Name: Brooklawnia cerclae BL-34
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12669

Type strain:

Species: Brooklawnia cerclae

Strain Designation: BL-34

Culture col. no.: DSM 19609, LMG 23248, NRRL B-41418, CCUG 55599, JCM 14918, NBRC 106143

Strain history: LMG 23248 <-- W. M. Moe BL-34 <-- H.-S. Bae.

NCBI tax ID(s): 349934 (species)

SI-ID 282000

LMG 23248, NRRL B-41418, DSM 19609, JCM 14918, CCUG 55599

Section

Brooklawnia cerclae BL-34 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from groundwater, contaminated by chlorosolvents and petrolium hydrocarbons.

Gram-positive
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Propionibacteriaceae*
	Genus	*Brooklawnia*
	Species	**Brooklawnia cerclae**
	Full Scientific Name (LPSN)	Brooklawnia cerclae Rainey et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31802]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #31802]	Cell shape	rod-shaped

[Ref.: #31802]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %92.435

[Ref.: #31802]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8126]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #8126]

Culture medium growth

[Ref.: #8126]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8126]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8126]	growth	37 °C
[Ref.: #31802]	growth	10-40 °C
[Ref.: #31802]	optimum	37 °C
[Ref.: #60665]	growth	37 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #8126]	Temperature range	mesophilic
[Ref.: #31802]	Temperature range	mesophilic
[Ref.: #60665]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31802]			optimum	6.5
[Ref.: #31802]			growth	4.5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8126]	Oxygen tolerance	anaerobe
[Ref.: #31802]	Oxygen tolerance	facultative anaerobe
[Ref.: #60665]	Oxygen tolerance	anaerobe
[Ref.: #60665]	Oxygen tolerance	microaerophile

[Ref.: #31802]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.981

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31802]		NaCl		growth	0-3	%

[Ref.: #31802]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31802]	arabinose ChEBI	+	carbon source
[Ref.: #31802]	fructose ChEBI	+	carbon source
[Ref.: #31802]	galactose ChEBI	+	carbon source
[Ref.: #31802]	glucose ChEBI	+	carbon source
[Ref.: #31802]	glycerol ChEBI	+	carbon source
[Ref.: #31802]	glycogen ChEBI	+	carbon source
[Ref.: #31802]	lactate ChEBI	+	carbon source
[Ref.: #31802]	maltose ChEBI	+	carbon source
[Ref.: #31802]	mannitol ChEBI	+	carbon source
[Ref.: #31802]	mannose ChEBI	+	carbon source
[Ref.: #31802]	pyruvate ChEBI	+	carbon source
[Ref.: #31802]	rhamnose ChEBI	+	carbon source
[Ref.: #31802]	ribose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #31802]		catalase	+	1.11.1.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8126]	Sample type/isolated from	groundwater, contaminated by chlorosolvents and petrolium hydrocarbons
[Ref.: #8126]	Geographic location (country and/or sea, region)	Baton Rouge Louisiana
[Ref.: #8126]	Country	USA
[Ref.: #8126]	Country ISO 3 Code	USA
[Ref.: #8126]	Continent	North America

[Ref.: #60665]	Sample type/isolated from	Chlorosolvent-contaminated anaerobic groundwater
[Ref.: #60665]	Geographic location (country and/or sea, region)	Brooklawn,well W-1024-1
[Ref.: #60665]	Country	USA
[Ref.: #60665]	Country ISO 3 Code	USA
[Ref.: #60665]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Chlorosolvent-contaminated anaerobic groundwater
[Ref.: #67770]	Geographic location (country and/or sea, region)	LA
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered	#Contamination	-
#Environmental	#Aquatic	#Groundwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ196625 (>99% sequence identity) for Brooklawnia cerclae subclade from Microbeatlas

Aquatic Samples	723
Soil Samples	36
Animal Samples	323
Plant Samples	15
Total Samples	1097

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8126]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8126]	Brooklawnia cerclae strain BL-34 16S ribosomal RNA gene, partial sequence	DQ196625	1459	ENA	349934 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Brooklawnia cerclae DSM 19609	GCA_011758645	contig	GenBank	349934 tax ID
[Ref.: #66792]	Brooklawnia cerclae strain DSM 19609	349934.3	wgs	PATRIC	349934 tax ID	*
[Ref.: #66792]	Brooklawnia cerclae DSM 19609	2820979626	draft	JGI IMG/M	349934 tax ID	*

[Ref.: #8126]	GC-content	67.5 mol%
[Ref.: #31802]	GC-content	67.5-67.9 mol%
[Ref.: #67770]	GC-content	67.5 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Brooklawnia cerclae strain DSM 19609 PATRIC: 349934.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Brooklawnia cerclae strain DSM 19609 PATRIC: 349934.3
[Ref.: #69480]	gram-positive	yes	83.348	no
[Ref.: #69480]	anaerobic	no	74.905	no
[Ref.: #69480]	halophile	no	93.891	no
[Ref.: #69480]	spore-forming	no	92.746	yes
[Ref.: #69480]	glucose-util	yes	85.97	no
[Ref.: #69480]	motile	no	89.776	no
[Ref.: #69480]	flagellated	no	97.745	yes
[Ref.: #69480]	aerobic	no	60.665	no
[Ref.: #69480]	thermophile	no	97.982	yes
[Ref.: #69480]	glucose-ferment	yes	52.155	no

Availability in culture collections External links

[Ref.: #8126]

Culture collection no.

DSM 19609, LMG 23248, NRRL B-41418, CCUG 55599, JCM 14918, NBRC 106143

[Ref.: #81880]

SI-ID 282000

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brooklawnia cerclae gen. nov., sp. nov., a propionate-forming bacterium isolated from chlorosolvent-contaminated groundwater.	Bae HS, Moe WM, Yan J, Tiago I, da Costa MS, Rainey FA	Int J Syst Evol Microbiol	10.1099/ijs.0.64317-0	2006	*

References

#8126

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19609

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31802

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28071 (see below)

#60665 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55599

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#81880

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID282000.1 )

#28071

IJSEM 1977 2006 ( DOI 10.1099/ijs.0.64317-0 , PubMed 16902040 )

* These data were automatically processed and therefore are not curated

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Brooklawnia cerclae strain DSM 19609

Brooklawnia cerclae strain DSM 19609

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