Naumannella halotolerans (DSM 24323, LMG 26184, WS 4616)

Name: Naumannella halotolerans (DSM 24323, LMG 26184, WS 4616)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12674

Type strain

Strain Designation WS 4616

Culture col. no. DSM 24323 LMG 26184 WS 4616 JCM 30997

NCBI tax ID(s) 993414

Special Collections DSMZ JCM Literature strains

History DSMZ <- S. Scherer, Research Center for Nutrition and Food Sci. (ZIEL), TU Munich, Germany; WS4616 <- G. Rieser JCM <-- LMG 26184 <-- S. Scherer; Tech. Univ. of Munich, Germany <-- G. Rieser; Tech. Univ. of Munich, Germany; WS4616.

Links

Naumannella halotolerans WS 4616 is an aerobe, Gram-positive, coccus-shaped bacterium that was isolated from pharmaceutical clean room.

Gram-positive coccus-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 374254

LMG 26184,DSM 24323,JCM 30997

@ref 20215

Last LPSN update 2026-05-29 08:27:51

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Propionibacteriales

Family Propionibacteriaceae

Genus Naumannella

Species Naumannella halotolerans

Full scientific name Naumannella halotolerans Rieser et al. 2012

BacDive ID	Other strains from **Naumannella halotolerans** (1)	Type strain
156433	N. halotolerans CCUG 61721

Morphology

Cell morphology

@ref	Gram stain	Cell width	Cell shape	Confidence
30487	positive	1.15 µm	coccus-shaped
125438				96.488
125438	positive			92.506

Pigmentation

30487

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
17435	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)		Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
17435	positive	growth	28
30487	positive	growth	15-37
67770	positive	growth	28

Culture pH

@ref	Ability	Type	PH
30487	positive	growth	06-08

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
30487	aerobe
125438	aerobe	90.6
125439	obligate aerobe	98.936

Spore formation

30487

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
30487	NaCl	positive	growth	0-10 %

Observation

67770

Observationquinones: MK-9(H₄), MK-8(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
30487	22599 ChEBI	arabinose	+	carbon source
30487	4853 ChEBI	esculin	+	hydrolysis
30487	28757 ChEBI	fructose	+	carbon source
30487	28260 ChEBI	galactose	+	carbon source
30487	17234 ChEBI	glucose	+	carbon source
30487	17754 ChEBI	glycerol	+	carbon source
30487	18403 ChEBI	L-arabitol	+	carbon source
30487	17306 ChEBI	maltose	+	carbon source
30487	29864 ChEBI	mannitol	+	carbon source
30487	37684 ChEBI	mannose	+	carbon source
30487	15963 ChEBI	ribitol	+	carbon source
30487	33942 ChEBI	ribose	+	carbon source
30487	30911 ChEBI	sorbitol	+	carbon source
30487	17992 ChEBI	sucrose	+	carbon source
30487	27082 ChEBI	trehalose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
30487	catalase	+	1.11.1.6
30487	cytochrome oxidase	+	1.9.3.1
30487	urease	+	3.5.1.5

