotobacter nigricans and Nitrogen Phosphorus Potassium Fertilizer on Agronomic and Yieldtraits of Maize (Zea mays L.).	Sagar A, Sayyed RZ, Ramteke PW, Ramakrishna W, Poczai P, Al Obaid S, Ansari MJ.	Front Plant Sci	10.3389/fpls.2022.952212	2022
	Investigating the role of bentonite clay with different soil amendments to minimize the bioaccumulation of heavy metals in Solanum melongena L. under the irrigation of tannery wastewater.	Khan WUD, Wei X, Ali HH, Zulfiqar F, Chen J, Iqbal R, Zaheer MS, Ali B, Ghafoor S, Rabiya UE, Waqas M, Ghaffar R, Soufan W, El Sabagh A.	Front Plant Sci	10.3389/fpls.2022.958978	2022
	Biocontrol of Fusarium equiseti using chitosan nanoparticles combined with Trichoderma longibrachiatum and Penicillium polonicum.	El-Morsy EM, Elmalahy YS, Mousa MMA.	Fungal Biol Biotechnol	10.1186/s40694-023-00151-4	2023
	Statistical modeling-approach for optimization of Cu²⁺ biosorption by Azotobacter nigricans NEWG-1; characterization and application of immobilized cells for metal removal.	Ghoniem AA, El-Naggar NE, Saber WIA, El-Hersh MS, El-Khateeb AY.	Sci Rep	10.1038/s41598-020-66101-x	2020
	Comparative Evaluation of Bruker Biotyper and ASTA MicroIDSys for Species Identification in a Clinical Microbiology Laboratory.	Chung Y, Han M, Kim JS.	Diagnostics (Basel)	10.3390/diagnostics11091683	2021
	Antifungal activity of Azotobacter nigricans against trichothecene-producing Fusarium species associated with cereals.	Nagaraja H, Chennappa G, Rakesh S, Naik MK, Amaresh YS, Sreenivasa MY.	Food Sci Biotechnol	10.1007/s10068-016-0190-8	2016
Phylogeny	Pesticide tolerant Azotobacter isolates from paddy growing areas of northern Karnataka, India.	Chennappa G, Adkar-Purushothama CR, Suraj U, Tamilvendan K, Sreenivasa MY.	World J Microbiol Biotechnol	10.1007/s11274-013-1412-3	2014
Metabolism	Encystment of Azotobacter nigricans grown diazotrophically on kerosene as sole carbon source.	Garcia-Esquivel G, Calva-Calva G, Ferrera-Cerrato R, Fernandez-Linares LC, Vazquez RR, Esparza-Garcia FJ.	Arch Microbiol	10.1007/s00203-008-0444-9	2009
	Rotatable central composite design versus artificial neural network for modeling biosorption of Cr⁶⁺ by the immobilized Pseudomonas alcaliphila NEWG-2.	Saber WIA, El-Naggar NE, El-Hersh MS, El-Khateeb AY, Elsayed A, Eldadamony NM, Ghoniem AA.	Sci Rep	10.1038/s41598-021-81348-8	2021
	[Formation of artificial nitrogen-fixing symbiosis with rape (Brassica napus var. napus) plants in nonsterile soil].	Koval'skaia NIu, Lobakova ES, Umarov MM.	Mikrobiologiia	10.1023/a:1012324609202	2001
Enzymology	Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria.	Fallik E, Chan YK, Robson RL	J Bacteriol	10.1128/jb.173.1.365-371.1991	1991

References

#1020	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2288
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Azotobacter nigricans (DSM 2288, ATCC 35009, UQM 1967)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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