Pseudoalteromonas tunicata (DSM 14096, CCUG 26757, D2)

Name: Pseudoalteromonas tunicata (DSM 14096, CCUG 26757, D2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12688

Type strain

Strain Designation D2

Culture col. no. DSM 14096 CCUG 26757 D2 CIP 105928 CCUG 44952

NCBI tax ID(s) 314281

Special Collections DSMZ CCUG CIP

History <- C. Holmström; D2 CIP <- 1999, CCUG <- C. Holmström, Univ. Göteborg, Sweden: strain D2

Links

Pseudoalteromonas tunicata D2 is a psychrophilic, Gram-negative, motile prokaryote that was isolated from adult tunicate Ciona intestinalis.

Gram-negative motile rod-shaped psychrophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 58170

CCUG 26757,DSM 14096,CIP 105928,CCUG44952,CCUG 44952 A

@ref 20215

Last LPSN update 2025-12-16 09:49:54

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Alteromonadales

Family Pseudoalteromonadaceae

Genus Pseudoalteromonas

Species Pseudoalteromonas tunicata

Full scientific name Pseudoalteromonas tunicata Holmström et al. 1998

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
123689	negative	rod-shaped
125439	negative		99
125438			90.046
125438	negative		100

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5147	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)	Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
42038	MEDIUM 480 - for Pseudomonas tunicata		Sodium hydrogen carbonate (0.080 g);Distilled water make up to (1000.000 ml);Sodium chloride (17.600 g);Potassium chloride (0.250 g);Iron (II) sulphate heptahydrate(0.010 g);Boric acid (0.008 g);Magnesium chloride hexahydrate (1.870 g);Calcium chloridedih
123689	CIP Medium 480	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
5147	positive	growth	20	psychrophilic
42038	positive	growth	20	psychrophilic
123689	positive	growth	5-30
123689	negative	growth	37
123689	negative	growth	41

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	98.7

Spore formation

@ref	Spore formation	Confidence
125439		98.9

Halophily

@ref	Salt	Growth	Tested relation	Concentration
123689	NaCl	positive	growth	2-4 %
123689	NaCl		growth	0 %
123689	NaCl		growth	6 %
123689	NaCl		growth	8 %
123689	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
123689	4853 ChEBI	esculin	-	hydrolysis
123689	17632 ChEBI	nitrate	-	reduction
123689	16301 ChEBI	nitrite	-	reduction

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
123689	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123689	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123689	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123689	amylase	+
68382	beta-galactosidase	-	3.2.1.23	from API zym
123689	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123689	caseinase	-	3.4.21.50
123689	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
123689	DNase	+
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
123689	gelatinase	+
123689	lecithinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
123689	lipase	-
68382	lipase (C 14)	-		from API zym
123689	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123689	ornithine decarboxylase	-	4.1.1.17
123689	oxidase	-
123689	protease	-
68382	trypsin	-	3.4.21.4	from API zym
123689	tryptophan deaminase	-
123689	tween esterase	+
123689	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123689	-	+	-	+	-	+	-	-	-	-	+	+	-	-	-	-	-	+	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
123689	not determinedn.d.	-	-	-	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Invertebrates (Other)	#Tunicata

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
5147	adult tunicate Ciona intestinalis	Ciona intestinalis	western coast	Sweden	SWE	Europe
123689	Environment, Water surface, adult tunicate, Ciona intestinalis		Göteborg	Sweden	SWE	Europe	1990

Taxonmaps

69479

Global distribution of 16S sequence Z25522 (>99% sequence identity) for Pseudoalteromonas from Microbeatlas

Aquatic Samples	15
Soil Samples
Animal Samples	16
Plant Samples
Total Samples	31

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5147	1	Risk group (German classification) According to TRBA 466
123689	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM231081v1 assembly for Pseudoalteromonas tunicata D2	complete	GCA_002310815	314281.3		314281	99.11
66792	ASM356882v1 assembly for Pseudoalteromonas tunicata D2	complete	GCA_003568825	314281.6	2847754586	314281	98.91
66792	ASM15324v1 assembly for Pseudoalteromonas tunicata D2	scaffold	GCA_000153245	87626.5	638341157	87626	49.48

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5147	Pseudoalteromonas tunicata 16S RNA gene, partial, strain D2	Z25522	1365		87626

GC content

5147

GC-content (mol%)42

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Pseudoalteromonas tunicata D2
NCBI: GCA_003568825

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	98.90	no
125439	motility	BacteriaNetⓘ	yes	68.30	no
125439	gram_stain	BacteriaNetⓘ	negative	99.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudoalteromonas tunicata D2
NCBI: GCA_003568825.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	100.00	no
125438	anaerobic	anaerobicⓘ	no	91.47	no
125438	aerobic	aerobicⓘ	yes	77.50	no
125438	spore-forming	spore-formingⓘ	no	85.46	no
125438	thermophilic	thermophileⓘ	no	98.78	yes
125438	flagellated	motile2+ⓘ	yes	90.05	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Crown ether dopant to reduce ion suppression and improve detection in the liquid microjunction surface sampling probe.	Metwally H, Yu J, Theriault R, Kolwich JL, Ellis R, Ross AC, Oleschuk RD.	Rapid Commun Mass Spectrom	10.1002/rcm.9889	2024
Pathogenicity	Dual function of tropodithietic acid as antibiotic and signaling molecule in global gene regulation of the probiotic bacterium Phaeobacter inhibens.	Beyersmann PG, Tomasch J, Son K, Stocker R, Goker M, Wagner-Dobler I, Simon M, Brinkhoff T.	Sci Rep	10.1038/s41598-017-00784-7	2017
Metabolism	Tropodithietic acid production in Phaeobacter gallaeciensis is regulated by N-acyl homoserine lactone-mediated quorum sensing.	Berger M, Neumann A, Schulz S, Simon M, Brinkhoff T.	J Bacteriol	10.1128/jb.05818-11	2011
	Exopolymeric Substances Control Microbial Community Structure and Function by Contributing to both C and Fe Nutrition in Fe-Limited Southern Ocean Provinces.	Blanco-Ameijeiras S, Cabanes DJE, Cable RN, Trimborn S, Jacquet S, Wiegmann S, Volkner C, Lelchat F, Bracher A, Duhaime MB, Hassler CS.	Microorganisms	10.3390/microorganisms8121980	2020
Metabolism	Genetic analysis of the upper phenylacetate catabolic pathway in the production of tropodithietic acid by Phaeobacter gallaeciensis.	Berger M, Brock NL, Liesegang H, Dogs M, Preuth I, Simon M, Dickschat JS, Brinkhoff T.	Appl Environ Microbiol	10.1128/aem.07657-11	2012
	ChrII-encoded DNA helicase: A preliminary study.	Tang B, Chen Z, Xia H, Wang R, Song X	Protein Pept Lett	10.2174/0929866530666221104112210	2022
Phylogeny	Phaeobacter porticola sp. nov., an antibiotic-producing bacterium isolated from a sea harbour.	Breider S, Freese HM, Sproer C, Simon M, Overmann J, Brinkhoff T	Int J Syst Evol Microbiol	10.1099/ijsem.0.001879	2017
Phylogeny	Pseudoalteromonas tunicata sp. nov., a bacterium that produces antifouling agents.	Holmstrom C, James S, Neilan BA, White DC, Kjelleberg S	Int J Syst Bacteriol	10.1099/00207713-48-4-1205	1998

References

#5147	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14096
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#42038	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123689	Collection of Institut Pasteur ; Curators of the CIP; CIP 105928
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudoalteromonas tunicata (DSM 14096, CCUG 26757, D2)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002310815

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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