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	adipate degradation	100	2 of 2
66794	L-lactaldehyde degradation	100	3 of 3
66794	enterobactin biosynthesis	100	3 of 3
66794	acetate fermentation	100	4 of 4
66794	palmitate biosynthesis	100	22 of 22
66794	ppGpp biosynthesis	100	4 of 4
66794	methylglyoxal degradation	100	5 of 5
66794	valine metabolism	100	9 of 9
66794	lipoate biosynthesis	100	5 of 5
66794	phenylmercury acetate degradation	100	2 of 2
66794	starch degradation	100	10 of 10
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	taurine degradation	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	vitamin K metabolism	100	5 of 5
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	proline metabolism	90.91	10 of 11
66794	threonine metabolism	90	9 of 10
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	gluconeogenesis	87.5	7 of 8
66794	peptidoglycan biosynthesis	86.67	13 of 15
66794	pyrimidine metabolism	86.67	39 of 45
66794	photosynthesis	85.71	12 of 14
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	phenylalanine metabolism	84.62	11 of 13
66794	NAD metabolism	83.33	15 of 18
66794	metabolism of disaccharids	81.82	9 of 11
66794	pentose phosphate pathway	81.82	9 of 11
66794	purine metabolism	80.85	76 of 94
66794	Entner Doudoroff pathway	80	8 of 10
66794	flavin biosynthesis	80	12 of 15
66794	factor 420 biosynthesis	80	4 of 5
66794	heme metabolism	78.57	11 of 14
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	serine metabolism	77.78	7 of 9
66794	allantoin degradation	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	sulfopterin metabolism	75	3 of 4
66794	glutamate and glutamine metabolism	75	21 of 28
66794	butanoate fermentation	75	3 of 4
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	cyclohexanol degradation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	non-pathway related	73.68	28 of 38
66794	vitamin B6 metabolism	72.73	8 of 11
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	citric acid cycle	71.43	10 of 14
66794	glycolysis	70.59	12 of 17
66794	oxidative phosphorylation	70.33	64 of 91
66794	propionate fermentation	70	7 of 10
66794	leucine metabolism	69.23	9 of 13
66794	methionine metabolism	69.23	18 of 26
66794	histidine metabolism	68.97	20 of 29
66794	alanine metabolism	68.97	20 of 29
66794	methane metabolism	66.67	2 of 3
66794	arginine metabolism	66.67	16 of 24
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	acetoin degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	lipid metabolism	64.52	20 of 31
66794	carotenoid biosynthesis	63.64	14 of 22
66794	degradation of sugar alcohols	62.5	10 of 16
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	isoprenoid biosynthesis	61.54	16 of 26
66794	glycogen metabolism	60	3 of 5
66794	phenylacetate degradation (aerobic)	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	tryptophan metabolism	57.89	22 of 38
66794	glutathione metabolism	57.14	8 of 14
66794	d-mannose degradation	55.56	5 of 9
66794	cysteine metabolism	55.56	10 of 18
66794	lysine metabolism	54.76	23 of 42
66794	urea cycle	53.85	7 of 13
66794	myo-inositol biosynthesis	50	5 of 10
66794	kanosamine biosynthesis II	50	1 of 2
66794	toluene degradation	50	2 of 4
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	carnitine metabolism	50	4 of 8
66794	glycine metabolism	50	5 of 10
66794	ethanol fermentation	50	1 of 2
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	ketogluconate metabolism	50	4 of 8
66794	selenocysteine biosynthesis	50	3 of 6
66794	cholesterol biosynthesis	45.45	5 of 11
66794	degradation of hexoses	44.44	8 of 18
66794	lipid A biosynthesis	44.44	4 of 9
66794	daunorubicin biosynthesis	44.44	4 of 9
66794	nitrate assimilation	44.44	4 of 9
66794	androgen and estrogen metabolism	43.75	7 of 16
66794	tyrosine metabolism	42.86	6 of 14
66794	propanol degradation	42.86	3 of 7
66794	cardiolipin biosynthesis	42.86	3 of 7
66794	3-chlorocatechol degradation	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	phenol degradation	40	8 of 20
66794	gallate degradation	40	2 of 5
66794	degradation of pentoses	39.29	11 of 28
66794	d-xylose degradation	36.36	4 of 11
66794	bile acid biosynthesis, neutral pathway	35.29	6 of 17
66794	sphingosine metabolism	33.33	2 of 6
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	arachidonic acid metabolism	33.33	6 of 18
66794	3-phenylpropionate degradation	33.33	5 of 15
66794	IAA biosynthesis	33.33	1 of 3
66794	4-hydroxymandelate degradation	33.33	3 of 9
66794	sulfoquinovose degradation	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	chlorophyll metabolism	33.33	6 of 18
66794	cyanate degradation	33.33	1 of 3
66794	sulfate reduction	30.77	4 of 13
66794	coenzyme M biosynthesis	30	3 of 10
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	polyamine pathway	26.09	6 of 23
66794	lactate fermentation	25	1 of 4
66794	CMP-KDO biosynthesis	25	1 of 4
66794	ascorbate metabolism	22.73	5 of 22

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Built environment	#Clean room

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
17435	pharmaceutical clean room	Sachsen-Anhalt	Germany	DEU	Europe
67770	Pharmaceutical clean room

Taxonmaps

69479

Global distribution of 16S sequence FR832425 (>99% sequence identity) for Naumannella halotolerans subclade from Microbeatlas

Aquatic Samples	58
Soil Samples	51
Animal Samples	173
Plant Samples	7
Total Samples	289

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
17435	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM436464v1 assembly for Naumannella halotolerans DSM 24323	contig	GCA_004364645	993414.3	2731957515	993414	74.18

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17435	Naumannella halotolerans partial 16S rRNA gene, type strain WS4616T	FR832425	1407		993414

GC content

@ref	GC-content (mol%)	Method
17435	67.7	high performance liquid chromatography (HPLC)
30487	67.7

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM436464v1 assembly for Naumannella halotolerans DSM 24323
NCBI: GCA_004364645

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.94	no
125439	gram_stain	BacteriaNetⓘ	positive	89.80	no
125439	spore_formation	BacteriaNetⓘ	no	77.61	no
125439	motility	BacteriaNetⓘ	no	81.98	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Naumannella halotolerans strain DSM 24323
PATRIC: 993414.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.51	yes
125438	anaerobic	anaerobicⓘ	no	98.16	yes
125438	aerobic	aerobicⓘ	yes	90.60	yes
125438	spore-forming	spore-formingⓘ	no	77.93	yes
125438	thermophilic	thermophileⓘ	no	95.50	no
125438	flagellated	motile2+ⓘ	no	96.49	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Naumannella cuiyingiana sp. nov., isolated from a ca. 1500-year-old mural painting, and emended description of the genus Naumannella.	Tian T, Wu F, Ma Y, Xiang T, Ma W, Hu W, Wu G, An L, Wang W, Feng H	Int J Syst Evol Microbiol	10.1099/ijsem.0.001964	2017
Phylogeny	Naumannella huperziae sp. nov., an endophytic actinobacterium isolated from Huperzia serrata (Thunb.).	Sun Y, Chen HH, Sun HM, Ai MJ, Su J, Yu LY, Zhang YQ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001882	2017
Phylogeny	Naumannella halotolerans gen. nov., sp. nov., a Gram-positive coccus of the family Propionibacteriaceae isolated from a pharmaceutical clean room and from food.	Rieser G, Scherer S, Wenning M	Int J Syst Evol Microbiol	10.1099/ijs.0.037218-0	2012

References

#17435	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24323
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26820	IJSEM 3042 2012 ( DOI 10.1099/ijs.0.037218-0 , PubMed 22307502 )
#30487	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26820
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Naumannella halotolerans (DSM 24323, LMG 26184, WS 4616)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_004364645

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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