Brooklawnia cerclae (DSM 19609, LMG 23248, NRRL B-41418)

Name: Brooklawnia cerclae (DSM 19609, LMG 23248, NRRL B-41418)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12669

Type strain

Strain Designation BL-34

Culture col. no. DSM 19609 LMG 23248 NRRL B-41418 CCUG 55599 BL-34 2 more

NCBI tax ID(s) 349934

Special Collections DSMZ CCUG JCM Literature strains

Links

Brooklawnia cerclae BL-34 is an anaerobe, mesophilic, Gram-positive prokaryote that was isolated from groundwater, contaminated by chlorosolvents and petrolium hydrocarbons.

Gram-positive rod-shaped anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 282000

LMG 23248,NRRL B-41418,DSM 19609,JCM 14918,CCUG 55599

@ref 20215

Last LPSN update 2025-12-16 09:49:54

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Propionibacteriales

Family Propionibacteriaceae

Genus Brooklawnia

Species Brooklawnia cerclae

Full scientific name Brooklawnia cerclae Rainey et al. 2006

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
31802	positive	rod-shaped
125438				92

Pigmentation

31802

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
8126	PYG MEDIUM (MODIFIED) (DSMZ Medium 104)		Medium recipe at MediaDive	Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8126	positive	growth	37	mesophilic
31802	positive	growth	10-40
31802	positive	optimum	37	mesophilic
60665	positive	growth	37	mesophilic
67770	positive	growth	28	mesophilic

Culture pH

@ref	Ability	Type	PH
31802	positive	optimum	6.5
31802	positive	growth	4.5-8

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
8126	anaerobe
31802	facultative anaerobe
60665	anaerobe
60665	microaerophile

Spore formation

@ref	Spore formation	Confidence
31802
125439		90

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31802	NaCl	positive	growth	0-3 %

Observation

@ref	Observation
31802	aggregates in chains
67770	quinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31802	22599 ChEBI	arabinose	+	carbon source
31802	28757 ChEBI	fructose	+	carbon source
31802	28260 ChEBI	galactose	+	carbon source
31802	17234 ChEBI	glucose	+	carbon source
31802	17754 ChEBI	glycerol	+	carbon source
31802	28087 ChEBI	glycogen	+	carbon source
31802	24996 ChEBI	lactate	+	carbon source
31802	17306 ChEBI	maltose	+	carbon source
31802	29864 ChEBI	mannitol	+	carbon source
31802	37684 ChEBI	mannose	+	carbon source
31802	15361 ChEBI	pyruvate	+	carbon source
31802	26546 ChEBI	rhamnose	+	carbon source
31802	33942 ChEBI	ribose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
31802	catalase	+	1.11.1.6

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Contamination	-
#Environmental	#Aquatic	#Groundwater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
8126	groundwater, contaminated by chlorosolvents and petrolium hydrocarbons	Baton Rouge Louisiana	USA	USA	North America
60665	Chlorosolvent-contaminated anaerobic groundwater	Brooklawn,well W-1024-1	USA	USA	North America
67770	Chlorosolvent-contaminated anaerobic groundwater	LA	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence DQ196625 (>99% sequence identity) for Brooklawnia cerclae subclade from Microbeatlas

Aquatic Samples	723
Soil Samples	36
Animal Samples	323
Plant Samples	15
Total Samples	1097

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8126	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM1175864v1 assembly for Brooklawnia cerclae DSM 19609	contig	GCA_011758645	349934.3	2820979626	349934	74.68
124043	ASM4242886v1 assembly for Brooklawnia cerclae JCM 14918	scaffold	GCA_042428865			349934	70.62
124043	ASM3953046v1 assembly for Brooklawnia cerclae JCM 14918	scaffold	GCA_039530465			349934	67.42

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
8126	Brooklawnia cerclae strain BL-34 16S ribosomal RNA gene, partial sequence	DQ196625	1459		349934

GC content

@ref	GC-content (mol%)	Method
8126	67.5
31802	67.5-67.9
67770	67.5	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Brooklawnia cerclae DSM 19609
NCBI: GCA_011758645

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	90.00	no
125439	motility	BacteriaNetⓘ	yes	55.80	no
125439	gram_stain	BacteriaNetⓘ	positive	81.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	71.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Brooklawnia cerclae strain DSM 19609
PATRIC: 349934.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.51	no
125438	anaerobic	anaerobicⓘ	no	75.24	no
125438	spore-forming	spore-formingⓘ	no	73.08	yes
125438	aerobic	aerobicⓘ	no	55.39	no
125438	thermophilic	thermophileⓘ	no	93.88	yes
125438	flagellated	motile2+ⓘ	no	92.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brooklawnia cerclae gen. nov., sp. nov., a propionate-forming bacterium isolated from chlorosolvent-contaminated groundwater.	Bae HS, Moe WM, Yan J, Tiago I, da Costa MS, Rainey FA	Int J Syst Evol Microbiol	10.1099/ijs.0.64317-0	2006

References

#8126	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19609
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#31802	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28071 (see below)
#60665	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55599
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Brooklawnia cerclae (DSM 19609, LMG 23248, NRRL B-41418)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_011758645

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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